scholarly journals Candidemia among Iranian Patients with Severe COVID-19 Admitted to ICUs

2021 ◽  
Vol 7 (4) ◽  
pp. 280
Author(s):  
Amir Arastehfar ◽  
Tahmineh Shaban ◽  
Hossein Zarrinfar ◽  
Maryam Roudbary ◽  
Mona Ghazanfari ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Clinical Importance ◽  
Rapid Diagnosis ◽  
Rhodotorula Mucilaginosa ◽  
Chain Reaction ◽  
Timely Initiation ◽  
Polymerase Chain ◽  
Severity Of The Disease ◽  
Iranian Patients

As a novel risk factor, COVID-19 has led to an increase in the incidence of candidemia and an elevated mortality rate. Despite being of clinical importance, there is a lack of data regarding COVID-19-associated candidemia (CAC) among Iranian patients. Therefore, in this retrospective study, we assessed CAC epidemiology in the intensive care units (ICUs) of two COVID-19 centers in Mashhad, Iran, from early November 2020 to late January 2021. Yeast isolates from patients’ blood were identified by 21-plex polymerase chain reaction (PCR) and sequencing, then subjected to antifungal susceptibility testing according to the CLSI M27-A3 protocol. Among 1988 patients with COVID-19 admitted to ICUs, seven had fungemia (7/1988; 0.03%), among whom six had CAC. The mortality of the limited CAC cases was high and greatly exceeded that of patients with COVID-19 but without candidemia (100% (6/6) vs. 22.7% (452/1988)). In total, nine yeast isolates were collected from patients with fungemia: five Candida albicans, three C. glabrata, and one Rhodotorula mucilaginosa. Half of the patients infected with C. albicans (2/4) were refractory to both azoles and echinocandins. The high mortality of patients with CAC, despite antifungal therapy, reflects the severity of the disease in these patients and underscores the importance of rapid diagnosis and timely initiation of antifungal treatment.

scholarly journals Itraconazole-resistant Candida auris with phospholipase, proteinase and hemolysin activity from a case of vulvovaginitis

2015 ◽  
Vol 9 (04) ◽  
pp. 435-437 ◽  
Author(s):  
Dharmendra Kumar ◽  
Tuhina Banerjee ◽  
Chandra Bhan Pratap ◽  
Ragini Tilak
Keyword(s):  
Candida Species ◽  
Internal Transcribed Spacer ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Emerging Pathogen ◽  
Chain Reaction ◽  
Candida Auris ◽  
Candida Spp ◽  
Oral Fluconazole ◽  
Polymerase Chain

Since the emergence of pathogenic non-albicans Candida species, a number of new isolates have been added to the list. One such unusual species is Candida auris (C. auris), recently isolated and studied in few reports. In this study, a case of vulvovaginitis caused by Candida auris incidentally identified by molecular methods using internal transcribed spacer polymerase chain reaction (ITS PCR) is described. Antifungal susceptibility testing revealed the isolate to be resistant to itraconazole (MIC ≥ 2 µg/ml) and expressed important virulence factors including phospholipase, proteinase and hemolysin activity. The patient was successfully treated with oral fluconazole and did not have any invasive fungemia. Very few cases of this emerging pathogen have been reported. However, its isolation from clinical specimens reveals the significance of non-albicans candida species over C. albicans and the diversity of Candida spp causing infections.

scholarly journals A comparative evaluation of two chromogenic media for surveillance of carbapenem-resistant Enterobacterales with non-carbapenemase-producing or carbapenemase-producing strains other than blaKPC: blaGES-5, blaNDM-1 or blaVIM-2

2019 ◽  
Vol 43 (3) ◽  
pp. 173-176
Author(s):  
Chang-Hun Park
Keyword(s):  
Rapid Detection ◽  
Comparative Evaluation ◽  
Susceptibility Testing ◽  
Rapid Identification ◽  
Surveillance Program ◽  
Contact Precaution ◽  
Chain Reaction ◽  
Carbapenem Resistant ◽  
Control And Prevention ◽  
Polymerase Chain

Abstract Background Infections caused by carbapenem-resistant Enterobacterales (CREs) are an emerging problem associated with high rates of morbidity and mortality. CREs are divided into two categories (carbapenemase-producing [CP] CRE and non-CP CRE). The most prevalent carbapenemase produced by Enterobacterales is Klebsiella pneumoniae carbapenemase (KPC) in Korea. Rapid identification of CREs is clinically important in infection control precaution. We compared the performance of two chromogenic media (chromID CARBA agar and CHROMagar KPC agar) for non-CP CREs or CP CREs with blaGES-5, blaNDM-1 or blaVIM-2 in a Korean hospital. Methods The study was carried out during a 3-month period from April to June 2017 during the surveillance program for CRE colonization. Antimicrobial susceptibility testing (AST) and polymerase chain reaction (PCR) were performed at the Korean Centers for Disease Control and Prevention. Results A total of 45 rectal swabs from 42 hospitalized patients were examined. Sensitivity of both chromID CARBA and CHROMagar KPC were 100% for CP CREs; and 50% and 100% for non-CP CREs, respectively. Specificity of chromID CARBA and CHROMagar KPC were 89.2% and 70.3% for CP CRE, respectively; and 76.9% and 66.7% for non-CP CRE, respectively. Conclusions The CHROMagar KPC is useful to monitor non-CP and CP CREs. The chromID CARBA is efficient for rapid detection of CP CREs requiring high contact precaution.

scholarly journals Rapid diagnosis of vesicular stomatitis virus in Ecuador by the use of polymerase chain reaction

2007 ◽  
Vol 38 (3) ◽  
pp. 500-506 ◽  
Author(s):  
Lya Madureira Sepúlveda ◽  
Viviana Malirat ◽  
Ingrid E. Bergmann ◽  
Anibal Mantilla ◽  
Elmiro Rosendo do Nascimento
Keyword(s):  
Polymerase Chain Reaction ◽  
Vesicular Stomatitis Virus ◽  
Rapid Diagnosis ◽  
Chain Reaction ◽  
Vesicular Stomatitis ◽  
Polymerase Chain

scholarly journals Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction

1998 ◽  
Vol 32 ◽  
pp. 87-90 ◽  
Author(s):  
S Oshima ◽  
JI Hata ◽  
N Hirasawa ◽  
T Ohtaka ◽  
I Hirono ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Red Sea ◽  
Rapid Diagnosis ◽  
Sea Bream ◽  
Red Sea Bream ◽  
Chain Reaction ◽  
Polymerase Chain
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