scholarly journals Ethanol Production from Wheat Straw Hydrolysate by Issatchenkia Orientalis Isolated from Waste Cooking Oil

2021 ◽  
Vol 7 (2) ◽  
pp. 121
Author(s):  
Alexander Zwirzitz ◽  
Lauren Alteio ◽  
Daniel Sulzenbacher ◽  
Michael Atanasoff ◽  
Manuel Selg
Keyword(s):  
Ethanol Production ◽  
Wheat Straw ◽  
Elevated Temperatures ◽  
Value Added ◽  
Waste Cooking Oil ◽  
Lignocellulosic Materials ◽  
Fermentation Inhibitors ◽  
Issatchenkia Orientalis ◽  
Cooking Oil ◽  
Wheat Straw Hydrolysate

The interest in using non-conventional yeasts to produce value-added compounds from low cost substrates, such as lignocellulosic materials, has increased in recent years. Setting out to discover novel microbial strains that can be used in biorefineries, an Issatchenkia orientalis strain was isolated from waste cooking oil (WCO) and its capability to produce ethanol from wheat straw hydrolysate (WSHL) was analyzed. As with previously isolated I. orientalis strains, WCO-isolated I. orientalis KJ27-7 is thermotolerant. It grows well at elevated temperatures up to 42 °C. Furthermore, spot drop tests showed that it is tolerant to various chemical fermentation inhibitors that are derived from the pre-treatment of lignocellulosic materials. I. orientalis KJ27-7 is particularly tolerant to acetic acid (up to 75 mM) and tolerates 10 mM formic acid, 5 mM furfural and 10 mM hydroxymethylfurfural. Important for biotechnological cellulosic ethanol production, I. orientalis KJ27-7 grows well on plates containing up to 10% ethanol and media containing up to 90% WSHL. As observed in shake flask fermentations, the specific ethanol productivity correlates with WSHL concentrations. In 90% WSHL media, I. orientalis KJ27-7 produced 10.3 g L−1 ethanol within 24 h. This corresponds to a product yield of 0.50 g g−1 glucose (97% of the theoretical maximum) and a volumetric productivity of 0.43 g L−1 h−1. Therefore, I. orientalis KJ27-7 is an efficient producer of lignocellulosic ethanol from WSHL.

Detoxification of Wheat Straw Formic Acid Hydrolysis and Xylitol Production

2011 ◽  
Vol 383-390 ◽  
pp. 5453-5457 ◽  
Author(s):  
Jun Ping Zhuang ◽  
Lu Lin ◽  
Sun Yong ◽  
Chun Sheng Pang
Keyword(s):  
Ion Exchange ◽  
Formic Acid ◽  
Acid Hydrolysis ◽  
Wheat Straw ◽  
Exchange Resin ◽  
Reaction System ◽  
Ion Exchange Resin ◽  
Fermentation Inhibitors ◽  
Formic Acid Hydrolysis ◽  
Wheat Straw Hydrolysate

Xylitol can be obtained from hemicelullosic fraction of lignocellulosic materials containing D-xylose. Formic acid hydrolysis is widely used in lignocellulose pretreatment. However, formic acid hydrolysis wheat straw cannot be directly used as fermentation subsequently owing to various fermentation inhibitors, especially the formic acid in reaction system and released during pretreatment. This study describes main hydrolysis components and inhibitors prepared by hydrolysis of wheat straw with formic acid solution, and different detoxification methods ware used to romve the inhibitors. Study found treatment of wheat straw hydrolysate with overliming treatment and D311 ion-exchange resin had a good result on reduction formic acid and other main inhibitors, results showed which can eliminate 94% of residual formic acid and with only 15% reducion of xylose. To verify the effectiveness of different detoxification methods, hydrolysates of detoxification werr fermented by Candida.tropicalis AS2.1776, and the results also found that the hydrolysate treatmented with overliming treatment and D311 ion-exchange resin have the hightest xylitol yield.

scholarly journals Metabolic engineering of Aspergillus niger via ribonucleoprotein-based CRISPR–Cas9 system for succinic acid production from renewable biomass

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Lei Yang ◽  
Mikkel Møller Henriksen ◽  
Rasmus Syrach Hansen ◽  
Mette Lübeck ◽  
Jesper Vang ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Succinic Acid ◽  
Wheat Straw ◽  
Acid Production ◽  
Value Added ◽  
Fungal Cell ◽  
Renewable Biomass ◽  
Succinic Acid Production ◽  
Wheat Straw Hydrolysate ◽  
Low Culture

Abstract Background Succinic acid has great potential to be a new bio-based building block for deriving a number of value-added chemicals in industry. Bio-based succinic acid production from renewable biomass can provide a feasible approach to partially alleviate the dependence of global manufacturing on petroleum refinery. To improve the economics of biological processes, we attempted to explore possible solutions with a fungal cell platform. In this study, Aspergillus niger, a well-known industrial production organism for bio-based organic acids, was exploited for its potential for succinic acid production. Results With a ribonucleoprotein (RNP)-based CRISPR–Cas9 system, consecutive genetic manipulations were realized in engineering of the citric acid-producing strain A. niger ATCC 1015. Two genes involved in production of two byproducts, gluconic acid and oxalic acid, were disrupted. In addition, an efficient C4-dicarboxylate transporter and a soluble NADH-dependent fumarate reductase were overexpressed. The resulting strain SAP-3 produced 17 g/L succinic acid while there was no succinic acid detected at a measurable level in the wild-type strain using a synthetic substrate. Furthermore, two cultivation parameters, temperature and pH, were investigated for their effects on succinic acid production. The highest amount of succinic acid was obtained at 35 °C after 3 days, and low culture pH had inhibitory effects on succinic acid production. Two types of renewable biomass were explored as substrates for succinic acid production. After 6 days, the SAP-3 strain was capable of producing 23 g/L and 9 g/L succinic acid from sugar beet molasses and wheat straw hydrolysate, respectively. Conclusions In this study, we have successfully applied the RNP-based CRISPR–Cas9 system in genetic engineering of A. niger and significantly improved the succinic acid production in the engineered strain. The studies on cultivation parameters revealed the impacts of pH and temperature on succinic acid production and the future challenges in strain development. The feasibility of using renewable biomass for succinic acid production by A. niger has been demonstrated with molasses and wheat straw hydrolysate.

scholarly journals Production and added value of waste cooking oil product derivatives in the Bali Province

2021 ◽  
Vol 4 (1) ◽  
pp. 56-62
Author(s):  
Dewa Ayu Anom Yuarini ◽  
Ganda Putra ◽  
AAPA Suryawan Wiranatha
Keyword(s):  
Economic Value ◽  
Value Added ◽  
Waste Cooking Oil ◽  
Added Value ◽  
Oil Consumption ◽  
Liquid Soap ◽  
Cooking Oil ◽  
The Government ◽  
Derivative Products ◽  
Value Added Products

Waste cooking oil or waste cooking oil (WCO) is the remaining oil (or by-products) produced during food frying. In 2019, the cooking oil consumption in Bali Province was 4,735,057 L/month and the WCO produced was estimated about 3,314,540 L/month. The Government of Bali plans to manage WCO in an integrated manner WCO in an integrated manner by processing it as derivative products with economic value. The study aimed to transform WCO into high-value added products (i.e. aromatherapy candles, liquid soap, and biodiesel), and to analyze their economy valued added. The Hayami method was used to determine the economyvalue added of each product. The results showed that WCO was most widely used in producing biodiesel (84%), candle (17%), and liquid soap (10%). Based on the Hayami method, the added value from candle products was IDR. 4,838 / kg (or added value ratio of 9.68%), was classified as a low added product. The added value of liquid soap was IDR 8,495/kg (or added value ratio of 47.38%), classified as a high added value product. While, biodiesel products generating the added value of IDR 2,363/kg (or added value ratio of 25.57%), classified as a medium added value product.

scholarly journals Evaluating Lignin-Rich Residues from Biochemical Ethanol Production of Wheat Straw and Olive Tree Pruning by FTIR and 2D-NMR

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
José I. Santos ◽  
Raquel Martín-Sampedro ◽  
Úrsula Fillat ◽  
José M. Oliva ◽  
María J. Negro ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Wheat Straw ◽  
Internal Energy ◽  
Energy Use ◽  
Value Added ◽  
Olive Tree ◽  
2D Nmr ◽  
Cellulosic Biomass ◽  
G Ratio ◽  
Tree Pruning

Lignin-rich residues from the cellulose-based industry are traditionally incinerated for internal energy use. The future biorefineries that convert cellulosic biomass into biofuels will generate more lignin than necessary for internal energy use, and therefore value-added products from lignin could be produced. In this context, a good understanding of lignin is necessary prior to its valorization. The present study focused on the characterization of lignin-rich residues from biochemical ethanol production, including steam explosion, saccharification, and fermentation, of wheat straw and olive tree pruning. In addition to the composition and purity, the lignin structures (S/G ratio, interunit linkages) were investigated by spectroscopy techniques such as FTIR and 2D-NMR. Together with the high lignin content, both residues contained significant amounts of carbohydrates, mainly glucose and protein. Wheat straw lignin showed a very low S/G ratio associated withp-hydroxycinnamates (p-coumarate and ferulate), whereas a strong predominance of S over G units was observed for olive tree pruning lignin. The main interunit linkages present in both lignins wereβ-O-4′ethers followed by resinols and phenylcoumarans. These structural characteristics determine the use of these lignins in respect to their valorization.

scholarly journals Improving the fermentability of pre-hydrolysis liquor to produce ethanol and xylonic acid by removing fermentation inhibitors using polystyrene divinylbenzene resin

2020 ◽  
Author(s):  
Caoxing Huang ◽  
Wenqian Lin ◽  
Yuxuan Shi ◽  
Yayue Zheng ◽  
Qiang Yong
Keyword(s):  
Ethanol Production ◽  
Acid Hydrolysis ◽  
Value Added ◽  
Fermentation Inhibitors ◽  
Waste Streams ◽  
Xylonic Acid ◽  
Soluble Lignin ◽  
Xylose Concentration ◽  
Resin Treatment ◽  
Value Added Products

Abstract Background The existences of soluble lignin, furfural and HMF in industrial pre-hydrolysis liquor (PHL) can inhibit its bioconversion into bioethanol and other bio-chemicals. Although various technologies have been carried out to remove these inhibitors, a undesirable sugars removal is also happened during the process. Hence, polystyrene divinylbenzene (PS-DVB) resin was used as adsorptive material to simultaneously remove fermentation inhibitors while retain sugars with higher yield for improving the fermentability of PHL after acid-hydrolysis (A-PHL) treatment. The fermentability of treated PHL was evaluated by the bioconversion into ethanol and xylosic acid (XA). Results Results showed that an highest xylose concentration (101.1 g/L) in PHL could be obtained by acid-hydrolysis at 100 oC for 80 min with 4% acid, while concentration of fermentation inhibitors (furfural, HMF and lignin) could also be significantly improved. After treating, not only 97% of lignin, 92% of furfural, and 97% of HMF were removed from solution, but also 96% of xylose was retained for subsequent fermentation. With resin treatment, the fermentability of A-PHL could be improved by 162–282% for ethanol production from A-PHL with 30–50 g/L xylose and by 18–828% for XA production from A-PHL with 90–150 g/L xylose. Conclusions These results confirmed that PS-DVB resin can remove inhibitors from PHL before producing value-added products by bioconversion. In addition, the work will ideally provide a concept by which pulping facilities could go about producing value-added chemicals from existing waste streams.

scholarly journals Eco-efficiency analysis of waste cooking oil recycling into liquid dish soap using life cycle assessment

2021 ◽  
Vol 896 (1) ◽  
pp. 012066
Author(s):  
S Hartini ◽  
Y Widharto ◽  
S R Indarto ◽  
G Murdikaningrum
Keyword(s):  
Life Cycle Assessment ◽  
Life Cycle ◽  
Unsaturated Fatty Acids ◽  
Value Added ◽  
Efficiency Index ◽  
Waste Cooking Oil ◽  
Economic Potential ◽  
Recycling Process ◽  
Cooking Oil ◽  
Cooling Method

Abstract Waste Cooking Oil (WCO) is an oil with a chemical composition containing carcinogenic compounds formed during frying and unsaturated fatty acids. Improper handling of WCO can cause environmental pollution, especially water and soil. However, several studies have provided information that WCO can be recycled into products that are more value-added and have economic potential. Previous research has succeeded in finding the best combination of treatments for making soap from waste cooking oil that meets the standards of SNI 06-2048-1990, namely with a concentration of 22.5% KOH, cooling method and adsorption of activated charcoal and kepok banana peels. This study intends to analyze the environmental impact if WCO is recycled into liquid dish soap. The analysis was carried out using Life Cycle Assessment with the help of simapro software. The recycling process has the most significant impact on indicators of climate change and acidification. The eco-efficiency index is included in the affordable and sustainable categories.

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