scholarly journals Fungal Endophytes for Grass Based Bioremediation: An Endophytic Consortium Isolated from Agrostis stolonifera Stimulates the Growth of Festuca arundinacea in Lead Contaminated Soil

2020 ◽  
Vol 6 (4) ◽  
pp. 254
Author(s):  
Erika Soldi ◽  
Catelyn Casey ◽  
Brian R. Murphy ◽  
Trevor R. Hodkinson
Keyword(s):  
Heavy Metal ◽  
Contaminated Soils ◽  
Festuca Arundinacea ◽  
Growth Parameters ◽  
Fungal Endophytes ◽  
Agrostis Stolonifera ◽  
Toxic Waste ◽  
Practical Applications ◽  
Natural Flora ◽  
Bacteria And Fungi

Bioremediation is an ecologically-friendly approach for the restoration of heavy metal-contaminated sites and can exploit environmental microorganisms such as bacteria and fungi. These microorganisms are capable of removing and/or deactivating pollutants from contaminated substrates through biological and chemical reactions. Moreover, they interact with the natural flora, protecting and stimulating plant growth in these harsh conditions. In this study, we isolated a group of endophytic fungi from Agrostis stolonifera grasses growing on toxic waste from an abandoned lead mine (up to 47,990 Pb mg/kg) and identified them using DNA sequencing (nrITS barcoding). The endophytes were then tested as a consortium of eight strains in a growth chamber experiment in association with the grass Festuca arundinacea at increasing concentrations of lead in the soil to investigate how they influenced several growth parameters. As a general trend, plants treated with endophytes performed better compared to the controls at each concentration of heavy metal, with significant improvements in growth recorded at the highest concentration of lead (800 galena mg/kg). Indeed, this set of plants germinated and tillered significantly earlier compared to the control, with greater production of foliar fresh and dry biomass. Compared with the control, endophyte treated plants germinated more than 1-day earlier and produced 35.91% more plant tillers at 35 days-after-sowing. Our results demonstrate the potential of these fungal endophytes used in a consortium for establishing grassy plant species on lead contaminated soils, which may result in practical applications for heavy metal bioremediation.

scholarly journals Recent Developments in Microbe–Plant-Based Bioremediation for Tackling Heavy Metal-Polluted Soils

2021 ◽  
Vol 12 ◽  
Author(s):  
Lala Saha ◽  
Jaya Tiwari ◽  
Kuldeep Bauddh ◽  
Ying Ma
Keyword(s):  
Heavy Metal ◽  
Contaminated Soils ◽  
Metal Tolerance ◽  
Chemical Reduction ◽  
High Energy ◽  
Toxic Waste ◽  
Polluted Soils ◽  
Factors Affecting ◽  
Bacteria And Fungi ◽  
Trained Manpower

Soil contamination with heavy metals (HMs) is a serious concern for the developing world due to its non-biodegradability and significant potential to damage the ecosystem and associated services. Rapid industrialization and activities such as mining, manufacturing, and construction are generating a huge quantity of toxic waste which causes environmental hazards. There are various traditional physicochemical techniques such as electro-remediation, immobilization, stabilization, and chemical reduction to clean the contaminants from the soil. However, these methods require high energy, trained manpower, and hazardous chemicals make these techniques costly and non-environment friendly. Bioremediation, which includes microorganism-based, plant-based, microorganism-plant associated, and other innovative methods, is employed to restore the contaminated soils. This review covers some new aspects and dimensions of bioremediation of heavy metal-polluted soils. The bioremediation potential of bacteria and fungi individually and in association with plants has been reviewed and critically examined. It is reported that microbes such as Pseudomonas spp., Bacillus spp., and Aspergillus spp., have high metal tolerance, and bioremediation potential up to 98% both individually and when associated with plants such as Trifolium repens, Helianthus annuus, and Vallisneria denseserrulata. The mechanism of microbe’s detoxification of metals depends upon various aspects which include the internal structure, cell surface properties of microorganisms, and the surrounding environmental conditions have been covered. Further, factors affecting the bioremediation efficiency and their possible solution, along with challenges and future prospects, are also discussed.

scholarly journals Quantitative and qualitative basement of microbial presence during phytoremediation of heavy metal polluted soil using Chromolaena odorata

2020 ◽  
Vol 31 (2) ◽  
pp. 145-151
Author(s):  
Gloria Omorowa Omoregie ◽  
Abraham Goodness Ogofure ◽  
Beckley Ikhajiagbe ◽  
Geoffrey Obinna Anoliefo
Keyword(s):  
Heavy Metal ◽  
Bacillus Subtilis ◽  
Plant Growth ◽  
Contaminated Soils ◽  
Plant Growth Promoting Rhizobacteria ◽  
Stem Cuttings ◽  
Chromolaena Odorata ◽  
Plant Growth Promoting ◽  
Bacteria And Fungi ◽  
Disturbed Soils

Abstract The presence and impact of bulk and rhizosphere microorganisms in contaminated soils can be huge, given that they have the ability to increase plants tolerance against abiotic stress, and also enhance plant growth, while supporting hastened remediation of disturbed soils. The present study quantitatively and qualitatively assessed presence of cultural fungi and bacteria during phytoremediation of heavy metal polluted soils using Chromolaena odorata. Stem cuttings of C. odorata were planted in soils polluted with Pb, Mn, Zn, Cd, and Cu at once (1ESC), thrice (3ESC) and five (5ESC) times their respective ecological screening concentrations (ESC). ESC of Pb, Mn and Zn is 50 mg/kg, Cd is 4 mg/kg, and Cu is 100 mg/kg. After 6 months, results showed that more than 10 species of bacteria and fungi were identified in the study, with P. aeruginosa and Bacillus subtilis being the most occurring bacteria while, Penicillium sp. and Aspergillus niger the most occurring fungi in both bulk and rhizospheric soils. The presence of known plant growth promoting rhizobacteria in plants rhizosphere including Azotobacter sp., Bacillus subtilis, B. pumilus, Clostridium sp., P. aeruginosa, and Klebsiella sp. was also reported.

scholarly journals Comparative Growth Response of Three Jatropha Species on Heavy Metal Contaminated Soil

2018 ◽  
Vol 5 (1) ◽  
pp. 26-32
Author(s):  
Olamilekan L Awotedu ◽  
Paul O Ogunbamowo ◽  
Bolajoko F Awotedu ◽  
Ileri-Oluwa B Emmanuel
Keyword(s):  
Heavy Metal ◽  
Plant Height ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Growth Parameters ◽  
Stem Diameter ◽  
Control Soil ◽  
Dump Site

This study investigated the comparative phytotoxcity effect of heavy metal contamination on Jatropha curcas, Jatropha gossypifolia and Jatropha multifida in contaminated soil from a dump site in Ibadan Nigeria. Seeds of J. curcas, J. gossypifolia and J. multifida were planted in a germination tray and later transplanted into polythene pots filled with 2kg of either control soil or heavy metal contaminated soil, a 3 × 2 factorial experiment laid out in complete randomized design (CRD) replicated four times was adopted; treatments imposed include T1 – J. curcas/Control Soil, T2 – J. gossypifolia/Control Soil, T3 – J. multifida/Control Soil, T4 – J. curcas/Contaminated soil, T5 – J. gossypifolia/Contaminated Soil, and T6 – J. multifida/Contaminated Soil. Weekly variation in growth parameters: the plant height, leave production and stem diameter were measured over the course of 12 weeks. The growth parameters were dependent on a combination of both specie type and level of heavy metal contamination of soil. J. multifida (T3) (36.93cm) performed best, comparable with J. gossypiifolia (T2) (34.1cm) after 12 weeks while J. multifida (T6) had the lowest mean plant height (7.23cm) which is not significantly (p<0.05) different from other species on the contaminated soil; for leave production, J. gossypiifolia (T2) produced the highest mean number of leaves (9.67) which is comparable with J. multifida (T3) (9.00) and less so with J. curcas (T1) (6.67) with significant leave losses on the contaminated soils after 12 weeks; variation in stem diameter shows that J. curcas (T1) had the highest stem girth (1.96 mm) which is comparable to the value obtained for J. curcas (T4) (1.95mm), while J. multifida (T6) had the lowest stem girth (1.09 mm). J. gossypiifolia (T2) and J. multifida (T3) had comparable stem girth of 1.57mm and 1.47mm respectively. Toxicity of heavy metals in the contaminated soil greatly affect the growth parameters of the Jatropha.

Efficiency of microbially assisted phytoremediation of heavy-metal contaminated soils

2018 ◽  
Vol 26 (3) ◽  
pp. 316-332 ◽  
Author(s):  
Katarzyna Hrynkiewicz ◽  
Michał Złoch ◽  
Tomasz Kowalkowski ◽  
Christel Baum ◽  
Bogusław Buszewski
Keyword(s):  
Heavy Metal ◽  
Contaminated Soils ◽  
Field Experiments ◽  
Cost Benefit ◽  
Model Systems ◽  
Future Challenges ◽  
Vitro Model ◽  
Complex Relationships ◽  
Bacteria And Fungi

Phytoremediation is the bioremediation of contaminated soils and waters by using plants and their associated microorganisms. Phytoremediation of heavy metal (HM)-contaminated soils is based on immobilization of metals in rhizosphere soil and roots (phytostabilization) and on mobilization, uptake, and transfer of metals into the aboveground plant organs (phytoextraction). In this review, we aimed to (i) discuss the fundamentals, potential, and limitations of plant-associated microorganisms (bacteria and fungi) to increase the efficiency of phytostabilization and phytoextraction of HM-contaminated soils and (ii) describe promising developments and future challenges to expanding their use. Controlled inoculations of plants with growth-promoting microorganisms can significantly increase their root growth, biomass production, and stress tolerance in HM-contaminated soils. A serious weakness of phytoremediation in general is the usually high and difficult to measure expenditure of time for successful completion. The bioconcentration factors (BCFs) and the translocation factors (TFs) are among the most important measures of the efficiency of phytoremediation. However, an overview of BCFs and TFs for a variety of combinations of plants with defined associated microorganisms is lacking. Moreover, the joint evaluation of model systems would allow an improved cost–benefit calculation of microbial inoculations in phytoremediation systems. For this purpose, the use of in vitro model systems is considered to be preferable to field experiments due to the savings in time and costs and the control of environmental conditions. However, the transferability of in vitro data to field conditions is limited. Currently, attention is focused on the use of artificial neural networks, mainly to avoid formulating any complex relationships between soil inputs (e.g., soil amendments, pH, carbon, nitrogen and hydrogen contents, electrical conductivity, and dissolved organic carbon) and design outputs (e.g., BCFs and TFs) beforehand and because of the high accuracy of the obtained models. The controlled use of associated microorganisms to increase the efficiency of phytoremediation of HM, mainly using combinations of Brassica and Salix spp. and rhizobacteria at contaminated soils, is a promising possibility. A crucial future challenge for the expansion of their use will be to develop well-defined cost- and time-efficient tools for a credible prediction of their effectiveness on contaminated field sites.

scholarly journals Interaction between Cd and Zn on Metal Accumulation, Translocation and Mineral Nutrition in Tall Fescue (Festuca arundinacea)

2019 ◽  
Vol 20 (13) ◽  
pp. 3332 ◽  
Author(s):  
Qin Dong ◽  
Shuai Hu ◽  
Ling Fei ◽  
Lijiao Liu ◽  
Zhaolong Wang
Keyword(s):  
Heavy Metal ◽  
Mineral Nutrition ◽  
Tall Fescue ◽  
Contaminated Soils ◽  
Festuca Arundinacea ◽  
Metal Accumulation ◽  
Xylem Sap ◽  
Mineral Elements ◽  
Mineral Nutrients ◽  
Cd Toxicity

Tall fescue (Festuca arundinacea), an accumulator that is able to accumulate and excrete cadmium (Cd), has attracted much attention for its possible use in phytoremediation of heavy metal contaminated soils. In the present study, the interaction between Cd and Zn, and their uptake, translocation and accumulation under external Cd and Zn treatment in tall fescue were investigated. The concentrations of K, Ca, Mg in xylem sap under Cd and Zn treatment were measured to determine the level of mineral nutrients and their relationship with Cd alleviation. The result showed that Cd and Zn antagonized each other in the roots, while Cd antagonized Zn and Zn synergized Cd in the shoots of tall fescue. Compared with Cd only treatment, the concentrations of Ca, Mg and K in xylem sap increased after the addition of Zn, and they increased the most in the guttation. This result indicated that the addition of Zn facilitates the level of mineral elements to alleviate Cd toxicity, which might be used to improve the phytoremediation efficiency of Cd contaminated soils by tall fescue.

scholarly journals Antimicrobial metabolite profiling of Nigrospora sphaerica from Adiantum philippense L.

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Kolathuru Puttamadaiah Ramesha ◽  
Nagabhushana Chandra Mohana ◽  
Bettadapura Rameshgowda Nuthan ◽  
Devaraju Rakshith ◽  
Sreedharamurthy Satish
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Xanthomonas Campestris ◽  
Fungal Endophytes ◽  
Leaf Segment ◽  
Spectroscopic Techniques ◽  
Biological Potential ◽  
Bioassay Guided Fractionation ◽  
Antimicrobial Metabolite ◽  
Bacteria And Fungi

Abstract Background Endophyte bestows beneficial aspects to its inhabiting host, along with a contribution to diverse structural attributes with biological potential. In this regard, antimicrobial profiling of fungal endophytes from medicinal plant Adiantum philippense revealed bioactive Nigrospora sphaerica from the leaf segment. Chemical and biological profiling through TLC–bioautography and hyphenated spectroscopic techniques confirmed the presence of phomalactone as an antimicrobial metabolite. Results The chemical investigation of the broth extract by bioassay-guided fractionation confirmed phomalactone as a bioactive antimicrobial secondary metabolite. The antimicrobial activity of phomalactone was found to be highest against Escherichia coli by disc diffusion assay. The MIC was found to be significant against both Escherichia coli and Xanthomonas campestris in the case of bacteria and dermatophyte Candida albicans at 150 μg/ml, respectively. Conclusions Overall, the results highlighted the antimicrobial potential of phomalactone from the endophyte Nigrospora sphaerica exhibiting a broad spectrum of antimicrobial activity against human and phytopathogenic bacteria and fungi. This work is the first report regarding the antibacterial activity of phomalactone.

scholarly journals Chemical Properties of Heavy Metal-Contaminated Soils from a Korean Military Shooting Range: Evaluation of Pb Sources Using Pb Isotope Ratios

2021 ◽  
Vol 11 (15) ◽  
pp. 7099
Author(s):  
Inkyeong Moon ◽  
Honghyun Kim ◽  
Sangjo Jeong ◽  
Hyungjin Choi ◽  
Jungtae Park ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Contaminated Soils ◽  
Management Strategies ◽  
Chemical Properties ◽  
Heavy Metal Concentration ◽  
Soil Samples ◽  
Shooting Range ◽  
Geochemical Properties ◽  
Mineral Phases

In this study, the geochemical properties of heavy metal-contaminated soils from a Korean military shooting range were analyzed. The chemical behavior of heavy metals was determined by analyzing the soil pH, heavy metal concentration, mineral composition, and Pb isotopes. In total, 24 soil samples were collected from a Korean military shooting range. The soil samples consist of quartz, albite, microcline, muscovite/illite, kaolinite, chlorite, and calcite. Lead minerals, such as hydrocerussite and anglesite, which are indicative of a transformation into secondary mineral phases, were not observed. All soils were strongly contaminated with Pb with minor concentrations of Cu, Ni, Cd, and Zn. Arsenic was rarely detected. The obtained results are indicated that the soils from the shooting range are contaminated with heavy metals and have evidences of different degree of anthropogenic Pb sources. This study is crucial for the evaluation of heavy metal-contaminated soils in shooting ranges and their environmental effect as well as for the establishment of management strategies for the mitigation of environmental risks.

scholarly journals A Short Review on Recent Advances of Hydrogel-Based Adsorbents for Heavy Metal Ions

Metals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 864
Author(s):  
Suguna Perumal ◽  
Raji Atchudan ◽  
Thomas Nesakumar Jebakumar Immanuel Edison ◽  
Rajendran Suresh Babu ◽  
Petchimuthu Karpagavinayagam ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Large Scale ◽  
Water Bodies ◽  
Dimensional Structure ◽  
High Porosity ◽  
Adsorption Method ◽  
Toxic Heavy Metal ◽  
Practical Applications

The growth of industry fulfills our necessity and promotes economic development. However, pollutants from such industries pollute water bodies which pose a high risk for living organisms. Thus, researchers have been urged to develop an efficient method to remove toxic heavy metal ions from water bodies. The adsorption method shows promising results for the removal of heavy metal ions and is easy to operate on a large scale, thus can be applied to practical applications. Numerous adsorbents were developed and reported, among them hydrogels, which attract great attention because of the reusability, ease of preparation, and handling. Hydrogels are generally prepared by the cross-linking of polymers that result in a three-dimensional structure, showing high porosity and high functionality. They are hydrophilic in nature because of the functional groups, and are non-toxic. Thus, this review provides various methods of hydrogel adsorbents preparation and summarizes recent progress in the use of hydrogel adsorbents for the removal of heavy metal ions. Further, the mechanism involved in the removal of heavy metal ions is briefly discussed. The most recent studies about the adsorption method for the treatment of heavy metal ions contaminated water are presented.

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