scholarly journals Influence of Environmental Growth Factors on the Biomass and Pigment Production of Chlorociboria aeruginascens

2019 ◽  
Vol 5 (2) ◽  
pp. 46 ◽  
Author(s):  
Stephanie Stange ◽  
Susanne Steudler ◽  
Hubertus Delenk ◽  
Anett Werner ◽  
Thomas Walther ◽  
...  
Keyword(s):  
Growth Factors ◽  
Organic Semiconductors ◽  
Ph Value ◽  
Fungal Growth ◽  
Soft Rot ◽  
Growth Conditions ◽  
Pigment Production ◽  
Biomass Growth ◽  
Oxygen Level ◽  
Green Pigment

The soft rot fungus Chlorociboria aeruginascens produces a blue–green pigment xylindein, which is of considerable interest for various applications such as in the veneer industry or in organic semiconductors. To understand the fungal growth as well as pigment production of C. aeruginascens, several studies were performed, the results of which are presented here. These studies investigated various growth conditions such as temperature, pH value, oxygen level and light intensity. It was observed that the formation of xylindein by C. aeruginascens decoupled from growth. In the primary metabolismus, the uncolored biomass is formed. Pigment production took place within the secondary metabolism, while biomass growth as well as pigment production depended on various growth conditions. It was also found that certain conditions encourage the switch in metabolism, leading to pigment production.

scholarly journals Influence of the Nutrients on the Biomass and Pigment Production of Chlorociboria aeruginascens

2019 ◽  
Vol 5 (2) ◽  
pp. 40 ◽  
Author(s):  
Stephanie Stange ◽  
Susanne Steudler ◽  
Hubertus Delenk ◽  
Anett Werner ◽  
Thomas Walther ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Organic Semiconductors ◽  
Fungal Growth ◽  
Nitrogen Sources ◽  
Soft Rot ◽  
Pigment Production ◽  
Primary Metabolism ◽  
Green Pigment ◽  
Organic Nitrogen Source ◽  
Complex Organic

The blue-green pigment xylindein, produced by the soft rot fungus Chlorociboria aeruginascens, is of considerable interest for various applications such as the veneer industry or organic semiconductors. The studies presented were performed in order to understand the fungal growth as well as the pigment production of C. aeruginascens. Therefore, various nutrient compositions were investigated. As a result, observations of the formation of xylindein through C. aeruginascens decoupling from growth were made. In the primary metabolism the uncolored biomass is formed. Various carbohydrates were determined as nutrients for the fungus and as a nitrogen source it was observed that the fungus prefers the complex organic nitrogen source, that being yeast extract. Furthermore, it was discovered that the ratio between carbohydrate and nitrogen sources encourages the switch of the metabolism and therewith the production of the blue-green pigment xylindein.

Evaluation of ozonation and cryptic growth for biosolids management in wastewater treatment

1999 ◽  
Vol 39 (10-11) ◽  
pp. 155-158 ◽  
Author(s):  
E. Egemen ◽  
J. Corpening ◽  
J. Padilla ◽  
R. Brennan ◽  
N. Nirmalakhandan
Keyword(s):  
Activated Sludge ◽  
Continuous Flow ◽  
Growth Conditions ◽  
Aeration Tank ◽  
Biomass Growth ◽  
Waste Sludge ◽  
Process Configuration ◽  
Activated Sludge System ◽  
Expensive Problems ◽  
Cryptic Growth

The ultimate disposal of biosolids has been and continues to be one of the most expensive problems faced by wastewater utilities. The objective of this research is to develop a process configuration for reducing the waste sludge generation in an activated sludge plant by promoting cryptic growth conditions (i.e., biomass growth on intracellular products). For this purpose, excess biosolids from a continuous flow activated sludge system were solubilized using ozone as the cell lysis agent, and then returned to the aeration tank. It is hypothesized that growth under such cryptic conditions will result in low net microbial yields, and hence, minimal net solids wastage. The results of these preliminary studies indicate that the proposed process configuration has the potential to reduce the waste sludge production by 40% to 60%.

scholarly journals Mycelial biomass cultivation of Lentinus crinitus

2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Itaruã Machri Colla ◽  
Olavo Bilac Quaresma de Oliveira Filho ◽  
Janyeli Dorini Silva de Freitas ◽  
Míria Benetati Delgado Bertéli ◽  
Giani Andrea Linde ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Nitrogen Concentration ◽  
Fungal Growth ◽  
Malt Extract ◽  
Biomass Growth ◽  
Mycelial Biomass ◽  
Protein Nitrogen ◽  
Temperature Growth ◽  
Nitrogen Concentrations ◽  
High Concentrations

Lentinus crinitus is a medicinal basidiomycete, little studied regarding the basic cultivation conditions, which is used in bioremediation and consumed by native Indians from the Brazilian Amazon. Also, it produces a fungal secondary metabolite panepoxydone that has been described as an essential regulator of the inflammatory and immune response. This study aimed to evaluate basic conditions of temperature, pH, and nitrogen concentration and source in the cultivation of L. crinitus mycelial biomass. In order to evaluate fungal growth temperature, 2% malt extract agar (MEA) medium, pH 5.5, was utilized from 19 to 40 °C. For pH, MEA had pH adjusted from 2 to 11 and cultivated at 28 °C. Urea or soybean meal was added to MEA to obtain final concentration from 0.5 and 16 g/L of nitrogen, pH of 5.5, cultivated at 28 °C. The best temperature growth varies from 31 to 34 ºC and the optimal one is 32.7º C, and the best pH ranges from 4.5 to 6.5 and the optimal one is 6.1. Protein or non-protein nitrogen concentration is inversely proportional to the mycelial biomass growth. Nitrogen concentrations of 2.0 g/L soybean meal and urea inhibit mycelial biomass growth in 11% and 12%, respectively, but high concentrations of 16.0 g/L nitrogen inhibit the growth in 46% and 95%, respectively. The fungus is robust and grows under extreme conditions of temperature and pH, but smaller adaptation with increasing nitrogen concentrations in the cultivation medium, mainly non-protein nitrogen.

Condensed conifer tannins as antifungal agents in liquid culture

Holzforschung ◽  
2013 ◽  
Vol 67 (7) ◽  
pp. 825-832 ◽  
Author(s):  
Anna-Kaisa Anttila ◽  
Anna Maria Pirttilä ◽  
Hely Häggman ◽  
Anni Harju ◽  
Martti Venäläinen ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Fungal Growth ◽  
White Rot Fungi ◽  
Brown Rot ◽  
Wood Preservative ◽  
Soft Rot ◽  
White Rot ◽  
Wood Preservatives ◽  
Active Components ◽  
Brown Rot Fungi

Abstract In the last decades, many wood preservatives have been prohibited for their ecotoxicity. The present article is focusing on the conifer-derived condensed tannins as environment-friendly options for the substitution of artificial wood preservatives. Eight different tannin fractions were extracted from spruce cones, spruce barks, and pine cones. The parameters of tannin extraction, such as the methods of purification and concentration of active components in the extracts, have been investigated. The cone and bark extracts were tested for the growth inhibition of eight brown-rot fungi, three white-rot fungi, and four soft-rot fungi in liquid cultures. The cone tannins provided a more efficient fungal growth inhibition than bark tannins. Purification increased the antifungal properties of the extracts. The growth of brown-rot fungi was inhibited by the tannins already at low concentrations. However, the extracts were not effective against the white-rot or soft-rot fungi. More investigation is needed concerning the tannin source and the purification procedure of the extracts before tannins can be considered as an ecologically benign wood preservative.

Stroma-free long-term growth of primary acute myeloid leukemia (AML) cells.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e18535-e18535
Author(s):  
Jing Chen ◽  
Glorymar Ibanez Sanchez ◽  
Paul Calder ◽  
Mark G. Frattini
Keyword(s):  
Growth Factors ◽  
Conditioned Medium ◽  
Stromal Cells ◽  
Drug Sensitivity ◽  
Growth Conditions ◽  
Leukemic Cells ◽  
Sensitivity Testing ◽  
Drug Sensitivity Testing

e18535 Background: To individualize therapy for relapsed/refractory AML patients, optimal in-vitro culture conditions to support primary leukemic cells are essential for drug sensitivity testing. Our lab has validated a high throughput chemosensitivity assay with primary AML cells maintained by growth factors (cytokines); however, growth factors have not been shown to support long-term assays of primary AML cells. Stromal cells of the tumor microenvironment are crucial to maintain normal hematopoiesis and leukemic cells and have been shown to support long term in-vitro expansion of primary AML cells. However, there is little information characterizing these growth conditions. The aim of this study was to compare long-term proliferation and phenotypes of primary AML cells with growth factors or stromal support to best determine their utility as a platform for drug sensitivity testing in functional assays. Methods: Patient-derived AML cells were cultured in 96-well plates in: 1) cell culture medium only 2) Human HS5 or HS27 stromal cells 3) HS5 or HS27 stroma-conditioned media or 4) cytokine cocktail. Viability readout by Guava ViaCount and leukemic cell surface phenotypes by fluorescently-conjugated antibodies were performed weekly over 3 weeks. Results: Primary AML cells cultured with only cytokines maintained proliferation at 3 weeks. In comparison, AML cells cultured in HS5 stroma-conditioned medium also maintained proliferation at a similar rate at 3 weeks, while co-culture with HS5 stromal cells demonstrated significantly higher proliferation. Leukemic immunophenotypes were maintained for all growth conditions over 3 weeks. Conclusions: Contrary to known data, primary AML cells with cytokines continued to expand at 3 weeks, at a similar rate to HS5 stroma-conditioned medium, a finding that has not been reported. Consistent with previous studies, we confirmed that stromal cells such as HS5 can provide long-term in-vitro expansion of primary AML cells, which cannot be substituted by stroma-conditioned medium. The ability to maintain long-term expansion of primary AML cells by both cytokines and stromal cells sets up a platform for a direct comparison of high throughput drug sensitivity testing under these growth conditions.

The ecology of fungi infecting untreated sapwood of Pinus radiata

1968 ◽  
Vol 46 (12) ◽  
pp. 1577-1589 ◽  
Author(s):  
John A. Butcher
Keyword(s):  
Moisture Content ◽  
Pinus Radiata ◽  
Fungal Growth ◽  
Soft Rot ◽  
Airborne Fungi ◽  
Effect Of Temperature ◽  
Decay Fungi ◽  
Source Of Infection ◽  
Blue Stain Fungi ◽  
Blue Stain

The succession of fungi colonizing untreated stakes of Pinus radiata sapwood was followed in aboveground, groundline, and belowground zones. The course and speed of the succession in these zones was influenced both by the source of infection and the moisture content of the wood. Aboveground moisture content of the stakes limited development, and colonization by airborne fungi (blue-stain fungi to molds) did not proceed to the stage where decay fungi became established. At groundline and below where the succession of organisms (primary molds to soft-rot fungi to secondary molds and basidiomycetes) was complete, moisture content was more favorable for fungal growth. The succession proceeded more slowly belowground than at groundline because of (1) higher moisture content of the wood and (2) colonization being restricted to soil-borne fungi. Relative frequencies of fungi in each zone are tabulated and discussed. Decay capability tests were made to aid in establishing the role of soft rot and basidiomycetous fungi. The basidiomycetes encountered were not those usually isolated from posts of P. radiata in an advanced state of decay. Comparative studies of the effect of temperature and pH on the growth rate of the various fungi isolated were of little value in explaining the broad bases for the successional trends. Some basic problems remaining in ecological studies of wood-inhabiting fungi are discussed.

scholarly journals Pumpkin Fruit Rot in North Carolina Caused by Phytophthora nicotianae

Plant Disease ◽  
2000 ◽  
Vol 84 (8) ◽  
pp. 923-923
Author(s):  
G. J. Holmes
Keyword(s):  
Fungal Growth ◽  
Average Diameter ◽  
Soft Rot ◽  
Selective Medium ◽  
Phytophthora Nicotianae ◽  
Crown Rot ◽  
Fruit Rot ◽  
Unknown Etiology ◽  
Postharvest Diseases ◽  
Phytophthora Spp

In 1999, during an evaluation of pumpkin (Cucurbita pepo) fruit for susceptibility to naturally occurring postharvest diseases, a soft rot of unknown etiology was noted. No fungal growth or sporulation was seen on the fruit surface and no root or crown rot was observed in the field. When fruit were cross-sectioned, masses of white, floccose mycelium covering large sections of the seed cavity were observed. Rot was observed in 21 fruit (6.4% of the total). The fungus was isolated from symptomatic fruit on a modified P10ARPH agar medium, semi-selective for Phytophthora spp. (2). Isolates from eight fruit formed papillate, ovoid sporangia, abundant chlamydospores, and colonies characteristic of P. nicotianae (1). No oospores were produced. Four sound pumpkin fruit (cv. Early Autumn) were inoculated with four isolates (one isolate per fruit). Each isolate was recovered from a different fruit. Pumpkins were surface sterilized at the point of inoculation by wetting with 70% ethanol. Inoculation was done by removing a small amount of mycelium from pure culture using a sterile, wooden toothpick and inserting it 2 cm deep into opposite sides of the mid section of sound fruit (two inoculations per fruit). Control fruit were punctured with sterile toothpicks (once per fruit). First symptoms appeared 4 days after inoculation at room temperature (22 to 24°C). Symptoms consisted of circular, water-soaked areas originating from the point of inoculation. Average diameter (based on four measurements on two fruit) of the water-soaked lesions were 3 cm at first appearance (i.e., 4 days) and 11 cm 10 days after inoculation. No symptoms developed on controls. When symptomatic fruit were cross-sectioned, masses of white, floccose mycelium were noted. Reisolation of this mycelium onto selective medium yielded P. nicotianae, thus fulfilling Koch's postulates. This is the first report of P. nicotianae causing fruit rot of pumpkins. References: (1) D. C. Erwin and O. K. Ribeiro. 1996. Phytophthora Diseases Worldwide. The American Phytopathological Society, St. Paul, MN. (2) H. D. Shew. Phytopathology 77:1090, 1987.

scholarly journals Growth Promoting and Stabilizing of Cucumber Plants Cultivated in Soilless Cultivation Systems Using Biostimulators

2021 ◽  
Author(s):  
Michael Henry Böhme
Keyword(s):  
Temperature Stress ◽  
Ph Value ◽  
Growth Conditions ◽  
Cucumber Plant ◽  
Growth Promoters ◽  
Cultivation Systems ◽  
Stress Prevention ◽  
Soilless Cultivation ◽  
Growth Promoting ◽  
Short Time

Cultivation of cucumber in greenhouses is predominantly carried out in soilless cultivation systems mainly in substrate culture. The use of organic and completely compostable substrates is of rising interest in such systems, unclean sheep wool was formed as substrate slabs, peat slabs and coconut fiber slabs were compared with mineral substrates rockwool and perlite. In general can be stated, intensively in greenhouses cultivated crops such as cucumbers, suffer often from inadequate abiotic or biotic growth conditions in particular in the rhizosphere. Many studies were done to find growth promoters or biostimulators to stabilize the growing condition in the rhizosphere, in case of stress situation as fluctuating salt concentration EC and pH value, but also in case of temperature stress. K-Humate, Lactate and Bacillus subtilis were investigated as biostimulators in such situations. Different concentration and combination of these biostimulators were investigated but also the methods of application on leaves and roots respectively. Very successful was used for the stabilization of the EC value for cucumber plants growing in substrate the application of K-Humate and B. subtilis (FZB24®) as single component and combined. Following the results, it can be assumed, the application of the combined biostimulators with all substances if applied over the roots was a stimulating effect visible, whereas if applied over the leaves an inhibiting effect for cucumber plant growth. Application of the biostimulators solution with all three components BS-FZB24® (0.2%), K-Humate (0.01%), and LACTOFOL “O” (0.1%) were tested regarding their effects in case of strong but short time pH and temperature stress the growth of cucumber plants. There are a strong correlation between green biomass of treated cucumber plants and their root mass. It can be assumed that one of the effects of stress prevention through the biostimulators is primarily based on increasing root growth.

scholarly journals Effect of Ambient pH on Growth, Pathogenicity, and Patulin Production of Penicillium expansum

Toxins ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 550
Author(s):  
Carelle Kouasseu Jimdjio ◽  
Huali Xue ◽  
Yang Bi ◽  
Mina Nan ◽  
Lan Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factors ◽  
Soft Rot ◽  
Penicillium Expansum ◽  
Expression Of Genes ◽  
Transmission Electron ◽  
Expression Studies ◽  
Gene Expression Studies

Penicillium expansum is an important postharvest pathogen of pomaceous fruit and a causal agent of blue mold or soft rot. In this study, we investigated the effect of ambient pH on growth, ultrastructure alteration, and pathogenicity of P. expansum, as well as accumulation of patulin and expression of genes involved in patulin biosynthesis. Under different pH, the fungus was routinely cultured and collected for growth, pathogenicity, patulin production, and gene expression studies using transmission electron microscopy, apple inoculation, HPLC, and RT-qPCR methods. Different ambient pH had significant impact on expression of genes and growth factors involved in patulin biosynthesis. Under same range of pH, gene expression profile, growth factors, and patulin accumulation (in vivo and in vitro) all showed similar changing trends. A well-developed cell was observed in addition to upregulation of genes at pH between pH 5.0 and 7.0, while the opposite was observed when pH was too basic (8.5) or too acid (2.5). Additionally, ambient pH had direct or indirect influence on expression of PecreaA, PelaeA, and PepacC. These findings will help in understanding the effect of ambient pH on growth, pathogenicity, and patulin production and support the development of successful methods for combating P. expansum infection on apple fruits.

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