scholarly journals Caenorhabditis elegans as a Model Host to Monitor the Candida Infection Processes

2018 ◽  
Vol 4 (4) ◽  
pp. 123 ◽  
Author(s):  
Asmaa Elkabti ◽  
Luca Issi ◽  
Reeta Rao
Keyword(s):  
Low Cost ◽  
Petri Dish ◽  
Loss Of Function ◽  
Genetic Stock ◽  
C Elegans ◽  
Long Term Storage ◽  
Large Brood ◽  
Opportunistic Fungal Pathogen ◽  
Infection Processes

C. elegans has several advantages as an experimental host for the study of infectious diseases. Worms are easily maintained and propagated on bacterial lawns. The worms can be frozen for long term storage and still maintain viability years later. Their short generation time and large brood size of thousands of worms grown on a single petri dish, makes it relatively easy to maintain at a low cost. The typical wild type adult worm grows to approximately 1.5 mm in length and are transparent, allowing for the identification of several internal organs using an affordable dissecting microscope. A large collection of loss of function mutant strains are readily available from the C. elegans genetic stock center, making targeted genetic studies in the nematode possible. Here we describe ways in which this facile model host has been used to study Candida albicans, an opportunistic fungal pathogen that poses a serious public health threat.

Susceptibility of Japanese pears to low concentrations of ethylene during storage

2007 ◽  
Vol 47 (12) ◽  
pp. 1480 ◽  
Author(s):  
M. J. Szczerbanik ◽  
K. J. Scott ◽  
J. E. Paton ◽  
D. J. Best
Keyword(s):  
Carbon Dioxide ◽  
Low Cost ◽  
Pyrus Pyrifolia ◽  
Green Colour ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Internal Browning ◽  
Term Storage

The ‘Nijisseiki’ cultivar of Japanese pears (Pyrus pyrifolia) is also known as nashi in Australia. Nashi were exposed to levels of <0.005, 0.01, 0.1 and 1.0 µL/L of ethylene in air during 26 weeks storage at 0°C. Levels of ethylene as low as 0.01 µL/L increased chlorophyll loss and visual green colour. Increasing ethylene levels also increased softening and internal browning, although flesh spot decay was reduced in the presence of ethylene. While it would be worthwhile to remove ethylene during long-term storage of ‘Nijisseiki’ in air, another alternative, adding 2% carbon dioxide to the atmosphere, is suggested as a possible low cost means to overcome the ripening effect of ethylene.

scholarly journals Capture and Purification of Plant Genomic DNA on a Readily-available Cellulose Matrix

2017 ◽  
Author(s):  
Megan M. Thompson ◽  
Estelle M. Hrabak
Keyword(s):  
Arabidopsis Thaliana ◽  
Nucleic Acids ◽  
Plant Species ◽  
Efficient Method ◽  
Genomic Dna ◽  
Low Cost ◽  
Long Term Storage ◽  
Cellulose Matrix ◽  
Term Storage

AbstractWhatman FTA ®Cards are a fast and efficient method for capturing and storing nucleic acids but are cost-prohibitive for some researchers. We developed a method that substitutes readily-available cellulose-based paper and homemade washing buffer for commercial FTA ®Cards and FTA ®Purification Reagent. This method is suitable for long-term storage of DNA from many plant species, including Arabidopsis thaliana, prior to purification and PCR.Method SummaryHere we report a low-cost method for long-term storage of plant genomic DNA on a readily available cellulose matrix.

Evaluation of a low-cost procedure for sampling, long-term storage, and extraction of RNA from blood for qPCR analyses

2015 ◽  
Vol 53 (8) ◽  
Author(s):  
Rasmus B. Mærkedahl ◽  
Hanne Frøkiær ◽  
Lotte Lauritzen ◽  
Stine B. Metzdorff
Keyword(s):  
Gene Expression ◽  
Clinical Trials ◽  
Low Cost ◽  
Rna Extraction ◽  
Initial Step ◽  
Rna Integrity ◽  
Long Term Storage ◽  
Significant Cost Reduction ◽  
Term Storage

AbstractIn large clinical trials, where RNA cannot be extracted immediately after sampling, preserving RNA in whole blood is a crucial initial step in obtaining robust qPCR data. The current golden standard for RNA preservation is costly and designed for time-consuming column-based RNA-extraction. We investigated the use of lysis buffer for long-term storage of blood samples for qPCR analysis.Blood was collected from 13 healthy adults and diluted in MagMAX lysis/binding solution or PAXgene Blood RNA tubes and stored at –20 °C for 0, 1, or 4 months before RNA extraction by the matching method. RNA integrity, yield and purity were evaluated and the methods were compared by subsequent analyses of the gene expression levels ofThe MagMAX system extracted 2.3–2.8 times more RNA per mL blood, with better performance in terms of purity, and with comparable levels of integrity relative to the PAXgene system. Gene expression analysis using qPCR of: The MagMAX system can be used for storage of human blood for up to 4 months and is equivalent to the PAXgene system for RNA extraction. It furthermore, provides a means for significant cost reduction in large clinical trials.

scholarly journals Long-Term Storage of Small Natural History Specimens Using Gelatin Capsules: A Case Study from the Royal Alberta Museum

2018 ◽  
Vol 32 (1) ◽  
pp. 31-46
Author(s):  
Diana Tirlea ◽  
Carmen Li ◽  
Alwynne B. Beaudoin ◽  
Emily Moffat
Keyword(s):  
Continuous Monitoring ◽  
Low Cost ◽  
Ease Of Use ◽  
Gelatin Capsules ◽  
Long Term Storage ◽  
Pros And Cons ◽  
Storage Methods ◽  
Term Storage

Abstract Museums use gelatin capsules to store small objects and specimens, despite limited documentation of their long-term viability. The Royal Alberta Museum (RAM of Canada) uses gelatin capsules to store seeds, bones, and plant material because of their ease of use, transparency, soft-bodied walls, size availability, and low cost. Recently, RAM staff reported damaged capsules from the palaeontology collections. We evaluated 499 capsules used to store specimens accessioned in 1986 and 1988 and investigated capsule properties using Fourier transform infrared spectroscopy and Oddy testing. Only 4.21% of inspected capsules were dented, cracked, and/or fractured. Based on interviews and testing, we determined that damage to capsules likely resulted during handling (i.e., applied force when opening). We conclude that gelatin capsules offer a good, inexpensive method for long-term storage of small, dried specimens in environmentally controlled conditions. Alternatives to gelatin capsules exist, although their pros and cons require evaluation before use. All storage methods require continuous monitoring for signs of container or specimen deterioration.

Low-Cost, Low-Tech Biosolids Treatment via Long-Term Storage: A Comparison of Two Pilot-Scale Studies

2018 ◽  
Vol 2018 (4) ◽  
pp. 1143-1150
Author(s):  
Jennifer G Becker ◽  
Karina Eyre ◽  
Tanner Keyzers ◽  
Christa Meingast ◽  
Eric A Seagren
Keyword(s):  
Low Cost ◽  
Pilot Scale ◽  
Long Term Storage ◽  
Term Storage

scholarly journals BIOLOGY OF SEED GERMINATION IN SOME ONION SPECIES (ALLIUM L.)

2021 ◽  
Vol 3 (27) ◽  
pp. 180-190
Author(s):  
T.I. Fomina ◽  
Keyword(s):  
Reproductive Potential ◽  
Petri Dish ◽  
Botanical Garden ◽  
Type I ◽  
Type Ii ◽  
Long Term Storage ◽  
Germinating Seeds ◽  
Term Storage ◽  
Onion Species

The possibility of seed reproduction is one of the factors of successful cultivation of resource plants in specific environmental conditions. The aim of the work was to study the germination behavior, quality and longevity of seeds in 15 onions (Allium L.) from the collection of the Central Siberian Botanical Garden SB RAS, Novosibirsk. The research was carried out in 1996–2019. Laboratory germination of seeds was determined according to generally accepted methods. Seeds were tested 3–7 months after harvesting at 17–23 °C in the light. The number of seeds in each Petri dish – 25–50; double replication. In the case of hindered germination, a two-month chilling at 4 °C was applied. In the future, laboratory germination was determined after 3, 5 and 7 years of room storage. We have established that onion seeds have three types of germinating. Seeds of type I – eight species, one variety from the subgenus Rhizirideum – usually lack dormancy. Germination is fast and simultaneous, and average germination percentages are 77.1–92.2 %. Seeds of type II – A. leucocephalum and A. microdictyon from the subgenus Rhizirideum, also A. caeruleum and A. flavum from the subgenus Allium – are characterized by stretched germination period due to the shallow dormancy, and their germination percentages vary from 32.6 % to 81.1 %. Seeds of type III – A. obliquum from the subgenus Rhizirideum and two species of the subgenus Melanocrommyum – do not germinate in room conditions, or germinate with low germination percentages, whereas chilling increases them up to 47.0–67.0 %. The hindered germination of these seeds is due to the deep organic dormancy. The economic longevity of onion seeds is 3–5 years, and the biological longevity varies at interspecific level within 5–8 years. Thus, the species that produce plump and good germinating seeds suitable for long-term storage – A. altaicum, A. bidentatum, A. flavum, A. microdictyon, A. nutans, A. ramosum, A. rubens, A. schoenoprasum, A. senescens, A. senescens var. glaucum, and A. strictum – have the highest reproductive potential in culture.

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