scholarly journals Capture and Purification of Plant Genomic DNA on a Readily-available Cellulose Matrix

2017 ◽  
Author(s):  
Megan M. Thompson ◽  
Estelle M. Hrabak
Keyword(s):  
Arabidopsis Thaliana ◽  
Nucleic Acids ◽  
Plant Species ◽  
Efficient Method ◽  
Genomic Dna ◽  
Low Cost ◽  
Long Term Storage ◽  
Cellulose Matrix ◽  
Term Storage

AbstractWhatman FTA ®Cards are a fast and efficient method for capturing and storing nucleic acids but are cost-prohibitive for some researchers. We developed a method that substitutes readily-available cellulose-based paper and homemade washing buffer for commercial FTA ®Cards and FTA ®Purification Reagent. This method is suitable for long-term storage of DNA from many plant species, including Arabidopsis thaliana, prior to purification and PCR.Method SummaryHere we report a low-cost method for long-term storage of plant genomic DNA on a readily available cellulose matrix.

Susceptibility of Japanese pears to low concentrations of ethylene during storage

2007 ◽  
Vol 47 (12) ◽  
pp. 1480 ◽  
Author(s):  
M. J. Szczerbanik ◽  
K. J. Scott ◽  
J. E. Paton ◽  
D. J. Best
Keyword(s):  
Carbon Dioxide ◽  
Low Cost ◽  
Pyrus Pyrifolia ◽  
Green Colour ◽  
Long Term Storage ◽  
Low Concentrations ◽  
Internal Browning ◽  
Term Storage

The ‘Nijisseiki’ cultivar of Japanese pears (Pyrus pyrifolia) is also known as nashi in Australia. Nashi were exposed to levels of <0.005, 0.01, 0.1 and 1.0 µL/L of ethylene in air during 26 weeks storage at 0°C. Levels of ethylene as low as 0.01 µL/L increased chlorophyll loss and visual green colour. Increasing ethylene levels also increased softening and internal browning, although flesh spot decay was reduced in the presence of ethylene. While it would be worthwhile to remove ethylene during long-term storage of ‘Nijisseiki’ in air, another alternative, adding 2% carbon dioxide to the atmosphere, is suggested as a possible low cost means to overcome the ripening effect of ethylene.

Evaluation of a low-cost procedure for sampling, long-term storage, and extraction of RNA from blood for qPCR analyses

2015 ◽  
Vol 53 (8) ◽  
Author(s):  
Rasmus B. Mærkedahl ◽  
Hanne Frøkiær ◽  
Lotte Lauritzen ◽  
Stine B. Metzdorff
Keyword(s):  
Gene Expression ◽  
Clinical Trials ◽  
Low Cost ◽  
Rna Extraction ◽  
Initial Step ◽  
Rna Integrity ◽  
Long Term Storage ◽  
Significant Cost Reduction ◽  
Term Storage

AbstractIn large clinical trials, where RNA cannot be extracted immediately after sampling, preserving RNA in whole blood is a crucial initial step in obtaining robust qPCR data. The current golden standard for RNA preservation is costly and designed for time-consuming column-based RNA-extraction. We investigated the use of lysis buffer for long-term storage of blood samples for qPCR analysis.Blood was collected from 13 healthy adults and diluted in MagMAX lysis/binding solution or PAXgene Blood RNA tubes and stored at –20 °C for 0, 1, or 4 months before RNA extraction by the matching method. RNA integrity, yield and purity were evaluated and the methods were compared by subsequent analyses of the gene expression levels ofThe MagMAX system extracted 2.3–2.8 times more RNA per mL blood, with better performance in terms of purity, and with comparable levels of integrity relative to the PAXgene system. Gene expression analysis using qPCR of: The MagMAX system can be used for storage of human blood for up to 4 months and is equivalent to the PAXgene system for RNA extraction. It furthermore, provides a means for significant cost reduction in large clinical trials.

scholarly journals Studies on genetic changes in rye samples (Secale cereale L.) maintained in a seed bank

2006 ◽  
Vol 11 (3) ◽  
Author(s):  
Katarzyna Chwedorzewska ◽  
Piotr Bednarek ◽  
Renata Lewandowska ◽  
Paweł Krajewski ◽  
Jerzy Puchalski
Keyword(s):  
Genomic Dna ◽  
Fragment Length Polymorphism ◽  
Gene Bank ◽  
Genetic Changes ◽  
Long Term Storage ◽  
Seed Ageing ◽  
Secale Cereale L ◽  
Principle Coordinate Analysis ◽  
Term Storage

AbstractThe aim of this study was to identify genetic changes in rye seeds induced by natural ageing during long-term storage and consecutive regeneration cycles under gene bank conditions. Genomic DNA from four rye samples varying in their initial viability after one and three cycles of reproduction was analyzed by AFLP (amplified fragment length polymorphism) fingerprinting. Seven EcoRI/MseI primer combinations defined 663 fragments, and seven PstI/MseI primer combinations defined 551 fragments. The variation in the frequency of the seventy-four EcoRI/MseI bands was statistically significant between samples. These changes could be attributed to genetic changes occurring during storage and regeneration. However, the PstI/MseI fragments appeared to be uninfluenced by seed ageing, regeneration and propagation. A combined Principle Coordinate Analysis revealed differences between samples with different initial viability. We showed that materials with low initial viability differ in their response from highly viable ones, and that the changes exhibited in the former case are preserved through regeneration cycles.

scholarly journals Stability of Hepatitis C Virus, HIV, and Hepatitis B Virus Nucleic Acids in Plasma Samples after Long-Term Storage at −20oC and −70oC

2011 ◽  
Vol 49 (9) ◽  
pp. 3163-3167 ◽  
Author(s):  
Cristina Baleriola ◽  
Harpreet Johal ◽  
Brendan Jacka ◽  
Sandra Chaverot ◽  
Scott Bowden ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Nucleic Acids ◽  
Plasma Samples ◽  
Long Term Storage ◽  
B Virus ◽  
Term Storage

scholarly journals Long-Term Storage of Small Natural History Specimens Using Gelatin Capsules: A Case Study from the Royal Alberta Museum

2018 ◽  
Vol 32 (1) ◽  
pp. 31-46
Author(s):  
Diana Tirlea ◽  
Carmen Li ◽  
Alwynne B. Beaudoin ◽  
Emily Moffat
Keyword(s):  
Continuous Monitoring ◽  
Low Cost ◽  
Ease Of Use ◽  
Gelatin Capsules ◽  
Long Term Storage ◽  
Pros And Cons ◽  
Storage Methods ◽  
Term Storage

Abstract Museums use gelatin capsules to store small objects and specimens, despite limited documentation of their long-term viability. The Royal Alberta Museum (RAM of Canada) uses gelatin capsules to store seeds, bones, and plant material because of their ease of use, transparency, soft-bodied walls, size availability, and low cost. Recently, RAM staff reported damaged capsules from the palaeontology collections. We evaluated 499 capsules used to store specimens accessioned in 1986 and 1988 and investigated capsule properties using Fourier transform infrared spectroscopy and Oddy testing. Only 4.21% of inspected capsules were dented, cracked, and/or fractured. Based on interviews and testing, we determined that damage to capsules likely resulted during handling (i.e., applied force when opening). We conclude that gelatin capsules offer a good, inexpensive method for long-term storage of small, dried specimens in environmentally controlled conditions. Alternatives to gelatin capsules exist, although their pros and cons require evaluation before use. All storage methods require continuous monitoring for signs of container or specimen deterioration.

scholarly journals A feasible method to extract DNA from the cambium of high-canopy trees: from harvest to assessment

2020 ◽  
Vol 50 (4) ◽  
pp. 335-338
Author(s):  
Érica MANGARAVITE ◽  
Vanessa TERRA ◽  
Eric Koiti Okiyama HATTORI ◽  
Thaís Carolina da Silva DAL’SASSO ◽  
Leonardo Lopes BHERING ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Storage Conditions ◽  
Microsatellite Genotyping ◽  
Short Term ◽  
Feasible Method ◽  
Long Term Storage ◽  
Canopy Trees ◽  
Amazonian Forests ◽  
Term Storage

ABSTRACT Many tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies. We adapted a feasible methodology for extracting genomic DNA from cambium tissue harvested in the field for the assessment with PCR. We tested three storage conditions (two buffers and a silica gel) and four periods of time after harvest. We used previously described protocols and tested them on three species that occur in Amazonian forests and other biomes: Anadenanthera peregrina var. peregrina, Cedrela fissilis, and Ceiba speciosa. Our protocol obtained suitable PCR-grade genomic DNA for DNA sequencing and microsatellite genotyping. We recommend the use of silica for long-term storage and the buffer with ascorbic acid for short-term storage.

Low-Cost, Low-Tech Biosolids Treatment via Long-Term Storage: A Comparison of Two Pilot-Scale Studies

2018 ◽  
Vol 2018 (4) ◽  
pp. 1143-1150
Author(s):  
Jennifer G Becker ◽  
Karina Eyre ◽  
Tanner Keyzers ◽  
Christa Meingast ◽  
Eric A Seagren
Keyword(s):  
Low Cost ◽  
Pilot Scale ◽  
Long Term Storage ◽  
Term Storage

scholarly journals FTA Cards for Preservation of Nucleic Acids for Molecular Assays: A Review on the Use of Cytologic/Tissue Samples

2018 ◽  
Vol 142 (3) ◽  
pp. 308-312 ◽  
Author(s):  
Gilda da Cunha Santos
Keyword(s):  
Nucleic Acids ◽  
Molecular Techniques ◽  
Long Distance ◽  
High Quality ◽  
Molecular Assays ◽  
Fta Cards ◽  
Long Term Storage ◽  
Term Storage

Context.— Traditional methods for storing histologic and cytologic specimens for future use in molecular assays have consisted of either snap-freezing with cryopreservation or formalin-fixing, paraffin-embedding the samples. Although snap-freezing with cryopreservation is recommended for better preservation of nucleic acids, the infrastructure and space required for archiving impose challenges for high-volume pathology laboratories. Cost-effective, long-term storage at room temperature; relatively easy shipment; and standardized handling can be achieved with formalin-fixed, paraffin-embedded samples, but formalin fixation induces fragmentation and chemical modification of nucleic acids. Advances in next-generation sequencing platforms, coupled with an increase in diagnostic, prognostic, and predictive molecular biomarkers have created a demand for high-quality nucleic acids. To address issues of the quality of nucleic acid and logistics in sample acquisition, alternatives for specimen preservation and long-term storage have been described and include novel universal tissue fixatives, stabilizers, and technologies. Objective.— To collect, retrieve, and review information from studies describing the use of nucleic acids recovered from cytologic/tissue specimens stored on Flinders Technology Associates (FTA, GE Whatman, Maidstone, Kent, United Kingdom) cards for downstream molecular applications. Data Sources.— An electronic literature search in the PubMed (National Center for Biotechnology Information, Bethesda, Maryland) database allowed the selection of manuscripts addressing the use of FTA cards for storage of cytologic samples for molecular analysis. Only articles published in English were retrieved. Conclusions.— The use of FTA cards is a versatile method for fostering multicenter, international collaborations and clinical trials that require centralized testing, long-distance shipment, and high-quality nucleic acids for molecular techniques. Studies with controlled temperature are required to test the quality of recovered RNA after long-term storage.

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