scholarly journals Superficial Characteristics of Titanium after Treatment of Chorreated Surface, Passive Acid, and Decontamination with Argon Plasma

2018 ◽  
Vol 9 (4) ◽  
pp. 71 ◽  
Author(s):  
María Rizo-Gorrita ◽  
Irene Luna-Oliva ◽  
María-Angeles Serrera-Figallo ◽  
Daniel Torres-Lagares
Keyword(s):  
Dental Implants ◽  
Photoelectron Spectroscopy ◽  
Bone Growth ◽  
Relative Concentration ◽  
Argon Plasma ◽  
Titanium Implants ◽  
Total Carbon ◽  
Coarse Grained ◽  
Topographic Analysis ◽  
Total Carbon Content

(1) Background. Titanium is characterized by its biocompatibility, resistance to maximum stress, and fatigue and non-toxicity. The composition, surface structure, and roughness of titanium have a key and direct influence on the osseointegration processes when it is used in the form of dental implants. The objective of the present study is to characterize, at chemical, superficial, and biological levels, the result of the application of the sandblasted with large-grit and acid-etched (SLA) treatment consisting of coarse-grained and double-passivated acid blasting with subsequent decontamination with argon plasma on the surface of titanium implants type IV. (2) Methods. Four Oxtein® dental implants (Zaragoza, Spain) were investigated with the following coding: Code L63713T (titanium grade IV, 3.75 mm in diameter, and 13 mm in length). The surface of the implants was SLA type obtained from coarse-grained, double passivated acid, and decontaminated with argon plasma. The samples were in their sealed packages and were opened in our laboratory. The X-ray photoelectron spectroscopy (XPS) technique was used to characterize the chemical composition of the surface, and the scanning electronic microscope (SEM) technique was used to perform topographic surface evaluation. Cell cultures were also performed on both surfaces. (3) Results. The superficial chemical analysis of the studied samples presented the following components, approximately, expressed in atomic percentage: O: 39%; Ti: 18%; C: 39%; N: 2%; and Si: 1%. In the same way, the topographic analysis values were obtained in the evaluated roughness parameters: Ra: 1.5 μm ± 0.02%; Rq: 1.31 μm ± 0.33; Rz: 8.98 μm ± 0.73; Rp: 5.12 μm ± 0.48; Rv: 3.76 μm ± 0.51; and Rc: 4.92 μm ± 0.24. At a biological level, the expression of osteocalcin was higher (p < 0.05) on the micro-rough surface compared to that machined at 48 and 96 h of culture. (4) Conclusions. The data obtained in our study indicate that the total carbon content, the relative concentration of titanium, and the roughness of the treatment performed on the implants are in agreement with those found in the literature. Further, the roughness of the treatment performed on the implants throws a spongy, three-dimensional surface suitable for bone growth on it. The biological results found are compatible with the clinical use of the surface tested.

scholarly journals Influence of Titanium Oxide Pillar Array Nanometric Structures and Ultraviolet Irradiation on the Properties of the Surface of Dental Implants—A Pilot Study

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1458 ◽  
Author(s):  
Leon-Ramos ◽  
Diosdado-Cano ◽  
López-Santos ◽  
Barranco ◽  
Torres-Lagares ◽  
...  
Keyword(s):  
Contact Angle ◽  
Cell Growth ◽  
Dental Implants ◽  
Titanium Oxide ◽  
Ultraviolet Irradiation ◽  
Photoelectron Spectroscopy ◽  
Titanium Implants ◽  
Microwave Source ◽  
Pillar Array ◽  
X Ray

Aim: Titanium implants are commonly used as replacement therapy for lost teeth and much current research is focusing on the improvement of the chemical and physical properties of their surfaces in order to improve the osseointegration process. TiO2, when it is deposited in the form of pillar array nanometric structures, has photocatalytic properties and wet surface control, which, together with UV irradiation, provide it with superhydrophilic surfaces, which may be of interest for improving cell adhesion on the peri-implant surface. In this article, we address the influence of this type of surface treatment on type IV and type V titanium discs on their surface energy and cell growth on them. Materials and methods: Samples from titanium rods used for making dental implants were used. There were two types of samples: grade IV and grade V. In turn, within each grade, two types of samples were differentiated: untreated and treated with sand blasting and subjected to double acid etching. Synthesis of the film consisting of titanium oxide pillar array structures was carried out using plasma-enhanced chemical vapor deposition equipment. The plasma was generated in a quartz vessel by an external SLAN-1 microwave source with a frequency of 2.45 GHz. Five specimens from each group were used (40 discs in total). On the surfaces to be studied, the following determinations were carried out: (a) X-ray photoelectron spectroscopy, (b) scanning electron microscopy, (c) energy dispersive X-ray spectroscopy, (d) profilometry, (e) contact angle measurement or surface wettability, (f) progression of contact angle on applying ultraviolet irradiation, and (g) a biocompatibility test and cytotoxicity with cell cultures. Results: The application of ultraviolet light decreased the hydrophobicity of all the surfaces studied, although it did so to a greater extent on the surfaces with the studied modification applied, this being more evident in samples manufactured in grade V titanium. In samples made in grade IV titanium, this difference was less evident, and even in the sample manufactured with grade IV and SLA treatment, the application of the nanometric modification of the surface made the surface optically less active. Regarding cell growth, all the surfaces studied, grouped in relation to the presence or not of the nanometric treatment, showed similar growth. Conclusions. Treatment of titanium oxide surfaces with ultraviolet irradiation made them change temporarily into superhydrophilic ones, which confirms that their biocompatibility could be improved in this way, or at least be maintained.

scholarly journals Surface Activation of Titanium Dental Implants by Using UVC-LED Irradiation

2021 ◽  
Vol 22 (5) ◽  
pp. 2597
Author(s):  
Nagore Arroyo-Lamas ◽  
Iciar Arteagoitia ◽  
Unai Ugalde
Keyword(s):  
Dental Implants ◽  
Photoelectron Spectroscopy ◽  
Organic Contaminants ◽  
Light Emitting Diode ◽  
In Vitro Study ◽  
Sem Analysis ◽  
Titanium Implants ◽  
Chemical Compositions ◽  
Titanium Dental Implants

Organic contaminants significantly limit the bioactivity of titanium implants, resulting in the degradation known as the ageing of titanium. To reactivate the surfaces, they can be photofunctionalized, i.e., irradiated with C-range ultraviolet (UVC) light. This descriptive in vitro study compares the effectiveness of novel light-emitting diode (LED) technology to remove contaminant hydrocarbons from three different commercially available titanium dental implants: THD, TiUnite, and SLA. The surface topography and morphology were characterized by scanning electron microscopy (SEM). The chemical compositions were analyzed by X-ray photoelectron spectroscopy (XPS), before and after the lighting treatment, by a pair of closely placed UVC (λ = 278 nm) and LED devices for 24 h. SEM analysis showed morphological differences at the macro- and micro-scopic level. XPS analysis showed a remarkable reduction in the carbon contents after the UVC treatment: from 25.6 to 19.5 C at. % (carbon atomic concentration) in the THD; from 30.2 to 20.2 C at. % in the TiUnite; from 26.1 to 19.2 C at. % in the SLA surface. Simultaneously, the concentration of oxygen and titanium increased. Therefore, LED-based UVC irradiation decontaminated titanium surfaces and improved the chemical features of them, regardless of the kind of surface.

scholarly journals Evaluation of Peri-Implant Bone Grafting Around Surface-Porous Dental Implants: An In Vivo Study in a Goat Model

Materials ◽  
2019 ◽  
Vol 12 (21) ◽  
pp. 3606 ◽  
Author(s):  
Fahad Alshehri ◽  
Mohammed Alshehri ◽  
Terrence Sumague ◽  
Abdurahman Niazy ◽  
John Jansen ◽  
...  
Keyword(s):  
Dental Implants ◽  
Bone Grafting ◽  
Bone Growth ◽  
Bone Ingrowth ◽  
Titanium Implant ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Biomechanical Assessment ◽  
Growth Area

Dental implants with surface-porous designs have been recently developed. Clinically, peri-implant bone grafting is expected to promote early osseointegration and bone ingrowth when applied with surface-porous dental implants in challenging conditions. The aim of this study was to comparatively analyze peri-implant bone healing around solid implants and surface-porous implants with and without peri-implant bone grafting, using biomechanical and histomorphometrical assessment in a goat iliac bone model. A total of 36 implants (4.1 mm wide, 11.5 mm long) divided into three groups, solid titanium implant (STI; n = 12), porous titanium implants (PTI; n = 12) and PTI with peri-implant bone grafting using biphasic calcium phosphate granules (PTI + BCP; n = 12), were placed bilaterally in the iliac crests of six goats. The goats were sacrificed seven weeks post-operatively and then subjected to biomechanical (n = 6 per group) and histomorphometrical (n = 6 per group) assessment. The biomechanical assessment revealed no significant differences between the three types of implants. Although the peri-implant bone-area (PIBA%) measured by histomorphometry (STI: 8.63 ± 3.93%, PTI: 9.89 ± 3.69%, PTI + BCP: 9.28 ± 2.61%) was similar for the three experimental groups, the percentage of new bone growth area (BGA%) inside the porous implant portion was significantly higher (p < 0.05) in the PTI group (10.67 ± 4.61%) compared to the PTI + BCP group (6.50 ± 6.53%). These data demonstrate that peri-implant bone grafting around surface-porous dental implants does not significantly accelerate early osseointegration and bone ingrowth.

scholarly journals Macrophagic Inflammatory Response Next to Dental Implants with Different Macro- and Micro-Structure: An In Vitro Study

2021 ◽  
Vol 11 (12) ◽  
pp. 5324
Author(s):  
Maria Menini ◽  
Francesca Delucchi ◽  
Domenico Baldi ◽  
Francesco Pera ◽  
Francesco Bagnasco ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Dental Implants ◽  
In Vitro Study ◽  
Titanium Implants ◽  
Implant Surface ◽  
Bone Implant ◽  
Optimal Outcomes ◽  
Negative Controls ◽  
Inflammatory Effect

(1) Background: Intrinsic characteristics of the implant surface and the possible presence of endotoxins may affect the bone–implant interface and cause an inflammatory response. This study aims to evaluate the possible inflammatory response induced in vitro in macrophages in contact with five different commercially available dental implants. (2) Methods: one zirconia implant NobelPearl® (Nobel Biocare) and four titanium implants, Syra® (Sweden & Martina), Prama® (Sweden & Martina), 3iT3® (Biomet 3i) and Shard® (Mech & Human), were evaluated. After 4 h of contact of murine macrophage cells J774a.1 with the implants, the total RNA was extracted, transcribed to cDNA and the gene expression of the macrophages was evaluated by quantitative PCR (qPCR) in relation to the following genes: GAPDH, YWHAZ, IL1β, IL6, TNFα, NOS2, MMP-9, MMP-8 and TIMP3. The results were statistically analyzed and compared with negative controls. (3) Results: No implant triggered a significant inflammatory response in macrophages, although 3iT3 exhibited a slight pro-inflammatory effect compared to other samples. (4) Conclusions: All the samples showed optimal outcomes without any inflammatory stimulus on the examined macrophagic cells.

scholarly journals The Bone Buttress Theory: The Effect of the Mechanical Loading of Bone on the Osseointegration of Dental Implants

Biology ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 12
Author(s):  
David Chavarri-Prado ◽  
Aritza Brizuela-Velasco ◽  
Ángel Álvarez-Arenal ◽  
Markel Dieguez-Pereira ◽  
Esteban Pérez-Pevida ◽  
...  
Keyword(s):  
Dental Implants ◽  
Mechanical Loading ◽  
Bone Growth ◽  
Bone Matrix ◽  
Test Group ◽  
Control Group ◽  
Group A ◽  
Bone To Implant Contact ◽  
The Stability ◽  
Group B

Objectives: To determine the effect of mechanical loading of bone on the stability and histomorphometric variables of the osseointegration of dental implants using an experimental test in an animal model. Materials and Methods: A total of 4 human implants were placed in both tibiae of 10 New Zealand rabbits (n = 40). A 6-week osseointegration was considered, and the rabbits were randomly assigned to two groups: Group A (Test group) included 5 rabbits that ran on a treadmill for 20 min daily during the osseointegration period; Group B (Controls) included the other 5 that were housed conventionally. The monitored variables were related to the primary and secondary stability of the dental implants (implant stability quotient—ISQ), vertical bone growth, bone to implant contact (BIC), area of regenerated bone and the percentage of immature matrix. Results: The results of the study show a greater vertical bone growth (Group A 1.26 ± 0.48 mm, Group B 0.32 ± 0.47 mm, p < 0.001), higher ISQ values (Group A 11.25 ± 6.10 ISQ, 15.73%; Group B 5.80 ± 5.97 ISQ, 7.99%, p = 0.006) and a higher BIC (Group A 19.37%, Group B 23.60%, p = 0.0058) for implants in the test group, with statistically significant differences. A higher percentage of immature bone matrix was observed for implants in the control group (20.68 ± 9.53) than those in the test group (15.38 ± 8.84) (p = 0.108). A larger area of regenerated bone was also observed for the test implants (Group A 280.50 ± 125.40 mm2, Group B 228.00 ± 141.40 mm2), but it was not statistically significant (p = 0.121). Conclusions: The mechanical loading of bone improves the stability and the histomorphometric variables of the osseointegration of dental implants.

Transport of soluble organic and inorganic carbon in sandy soils under nitrogen fertilization

1999 ◽  
Vol 79 (2) ◽  
pp. 303-310 ◽  
Author(s):  
F. L. Wang ◽  
A. K. Alva
Keyword(s):  
Nitrogen Fertilization ◽  
Inorganic Carbon ◽  
Average Rate ◽  
Sandy Soils ◽  
Water Soluble ◽  
Total Carbon ◽  
Total Carbon Content ◽  
Water Soluble Organic Carbon ◽  
Isobutylidene Diurea ◽  
Leaching Condition

Leaching of water soluble soil carbon plays an important role in downward transport of soil nutrients and pollutants and may be influenced by soil and management factors. We examined the leaching of water soluble carbon from two sandy soils under nitrogen fertilization by adapting an intermittent leaching-incubation technique using packed soil columns (94 × 10 cm). After 30 d, cumulative amounts of water-soluble organic carbon (SOC) leached from the Candler and Wabasso sand for various treatments in mg C column−1 were: 77 and 302 (NH4NO3), 64 and 265 (control), and 45 and 239 (isobutylidene diurea, IBDU), respectively. The IBDU and NH4NO3 treatments increased the leaching of water-soluble inorganic carbon (SIC), which ranged from 2 to 38 mg C column−1 over 30 d. At the end of eight cycles of leaching/incubation, the total carbon content increased at depth (control and NH4NO3 treatment) in the Candler sand, but decreased in the Wabasso sand. In the first leaching event, the average rate of SOC leaching from the Wabasso sand was 26 mg C column−1 d−1 which dropped rapidly to about 5 mg C column−1 d−1 towards the end of the experiment. The rate of SOC leaching from the Candler sand was much lower (<8 mg C column−1 d−1) than the rate of SOC leaching from the Wabasso sand. Compared with the unamended treatments, application of NH4NO3 increased and IBDU decreased the leaching of SOC in both soils. These effects of N application were considerable during the initial two to three leaching events only. Our results suggest that the initial rainfalls that follow a dry period may be critical for transporting SOC from the upper layer of these sandy soils. Key words: C leaching, sandy soil, intermittent leaching condition, isobutylidene

scholarly journals Characteristics of Liparis loeselii (L.) Rich. populations in selected Natura 2000 areas in eastern Poland

2020 ◽  
Vol 55 (3) ◽  
pp. 151-162
Author(s):  
Danuta Urban ◽  
Joanna Sender ◽  
Ewelina Tokarz ◽  
Andrzej Różycki
Keyword(s):  
Natura 2000 ◽  
Total Carbon ◽  
Habitat Conditions ◽  
Total Carbon Content ◽  
Poor Fen ◽  
Calcareous Fen ◽  
Liparis Loeselii ◽  
The Individual ◽  
Insight Into

AbstractIn view of the sensitivity of Liparis loeselii to changes in habitat conditions, we carried out a study with the aim to monitor population numbers, identify the individual features of the Liparis loeselii population, analyse habitat conditions, identify threats and propose conservation measures to preserve the species. The investigations were conducted in seven unmanaged objects located in three Natura 2000 areas in eastern Poland. The results of this study provide a new insight into Liparis loeselii ecology. The analysed populations inhabited some habitat types: extremely poor fen, transitional mire, rich fen, calcareous fen, spring-fed fen. The content of nutrients was similar in all the habitats. A CCA analysis revealed that the total carbon content, pH, and redox potential of the substrate determine differences between the habitats analysed. Juvenile individuals represented a maximum of 12% of the analysed populations and were the least abundant group of these plants. The flowering was primarily influenced by hydrological conditions. Based on the long-term observations reported in this article, it can be assumed that the species stands a chance of surviving at the localities analysed, provided that the habitat conditions do not change dramatically.

Surface Studies of Coarse-Grained and Nanostructured Titanium Implants

2012 ◽  
Vol 12 (11) ◽  
pp. 8567-8572 ◽  
Author(s):  
D. M. Korotin ◽  
S. Bartkowski ◽  
E. Z. Kurmaev ◽  
M. Neumann ◽  
E. B. Yakushina ◽  
...  
Keyword(s):  
Titanium Implants ◽  
Coarse Grained ◽  
Surface Studies ◽  
Nanostructured Titanium

scholarly journals Archaeal ammonia oxidizers respond to soil factors at smaller spatial scales than the overall archaeal community does in a high Arctic polar oasis

2016 ◽  
Vol 62 (6) ◽  
pp. 485-491 ◽  
Author(s):  
Samiran Banerjee ◽  
Nabla Kennedy ◽  
Alan E. Richardson ◽  
Keith N. Egger ◽  
Steven D. Siciliano
Keyword(s):  
Community Structure ◽  
Spatial Scales ◽  
Archaeal Community ◽  
Diversity Indices ◽  
High Arctic ◽  
Total Carbon ◽  
Spatial Dependency ◽  
Edaphic Factors ◽  
Total Carbon Content ◽  
Arctic Soils

Archaea are ubiquitous and highly abundant in Arctic soils. Because of their oligotrophic nature, archaea play an important role in biogeochemical processes in nutrient-limited Arctic soils. With the existing knowledge of high archaeal abundance and functional potential in Arctic soils, this study employed terminal restriction fragment length polymorphism (t-RFLP) profiling and geostatistical analysis to explore spatial dependency and edaphic determinants of the overall archaeal (ARC) and ammonia-oxidizing archaeal (AOA) communities in a high Arctic polar oasis soil. ARC communities were spatially dependent at the 2–5 m scale (P < 0.05), whereas AOA communities were dependent at the ∼1 m scale (P < 0.0001). Soil moisture, pH, and total carbon content were key edaphic factors driving both the ARC and AOA community structure. However, AOA evenness had simultaneous correlations with dissolved organic nitrogen and mineral nitrogen, indicating a possible niche differentiation for AOA in which dry mineral and wet organic soil microsites support different AOA genotypes. Richness, evenness, and diversity indices of both ARC and AOA communities showed high spatial dependency along the landscape and resembled scaling of edaphic factors. The spatial link between archaeal community structure and soil resources found in this study has implications for predictive understanding of archaea-driven processes in polar oases.

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