scholarly journals RNA Identification and Detection of Nucleic Acids as Aerosols in Air Samples by Means of Photon and Electron Interactions

Instruments ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 23
Author(s):  
John I. Adlish ◽  
Piero Neuhold ◽  
Riccardo Surrente ◽  
Luca J. Tagliapietra
Keyword(s):  
Molecular Structure ◽  
Monte Carlo ◽  
Atomic Hydrogen ◽  
Air Samples ◽  
Viral Particles ◽  
Dna Strands ◽  
Cluster Configuration ◽  
Air Sample ◽  
Energy Channels ◽  
Electron Interactions

This study presents a methodology to reveal traces of viral particles, as aerosol with known chemical and molecular structure, in a sample by means of photon and electron interactions. The method is based on Monte Carlo simulations and on the analysis of photon-electron fluxes-spectra through energy channels counts as a function of different aerosol viral concentrations in the air sample and looking at the peculiar photon/electron interactions with the potential abnormal atomic hydrogen (H), oxygen (O), carbon (C), and phosphorus (P) compositions present in the air sample as a function of living and nonliving matter with PO4 group RNA/DNA strands in a cluster configuration.

scholarly journals RNA Identification and Detection of Nucleic Acids as Aerosols in Air Samples by Means of Photon and Electron Interactions

2021 ◽  
Author(s):  
Piero Neuhold ◽  
Luca J Tagliapietra
Keyword(s):  
Molecular Structure ◽  
Monte Carlo ◽  
Atomic Hydrogen ◽  
Air Samples ◽  
Viral Particles ◽  
Dna Strands ◽  
Cluster Configuration ◽  
Air Sample ◽  
Energy Channels ◽  
Electron Interactions

This study presents a methodology to reveal traces of viral particles, as aerosol with known chemical and molecular structure, in a sample by means of photon and electron interactions. The method is based on Monte Carlo simulations and on the analysis of photon-electron fluxes-spectra through energy channels counts as a function of different aerosol viral concentrations in the air sample and looking at the peculiar photon/electron interactions with the potential abnormal atomic hydrogen (H), oxygen (O), carbon (C), and phosphorus (P) compositions present in the air sample as a function of living and nonliving matter with PO4 group RNA/DNA strands in a cluster configuration.

scholarly journals Polyethylene Identification in Ocean Water Samples by Means of 50 Kev Energy Electron Beam

2020 ◽  
Author(s):  
John. I Adlish ◽  
Davide Costa ◽  
Enrico Mainardi ◽  
Piero Neuhold ◽  
Riccardo Surrente ◽  
...  
Keyword(s):  
Electron Beam ◽  
Photon Energy ◽  
Water Samples ◽  
Atomic Hydrogen ◽  
Contaminated Water ◽  
Water Fluxes ◽  
Ocean Water ◽  
Different Types ◽  
Dna Strands ◽  
Cluster Configuration

The study presented hereafter shows a new methodology to reveal traces of polyethylene (the most common microplastic particles, known as a structure of C2H4) in a sample of ocean water by the irradiation of a 50 keV, 1 µA electron beam. This is performed by analyzing the photon (produced by the electrons in water ) fluxes and spectra (i.e. fluxes as a function of photon energy) at different types of contaminated water with an adequate device and in particular looking at the peculiar interactions of electrons/photons with the potential abnormal atomic hydrogen (H), oxygen (O), carbon (C), phosphorus (P) compositions present in the water, as a function of living and not living organic organisms with a PO4 group RNA/DNA strands in a cluster configuration through a volumetric cells grid.

scholarly journals Polyethylene Identification in Ocean Water Samples by Means of 50 keV Energy Electron Beam

Instruments ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 32
Author(s):  
John I. Adlish ◽  
Davide Costa ◽  
Enrico Mainardi ◽  
Piero Neuhold ◽  
Riccardo Surrente ◽  
...  
Keyword(s):  
Electron Beam ◽  
Photon Energy ◽  
Water Samples ◽  
Atomic Hydrogen ◽  
Contaminated Water ◽  
Water Fluxes ◽  
Ocean Water ◽  
Different Types ◽  
Dna Strands ◽  
Cluster Configuration

This study presents a new methodology to reveal traces of polyethylene (the most common microplastic particles, known as a structure of C2H4) in a sample of ocean water by the irradiation of a 50 keV, 1 µA electron beam. This is performed by analyzing the photon (produced by the electrons in water) fluxes and spectra (i.e., fluxes as a function of photon energy) with different types of contaminated water using an adequate device and in particular looking at the peculiar interactions of electrons/photons with the potential abnormal atomic hydrogen (H), oxygen (O), carbon (C), and phosphorus (P) compositions present in the water, as a function of living and nonliving organic organisms with PO4 group RNA/DNA strands in a cluster configuration through a volumetric cells grid.

scholarly journals Heterogeneity of SARS-CoV-2 virus produced in cell culture revealed by shotgun proteomics and supported by genome sequencing

2021 ◽  
Author(s):  
Fabrice Gallais ◽  
Olivier Pible ◽  
Jean-Charles Gaillard ◽  
Stéphanie Debroas ◽  
Hélène Batina ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Cell Culture ◽  
Shotgun Proteomics ◽  
Rapid Analysis ◽  
Diagnostic Tools ◽  
Tandem Mass ◽  
The Past ◽  
Viral Particles ◽  
Depth Analysis ◽  
The Subject

AbstractCOVID-19 is the most disturbing pandemic of the past hundred years. Its causative agent, the SARS-CoV-2 virus, has been the subject of an unprecedented investigation to characterize its molecular structure and intimate functioning. While markers for its detection have been proposed and several diagnostic methodologies developed, its propensity to evolve and evade diagnostic tools and the immune response is of great concern. The recent spread of new variants with increased infectivity requires even more attention. Here, we document how shotgun proteomics can be useful for rapidly monitoring the evolution of the SARS-CoV-2 virus. We evaluated the heterogeneity of purified SARS-CoV-2 virus obtained after culturing in the Vero E6 cell line. We found that cell culture induces significant changes that are translated at the protein level, such changes being detectable by tandem mass spectrometry. Production of viral particles requires careful quality control which can be easily performed by shotgun proteomics. Although considered relatively stable so far, the SARS-CoV-2 genome turns out to be prone to frequent variations. Therefore, the sequencing of SARS-CoV-2 variants from patients reporting only the consensus genome after its amplification would deserve more attention and could benefit from more in-depth analysis of low level but crystal-clear signals, as well as complementary and rapid analysis by shotgun proteomics. Graphical abstract

CPU Issues in the Representation of the Molecular Structure of Petroleum Resid through Characterization, Reaction, and Monte Carlo Modeling

1994 ◽  
Vol 8 (3) ◽  
pp. 570-575 ◽  
Author(s):  
Thomas F. Petti ◽  
Daniel M. Trauth ◽  
Scott M. Stark ◽  
Matthew Neurock ◽  
Muzaffer Yasar ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Monte Carlo ◽  
Monte Carlo Modeling

Monte Carlo simulation of spatial distribution of atomic hydrogen in electron assisted CVD

2002 ◽  
Vol 11 (9) ◽  
pp. 1648-1652 ◽  
Author(s):  
Lifang Dong ◽  
Yuhong Zhang ◽  
Boqin Ma ◽  
Guangsheng Fu
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Spatial Distribution ◽  
Atomic Hydrogen

Nonelastic acoustic‐phonon‐electron interactions in Monte‐Carlo simulations at low fields

1984 ◽  
Vol 45 (6) ◽  
pp. 641-643 ◽  
Author(s):  
F. Hesto ◽  
J‐L. Pelouard ◽  
R. Castagné ◽  
J‐F. Pône
Keyword(s):  
Monte Carlo ◽  
Monte Carlo Simulations ◽  
Acoustic Phonon ◽  
Electron Interactions

Monte Carlo determination of the field dependence of the first Townsend ionization coefficient for atomic hydrogen

1970 ◽  
Vol 13 (8) ◽  
pp. 1040-1045
Author(s):  
V. B. Bolotin ◽  
N. P. Poshyunaite ◽  
L. Z. Fridlyand ◽  
G. E. Tokeris ◽  
A. B. Bolotin
Keyword(s):  
Monte Carlo ◽  
Field Dependence ◽  
Atomic Hydrogen ◽  
Ionization Coefficient

scholarly journals Toilets dominate environmental detection of SARS-CoV-2 virus in a hospital

2020 ◽  
Author(s):  
Zhen Ding ◽  
Hua Qian ◽  
Bin Xu ◽  
Ying Huang ◽  
Te Miao ◽  
...  
Keyword(s):  
Environmental Design ◽  
Environmental Sampling ◽  
Research Grants ◽  
Air Samples ◽  
Department Of Health ◽  
Surface Samples ◽  
Toilet Seat ◽  
Air Sample ◽  
Hospital Building ◽  
Expired Air

AbstractBackgroundRespiratory and faecal aerosols play a suspected role in transmitting the SARS-CoV-2 virus. We performed extensive environmental sampling in a dedicated hospital building for Covid-19 patients in both toilet and non-toilet environments, and analysed the associated environmental factors.MethodsWe collected data of the Covid-19 patients. 107 surface samples, 46 air samples, two exhaled condensate samples, and two expired air samples were collected were collected within and beyond the four three-bed isolation rooms. We reviewed the environmental design of the building and the cleaning routines. We conducted field measurement of airflow and CO2 concentrations.FindingsThe 107 surface samples comprised 37 from toilets, 34 from other surfaces in isolation rooms (ventilated at 30-60 L/s), and 36 from other surfaces outside isolation rooms in the hospital. Four of these samples were positive, namely two ward door-handles, one bathroom toilet-seat cover and one bathroom door-handle; and three were weakly positive, namely one bathroom toilet seat, one bathroom washbasin tap lever and one bathroom ceiling-exhaust louvre. One of the 46 air samples was weakly positive, and this was a corridor air sample. The two exhaled condensate samples and the two expired air samples were negative.InterpretationThe faecal-derived aerosols in patients’ toilets contained most of the detected SARS-CoV-2 virus in the hospital, highlighting the importance of surface and hand hygiene for intervention.FundingThe work were partially supported by the National Natural Science Foundation of China (no 41977370), the Research Grants Council of Hong Kong’s (no 17202719) (no C7025-16G), and Scientific Research Fund of Jiangsu Provincial Department of Health (no S21017002).

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