scholarly journals Monitoring Resistance of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay

Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 599
Author(s):  
Cristiane Maria Tibola ◽  
Leandro Silva ◽  
Fernanda Sgubin ◽  
Celso Omoto

The novel ingestion bioassay method was developed for detecting and monitoring resistance of Euschistus heros by encapsulating an artificial liquid diet using Parafilm®. This methodology was compared with the tarsal contact (vial test) and topical application methods for thiamethoxam, imidacloprid, and lambda-cyhalothrin. The best bioassay method for the neonicotinoid insecticides thiamethoxam and imidacloprid was ingestion. For pyrethroid insecticide lambda-cyhalothrin, the best result was obtained by topical application. Using the best bioassay method for each insecticide, the susceptibility to these insecticides was monitored in 30 populations of E. heros collected from soybean crops in Brazil from 2018 to 2020. High variations in susceptibility to thiamethoxam (resistance ratios, 1.6–22 times), imidacloprid (resistance ratios, 1.6–22 times), and lambda-cyhalothrin (resistance ratios, 5–40 times) were detected among the evaluated E. heros populations. In order to monitor the susceptibility of E. heros to insecticides, diagnostic concentrations were defined based on the LC99 of the susceptible reference population: 5.65 µL of a.i./mL for thiamethoxam, 12.45 µL of a.i./mL for imidacloprid, and 0.20 µg of a.i./insect for lambda-cyhalothrin. Subsequently, we select an E. heros strain resistant to neonicotinoid insecticides and another to lambda-cyhalothrin. The resistance ratios obtained after seven selection cycles were 66, 41 and 44 times for thiamethoxam, imidacloprid and lambda-cyhalothrin, respectively.

2021 ◽  
Vol 51 ◽  
Author(s):  
Samanta Souza Restelatto ◽  
Paulo Eduardo Wille ◽  
Nayara Buss ◽  
Cleiton Luis Wille ◽  
Mari Inês Carissimi Boff ◽  
...  

ABSTRACT The looper caterpillar Chrysodeixis includens (Walker) is considered the main defoliating pest in soybean crops, which contributes to yield losses. Delaying its resistance to insecticides is a major challenge in its management. This study aimed to characterize the susceptibility of C. includens to flubendiamide, cyantraniliprole, spinetoram and methomyl, as well as to establish a diagnostic concentration for monitoring resistance. The insecticide was applied to the surface of the artificial diet. For the dose-response curve, concentrations that resulted in 5 to 99 % mortality were used to estimate the lethal concentrations (LC50 and LC99) and resistance ratios (RR50 and RR99). The diagnostic concentrations were based on concentrations that provided 90 to 99 % mortality in the susceptible reference population. For flubendiamide and methomyl, the decrease in the susceptibility resulted in estimated RR50 of 6.2 to 24.2 and 4.4 to 19.6 times, respectively. For cyantraniliprole and spinetoram, there was little difference in susceptibility among the populations, with RR99 lower than 6.1 times. Differences in the susceptibility of C. includens were evident from concentrations of 0.5053, 5.053, 0.1579 and 28.42 μg cm-2, respectively for flubendiamide, cyantraniliprole, spinetoram and methomyl.


2018 ◽  
Vol 10 (12) ◽  
pp. 185 ◽  
Author(s):  
Elidiane Feltrin-Campos ◽  
Marcos Gino Fernandes ◽  
Gabrielle de Lima Masson ◽  
Thaís Araujo Corrêa ◽  
José Fernando Jurca Grigolli

Use of synthetic insecticides remains main control strategy for percevejo-marrom (Euschistus heros) in corn. However, indiscriminate use of these products can have adverse effects on the environment and human health. Thus, incorporation of bio-agents like Telenomus podisi a preferred option in corn pest management. Study was conductedto evaluate the effects of insecticides application on T. podisi mortality rates, the number of eggs parasitized by the F0 generation, and number of emergent F1 parasitoids. The insecticides tested were: imidacloprid+bifenthrin, bifenthrin+carbosulfan, thiametoxam+lambda-cyhalothrin, acephate, bifenthrin, and imidacloprid at the highest concentrations indicated for corn. Each insecticide was classified according to its selectivity, based on the methodology proposed by IOBC/WPRS. Only imidacloprid did not affect parasitoid survival however the parasitism rates of F0 females, were affected, classifying this product as slightly harmful for this biological trait. Regarding parasitoid emergence (F1), thiametoxam+lambda-cyhalothrin was classified as moderately harmful, while bifenthrin+carbosulfan and bifenthrin were considered harmful, no parasitism occurred. Thus the evaluated insecticides were classified as slightly harmful to the parasitoid T. podisi under laboratory conditions for the biological characteristics evaluated. Further studies under semi-field and field conditions are needed in order to confirm these toxicity levels on T. podisi.


1994 ◽  
Vol 44 (3) ◽  
pp. 277-285
Author(s):  
Akiko NISHIDA ◽  
Mizuo KANI ◽  
Shunji IKUTA ◽  
Kenji YOKOI ◽  
Atsunori ISOZAKI ◽  
...  

2017 ◽  
Vol 20 (1) ◽  
pp. 279-287
Author(s):  
Rachel Colauto Milanezi Aguiar ◽  
Patrícia Elizabeth Husch ◽  
Raquel Bozini Gallo ◽  
Sheila Michele Levy ◽  
Laurival Antônio Vilas-Bôas ◽  
...  

2021 ◽  
pp. 1-8
Author(s):  
Gunbharpur Singh Gill ◽  
Juang Horng Chong

Management of sweetpotato whitefly (Bemisia tabaci), one of the most economically important pests of poinsettia (Euphorbia pulcherrima), relies heavily on neonicotinoid insecticides. Growers are seeking insecticide alternatives to neonicotinoids due to market demands. Although several systemic and translaminar insecticides have been suggested as alternatives to neonicotinoids, no published study has simultaneously compared their efficacies against sweetpotato whiteflies. This study compared the efficacies of 10 systemic and translaminar alternative insecticides with those of two systemic neonicotinoids, when all products were applied as foliar spray (twice at 14 d) or substrate drench (once) against sweetpotato whiteflies on poinsettia plants. Sweetpotato whitefly nymph and adult densities were examined 2 weeks before the first application (pretreatment), and weekly after the application for 8 weeks. Results showed that insecticides varied greatly in their efficacy, particularly against adults, and that spray application provided more effective suppression of nymphs than drench application. Spray and drench applications of imidacloprid and dinotefuran were consistently the most effective against sweetpotato whitefly nymphs and adults. Among the neonicotinoid alternatives, cyantraniliprole was the most effective insecticide in reducing sweetpotato whitefly nymph densities by both spray and drench application methods, with efficacy comparable to those of imidacloprid and dinotefuran. Although less effective than cyantraniliprole, foliar sprays of afidopyropen, chloratraniliprole, cyclaniliprole, flonicamid, flupyradifurone, pyrifluquinazon, spirotetramat, and sulfoxaflor + spinetoram were also effective against nymphs and could serve as partners in an insecticide rotation program.


2007 ◽  
Vol 53 (2) ◽  
pp. 187-194 ◽  
Author(s):  
Andrew B. Barron ◽  
Joanna Maleszka ◽  
Robert K. Vander Meer ◽  
Gene E. Robinson ◽  
Ryszard Maleszka

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