scholarly journals Effects of Insecticide Stress on Expression of NlABCG Transporter Gene in the Brown Planthopper, Nilaparvata lugens

Insects ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 334 ◽  
Author(s):  
Yang ◽  
Zhou ◽  
Yang ◽  
Long ◽  
Jin
Keyword(s):  
Molecular Mechanisms ◽  
Transmembrane Domain ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Molecular Transporters ◽  
Real Time Quantitative Pcr ◽  
Important Pest ◽  
Baseline Information ◽  
Response To Stress ◽  
Important Means

The brown planthopper (BPH), Nilaparvata lugens, is an important pest of rice that severely affects production. Insecticides are an important means of controlling BPH, but their long-term use has led to resistance. To provide insight into BPH responses to insecticide stress, we determined the expression levels of BPH ABCG transporter genes under treatment with thiamethoxam, abamectin, and cyantraniliprole at LC10, LC25, LC50, and LC90. We cloned 13 BPH ABCG transporters, named NlABCG1 to NlABCG13. Conservative domain analysis showed that all 13 transporters have one nucleotide binding domain and one transmembrane domain, typical of semi-molecular transporters. Real-time quantitative PCR showed that thiamethoxam, abamectin, and cyantraniliprole stress increased the expression of some NlABCG transporters gene in BPH. However, after treatment with thiamethoxam at LC25 and abamectin at LC10, there was no significant upregulation of NlABCG. These results indicate that the expression of NlABCG varies in response to stress from different insecticides. These findings provide baseline information for further understanding of the molecular mechanisms of insecticide resistance in BPH.

scholarly journals Identification and analysis of miRNAs in IR56 rice in response to BPH infestations of different virulence levels

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Satyabrata Nanda ◽  
San-Yue Yuan ◽  
Feng-Xia Lai ◽  
Wei-Xia Wang ◽  
Qiang Fu ◽  
...  
Keyword(s):  
Regulatory Network ◽  
Molecular Mechanisms ◽  
Brown Planthopper ◽  
Enrichment Analysis ◽  
Transcript Abundance ◽  
Defense Responses ◽  
Nilaparvata Lugens ◽  
Mirna Sequence ◽  
Rna Profiling ◽  
Underlying Mechanisms

Abstract Rice production and sustainability are challenged by its most dreadful pest, the brown planthopper (Nilaparvata lugens Stål, BPH). Therefore, the studies on rice-BPH interactions and their underlying mechanisms are of high interest. The rice ontogenetic defense, such as the role of microRNAs (miRNAs) has mostly been investigated against the pathogens, with only a few reports existing against the insect infestations. Thus, revealing the involvement of rice miRNAs in response to BPH infestations will be beneficial in understanding these complex interactions. In this study, the small RNA profiling of the IR56 rice in response to separate BPH infestations of varied virulence levels identified the BPH-responsive miRNAs and revealed the differential transcript abundance of several miRNAs during a compatible and incompatible rice-BPH interaction. The miRNA sequence analysis identified 218 known and 28 novel miRNAs distributed in 54 miRNA families. Additionally, 138 and 140 numbers of differentially expressed (DE) miRNAs were identified during the compatible and incompatible interaction, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed the target gene candidates of DE miRNAs (including osa-miR2871a-3p, osa-miR172a, osa-miR166a-5p, osa-miR2120, and osa-miR1859) that might be involved in the IR56 rice defense responses against BPH infestation. Conversely, osa-miR530-5p, osa-miR812s, osa-miR2118g, osa-miR156l-5p, osa-miR435 and two of the novel miRNAs, including novel_16 and novel_52 might negatively modulate the IR56 rice defense. The expressional validation of the selected miRNAs and their targets further supported the IR56 rice defense regulatory network. Based on our results, we have proposed a conceptual model depicting the miRNA defense regulatory network in the IR56 rice against BPH infestation. The findings from the study add further insights into the molecular mechanisms of rice-BPH interactions and will be helpful for the future researches.

scholarly journals EFFECTS OF POPULATION DENSITY AND HOST AVAILABILITY ON THE MIGRATION PROCESS OF BROWN PLANTHOPPER FED USING SUSCEPTIBLE AND RESISTANT RICE VARIETIES (PENGARUH KEPADATAN POPULASI DAN KETERSEDIAAN PAKAN TERHADAP PROSES MIGRASI WERENG BATANG COKELAT PADA PADI VARIETAS RENTAN DAN TAHAN)

2017 ◽  
Vol 20 (2) ◽  
pp. 72
Author(s):  
Imam Habibi ◽  
Witjaksono Witjaksono ◽  
Arman Wijonarko
Keyword(s):  
Population Density ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Susceptible Variety ◽  
Resistant Variety ◽  
Rice Varieties ◽  
Host Availability ◽  
Important Pest ◽  
Nilaparvata Lugens Stål ◽  
Field Failure

Brown planthopper, Nilaparvata lugens Stal. (Hemiptera: Delphacidae), is an important pest of rice. This pest can cause hopperburn and field failure. This research aimed to determine the effects of population density and host availability on migration of N. lugens. The criteria used to justify the effects of host availability and population density on migration of N. lugens were based the hardness and tannin tests of the rice stems, fecundity of N. lugens, and the life cycle of N. lugens. The research was conducted under the temperature of 29.42°C with relative humidity of 61% and Light 12: Dark 12 times, using ten pairs of N. lugens brachypterous (F0 constant) and then was added with five male adults on fifth days after the first infestation (F0 changed). The varieties used were IR64, as a resistant variety, and Ketan Lusi, as a susceptible variety. The results showed that the adding of the macropterous males did not affect the number of macropterous, because of that has been preplanned by the F0. Therefore, the percentage of existing macropterous was 51−52%. Wereng Batang Cokelat (WBC) merupakan salah satu hama tanaman padi yang sangat penting. Kerusakan parah dapat menyebabkan hopperburn dan puso (gagal panen). Tujuan penelitian ini adalah mengetahui pengaruh kepadatan populasi dan tanaman inang sebagai tempat migrasi WBC. Parameter yang dikaji untuk mengetahui pengaruh kepadatan populasi WBC dan tanaman inang tempat migrasi WBC berdasarkan tingkat kekerasan dan kandungan tanin batang tanaman padi, fekunditas WBC, dan siklus hidup WBC. Penelitian ini dilakukan pada temperatur 29.42˚C dengan kelembapan relatif 61% dan durasi siang hari 12 jam: durasi malam hari 12 jam. Metode yang dilakukan adalah dengan menggunakan 10 pasang imago WBC brakhiptera (F0 konstan), kemudian dilakukan penambahan 5 ekor imago jantan pada hari kelima setelah infestasi awal (F0 diubah). Varietas padi yang digunakan yaitu padi varietas IR64 sebagai varietas tahan dan ketan Lusi sebagai varietas rentan. Hasil penelitian menunjukkan bahwa penambahan imago jantan makroptera tidak berpengaruh terhadap jumlah keturunan makroptera yang dihasilkan, karena imago (F0) telah merencanakan terlebih dahulu keturunan yang akan dihasilkan. Oleh karena itu, persentase terbentuk keturunan imago makroptera berkisar antara 51−52%. 

scholarly journals Salivary Protein NlSP7 of The Brown Planthopper Functions As An Effector for Mediating Tricin Metabolism in Rice Plants

2021 ◽  
Author(s):  
Gu Gong ◽  
Long-Yu Yuan ◽  
Yi-Feng Li ◽  
Hang-Xiang Xiao ◽  
Yan-Fang Li ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Food Intake ◽  
Rice Plant ◽  
Artificial Diet ◽  
Mrna Level ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Salivary Protein ◽  
Rice Plants ◽  
Important Pest

Abstract The brown planthopper (BPH), Nilaparvata lugens, is an important pest that affects rice (Oryza sativa) production in Asia. The flavone tricin (5,7,4ʹ-trihydroxy-3ʹ,5ʹ-dimethoxy flavone) is a valuable secondary metabolite commonly found in rice plants that can defend rice plants against infestation by BPH. BPH damage can reduce the metabolic level of tricin in rice. Our preliminary transcriptome research results showed that BPH salivary protein 7, NlSP7, is highly responsive to tricin stimuli. However, the function of NlSP7 in mediating the interaction between the rice plant and the BPH is unknown. In this study, we cloned the NlSP7 gene in N. lugens and found that its mRNA level was greater in the presence of high tricin content than low tricin content, regardless of whether the BPHs were fed a rice plant diet or an artificial diet containing 100 mg/L tricin. Knocking down NlSP7 resulted in BPH individuals spending more time in the non-penetration and pathway phase, and less time feeding on the phloem of rice plants. These changes decreased BPH food intake, feeding behavior, and fitness, as well as the tricin content of the rice plants. These findings demonstrate that the salivary protein NlSP7 of BPH functions as an effector for tricin metabolism in rice.

scholarly journals Parasitism of The Rice Brown Planthopper Eggs in Various Periods of Time of The Day

2016 ◽  
Vol 20 (1) ◽  
pp. 28
Author(s):  
Siti Haryati ◽  
Y. Andi Trisyono ◽  
Witjaksono Witjaksono
Keyword(s):  
Cultural Practices ◽  
Early Stage ◽  
Brown Planthopper ◽  
Early Morning ◽  
Nilaparvata Lugens ◽  
Before And After ◽  
Important Pest ◽  
Parasitism Level ◽  
Peak Abundance ◽  
Rice Ecosystem

The rice brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae) is an important pest of rice. Since at the early stage, this pest is infested by parasitoids, but most cultural practices do not consider the existences of parasitoids in rice ecosystem. This study was aimed to determine the level of parasitism on N. lugens with regard to the time of the day. This information would be useful to minimize the effect of insecticide application to the parasitoids. Trapping  of  egg parasitoids in rice ecosystem was conducted every two hours from 05.00 a.m. until 04.00 p.m. Parasitism occured as early as at 05.00 a.m. (12.26% of the total parasitoid found), reached the peak abundance at 11.00 a.m. (36.13%), and decreased at 01.00 p.m. The parasitism level varied from 1.12 to 8.51% at 66 days after planting. The highest number of parasitoids and the highest parasitism level occured when trapping was conducted between 11.00 a.m.−01.00 p.m.. Before and after this period of time, the number of parasitoid emerged and parasitism were low. This suggest that if insecticide is necessary, it should be applied in the early morning or late afternoon. INTISARIWereng batang padi cokelat, Nilaparvata lugens (Hemiptera: Delphacidae), merupakan salah satu hama penting padi. Praktik budidaya pertanian padi belum memperhatikan aktivitas parasitoid dalam ekosistem. Tujuan dari penelitian ini yaitu untuk mengetahui tingkat parasitasi telur N. lugens pada beberapa waktu dalam sehari. Informasi ini akan bermanfaat untuk mengurangi dampak aplikasi insektisida terhadap parasitoid. Pemerangkapan parasitoid telur N. lugens di lahan padi dilakukan setiap dua jam yang dimulai pada pukul 05.00 sampai pukul 16.00. Parasitoid telur N. lugens mulai aktif pada pukul 05.00 (12,26%), mencapai puncaknya pukul 11.00 (36,13%), dan mulai mengalami penurunan pada pukul 13.00. Tingkat parasitasi pada tanaman padi umur 66 hari setelah tanam berkisar mulai 1,12−8,51%. Hasil pemerangkapan menunjukkan bahwa jumlah parasitoid yang paling banyak muncul dan tingkat parasitisme tinggi terjadi pada waktu pemerangkapan yang dilakukan pada pukul 11.00−13.00. Sebelum dan sesudah waktu pemerangkapan tersebut jumlah parasitoid dan tingkat parasitisme rendah. Hal ini menunjukkan bahwa apabila aplikasi insektisida diperlukan, maka harus dilakukan di pagi hari atau sore hari. 

scholarly journals Evaluation of Rice Landraces for Brown Planthopper Resistance Based on Phenotypic Reactions and Biochemical Attributes

2021 ◽  
Author(s):  
Debashis Roy ◽  
Abhisek Biswas ◽  
Sukamal Sarkar ◽  
Gautam Chakraborty ◽  
Pijush Kanti Sarkar
Keyword(s):  
Feeding Rate ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Egg Hatchability ◽  
Rice Landraces ◽  
Indian Origin ◽  
Important Pest ◽  
Brown Planthopper Resistance ◽  
Adult Preference ◽  
Moderately Resistant

Abstract Brown planthopper (BPH), [Nilaparvata lugens (Stål.)] is an economically important pest of rice (Oryza sativa L.) throughout Asia, where the damage caused by nymphs and adults, especially during post-tillering to milking stages, significantly reduces grain yield. There is, thus, a pressing need to develop varieties that are resistant to BPH. In this study, the reaction of various rice landraces from Indian origin were assessed (both phenotypically and biochemically) in response to BPH infestation. It was found that the landraces, viz. Badshabhog, Gamra, Haldichuri, Janglijata, Kalabhat, Khara, Adanshilpa, Chikonmashuri, Kerala sundari and Lal dudheshwar exhibited resistance to BPH consistently along with the standard check Ptb33, for three consecutive years under both greenhouse and open-field conditions. These phenotypically resistant rice landraces including Ptb33 exhibited lowest feeding rate, least nymphal and adult preference, minimum survival and higher frequency (%) of unhatched eggs when compared with the susceptible check (Swarna). Higher levels ascorbic acid, oxalic acid (OA), crude silica (CS), while lower levels of phenols, reducing sugar and total free amino acid (TFA) were expressed in un-infested resistant and moderately resistant landraces. The resistant plants exposed to herbivory by BPH produced higher levels of phenolic compounds, potassium and TFA than plants of susceptible cultivar Swarna. The feeding rate, settling behaviour and survivability of BPH correlated significantly and negatively with OA and CS, whereas the latter showed a significant and positive correlation with egg hatchability.

scholarly journals Phenotypic and transcriptomic responses of two Nilaparvata lugens populations to the Mudgo rice containing Bph1

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Pin-Jun Wan ◽  
Ruo-Nan Zhou ◽  
Satyabrata Nanda ◽  
Jia-Chun He ◽  
San-Yue Yuan ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Model Systems ◽  
Plant Interactions ◽  
Electrical Penetration Graph ◽  
Incompatible Interaction ◽  
Rice Varieties ◽  
P450 Gene ◽  
Transcriptomic Responses

Abstract The Bph1 gene was the first reported brown planthopper (BPH, Nilaparvata lugens) resistance gene in Mudgo rice and was widely used as a commercial cultivar for controlling BPH infestations. However, rapid adaptations of BPH on the Mudgo rice resulted in its resistance breakdown and the emergence of virulent BPH populations. Thus, specific BPH populations and rice varieties can serve as good model systems for studying the roles of different bio-compounds and proteins in the insect-plant interactions. Although our understandings have been improved on the complexity of BPH and rice interactions, the underlying molecular mechanisms remain largely unknown. Here we analyzed the feeding performances and the transcriptomic responses of two BPH populations (Mugdo-BPH and TN1-BPH) during compatible (Mudog-BPH feeding on Mudgo rice) and incompatible (TN1-BPH feeding on Mudgo rice) interactions. The electrical penetration graph (EPG) results indicated that the BPH feeding and performances during the incompatible interaction are significantly affected in terms of decreased honeydew, loss of weight, decreased phloem sap ingestion (N4 waveform), but increased non-penetration (NP waveform) phase. Abundance of glucose and trehalose was reduced in BPH during the incompatible interaction. Transcriptomic surveys of insects in both interactions revealed that genes involved in cuticle formation, detoxification, metabolite transport, digestion, RNA processing, lipid or fatty acid metabolism, and proteolysis were significantly down-regulated during the incompatible interaction, whereas genes involved in insulin signaling were significantly upregulated. Knockdown of four genes, including the sugar transporter NlST45, the serine and arginine-rich protein NlSRp54, the cytochrome P450 gene NlCYP6AY1, and the cuticle protein NlCPR70 through RNA-interference revealed thess genes are important for BPH survival. Overall, the results of this study will be helpful for the future researches on BPH virulence shifts.

scholarly journals Transcriptomic Analysis of the Brown Planthopper, Nilaparvata lugens, at Different Stages after Metarhizium anisopliae Challenge

Insects ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 139
Author(s):  
Yifan Peng ◽  
Jifeng Tang ◽  
Jiaqin Xie
Keyword(s):  
Fungal Infection ◽  
Metarhizium Anisopliae ◽  
Time Course ◽  
Molecular Mechanisms ◽  
Control Strategies ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Potential Control ◽  
Clusters Of Orthologous Groups ◽  
Illumina Deep Sequencing

Nilaparvata lugens is one of the major pests of rice and results in substantial yield loss every year. Our previous study found that the entomopathogenic fungus Metarhizium anisopliae showed effective potential for controlling this pest. However, the mechanisms underlying M. anisopliae infection of N. lugens are not well known. In the present study, we further examined the transcriptome of N. lugens at 4 h, 8 h, 16 h, and 24 h after M. anisopliae infection by Illumina deep sequencing. In total, 174.17 Gb of data was collected after sequencing, from which 23,398 unigenes were annotated by various databases, including 3694 newly annotated genes. The results showed that there were 246 vs 75, 275 vs 586, 378 vs 1055, and 638 vs 182 up- and downregulated differentially expressed genes (DEGs) at 4 h, 8 h, 16 h, and 24 h after M. anisopliae infection, respectively. The biological functions and associated metabolic processes of these genes were determined with the Clusters of Orthologous Groups (COG), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The DEGs data were verified using RT-qPCR. These results indicated that the DEGs during the initial fungal infection appropriately reflected the time course of the response to the fungal infection. Taken together, the results of this study provide new insights into the molecular mechanisms underlying the insect host response to fungal infection, especially during the initial stage of infection, and may improve the potential control strategies for N. lugens.

scholarly journals A Salivary Odorant-Binding Protein Mediates Nilaparvata lugens Feeding and Host Plant Phytohormone Suppression

2021 ◽  
Vol 22 (9) ◽  
pp. 4988
Author(s):  
Hao Liu ◽  
Chao Wang ◽  
Chang-Lai Qiu ◽  
Jin-Hua Shi ◽  
Ze Sun ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Transcriptome Sequencing ◽  
Rice Plant ◽  
Host Location ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Insect Host ◽  
Odorant Binding Proteins ◽  
Real Time Quantitative Pcr ◽  
Odorant Binding

Odorant-binding proteins (OBPs) typically act as transporters of odor molecules and play an important role in insect host location. Here, we identified an OBP in brown planthopper (BPH) Nilaparvata lugens salivary glands via transcriptome sequencing. Real-time quantitative PCR and Western blotting analysis results showed that NlugOBP11 was highly expressed in salivary glands and secreted into rice plant during feeding, suggesting that it assists in BPH feeding on rice. Functional analysis in N. lugens saliva revealed that silencing this gene by RNA interference decreased the BPH stylet performance in the phloem of rice plants, reduced sap sucking, and ultimately led to insect death. Moreover, overexpression of NlugOBP11 in rice protoplasts or Nicotiana benthamiana leaves inhibited the production of defense-related signaling molecule salicylic acid in rice plant. The results demonstrate that NlugOBP11 is not only essential for BPH feeding, but also acts as an effector that inhibits plant defense.

scholarly journals Respon Hama Wereng Coklat Nilaparvata lugens Stal. (Hemiptera: Delphacidae) Terhadap Ketahanan Dan Kerentanan Varietas Padi

2020 ◽  
Vol 16 (1) ◽  
pp. 51-55
Author(s):  
Rein Estefanus Senewe ◽  
Silvia Permatasari ◽  
Marietje Pesireron
Keyword(s):  
Filter Paper ◽  
Plant Protection ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Rice Varieties ◽  
Rice Plants ◽  
Average Value ◽  
Important Pest ◽  
Nilaparvata Lugens Stål ◽  
Filter Papers

Brown planthopper (BPH) is one of the important pest insects that attack rice plants, which indirectly becomes a vector for the spread of grassy dwarf and empty dwarf diseases caused by viruses, and can consume a lot of food in a short time so that it can cause damage explosions and great losses. The aim of this study was to compare the eating response of brown planthopper to resistant and vulnerable rice varieties through the measurement of secreted honeydews. The study was conducted at the Toxicology Laboratory, Department of Plant Protection, Faculty of Agriculture, Bogor Agricultural University. Rice seedlings for honeydew testing were 30 days after sowing. Testing was done by infesting 3 female BPH into a plastic cage with filter paper (Whatman No.40 9 cm in diameter) placed on the bottom, which had been sprayed with a solution of Ninhydrin 0.01 mg/ml in acetone. The treatment consisted of 3 rice varieties (Ciherang, IR-64 dan Pelita) on each ovened and non-ovened filter paper, with three replicates so that a total of 18 treatments were used by infesting each with the 3 female BPH nymphs. Whereas, 3 treatments with non-ovened papers, with 3 replicates, were infested with 3 female BPH imago each. So that the treatments involved a total of 27 feeding tubes. Honeydews released by BPH that ate on the test varieties for 24 hours were collected on filter papers and formed blue/purple spots. The extent of the honeydew spots formed was measured and analyzed. The results showed that the average value of phloem consumption in Ciherang, IR-64, and Pelita rice varieties showed that these three varieties were very susceptible to BPH sucking pests. The more food nutrients sucked by BPH pests from rice plants, the greater the number of honeydew spots and the wider the honeydew spots that were produced. The areas of honeydew spots of Pelita variety was 33.78 m2, IR-64 28.26 mm2, and Ciherang 22.44 mm2, respectively. Pelita rice varieties had a high susceptibility to BPH pests when compared to Ciherang and IR-64 because Pelita does not have resistance genes to BPH pests. Keywords: Ciherang, IR-64, Pelita, planthopper, rice, honeydew   ABSTRAK Wereng batang coklat (WBC) merupakan salah satu serangga hama penting yang menyerang tanaman padi, yang secara tidak langsung menjadi vektor bagi penyebaran penyakit kerdil rumput dan kerdil hampa oleh virus, serta dapat mengkonsumsi makanan yang banyak dalam waktu singkat sehingga dapat menimbulkan ledakan kerusakan dan kerugian yang besar. Penelitian ini bertujuan untuk membandingkan respon makan WBC pada varietas padi tahan dan rentan melalui pengukuran embun madu yang disekresikan. Penelitian dilaksanakan di Laboratorium Toksikologi, Departemen Proteksi Tanaman Fakultas Pertanian, Institut Pertanian Bogor. Bibit padi untuk pengujian embun madu digunakan saat berumur 30 hari setelah semai. Pengujian dengan menginfestasikan WBC betina ke dalam kurungan plastik yang di bagian bawahnya diletakkan kertas saring (Whatman No. 40 berdiameter 9 cm) yang telah disemprot dengan larutan Ninhidrin 0,01 mg/mL aseton. Perlakuan yang terdiri dari 3 varietas padi (Ciherang, IR-64 dan Pelita) pada masing-masing kertas saring oven dan tidak oven dan dengan tiga ulangan sehingga total terdapat 18 perlakuan dengan menginfestasikan masing-masing 3 ekor nimfa betina WBC. Sedangkan pada 3 perlakuan kertas saring tanpa oven, dengan tiga ulangan, yang diinfestasikan masing-masing berupa 3 ekor imago betina WBC. Sehingga total perlakuan seluruhnya meliputi sebanyak 27 tabung makan. Embun madu yang dikeluarkan oleh WBC yang makan pada varietas uji selama 24 jam tertampung pada kertas saring dan membentuk bercak berwarna biru/ungu. Luas bercak embun madu yang terbentuk diukur dan dianalisis. Hasil penelitian menunjukkan bahwa nilai rata-rata tingkat konsumsi floem pada varietas padi Ciherang, IR-64 dan Pelita, menunjukkan bahwa ketiga varietas ini sangat rentan terhadap hama pengisap WBC. Semakin banyak nutrisi makanan yang disedot oleh hama WBC dari tanaman padi, maka semakin banyak jumlah spot bercak honeydew dan semakin luas spot bercak honeydew yang dihasilkan. Luas spot untuk varietas Pelita adalah 33,78 m2, IR-64 28,26 mm2, dan Ciherang 22,44 mm2. Varietas padi Pelita memiliki kerentanan yang tinggi terhadap hama WBC, bila dibandingkan dengan varietas Ciherang dan IR-64, karena varietas Pelita ini tidak memiliki gen ketahanan terhadap hama WBC. Kata kunci: Ciherang, honeydew IR-64, padi, Pelita, wereng

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