scholarly journals Zein-Based Films Containing Monolaurin/Eugenol or Essential Oils with Potential for Bioactive Packaging Application

2021 ◽  
Vol 23 (1) ◽  
pp. 384
Author(s):  
Jana Sedlarikova ◽  
Magda Janalikova ◽  
Petra Peer ◽  
Lucie Pavlatkova ◽  
Antonin Minarik ◽  
...  
Keyword(s):  
Essential Oils ◽  
Morphological Characteristics ◽  
Barrier Properties ◽  
Ethanol Solution ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Casting Technique ◽  
Thermal Techniques ◽  
Yeasts And Molds ◽  
Oil Films

Zein is renewable plant protein with valuable film-forming properties that can be used as a packaging material. It is known that the addition of natural cross-linkers can enhance a film’s tensile properties. In this study, we aimed to prepare antimicrobial zein-based films enriched with monolaurin, eugenol, oregano, and thyme essential oil. Films were prepared using the solvent casting technique from ethanol solution. Their physicochemical properties were investigated using structural, morphological, and thermal techniques. Polar and dispersive components were analyzed using two models to evaluate the effects on the surface free energy values. The antimicrobial activity was proven using a disk diffusion method and the suppression of bacterial growth was confirmed via a growth kinetics study with the Gompertz function. The films’ morphological characteristics led to systems with uniform distribution of essential oils or eugenol droplets combined with a flat-plated structure of monolaurin. A unique combination of polyphenolic eugenol and amphiphilic monoglyceride provided highly stretchable films with enhanced barrier properties and efficiency against Gram-positive and Gram-negative bacteria, yeasts, and molds. The prepared zein-based films with tunable surface properties represent an alternative to non-renewable resources with a potential application as active packaging materials.

scholarly journals Evaluation of the in vitro antibacterial activity of some essential oils and their blends against Staphylococcus spp. isolated from episodes of sheep mastitis

2021 ◽  
Author(s):  
Filippo Fratini ◽  
Margherita Giusti ◽  
Simone Mancini ◽  
Francesca Pisseri ◽  
Basma Najar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Diffusion Method ◽  
Minimal Bactericidal Concentration ◽  
Coagulase Negative Staphylococci ◽  
Disk Diffusion Method ◽  
In Vitro Antibacterial Activity ◽  
High Concentrations ◽  
Thymus Serpyllum

AbstractStaphylococcus aureus and coagulase-negative staphylococci are among the major causes of mastitis in sheep. The main goal of this research was to determine the in vitro antibacterial activity of several essential oils (EOs, n 30), then five of them were chosen and tested alone and in blends against staphylococci isolates. Five bacteria were isolated from episodes of ovine mastitis (two S. aureus and three S. xylosus). Biochemical and molecular methods were employed to identify the isolates and disk diffusion method was performed to determine their antimicrobial-resistance profile. The relative percentage of the main constituents in the tested essential oils and their blends was detected by GC-EIMS analysis. Antibacterial and bactericidal effectiveness of essential oils and blends were evaluated through minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). All of them showed sensitivity to the used antimicrobials. The EOs with the highest antibacterial activity were those belonging to the Lamiaceae family characterized by high concentrations of thymol, carvacrol and its precursor p-cymene, together with cinnamon EO, rich in cinnamaldehyde. In terms of both MIC and MBC values, the blend composed by Thymus capitatus EO 40%, Cinnamomum zeylanicum EO 20%, Thymus serpyllum EO 20% and Satureja montana EO 20% was found to be the most effective against all the isolates. Some essential oils appear to represent, at least in vitro, a valid tool against ovine mastitis pathogens. Some blends showed a remarkable effectiveness than the single oils, highlighting a synergistic effect in relation to the phytocomplex.

scholarly journals USING ESSENTIAL OILS TO COMBAT THE THREAT OF MULTI-DRUG RESISTANT BACTERIA, PSEUDOMONAS AERUGINOSA

2016 ◽  
Vol 8 (12) ◽  
pp. 180 ◽  
Author(s):  
Jenies Grullon ◽  
James P. Mack ◽  
Albert Rojtman
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Essential Oils ◽  
Alternative Treatment ◽  
Scientific Discovery ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Human Beings ◽  
Disk Diffusion Method ◽  
Cinnamon Bark

<div class="WordSection1"><p><strong>Objective: </strong>The development of antibiotics was a revolutionary scientific discovery and medical advancement that greatly extended the life expectancy of mankind. Through less than 100 y of using antibiotics to treat infectious bacteria, some of these highly adaptive organisms have developed resistance to the drugs. The healthcare field is greatly concerned with the threat of many common infections that have been considered treatable for decades, regaining its ability to be severely fatal; thus, making alternative treatments currently in high demand. This study concentrated on investigating an alternative treatment for a specific gram-negative bacterium, <em>Pseudomonas aeruginosa (P. aeruginosa)</em>, a resistance-gaining bacteria that commonly infects hospitalized patients with weakened immune systems and/or open wounds.</p><p><strong>Methods: </strong>Prior to the age of modern medicine, human beings relied on nature for medicinal treatments. In our research, we focused on determining the <em>in vitro </em>efficacy of using the essential oils, cassia and cinnamon bark, their major component, cinnamaldehyde, as well as the major component of manuka honey, methylglyoxal, as an alternative treatment against <em>P. aeruginosa</em> We tested cassia, cinnamon bark, cinnamaldehyde, and methylglyoxal using the Kirby-Bauer disk diffusion method; the diameter of the zone of inhibition for each treated bacterial sample was measured and compared with the standard antibiotic treatments, tobramycin, and amikacin.</p><p><strong>Results: </strong>This study showed that the selected essential oils, cinnamaldehyde, and methylglyoxal were as effective or better in inhibiting the growth of <em>P. aeruginosa </em>compared to the standard aminoglycoside antibiotics.</p><p><strong>Conclusion: </strong>The tested essential oils, cinnamaldehyde, and methylglyoxal may be useful as an alternative treatment for infections caused by <em>P. aeruginosa</em> and may also provide communities where antibiotics are not readily available, a cost-effective way to treat this infectious disease.</p></div>

scholarly journals In Vitro Efficacy of Essential Oils from Melaleuca Alternifolia and Rosmarinus Officinalis, Manuka Honey-based Gel, and Propolis as Antibacterial Agents Against Canine Staphylococcus Pseudintermedius Strains

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 344
Author(s):  
Gabriele Meroni ◽  
Elena Cardin ◽  
Charlotte Rendina ◽  
Valentina Rafaela Herrera Millar ◽  
Joel Fernando Soares Filipe ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antibacterial Properties ◽  
Rosmarinus Officinalis ◽  
Diffusion Method ◽  
Melaleuca Alternifolia ◽  
Disk Diffusion Method ◽  
Staphylococcus Pseudintermedius ◽  
Tea Tree

Essential oils (EOs) and honeybee products (e.g., honey and propolis) are natural mixtures of different volatile compounds that are frequently used in traditional medicine and for pathogen eradication. The aim of this study was to evaluate the antibacterial properties of tea tree (Melaleuca alternifolia) EO (TTEO), Rosmarinus officinalis EO (ROEO), manuka-based gel, and propolis against 23 strains of Staphylococcus pseudintermedius (SP) isolated from canine pyoderma. Antimicrobial resistance screening was assessed using a panel of nine antimicrobial agents coupled with a PCR approach. An aromatogram was done for both EOs, using the disk diffusion method. The minimum inhibitory concentration (MIC) was determined for all the compounds. Among the 23 SP strains, 14 (60.9%) were multidrug-resistant (MDR), 11 strains (47.8%) were methicillin-resistant (MRSP), and 9 (39.1%) were non-MDR. The mean diameter of the inhibition zone for Melaleuca and Rosmarinus were 24.5 ± 8.8 mm and 15.2 ± 8.9 mm, respectively, resulting as statistically different (p = 0.0006). MIC values of TTEO and ROEO were similar (7.6 ± 3.2% and 8.9 ± 2.1%, respectively) and no statistical significances were found. Honeybee products showed lower MIC compared to those of EOs, 0.22 ± 0.1% for Manuka and 0.8 ± 0.5% for propolis. These findings reveal a significant antibacterial effect for all the tested products.

scholarly journals Chemical composition and antimicrobial activity of Myrtus communis essential oils from Algeria

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
YACINE MOHAMADI ◽  
TAKIA LOGRADA ◽  
Messaoud Ramdani ◽  
GILLES FIGUEREDO ◽  
PIERRE CHALARD
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Bacterial Species ◽  
Antibacterial Activities ◽  
Geranyl Acetate ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Myrtus Communis ◽  
Disk Diffusion Method

Abstract. Mohamadi Y, Lograda T, Ramdani M, Figueredo G, Chalard P. 2021. Chemical composition and antimicrobial activity of Myrtus communis essential oils from Algeria. Biodiversitas 22: 933-946.  The objectives of this study were to determine the chemical composition and the antibacterial activities of Myrtus communis essential oils. The aerial parts of the myrtle were collected from nineteen localities of the Algerian coast, and subjected to hydrodistillation. The chemical composition of essential oils was analyzed using gas chromatography coupled with mass spectrometry (GC/MS). The antibacterial activity was evaluated by the disk diffusion method against ten bacterial species. Essential oils of M. communis was pale yellow oils, very fragrant, with an average yield of 0.68 ± 0.6%. Forty-six compounds were identified, representing an average of 99.17 ± 0.6% of total oils. The α-pinene was the most abundant component in the essential oils of all populations studied, with a mean of 50.1 ± 10.3%, followed by 1,8-cineole (22.27 ± 4.32%), limonene (5.16 ± 2.67%), linalool (3.79 ± 2.97%), α-terpineol (2.75 ± 1.12%), geranyl acetate (1.95 ± 0.7%) and methyl eugenol (1.40 ± 0.98%). Myrtenyl acetate was only observed in four populations with significant levels (from 10.66 to 20.94%). Six chemotypes have been identified in these oils. The α-pinene – 1,8-cineole - limonene chemotype was found in the majority of populations. The chemotype of α-pinene - 1,8-cineole - α-terphenyl acetate characterizes the Oued El-Aneb population. The α-pinene - neryl formate - limonene chemotype is observed in the Honaïn population. The remaining populations are characterized by myrtenyl acetate. The essential oils of M. communis were effective against Staphylococcus aureus ATCC 25923, Salmonella enterica ATCC35659, Proteus mirabilis, and Escherichia coli ATCC 25922; however, no significant effect was observed on Klebsiella pneumonia ATCC 700603 and Serratia liquefaciens.

scholarly journals The influence of plant essential oils on in vitro growth of Pectobacterium and Dickeya spp. bacteria

2021 ◽  
Vol 20 (6) ◽  
pp. 19-29
Author(s):  
Małgorzata Schollenberger ◽  
Agnieszka Gadomska-Gajadhur ◽  
Ewa Mirzwa-Mróz ◽  
Damian Kret ◽  
Ewa Skutnik ◽  
...  
Keyword(s):  
Essential Oils ◽  
Soft Rot ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Juniperus Virginiana ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
First Report ◽  
Plant Essential Oils

 The activity of essential oils from Eucalyptus globulus, Pinus silvestris, Lavandula angustifolia, Juniperus virginiana, Rosmarinus officinalis and Citrus paradise against the soft-rot pathogens Pectobacterium carotovorum subsp. carotovorum, Pectobacterium atrosepticum, Pectobacterium parmentieri and Dickeya solani was determined in vitro. The antibacterial activity of the essential oils will be evaluated using the disk-diffusion method by Kirby-Bauer [Bauer et al. 1966]. It was found that all the presented essential oils varied in antimicrobial activity against the four bacterial strains. No differences in the influence of streptomycin on inhibition of growth of the four bacterial strains were observed. Among six tested plants, essential oils from P. sylvestris had the strongest inhibitory effect on the growth of soft rot bacteria from Pectobacterium genus. This paper constitute the first report on the activity of the essential oils obtained from J. virginiana against soft rot bacteria. They are also the first report on the activity of the essential oils obtained from E. globulus, P. silvestris, L. angustifolia and C. paradisi against P. atrosepticum, P. parmentieri and D. solani as well as on the activity of the R. officinalis essential oils against P. atrosepticum and P. parmentieri.

scholarly journals Antifungal activity of some vegetal extracts on Candida biofilms developed on inert substratum

2011 ◽  
Vol 1 (1) ◽  
pp. 015-023 ◽  
Keyword(s):  
Essential Oils ◽  
Virulence Factors ◽  
Antifungal Drugs ◽  
Test Method ◽  
Diffusion Method ◽  
Time Curve ◽  
Disk Diffusion Method ◽  
Variable Intensity ◽  
Plant Materials ◽  
Coordinated Expression

Essential oils extracted from Citrus maxima (epicarp), Anethum graveolens (fruits) and Picea abies (leaves) were investigated for their ability to inhibit the growth and coordinated expression of virulence factors in Candida sp. strains recently isolated from clinical specimens, identified by using Vitek II automatic system and tested for susceptibility to antifungal drugs by E-test method. Plant materials were hydrodistiled in a Neo Clevenger type apparatus. Qualitative screening was performed using killing time curve and an adapted disk diffusion method. The microtiter method was used for establishing the influence of the essential oils on the microbial adherence to inert substrata. Subinhibitory concentrations of the tested essential oils were studied for their interference with the expression of soluble enzymatic virulence factors. All extracts exhibited an inhibitory activity of variable intensity on the microbial growth of planktonic as well as adherent strains, demonstrating the potential effect of the active compounds on the coordinated expression of virulence factors and consequently, on the initiation and progression of a fungal biofilm associated infection.

Chemical Composition and Antimicrobial Activity of the Leaf and Twig Essential Oils of Magnolia hypolampra Growing in Na Hang Nature Reserve, Tuyen Quang Province of Vietnam

2019 ◽  
Vol 14 (6) ◽  
pp. 1934578X1986037
Author(s):  
Chu T.T. Ha ◽  
Tran H. Thai ◽  
Nguyen T. Hien ◽  
Ha T.V. Anh ◽  
Le N. Diep ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Essential Oils ◽  
Nature Reserve ◽  
Inhibitory Concentration ◽  
Dry Weight ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Test Microorganism

The leaf and twig essential oils of Magnolia hypolampra, growing wild in Na Hang Nature Reserve, Tuyen Quang province of Vietnam, were obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The oil yield calculated on a dry weight basis from leaves of M. hypolampra was very high (1.62%, v/w), while that from twigs was much lower (0.07%, v/w). The essential oils were dominated by monoterpenoids (74.3% and 84.8%) and sesquiterpenoids (24.4% and 13.3%) with β-pinene (36.5% and 41.3%), α-pinene (23.7% and 24.4%), and germacrene D (14.6% and 5.8%) as respective major components. Antibiotic activity of the essential oil samples was tested against Gram-positive bacteria Staphylococcus aureus, Gram-negative bacteria Escherichia coli, and yeast Candida albicans using an agar disk diffusion method. Both the leaf and twig oils showed strong inhibition against all 3 tested microorganism strains with inhibition zones from 18.5 to 30.5 mm and from 45.5 to 46 mm, respectively. Minimum inhibitory concentration of the essential oils was determined using microdilution broth susceptibility assay against 7 test microorganism strains including Bacillus subtilis, Lactobacillus fermentum, Salmonella enterica, Pseudomonas aeruginosa, and 3 abovementioned strains. Minimum inhibitory concentration values of the essential oil from the twigs were from 2.0 to 8.2 mg/mL, while those from the leaves were from 4.1 to 16.4 mg/mL.

scholarly journals EFEKTIVITAS ANTIJAMUR KOMBINASI KETOKONAZOL DENGAN MINYAK ATSIRI SEREH WANGI (Cymbopogon nardus (L.) Rendle)

2017 ◽  
Vol 7 (2) ◽  
Author(s):  
Nilda Lely ◽  
Reni Indah Pratiwi ◽  
Yunita Listiani Imanda Listiani Imanda
Keyword(s):  
Essential Oils ◽  
Fungal Pathogens ◽  
Steam Distillation ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Microsporum Canis ◽  
Disk Diffusion Method ◽  
Epidermophyton Floccosum ◽  
Citronella Oil ◽  
Cymbopogon Nardus

AbstrakMinyak atsiri merupakan salah satu senyawa kimia dalam tanaman yang terbukti berpotensi sebagai antijamur. Beberapa penelitian menunjukkan bahwa minyak atsiri sereh wangi (Cymbopogon nardus L. Rendle) mempunyai aktivitas terhadap jamur patogen Tricophyton rubrum, Microsporum canis, dan Epidermophyton floccosum yang dapat menyebabkan penyakit dermatofitosis. Antijamur yang digunakan untuk pengobatan dermatofitosis ini, salah satunya adalah ketokonazol yang merupakan antijamur golongan imidazol. Pada penelitian ini ditentukan efektivitas antijamur kombinasi ketokonazol dengan minyak atsiri sereh wangi terhadap jamur penyebab dermatofitosis. Ekstraksi minyak atsiri menggunakan metode destilasi uap air. Uji efektivitas antijamur kombinasi ketokonazol dengan minyak atsiriri sereh wangi dengan metode difusi agar. Hasil penelitian kombinasi ketokonazol dengan minyak atsiri menunjukkan efek sinergis, karena diameter zona hambat ketokonazol mengalami peningkatan setelah dikombinasikan dengan minyak atsiri. Diameter zona hambat ketokonazol tunggal Tricophyton rubrum ATCC 28188 13,26 mm setelah dikombinasikan menjadi 24,9 mm, Microsporum canis ATCC 32699 12,8 mm setelah dikombinasikan menjadi 22,56 mm, Epidermophyton floccosum ATCC 52066 12,76 mm setelah dikombinasikan menjadi 19,5 mm. Hal ini disebabkan karena mekanisme kerja yang sinergis dari minyak atsiri sereh wangi (Cymbopogon nardus L. Rendle)  dan ketokonazol.Kata kunci: citronella, dermatofitosis, sinergisAbstractEssential oil is one of the chemical compounds in plants that have been proven potential as an antifungal agent. Some studies showed that the essential oil of citronella (Cymbopogon nardus L. Rendle) could resist fungal pathogens Tricophyton rubrum, Microsporum canis and Epidermophyton floccosum, which cause dermatophytosis. Antifungals used for the treatment of dermatophytosis is ketoconazole, which belongs to imidazole group. The purpose of this study was to determine the effectiveness of antifungal ketoconazole in combination with essential oils of citronella against dermatophytosis. Extraction of essential oils was carried out using steam distillation. The effectiveness of antifungal ketoconazole in combination with citronella oil was measured using disk diffusion method. The result of the combination of ketoconazole with essential oils was synergistic as proven by the increased diameter of inhibition zone ketoconazole after combined with essential oils. Ketoconazole inhibition zone diameter single Tricophyton rubrum ATCC 28188 13.26 mm after combined into a 24.9 mm, Microsporum canis ATCC 32699 12.8 mm after combined into a 22.56 mm, Epidermophyton floccosum ATCC 52066 12.76 mm after combined into 19, 5 mm. This is because the synergitic mechanism of action of essential oils of citronella (Cymbopogon nardus L. Rendle) and ketoconazole.Key words: citronella, dermatophytosis, synergistic

scholarly journals Antibacterial activity of aromatic plants essential oils from Serbia against the Listeria monocytogenes

2009 ◽  
Vol 54 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Anita Klaus ◽  
Damir Beatovic ◽  
Miomir Niksic ◽  
Slavica Jelacic ◽  
Tanja Petrovic
Keyword(s):  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Disk Diffusion Method ◽  
Growth And Survival ◽  
Salvia Officinalis L ◽  
Rosmarinus Officinalis L

The purpose of this study was to examine the effectiveness of selected essential oils for the control of the growth and survival of pathogenic bacteria Listeria monocytogenes ATCC 19112 and Listeria monocytogenes ATCC 19115, which are of significant importance in food hygiene. Essential oils extracted from Salvia officinalis L., Rosmarinus officinalis L., Majorana hortensis Moench., Thymus vulgaris L., Carum carvi L., Pimpinella anisum L. and Coriandrum sativum L. were evaluated. Antibacterial activity was done by the disk diffusion method in the presence of pure essential oils and four suspensions in alcohol. The best results obtained with Thymus vulgaris and Majorana hortensis essential oils, which were acting microbicidaly on both observed strains of Listeria monocytogenes, even in the small concentration. Because some of the essential oils were highly inhibitory even in small quantities to selected pathogenic bacteria, they may provide alternatives to conventional antimicrobial additives in foods. .

scholarly journals The composition and antibacterial activity of the essential oil of Levisticum officinale Koch. flowers and fruits at different developmental stages

2010 ◽  
Vol 75 (12) ◽  
pp. 1661-1669 ◽  
Author(s):  
Mohammad Mirjalili ◽  
Peyman Salehi ◽  
Ali Sonboli ◽  
Javad Hadian ◽  
Samad Ebrahimi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Developmental Stages ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Mature Fruit ◽  
Disk Diffusion Method ◽  
Immature Fruit

The composition and antibacterial activity of the essential oil of Levisticum officinale Koch. at different developmental stages (flower, immature fruit, green mature fruit and ripened fruit) is reported. The essential oils were obtained by hydrodistillation of air-dried samples and their antibacterial activities were tested against seven bacteria. The yield of oil (w/w %) in different stages was in the order: immature fruit (1.5 %) > green mature fruit (1.0 %) > ripened fruit (0.6 %) > flower (0.1 %). The essential oils were analyzed by GC and GC-MS. In total, 27, 31, 28 and 26 constituents were identified and quantified in the mentioned samples, respectively. Monoterpene hydrocarbons were the main group of compounds in the green mature fruit (79.2 %), immature fruit (78.4 %), ripened fruit (75.2 %) and flower (44.0 %). The antibacterial activity of the oils was evaluated by the disk diffusion method using M?ller-Hinton agar and determination of inhibition zones. The results of the bioassays showed some variations between the three tested oils in their inhibitory activity against the tested bacteria at a 10 ?l disc-1 concentration. The oils from mature and ripened fruit exhibited potent antibacterial activity against Bacillus subtilis, with minimum inhibitory concentration (MIC) values of 0.90 mg ml-1 in mature and ripen fruits.

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