scholarly journals Transcriptomic and Metabolic Profiling of High-Temperature Treated Storage Roots Reveals the Mechanism of Saccharification in Sweetpotato (Ipomoea batatas (L.) Lam.)

2021 ◽  
Vol 22 (13) ◽  
pp. 6641
Author(s):  
Chen Li ◽  
Meng Kou ◽  
Mohamed Hamed Arisha ◽  
Wei Tang ◽  
Meng Ma ◽  
...  
Keyword(s):  
High Temperature ◽  
Metabolic Pathways ◽  
Transcriptome Sequencing ◽  
Ipomoea Batatas ◽  
Sequencing Analysis ◽  
Storage Roots ◽  
Metabolic Analysis ◽  
Cooking Process

The saccharification of sweetpotato storage roots is a common phenomenon in the cooking process, which determines the edible quality of table use sweetpotato. In the present study, two high saccharified sweetpotato cultivars (Y25, Z13) and one low saccharified cultivar (X27) in two growth periods (S1, S2) were selected as materials to reveal the molecular mechanism of sweetpotato saccharification treated at high temperature by transcriptome sequencing and non-targeted metabolome determination. The results showed that the comprehensive taste score, sweetness, maltose content and starch change of X27 after steaming were significantly lower than those of Y25 and Z13. Through transcriptome sequencing analysis, 1918 and 1520 differentially expressed genes were obtained in the two periods of S1 and S2, respectively. Some saccharification-related transcription factors including MYB families, WRKY families, bHLH families and inhibitors were screened. Metabolic analysis showed that 162 differentially abundant metabolites related to carbohydrate metabolism were significantly enriched in starch and sucrose capitalization pathways. The correlation analysis between transcriptome and metabolome confirmed that the starch and sucrose metabolic pathways were significantly co-annotated, indicating that it is a vitally important metabolic pathway in the process of sweetpotato saccharification. The data obtained in this study can provide valuable resources for follow-up research on sweetpotato saccharification and will provide new insights and theoretical basis for table use sweetpotato breeding in the future.

scholarly journals Ginkgo biloba L. Responds to Red and Blue Light: Via Phenylpropanoid and Flavonoid Biosynthesis Pathway

Forests ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 1079
Author(s):  
Lei Zhang ◽  
Gaiping Wang ◽  
Guibin Wang ◽  
Fuliang Cao
Keyword(s):  
Blue Light ◽  
Ginkgo Biloba ◽  
Metabolic Pathways ◽  
Transcriptome Sequencing ◽  
Molecular Mechanisms ◽  
Light Quality ◽  
Red Light ◽  
Enrichment Analysis ◽  
Flavonoid Biosynthesis ◽  
Sequencing Analysis

Light quality is a key environmental factor affecting plant growth and development. In this study, RNA-seq technology was used to explore the molecular mechanisms of ginkgo metabolism under different monochromatic lights. Leaves were used for transcriptome sequencing analysis after being irradiated by red, blue, and white LED lights. After treatment, 2040 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) analysis showed that the DEGs were annotated into 49 terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that 736 DEGs were enriched in 100 metabolic pathways, and 13 metabolic pathways were significantly enriched, especially ‘phenylpropanoid biosynthesis’ and ‘flavonoid biosynthesis’. Further analysis of DEGs expression in the two pathways showed that Ginkgo biloba adapts to blue light mainly by promoting the expression of GbFLS to synthesize quercetin, kaempferol, and myncetin, and adapts to red light by promoting the expression of GbDFR to synthesize leucocyanidin. Nine DEGs were randomly selected for qRT-PCR verification, and the gene expression results were consistent with that of transcriptome sequencing. In conclusion, this study is the first to explore the molecular mechanism of ginkgo in response to different monochromatic lights, and it will lay a foundation for the research and application of light quality in the cultivation of leaf-use G. biloba.

scholarly journals Black Polyethylene Tunnel Covers Affect Plant Production and Quality of Sweetpotato Transplants

HortScience ◽  
2000 ◽  
Vol 35 (2) ◽  
pp. 202-204
Author(s):  
D.R. La Bonte ◽  
A.Q. Villordon ◽  
J.R. Schultheis ◽  
D.W. Monks
Keyword(s):  
North Carolina ◽  
Ipomoea Batatas ◽  
Growing Season ◽  
Plant Production ◽  
Ground Control ◽  
Bare Ground ◽  
Storage Roots ◽  
Early Season ◽  
Late Season

The influence of a black polyethylene tunnel cover (BTC) was evaluated for its effect on quantity and quality of sweetpotato [Ipomoea batatas (L.) Lam.] transplants in plant beds in Louisiana and North Carolina. Use of BTC increased production of `Beauregard' transplants from 63% to 553% in comparison with the bare ground control. `Jewel' was less responsive; BTC treatments increased transplant production by at least 48% in Louisiana over the bare ground control, but no increase was observed in North Carolina. Individual transplant weight was at least 34% less in BTC treatments than in the control. The first harvest of cuttings in BTC beds was at least 14 days prior to that in control beds. Transplant quality was assessed as yield of storage roots in repeated trials that extended throughout the normal growing season. Yield of storage roots was not affected by BTC in early season plantings, but was frequently lower for BTC treatment transplants in middle and late season plantings. We therefore do not recommend this method as a means of increasing sweetpotato plant production from bedded roots.

scholarly journals Novel Functional Genes Involved in Transdifferentiation of Canine ADMSCs Into Insulin-Producing Cells, as Determined by Absolute Quantitative Transcriptome Sequencing Analysis

2021 ◽  
Vol 9 ◽  
Author(s):  
Pengxiu Dai ◽  
Jiakai Li ◽  
Yijing Chen ◽  
Luwen Zhang ◽  
Xinke Zhang ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
Transcriptome Sequencing ◽  
Enrichment Analysis ◽  
Sequencing Analysis ◽  
Insulin Producing Cells ◽  
High Maturity ◽  
Potential Resource ◽  
Treatment Of Diabetes ◽  
Upregulated Genes ◽  
Future Work

The transdifferentiation of adipose-derived mesenchymal stem cells (ADMSCs) into insulin-producing cells (IPCs) is a potential resource for the treatment of diabetes. However, the changes of genes and metabolic pathways on the transdifferentiation of ADMSCs into IPCs are largely unknown. In this study, the transdifferentiation of canine ADMSCs into IPCs was completed using five types of procedures. Absolute Quantitative Transcriptome Sequencing Analysis was performed at different stages of the optimal procedure. A total of 60,151 transcripts were obtained. Differentially expressed genes (DEGs) were divided into five groups: IPC1 vs. ADSC (1169 upregulated genes and 1377 downregulated genes), IPC2 vs. IPC1 (1323 upregulated genes and 803 downregulated genes), IPC3 vs. IPC2 (722 upregulated genes and 680 downregulated genes), IPC4 vs. IPC3 (539 upregulated genes and 1561 downregulated genes), and Beta_cell vs. IPC4 (2816 upregulated genes and 4571 downregulated genes). The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs revealed that many genes and signaling pathways that are essential for transdifferentiation. Hnf1B, Dll1, Pbx1, Rfx3, and Foxa1 were screened out, and the functions of five genes were verified further by overexpression and silence. Foxa1, Pbx1, and Rfx3 exhibited significant effects, can be used as specific key regulatory factors in the transdifferentiation of ADMSCs into IPCs. This study provides a foundation for future work to understand the mechanisms of the transdifferentiation of ADMSCs into IPCs and acquire IPCs with high maturity.

scholarly journals Performance and Quality of Sweetpotato Cultivars Grown in Quebec, Canada with Biodegradable Plastic Mulch

2015 ◽  
Vol 25 (6) ◽  
pp. 815-823 ◽  
Author(s):  
David Wees ◽  
Philippe Seguin ◽  
Josée Boisclair ◽  
Chloé Gendre
Keyword(s):  
Ipomoea Batatas ◽  
Soft Rot ◽  
Soluble Solids ◽  
Storage Roots ◽  
Plastic Mulch ◽  
Selected Lines ◽  
Eastern Canada ◽  
Black Plastic ◽  
High Yields

There is an increased interest in producing sweetpotato (Ipomoea batatas) in parts of eastern Canada, which are farther north and have fewer growing degree days (GDD) than traditional production regions in the southern United States. There is currently little information on cultivar selection for farmers in these northern regions. We evaluated yields and quality of 15 sweetpotato cultivars and selected lines grown with black plastic mulch but without pesticides. The best marketable yields (18–25 t·ha−1) in this trial were comparable to average marketable yields obtained in traditional sweetpotato-producing regions. Of the orange-fleshed cultivars and selected lines, Beauregard, B94-24, and Evangeline had high yields and warrant further testing. Covington, a common cultivar in North Carolina and Ontario, had poor yields in our conditions: it may require more GDD. ‘Porto Rico’ and ‘Ginseng Red’ had poor yields mainly because their storage roots were too small. The yellow-fleshed ‘Georgia Jet’ and GJ2010 had very vigorous vines and produced high yields, but had a high culling rate because of malformed or cracked storage roots. The white-fleshed ‘Murasaki-29’, ‘O’Henry’, and ‘Japanese’ also had high yields; whereas ‘White Travis’ and ‘Korean Purple’ had poor yields with small storage roots. Establishing the crop with rooted transplants instead of slips was satisfactory as long as the transplants were less than 4 weeks old. On the basis of our limited data, the following cultivars and selected lines may be suitable for Quebec, Canada: Beauregard, B94-24, Evangeline, Georgia Jet, GJ2010, and Murasaki-29. ‘Evangeline’ and ‘Covington’ had higher soluble solid contents than the other cultivars. High percent soluble solids may be desired by consumers. In spite of the absence of pesticides, very few pest or disease problems were observed except for some postharvest soft rot.

Cathartic Effect of Suicide Attempts Not Limited to Depression

Crisis ◽  
2003 ◽  
Vol 24 (2) ◽  
pp. 73-78 ◽  
Author(s):  
Yves Sarfati ◽  
Blandine Bouchaud ◽  
Marie-Christine Hardy-Baylé
Keyword(s):  
Quality Of Life ◽  
Depressive Symptoms ◽  
Personality Traits ◽  
Rating Scales ◽  
Suicide Attempts ◽  
World Health ◽  
Life Questionnaire ◽  
Short Term

Summary: The cathartic effect of suicide is traditionally defined as the existence of a rapid, significant, and spontaneous decrease in the depressive symptoms of suicide attempters after the act. This study was designed to investigate short-term variations, following a suicide attempt by self-poisoning, of a number of other variables identified as suicidal risk factors: hopelessness, impulsivity, personality traits, and quality of life. Patients hospitalized less than 24 hours after a deliberate (moderate) overdose were presented with the Montgomery-Asberg Depression and Impulsivity Rating Scales, Hopelessness scale, MMPI and World Health Organization's Quality of Life questionnaire (abbreviated versions). They were also asked to complete the same scales and questionnaires 8 days after discharge. The study involved 39 patients, the average interval between initial and follow-up assessment being 13.5 days. All the scores improved significantly, with the exception of quality of life and three out of the eight personality traits. This finding emphasizes the fact that improvement is not limited to depressive symptoms and enables us to identify the relative importance of each studied variable as a risk factor for attempted suicide. The limitations of the study are discussed as well as in particular the nongeneralizability of the sample and setting.

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