scholarly journals Molecule-Resolved Visualization of Particulate Matter on Human Skin Using Multimodal Nonlinear Optical Imaging

2021 ◽  
Vol 22 (10) ◽  
pp. 5199
Author(s):  
Eun-Soo Lee ◽  
Suho Kim ◽  
Sang-Won Lee ◽  
Jinsang Jung ◽  
Sung Hoon Lee ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Human Skin ◽  
Nonlinear Optical ◽  
Skin Diseases ◽  
Imaging System ◽  
Ex Vivo ◽  
Skin Barrier ◽  
Tape Stripping ◽  
Nonlinear Optical Imaging ◽  
Different Sources

Precise measurement of particulate matter (PM) on skin is important for managing and preventing PM-related skin diseases. This study aims to directly visualize the deposition and penetration of PM into human skin using a multimodal nonlinear optical (MNLO) imaging system. We successfully obtained PM particle signals by merging two different sources, C–C vibrational frequency and autofluorescence, while simultaneously visualizing the anatomical features of the skin via keratin, collagen, and elastin. As a result, we found morphologically dependent PM deposition, as well as increased deposition following disruption of the skin barrier via tape-stripping. Furthermore, PM penetrated more and deeper into the skin with an increase in the number of tape-strippings, causing a significant increase in the secretion of pro-inflammatory cytokines. Our results suggest that MNLO imaging could be a useful technique for visualizing and quantifying the spatial distribution of PM in ex vivo human skin tissues.

scholarly journals Multimode nonlinear optical imaging of the dermis in ex vivo human skin based on the combination of multichannel mode and Lambda mode

2006 ◽  
Vol 14 (17) ◽  
pp. 7810 ◽  
Author(s):  
Shuangmu Zhuo ◽  
Jianxin Chen ◽  
Tianshu Luo ◽  
Dingsong Zou ◽  
Jingjun Zhao
Keyword(s):  
Optical Imaging ◽  
Human Skin ◽  
Nonlinear Optical ◽  
Ex Vivo ◽  
Nonlinear Optical Imaging

scholarly journals Compromising human skin in vivo and ex vivo to study skin barrier repair

2019 ◽  
Vol 1864 (8) ◽  
pp. 1103-1108 ◽  
Author(s):  
T. Berkers ◽  
W.A. Boiten ◽  
S. Absalah ◽  
J. van Smeden ◽  
A.P.M. Lavrijsen ◽  
...  
Keyword(s):  
Human Skin ◽  
Ex Vivo ◽  
Skin Barrier ◽  
Barrier Repair ◽  
Skin Barrier Repair

scholarly journals Effects of Particulate Matter on Healthy Skin: A Comparative Study Between High and Low-particulate Matter Periods

2020 ◽  
Author(s):  
Se Jin Oh ◽  
Dokyoung Yoon ◽  
Ji-Hye Park ◽  
Jong Hee Lee
Keyword(s):  
Particulate Matter ◽  
Human Skin ◽  
Skin Diseases ◽  
Airborne Particulate Matter ◽  
Skin Tone ◽  
Transepidermal Water Loss ◽  
Biophysical Parameters ◽  
Healthy Human ◽  
Facial Analysis ◽  
Analysis System

Abstract Background The influence of airborne particulate matter (PM) on skin has primarily been studied in patients with skin diseases such as atopic dermatitis. Recently, the effect of PM on healthy human skin has gained attention. This study aimed to evaluate the relationship between PM concentration and objective skin changes in healthy subjects. Methods This prospective study enrolled 25 healthy volunteers without any skin disease. Data regarding daily meteorological parameters and air pollution were collected during a high-PM period and a low-PM period for 14 days. Environmental and lifestyle factors that might influence skin conditions of subjects were also collected during the study period. Biophysical parameters of the skin such as transepidermal water loss (TEWL), hydration, erythema index, and melanin index were measured. Pores, wrinkles, sebum, and skin tone were evaluated using a facial analysis system. Results Mean TEWL value during the high-PM period was significantly higher than that during the low-PM period (10.16 g/m2/h vs. 5.99 g/m2/h, p = 0.0005). Mean erythema index was significantly higher in the high-PM period than that in the low-PM period (4.3 vs. 3.42, p = 0.038). For facial analysis system indices, uniformity of skin tone was higher in the low-PM period than that in the high-PM period (p < 0.0001). In addition, with increasing PM10 and PM2.5, TEWL also showed increase when other environmental components were constant [regression coefficient (RC) = 0.1529, p < 0.0001 for PM10; RC = 0.2055, p = 0.0153 for PM2.5]. Conclusions Increased PM concentrations may contribute to disturbed barrier function, increased facial erythema, and uneven skin tone even in healthy human skin.

scholarly journals Multipurpose nonlinear optical imaging system forin vivoandex vivomultimodal histology

2015 ◽  
Vol 2 (1) ◽  
pp. 016003 ◽  
Author(s):  
Martin Weinigel ◽  
Hans Georg Breunig ◽  
Aisada Uchugonova ◽  
Karsten König
Keyword(s):  
Optical Imaging ◽  
Nonlinear Optical ◽  
Imaging System ◽  
Nonlinear Optical Imaging ◽  
Optical Imaging System

scholarly journals Detection of Antimicrobial Peptides in Stratum Corneum by Mass Spectrometry

2021 ◽  
Vol 22 (8) ◽  
pp. 4233
Author(s):  
Adrienn Jenei ◽  
Gergő Kalló ◽  
Zsolt Dajnoki ◽  
Krisztián Gáspár ◽  
Andrea Szegedi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Stratum Corneum ◽  
Skin Diseases ◽  
Skin Barrier ◽  
Tape Stripping ◽  
Reaction Monitoring ◽  
Sample Collection ◽  
Novel Approach ◽  
Parallel Reaction Monitoring ◽  
Immunomodulatory Peptides

Antimicrobial and immunomodulatory peptides (AMPs) are considered as the key players in the maintenance of skin barrier functions. Here, we developed a novel approach for the examination of AMPs in the outermost layer of the epidermis, namely stratum corneum (SC). The SC sample collection by tape stripping was coupled with detection by highly specific and sensitive parallel reaction monitoring (PRM)-based mass spectrometry. We found that hexane-free processing of SC samples produced higher protein yield compared to hexane-based extraction. Of the 18 investigated peptides, 9 could be detected either in healthy or in inflamed skin specimens. Regarding the amount of S100A8, LCN2, LACRT and LYZ significant topographical differences were described among gland poor (GP), sebaceous gland rich (SGR) and apocrine gland rich (AGR) healthy skin regions. We applied a minimally invasive, reproducible approach for sampling, which can be assessed for research and diagnostic purposes and for monitoring the effectiveness of therapies in skin diseases.

scholarly journals Spermidine-induced recovery of human dermal structure and barrier function by skin microbiome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Gihyeon Kim ◽  
Misun Kim ◽  
Minji Kim ◽  
Changho Park ◽  
Youngmin Yoon ◽  
...  
Keyword(s):  
Human Skin ◽  
Barrier Function ◽  
Skin Diseases ◽  
Lipid Synthesis ◽  
Skin Barrier ◽  
Streptococcus Thermophilus ◽  
Skin Barrier Function ◽  
Skin Microbiome ◽  
Skin Structure

AbstractAn unbalanced microbial ecosystem on the human skin is closely related to skin diseases and has been associated with inflammation and immune responses. However, little is known about the role of the skin microbiome on skin aging. Here, we report that the Streptococcus species improved the skin structure and barrier function, thereby contributing to anti-aging. Metagenomic analyses showed the abundance of Streptococcus in younger individuals or those having more elastic skin. Particularly, we isolated Streptococcus pneumoniae, Streptococcus infantis, and Streptococcus thermophilus from face of young individuals. Treatment with secretions of S. pneumoniae and S. infantis induced the expression of genes associated with the formation of skin structure and the skin barrier function in human skin cells. The application of culture supernatant including Streptococcal secretions on human skin showed marked improvements on skin phenotypes such as elasticity, hydration, and desquamation. Gene Ontology analysis revealed overlaps in spermidine biosynthetic and glycogen biosynthetic processes. Streptococcus-secreted spermidine contributed to the recovery of skin structure and barrier function through the upregulation of collagen and lipid synthesis in aged cells. Overall, our data suggest the role of skin microbiome into anti-aging and clinical applications.

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