scholarly journals Detection of Genomic Uracil Patterns

2021 ◽  
Vol 22 (8) ◽  
pp. 3902
Author(s):  
Angéla Békési ◽  
Eszter Holub ◽  
Hajnalka Laura Pálinkás ◽  
Beáta G. Vértessy
Keyword(s):  
Chemical Reactivity ◽  
Living Cells ◽  
Critical Assessment ◽  
Detection Methods ◽  
Special Focus ◽  
Cytosine Deamination ◽  
Genome Wide ◽  
Recent Developments ◽  
In Situ Detection

The appearance of uracil in the deoxyuridine moiety of DNA is among the most frequently occurring genomic modifications. Three different routes can result in genomic uracil, two of which do not require specific enzymes: spontaneous cytosine deamination due to the inherent chemical reactivity of living cells, and thymine-replacing incorporation upon nucleotide pool imbalances. There is also an enzymatic pathway of cytosine deamination with multiple DNA (cytosine) deaminases involved in this process. In order to describe potential roles of genomic uracil, it is of key importance to utilize efficient uracil-DNA detection methods. In this review, we provide a comprehensive and critical assessment of currently available uracil detection methods with special focus on genome-wide mapping solutions. Recent developments in PCR-based and in situ detection as well as the quantitation of genomic uracil are also discussed.

scholarly journals Real Time In-situ Detection System Research for Methane in Offshore Shallow Gas based on Thin Film Interface

2018 ◽  
Vol 232 ◽  
pp. 04053
Author(s):  
Cheng-xing Miao ◽  
Qing Li ◽  
Sheng-yao Jia
Keyword(s):  
Real Time ◽  
Detection Efficiency ◽  
Detection System ◽  
Detection Methods ◽  
Shallow Gas ◽  
Micro Gas Sensor ◽  
Detection Probe ◽  
On Line ◽  
In Situ Detection

In order to get ridded of the non real-time detection methods of artificial site sampled and laboratory instrument analyzed in the field of methane detection in the offshore shallow gas, real-time in-situ detection system for methane in offshore shallow gas was designed by the film interface.The methane in the offshore shallow gas through the gas-liquid separation membrane of polymer permeation into the system internal detection probe, analog infrared micro gas sensor sensed the methane concentration and the corresponded output value, data acquisition and communication node fitted into standard gas concentration.Based on the experimental data compared with the traditional detection method, and further analyzed the causes of error produced by the case experiment. The application results show that the system can achieve a single borehole layout, long-term on-line in-situ on-line detection, and improve the detection efficiency and the timeliness of the detection data.

In Situ Detection of Released H2O2 from Living Cells by Carbon Cloth-Supported Graphene/Au–Pt Nanoparticles

2021 ◽  
Author(s):  
Jing Bao ◽  
Huisi Yang ◽  
Jiaying Zhao ◽  
Cheng Yang ◽  
Yu Duan ◽  
...  
Keyword(s):  
Pt Nanoparticles ◽  
Living Cells ◽  
Carbon Cloth ◽  
In Situ Detection

Biosensor based on 3D graphene-supported Fe3O4 quantum dots as biomimetic enzyme for in situ detection of H2O2 released from living cells

2017 ◽  
Vol 244 ◽  
pp. 1037-1044 ◽  
Author(s):  
Yanan Zhao ◽  
Danqun Huo ◽  
Jing Bao ◽  
Mei Yang ◽  
Mei Chen ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Living Cells ◽  
3D Graphene ◽  
In Situ Detection

Genetics of anxiety disorders

2020 ◽  
pp. 928-937
Author(s):  
Michael G. Gottschalk ◽  
Katharina Domschke
Keyword(s):  
Anxiety Disorder ◽  
Anxiety Disorders ◽  
Association Studies ◽  
Twin Studies ◽  
Behavioural Therapy ◽  
Special Focus ◽  
Behavioural Inhibition ◽  
Gene Environment ◽  
Genome Wide ◽  
Recent Developments

Genetics of anxiety disorders highlights recent developments in the identification of genetic variation associated with specific phobias, social anxiety disorder, agoraphobia, panic disorder, generalized anxiety disorder, and cross-disorder intermediate phenotypes like neuroticism/negative affectivity, introversion, behavioural inhibition, harm avoidance, and anxiety sensitivity. It reviews evidence gathered by family and twin studies, structural and rare variations, genome-wide association studies (GWAS), candidate genes, gene–environment, and epigenetic approaches. Special focus is placed upon functional hypotheses linked to the serotonergic and catecholaminergic, as well as neuropeptide and neurohormonal, signalling systems, followed by multi-level neurophysiological, neuroimaging, and behavioural investigations of anxiety- and fear-related traits. Special emphasis is put on dimensional psychopathology and biomarkers of disease course, susceptibility, and treatment response to cognitive behavioural therapy or psychopharmacological interventions. Moreover, challenges and perspectives are addressed related to drug discovery, personalized medicine, polygenic risk assessment, relevance to nosological domains, and plasticity of the underlying epigenetic architecture.

DNAzyme Based Nanomachine for in Situ Detection of MicroRNA in Living Cells

ACS Sensors ◽  
2017 ◽  
Vol 2 (12) ◽  
pp. 1847-1853 ◽  
Author(s):  
Jing Liu ◽  
Meirong Cui ◽  
Hong Zhou ◽  
Wenrong Yang
Keyword(s):  
Living Cells ◽  
In Situ Detection

Tetrahedron Probes for Ultrasensitive In Situ Detection of Telomerase and Surface Glycoprotein Activity in Living Cells

2019 ◽  
Vol 92 (2) ◽  
pp. 2310-2315 ◽  
Author(s):  
Xiao Guo ◽  
Xiaoling Wu ◽  
Maozhong Sun ◽  
Liguang Xu ◽  
Hua Kuang ◽  
...  
Keyword(s):  
Living Cells ◽  
Surface Glycoprotein ◽  
In Situ Detection
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