DNAzyme Based Nanomachine for in Situ Detection of MicroRNA in Living Cells

ACS Sensors ◽  
2017 ◽  
Vol 2 (12) ◽  
pp. 1847-1853 ◽  
Author(s):  
Jing Liu ◽  
Meirong Cui ◽  
Hong Zhou ◽  
Wenrong Yang
Keyword(s):  
Living Cells ◽  
In Situ Detection

In Situ Detection of Released H2O2 from Living Cells by Carbon Cloth-Supported Graphene/Au–Pt Nanoparticles

2021 ◽  
Author(s):  
Jing Bao ◽  
Huisi Yang ◽  
Jiaying Zhao ◽  
Cheng Yang ◽  
Yu Duan ◽  
...  
Keyword(s):  
Pt Nanoparticles ◽  
Living Cells ◽  
Carbon Cloth ◽  
In Situ Detection

Biosensor based on 3D graphene-supported Fe3O4 quantum dots as biomimetic enzyme for in situ detection of H2O2 released from living cells

2017 ◽  
Vol 244 ◽  
pp. 1037-1044 ◽  
Author(s):  
Yanan Zhao ◽  
Danqun Huo ◽  
Jing Bao ◽  
Mei Yang ◽  
Mei Chen ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Living Cells ◽  
3D Graphene ◽  
In Situ Detection

Tetrahedron Probes for Ultrasensitive In Situ Detection of Telomerase and Surface Glycoprotein Activity in Living Cells

2019 ◽  
Vol 92 (2) ◽  
pp. 2310-2315 ◽  
Author(s):  
Xiao Guo ◽  
Xiaoling Wu ◽  
Maozhong Sun ◽  
Liguang Xu ◽  
Hua Kuang ◽  
...  
Keyword(s):  
Living Cells ◽  
Surface Glycoprotein ◽  
In Situ Detection

Nature-Inspired DNA Nanosensor for Real-Time in Situ Detection of mRNA in Living Cells

ACS Nano ◽  
2015 ◽  
Vol 9 (5) ◽  
pp. 5609-5617 ◽  
Author(s):  
Chor Yong Tay ◽  
Liang Yuan ◽  
David Tai Leong
Keyword(s):  
Real Time ◽  
Living Cells ◽  
In Situ Detection

scholarly journals Detection of Genomic Uracil Patterns

2021 ◽  
Vol 22 (8) ◽  
pp. 3902
Author(s):  
Angéla Békési ◽  
Eszter Holub ◽  
Hajnalka Laura Pálinkás ◽  
Beáta G. Vértessy
Keyword(s):  
Chemical Reactivity ◽  
Living Cells ◽  
Critical Assessment ◽  
Detection Methods ◽  
Special Focus ◽  
Cytosine Deamination ◽  
Genome Wide ◽  
Recent Developments ◽  
In Situ Detection

The appearance of uracil in the deoxyuridine moiety of DNA is among the most frequently occurring genomic modifications. Three different routes can result in genomic uracil, two of which do not require specific enzymes: spontaneous cytosine deamination due to the inherent chemical reactivity of living cells, and thymine-replacing incorporation upon nucleotide pool imbalances. There is also an enzymatic pathway of cytosine deamination with multiple DNA (cytosine) deaminases involved in this process. In order to describe potential roles of genomic uracil, it is of key importance to utilize efficient uracil-DNA detection methods. In this review, we provide a comprehensive and critical assessment of currently available uracil detection methods with special focus on genome-wide mapping solutions. Recent developments in PCR-based and in situ detection as well as the quantitation of genomic uracil are also discussed.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

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