scholarly journals CRISPR/Cas Technology in Pig-to-Human Xenotransplantation Research

2021 ◽  
Vol 22 (6) ◽  
pp. 3196
Author(s):  
Natalia Ryczek ◽  
Magdalena Hryhorowicz ◽  
Joanna Zeyland ◽  
Daniel Lipiński ◽  
Ryszard Słomski
Keyword(s):  
Genetically Modified Organisms ◽  
Human Life ◽  
Genetically Modified ◽  
Phylogenetic Distance ◽  
Dna Breaks ◽  
Careful Evaluation ◽  
Engineering Research ◽  
Double Strand Dna Breaks ◽  
Immunological Reactions ◽  
Human Xenotransplantation

CRISPR/Cas (clustered regularly interspaced short palindromic repeats linked to Cas nuclease) technology has revolutionized many aspects of genetic engineering research. Thanks to it, it became possible to study the functions and mechanisms of biology with greater precision, as well as to obtain genetically modified organisms, both prokaryotic and eukaryotic. The changes introduced by the CRISPR/Cas system are based on the repair paths of the single or double strand DNA breaks that cause insertions, deletions, or precise integrations of donor DNA. These changes are crucial for many fields of science, one of which is the use of animals (pigs) as a reservoir of tissues and organs for xenotransplantation into humans. Non-genetically modified animals cannot be used to save human life and health due to acute immunological reactions resulting from the phylogenetic distance of these two species. This review is intended to collect and summarize the advantages as well as achievements of the CRISPR/Cas system in pig-to-human xenotransplantation research. In addition, it demonstrates barriers and limitations that require careful evaluation before attempting to experiment with this technology.

scholarly journals THE EUROPEAN UNION, THE UNITED STATES, AND THE GENETICALLY MODIFIED ORGANISMS (GMOs)

2020 ◽  
Vol 21 (1) ◽  
pp. 11-20
Author(s):  
Anisa ◽  
Chelsilya ◽  
Grace Yohana ◽  
Mucco Eva ◽  
Morry Zefanya ◽  
...  
Keyword(s):  
European Union ◽  
Genetically Modified Organisms ◽  
Human Life ◽  
Genetically Modified ◽  
The United States ◽  
Environmental Damage ◽  
The European Union ◽  
Technological Advances ◽  
The Us ◽  
The Eu

Current technological advances have been present in all aspects of human life, including technological advances in biotechnology. Biotechnology not only raises hope for science but also raises heated debates among scientists, especially between the European Union and the US. This debate arises because of differences in perspective between the EU and the US. The EU has stringent rules regarding the development efforts of genetically modified organisms (GMOs). At the same time, the US thinks that GMOs are part of agriculture, so there is no need for any special laws to regulate them. Various side effects also come hand in hand with the birth of GMOs. They are ranging from adverse effects on human health, the health of food products, and even environmental damage. The development of GMOs can damage the ecosystem of species that exist in the environment. Still, more complex problems arise due to GMOs like economic problems and monopolies.   Keywords: The  GMOs, The EU, The US.

scholarly journals Risks and Precautions of Genetically Modified Organisms

ISRN Ecology ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Dhan Prakash ◽  
Sonika Verma ◽  
Ranjana Bhatia ◽  
B. N. Tiwary
Keyword(s):  
Risk Assessment ◽  
Genetically Modified Organisms ◽  
Human Life ◽  
Genetically Modified ◽  
Environmental Agreements ◽  
Precautionary Approach ◽  
Biotechnological Approach ◽  
The World ◽  
Controlled Environments ◽  
Potential Risks

Commercial potential of biotechnology is immense since the scope of its activity covers the entire spectrum of human life. The most potent biotechnological approach is the transfer of specifically constructed gene assemblies through various techniques. However, this deliberate modification and the resulting entities thereof have become the bone of contention all over the world. Benefits aside, genetically modified organisms (GMOs) have always been considered a threat to environment and human health. In view of this, it has been considered necessary by biosafety regulations of individual countries to test the feasibility of GMOs in contained and controlled environments for any potential risks they may pose. This paper describes the various aspects of risk, its assessment, and management which are imperative in decision making regarding the safe use of GMOs. Efficient efforts are necessary for implementation of regulations. Importance of the risk assessment, management, and precautionary approach in environmental agreements and activism is also discussed.

scholarly journals Facts and Myths about GM Food—The Case of Poland

Agriculture ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 791
Author(s):  
Paulina Kubisz ◽  
Graham Dalton ◽  
Edward Majewski ◽  
Kinga Pogodzińska
Keyword(s):  
Global Economy ◽  
Genetically Modified Organisms ◽  
Human Life ◽  
Genetically Modified ◽  
Scientific Evidence ◽  
Negative Attitude ◽  
General Observation ◽  
Adequate Knowledge ◽  
Gm Food ◽  
Polish Society

The importance of biotechnology for the global economy is growing, including developments in the field of genetically modified organisms (GMO), which have revolutionized the cultivation of several major food crops. Despite the many benefits from introducing genetic modifications to crops, the Polish society shows a strong distrust towards GMO-based food. The negative attitude of the society towards genetically modified (GM) food could be considered irrational. It is not supported by adequate knowledge and is based on fears, despite the fact that there is no scientific evidence of threats of GM products towards the environment, health, or human life. Details of these perceptions were revealed within Polish society from surveys of three groups of respondents: consumers, students, farmers. Data from the surveys have been compared with the answers to the same questions by five biotechnology experts from Polish academic institutions. A general observation from the analysis of the survey results and past studies quoted in the literature review is that the level of understanding and acceptance of GMO technologies is still low in Polish society, and, to a large extent, is based on stereotypes rather than on scientific knowledge. They show broad support for the general benefits of GMOs, which does not vary between the three groups of respondents surveyed, but noticeably differs with the experts’ views. GMOs have allies, but also opponents who have their own beliefs shaped largely by unreliable information disseminated through the Internet and social media. Providing more reliable targeted information on GMOs based on scientific evidence can have an important role in changing polarized attitudes towards GM food.

scholarly journals MicroRNA Transformed Genetically Modified Crops and their Effect on Human Health.

2021 ◽  
Author(s):  
Sameer Quazi
Keyword(s):  
Human Health ◽  
Genetically Modified Organisms ◽  
Human Life ◽  
Genetically Modified ◽  
Genetically Modified Crops ◽  
Micro Rna ◽  
Gm Crops ◽  
Gm Plants ◽  
Micro Rnas ◽  
Wide Range

Abstract GM crops or Genetically Modified crops are attracted a wide range of media attention in recent years and continues to do so. Media given awareness about the genetically modified crops to public. They reported the uses and drawbacks of the GM crops. The technique offers with regards to the range of advantages of the use of genetically modified crops. In the Pioneer stage of the production of GM crops, two different sectors of concern have been evolved, which includes impact on the agriculture and dangerous to human life. Safety of the eatables have a vital role in the world. The issue can be reduced by enhancing the productivity and quality of the crop. Genetic manipulation technology depends on the MiRNA, it is one of the main problem-solving methods, which influence the environmental product formation through improving major rules used for miRNAs modification and its objectives in GM plants, it contains constitutive, induction to stress, or specific tissue expression of micro RNAs or their aim, RNA gene silencing mechanism, micro-RNA-resistant target and gene expressions. Genetically Modified Organisms is one of the major focuses in biomedical research from 1980s. Since, Genetically Modified models with animal enable researchers for treatment of human genetic diseases. Genetically Modified microorganisms, crops, and animals are used for the production of drugs that are complex by which helps the generation to vaccines that are cheaper. However, this article+ more focused on the human health associated with the genetically modified foods and role of miRNAs in respected to GM food products.

Prime-editors (nickases), hRad51–Cas9 nickase fusions and dCas9 have the same problem as conventional CRISPR-Cas9 of plasmid/Cas9 integration after making a double stranded break

2019 ◽  
Author(s):  
Sandeep Chakraborty
Keyword(s):  
Cellular Response ◽  
Sequencing Data ◽  
Dna Breaks ◽  
Precise Integration ◽  
Guide Rna ◽  
Double Strand Dna Breaks ◽  
Human Therapy ◽  
P53 Activation ◽  
Programmable Nucleases

‘Prime-editing’ proposes to replace traditional programmable nucleases (CRISPR-Cas9) using a catalytically impaired Cas9 (dCas9) connected to a engineered reverse transcriptase, and a guide RNA encoding both the target site and the desired change. With just a ‘nick’ on one strand, it is hypothe- sized, the negative, uncontrollable effects arising from double-strand DNA breaks (DSBs) - translocations, complex proteins, integrations and p53 activation - will be eliminated. However, sequencing data pro- vided (Accid:PRJNA565979) reveal plasmid integration, indicating that DSBs occur. Also, looking at only 16 off-targets is inadequate to assert that Prime-editing is more precise. Integration of plasmid occurs in all three versions (PE1/2/3). Interestingly, dCas9 which is known to be toxic in E. coli and yeast, is shown to have residual endonuclease activity. This also affects studies that use dCas9, like base- editors and de/methylations systems. Previous work using hRad51–Cas9 nickases also show significant integration in on-targets, as well as off-target integration [1]. Thus, we show that cellular response to nicking involves DSBs, and subsequent plasmid/Cas9 integration. This is an unacceptable outcome for any in vivo application in human therapy.

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