scholarly journals Transcriptome Analysis of Subcutaneous Adipose Tissue from Severely Obese Patients Highlights Deregulation Profiles in Coding and Non-Coding Oncogenes

2021 ◽  
Vol 22 (4) ◽  
pp. 1989
Author(s):  
Federica Rey ◽  
Letizia Messa ◽  
Cecilia Pandini ◽  
Rossella Launi ◽  
Bianca Barzaghini ◽  
...  
Keyword(s):  
Gender Differences ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Molecular Targets ◽  
Normal Weight ◽  
Obese Patients ◽  
Obese Women ◽  
Severely Obese ◽  
Cancer Types ◽  
Subcutaneous Adipose

Obesity is a major risk factor for a large number of secondary diseases, including cancer. Specific insights into the role of gender differences and secondary comorbidities, such as type 2 diabetes (T2D) and cancer risk, are yet to be fully identified. The aim of this study is thus to find a correlation between the transcriptional deregulation present in the subcutaneous adipose tissue of obese patients and the oncogenic signature present in multiple cancers, in the presence of T2D, and considering gender differences. The subcutaneous adipose tissue (SAT) of five healthy, normal-weight women, five obese women, five obese women with T2D and five obese men were subjected to RNA-sequencing, leading to the identification of deregulated coding and non-coding RNAs, classified for their oncogenic score. A panel of DE RNAs was validated via Real-Time PCR and oncogene expression levels correlated the oncogenes with anthropometrical parameters, highlighting significant trends. For each analyzed condition, we identified the deregulated pathways associated with cancer, the prediction of possible prognosis for different cancer types and the lncRNAs involved in oncogenic networks and tissues. Our results provided a comprehensive characterization of oncogenesis correlation in SAT, providing specific insights into the possible molecular targets implicated in this process. Indeed, the identification of deregulated oncogenes also in SAT highlights hypothetical targets implicated in the increased oncogenic risk in highly obese subjects. These results could shed light on new molecular targets to be specifically modulated in obesity and highlight which cancers should receive the most attention in terms of better prevention in obesity-affected patients.

In vitro lipid synthesis in human adipose tissue from three abdominal sites

1993 ◽  
Vol 265 (3) ◽  
pp. E374-E379 ◽  
Author(s):  
N. K. Edens ◽  
S. K. Fried ◽  
J. G. Kral ◽  
J. Hirsch ◽  
R. L. Leibel
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Lipid Synthesis ◽  
Human Adipose Tissue ◽  
Obese Women ◽  
Cellular Mechanisms ◽  
Severely Obese ◽  
Fatty Acid Release ◽  
Subcutaneous Adipose

The association between abdominal deposition of adipose tissue and morbidities accompanying obesity may be related to high rates of free fatty acid release from enlarged intra-abdominal stores. To investigate cellular mechanisms that might contribute to enlargement of intra-abdominal adipocytes, lipolysis, triacylglycerol (TG), and diacylglycerol (DG) synthesis from [14C]glucose was measured in abdominal subcutaneous, omental, and mesenteric adipose tissue from severely obese women and men. Subcutaneous adipose tissue from women showed the highest rates of TG synthesis compared with the intra-abdominal site, or any site in men. isoproterenol stimulated TG synthesis more in intra-abdominal than subcutaneous adipose tissue. In the basal state, intra-abdominal adipose tissue from both men and women showed rates of [14C]DG accumulation approximately 50% total [14C]acylglyceride accumulation, whereas, in subcutaneous adipose tissue, [14C]DG accumulation was approximately 25% of total. Basal lipolysis was lower in intra-abdominal than subcutaneous adipocytes. Stimulation of lipolysis reduced [14C]DG accumulation more in intra-abdominal than subcutaneous adipose tissue. Low rates of acylglyceride synthesis in intra-abdominal adipocytes may prevent accumulation of large intra-abdominal fat stores in women.

DISTRIBUTION OF GHRELIN RECEPTOR (GHSR-1A) ON ADIPOCYTES DERIVED FROM SUBCUTANEOUS ADIPOSE TISSUE IN SEVERELY OBESE PATIENTS

2018 ◽  
Vol 25 (3) ◽  
pp. 215-216
Author(s):  
Veronica Mocanu ◽  
Ioana Hristov ◽  
Adrian Tiron ◽  
Teodor Oboroceanu ◽  
Crina Tiron ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Obese Patients ◽  
Ghrelin Receptor ◽  
Severely Obese ◽  
Subcutaneous Adipose

Cefamandole Distribution in Serum, Adipose Tissue, and Wound Drainage in Morbidly Obese Patients

1986 ◽  
Vol 20 (11) ◽  
pp. 869-873 ◽  
Author(s):  
Henry J. Mann ◽  
Henry Buchwald
Keyword(s):  
Adipose Tissue ◽  
Rate Constant ◽  
Subcutaneous Adipose Tissue ◽  
Elimination Rate ◽  
Elimination Rate Constant ◽  
Morbidly Obese ◽  
Obese Patients ◽  
Tissue Concentrations ◽  
Wound Drainage ◽  
Subcutaneous Adipose

Distribution and elimination of cefamandole 2 g iv were studied in 11 morbidly obese patients during a gastric bypass operation and again on the first postoperative day. Serum, subcutaneous adipose tissue, wound drainage, and urine were analyzed by high performance liquid chromatography for cefamandole and pharmacokinetic parameters from the intraoperative period were compared to those obtained postoperatively. Total body clearance was significantly greater (p < 0.001) postoperatively (297 ml/min) than intraoperatively (254 ml/min). Volume changes were unpredictable but the elimination rate constant tended to increase postoperatively. Renal clearance and percentage of urinary recovery were significantly increased (p < 0.01) postoperatively. The patients had a mean (± SD) volume of the central compartment of 10.3 (± 2.3) L, volume at steady state of 18.3 (± 3.9) L, and elimination rate constant of 1.67 (± 0.63) h−1. Tissue concentrations of cefamandole were highest during the first hour after drug administration and were < 1 μg/g after 3.5 hours. Mean wound drainage concentrations ranged between 10 and 12 μg/ml during a dosing interval and dropped to 7 μg/ml 12 hours after the last dose. Intraoperative dosing of cefamandole is required to maintain subcutaneous adipose tissue concentrations > 1 μg/g during procedures longer than three hours in morbidly obese patients. A postoperative dose of cefamandole 2 g iv q6h will provide sustained and therapeutic concentrations in the wound drainage of morbidly obese patients.

scholarly journals Insulin resistance is associated with a modest increase in inflammation in subcutaneous adipose tissue of moderately obese women

Diabetologia ◽  
2008 ◽  
Vol 51 (12) ◽  
pp. 2303-2308 ◽  
Author(s):  
T. McLaughlin ◽  
A. Deng ◽  
O. Gonzales ◽  
M. Aillaud ◽  
G. Yee ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Obese Women ◽  
Modest Increase ◽  
Subcutaneous Adipose

scholarly journals Elevated Levels of Interleukin 6 Are Reduced in Serum and Subcutaneous Adipose Tissue of Obese Women after Weight Loss*

2000 ◽  
Vol 85 (9) ◽  
pp. 3338-3342 ◽  
Author(s):  
Jean-Philippe Bastard ◽  
Claude Jardel ◽  
Eric Bruckert ◽  
Patricia Blondy ◽  
Jacqueline Capeau ◽  
...  
Keyword(s):  
Weight Loss ◽  
Adipose Tissue ◽  
Interleukin 6 ◽  
Subcutaneous Adipose Tissue ◽  
Obese Women ◽  
Subcutaneous Adipose

scholarly journals Downregulation of de Novo Fatty Acid Synthesis in Subcutaneous Adipose Tissue of Moderately Obese Women

2015 ◽  
Vol 16 (12) ◽  
pp. 29911-29922 ◽  
Author(s):  
Esther Guiu-Jurado ◽  
Teresa Auguet ◽  
Alba Berlanga ◽  
Gemma Aragonès ◽  
Carmen Aguilar ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Subcutaneous Adipose Tissue ◽  
De Novo ◽  
Acid Synthesis ◽  
Obese Women ◽  
Subcutaneous Adipose
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