DISTRIBUTION OF GHRELIN RECEPTOR (GHSR-1A) ON ADIPOCYTES DERIVED FROM SUBCUTANEOUS ADIPOSE TISSUE IN SEVERELY OBESE PATIENTS

2018 ◽  
Vol 25 (3) ◽  
pp. 215-216
Author(s):  
Veronica Mocanu ◽  
Ioana Hristov ◽  
Adrian Tiron ◽  
Teodor Oboroceanu ◽  
Crina Tiron ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Obese Patients ◽  
Ghrelin Receptor ◽  
Severely Obese ◽  
Subcutaneous Adipose

scholarly journals Transcriptome Analysis of Subcutaneous Adipose Tissue from Severely Obese Patients Highlights Deregulation Profiles in Coding and Non-Coding Oncogenes

2021 ◽  
Vol 22 (4) ◽  
pp. 1989
Author(s):  
Federica Rey ◽  
Letizia Messa ◽  
Cecilia Pandini ◽  
Rossella Launi ◽  
Bianca Barzaghini ◽  
...  
Keyword(s):  
Gender Differences ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Molecular Targets ◽  
Normal Weight ◽  
Obese Patients ◽  
Obese Women ◽  
Severely Obese ◽  
Cancer Types ◽  
Subcutaneous Adipose

Obesity is a major risk factor for a large number of secondary diseases, including cancer. Specific insights into the role of gender differences and secondary comorbidities, such as type 2 diabetes (T2D) and cancer risk, are yet to be fully identified. The aim of this study is thus to find a correlation between the transcriptional deregulation present in the subcutaneous adipose tissue of obese patients and the oncogenic signature present in multiple cancers, in the presence of T2D, and considering gender differences. The subcutaneous adipose tissue (SAT) of five healthy, normal-weight women, five obese women, five obese women with T2D and five obese men were subjected to RNA-sequencing, leading to the identification of deregulated coding and non-coding RNAs, classified for their oncogenic score. A panel of DE RNAs was validated via Real-Time PCR and oncogene expression levels correlated the oncogenes with anthropometrical parameters, highlighting significant trends. For each analyzed condition, we identified the deregulated pathways associated with cancer, the prediction of possible prognosis for different cancer types and the lncRNAs involved in oncogenic networks and tissues. Our results provided a comprehensive characterization of oncogenesis correlation in SAT, providing specific insights into the possible molecular targets implicated in this process. Indeed, the identification of deregulated oncogenes also in SAT highlights hypothetical targets implicated in the increased oncogenic risk in highly obese subjects. These results could shed light on new molecular targets to be specifically modulated in obesity and highlight which cancers should receive the most attention in terms of better prevention in obesity-affected patients.

Cefamandole Distribution in Serum, Adipose Tissue, and Wound Drainage in Morbidly Obese Patients

1986 ◽  
Vol 20 (11) ◽  
pp. 869-873 ◽  
Author(s):  
Henry J. Mann ◽  
Henry Buchwald
Keyword(s):  
Adipose Tissue ◽  
Rate Constant ◽  
Subcutaneous Adipose Tissue ◽  
Elimination Rate ◽  
Elimination Rate Constant ◽  
Morbidly Obese ◽  
Obese Patients ◽  
Tissue Concentrations ◽  
Wound Drainage ◽  
Subcutaneous Adipose

Distribution and elimination of cefamandole 2 g iv were studied in 11 morbidly obese patients during a gastric bypass operation and again on the first postoperative day. Serum, subcutaneous adipose tissue, wound drainage, and urine were analyzed by high performance liquid chromatography for cefamandole and pharmacokinetic parameters from the intraoperative period were compared to those obtained postoperatively. Total body clearance was significantly greater (p < 0.001) postoperatively (297 ml/min) than intraoperatively (254 ml/min). Volume changes were unpredictable but the elimination rate constant tended to increase postoperatively. Renal clearance and percentage of urinary recovery were significantly increased (p < 0.01) postoperatively. The patients had a mean (± SD) volume of the central compartment of 10.3 (± 2.3) L, volume at steady state of 18.3 (± 3.9) L, and elimination rate constant of 1.67 (± 0.63) h−1. Tissue concentrations of cefamandole were highest during the first hour after drug administration and were < 1 μg/g after 3.5 hours. Mean wound drainage concentrations ranged between 10 and 12 μg/ml during a dosing interval and dropped to 7 μg/ml 12 hours after the last dose. Intraoperative dosing of cefamandole is required to maintain subcutaneous adipose tissue concentrations > 1 μg/g during procedures longer than three hours in morbidly obese patients. A postoperative dose of cefamandole 2 g iv q6h will provide sustained and therapeutic concentrations in the wound drainage of morbidly obese patients.

Staged ultrasound-guided liposuction for hidden arteriovenous fistulas in obese patients

VASA ◽  
2018 ◽  
Vol 47 (5) ◽  
pp. 403-407
Author(s):  
Gabor Cs.Nagy ◽  
Reiner Verwiebe ◽  
Matthias Wunsch
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Relevant Literature ◽  
Skin Surface ◽  
Obese Patients ◽  
Ultrasound Guided ◽  
Arteriovenous Fistulas ◽  
The Mean ◽  
Stage Renal Disease ◽  
Subcutaneous Adipose

Abstract. Background: In obese patients with end stage renal disease, puncturing matured arteriovenous fistulas (AVF) that run deep under the skin surface may prove difficult. To achieve reliable puncturability, there are several surgical solutions. Superficialization with mobilization is common. With some newer options (lipectomy and liposuction) subcutaneous adipose tissue is surgically reduced. There are only a few authors who have published their experience with liposuction and we want to add our own results. Patients and methods: We report our experience with ultrasound-guided liposuction (USGL). We introduce liposuction cannulas via small incisions to reduce the subcutaneous adipose tissue overlying the planned cannulation zones under ultrasound control using tumescent anaesthesia till the prospective needle access segments become easily palpable. So far, we have used this technique for cephalic forearm and upper arm fistulas only. Furthermore, we review the relevant literature. Results: From February 2014 through November 2016, six patients were treated using USGL. Their body mass indices ranged from 30.8 to 53.8 kg/m2 (mean 37.6). The mean depths of the AVFs beneath the skin surface were 13.3 (8–20) mm before and 5.1 (3.5–6) mm after surgery. The mean time of the procedure was 15 minutes. There were no postoperative complications. In five patients, the AVFs could reliably be punctured after three weeks. One patient is not yet on dialysis. During the follow-up period of 24 (11–43) months, all six AVFs remained primarily patent. In the literature, we found nine reports on altogether 81 patients undergoing USGL. Almost all noteworthy complications occurred only after ultrasound-powered liquefaction of adipose tissue, which was only used by a single investigator. Conclusions: USGL is a method that can be learned easily, is minimally invasive, seems to be safe, and requires only short operation times.

2313-PUB: Alterations in Basal State of Insulin and mTOR-Dependent Signalings Closely Related to Impaired Incretin Profile and Type 2 Diabetes in Subcutaneous Adipose Tissue of Obese Patients

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 2313-PUB
Author(s):  
IURII STAFEEV ◽  
IGOR SKLYANIK ◽  
SVETLANA MICHURINA ◽  
EKATERINA SHESTAKOVA ◽  
ANATOLIY YURASOV ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Obese Patients ◽  
Subcutaneous Adipose

scholarly journals Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Carmela Nardelli ◽  
Laura Iaffaldano ◽  
Vincenzo Pilone ◽  
Giuseppe Labruna ◽  
Maddalena Ferrigno ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Gastric Banding ◽  
Laparoscopic Adjustable Gastric Banding ◽  
Subcutaneous Adipose Tissue ◽  
Adjustable Gastric Banding ◽  
Epigenetic Mechanism ◽  
Mirna Profile ◽  
Obese Patients ◽  
Subcutaneous Adipose

Background. Laparoscopic adjustable gastric banding (LAGB) results in significant lasting weight loss and improved metabolism in obese patients. To evaluate whether epigenetic factors could concur to these benefits, we investigated the subcutaneous adipose tissue (SAT) microRNA (miRNA) profile before (T0) and three years (T1) after LAGB in three morbidly obese women. Case Reports. SAT miRNA profiling, evaluated by TaqMan Array, showed four downexpressed (miR-519d, miR-299-5p, miR-212, and miR-671-3p) and two upexpressed (miR-370 and miR-487a) miRNAs at T1 versus T0. Bioinformatics predicted that these miRNAs regulate genes belonging to pathways associated with the cytoskeleton, inflammation, and metabolism. Western blot analysis showed that PPAR-alpha, which is the target gene of miR-519d, increased after LAGB, thereby suggesting an improvement in SAT lipid metabolism. Accordingly, the number and diameter of adipocytes were significantly higher and lower, respectively, at T1 versus T0. Bioinformatics predicted that the decreased levels of miR-212, miR-299-5p, and miR-671-3p at T1 concur in reducing SAT inflammation. Conclusion. We show that the miRNA profile changes after LAGB. This finding, although obtained in only three cases, suggests that this epigenetic mechanism, by regulating the expression of genes involved in inflammation and lipid metabolism, could concur to improve SAT functionality in postoperative obese patients.

11β-Hydroxysteroid Dehydrogenase Type 1 mRNA is Increased in Both Visceral and Subcutaneous Adipose Tissue of Obese Patients*

Obesity ◽  
2006 ◽  
Vol 14 (5) ◽  
pp. 794-798 ◽  
Author(s):  
Raoul Desbriere ◽  
Vincent Vuaroqueaux ◽  
Vincent Achard ◽  
Sandrine Boullu-Ciocca ◽  
Martin Labuhn ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Hydroxysteroid Dehydrogenase ◽  
Obese Patients ◽  
Subcutaneous Adipose

STEAP4 and HIF-1α gene expressions in visceral and subcutaneous adipose tissue of the morbidly obese patients

2016 ◽  
Vol 73 ◽  
pp. 53-59 ◽  
Author(s):  
Fusun Ozmen ◽  
M. Mahir Ozmen ◽  
Sibel Gelecek ◽  
İsmail Bilgic ◽  
Munevver Moran ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Morbidly Obese ◽  
Obese Patients ◽  
Gene Expressions ◽  
Subcutaneous Adipose
Sign in / Sign up

Export Citation Format

Share Document