scholarly journals Evaluation of the Multimycotoxin-Degrading Efficiency of Rhodococcus erythropolis NI1 Strain with the Three-Step Zebrafish Microinjection Method

2021 ◽  
Vol 22 (2) ◽  
pp. 724
Author(s):  
Edina Garai ◽  
Anita Risa ◽  
Emese Varga ◽  
Mátyás Cserháti ◽  
Balázs Kriszt ◽  
...  
Keyword(s):  
Degradation Products ◽  
Synergistic Effects ◽  
Rhodococcus Erythropolis ◽  
Toxic Effects ◽  
Zebrafish Embryos ◽  
Combined Exposure ◽  
Step Method ◽  
Cell Stage ◽  
Antagonistic Effects ◽  
Very High

The multimycotoxin-degrading efficiency of the Rhodococcus erythropolis NI1 strain was investigated with a previously developed three-step method. NI1 bacterial metabolites, single and combined mycotoxins and their NI1 degradation products, were injected into one cell stage zebrafish embryos in the same doses. Toxic and interaction effects were supplemented with UHPLC-MS/MS measurement of toxin concentrations. Results showed that the NI1 strain was able to degrade mycotoxins and their mixtures in different proportions, where a higher ratio of mycotoxins were reduced in combination than single ones. The NI1 strain reduced the toxic effects of mycotoxins and mixtures, except for the AFB1+T-2 mixture. Degradation products of the AFB1+T-2 mixture by the NI1 strain were more toxic than the initial AFB1+T-2 mixture, while the analytical results showed very high degradation, which means that the NI1 strain degraded this mixture to toxic degradation products. The NI1 strain was able to detoxify the AFB1, ZEN, T-2 toxins and mixtures (except for AFB1+T-2 mixture) during the degradation experiments, which means that the NI1 strain degraded these to non-toxic degradation products. The results demonstrate that single exposures of mycotoxins were very toxic. The combined exposure of mycotoxins had synergistic effects, except for ZEN+T-2 and AFB1+ZEN +T-2, whose mixtures had very strong antagonistic effects.

scholarly journals Qualifying the T-2 Toxin-Degrading Properties of Seven Microbes with Zebrafish Embryo Microinjection Method

Toxins ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 460
Author(s):  
Edina Garai ◽  
Anita Risa ◽  
Emese Varga ◽  
Mátyás Cserháti ◽  
Balázs Kriszt ◽  
...  
Keyword(s):  
Sublethal Effects ◽  
Degradation Products ◽  
Rhodococcus Erythropolis ◽  
Bacterial Strains ◽  
Cell Stage ◽  
Bacterial Metabolites ◽  
Mycotoxin Degradation

T-2 mycotoxin degradation and detoxification efficiency of seven bacterial strains were investigated with zebrafish microinjection method in three steps ((1) determination of mycotoxin toxicity baseline, (2) examination of bacterial metabolites toxicity, (3) identification of degradation products toxicity). Toxicity of T-2 was used as a baseline of toxic effects, bacterial metabolites of strains as control of bacterial toxicity and degradation products of toxin as control of biodegradation were injected into one-cell stage embryos in the same experiment. The results of in vivo tests were checked and supplemented with UHPLC-MS/MS measurement of T-2 concentration of samples. Results showed that the Rhodococcus erythropolis NI1 strain was the only one of the seven tested (R. gordoniae AK38, R. ruber N361, R. coprophilus N774, R. rhodochrous NI2, R. globerulus N58, Gordonia paraffinivorans NZS14), which was appropriated to criteria all aspects (bacterial and degradation metabolites of strains caused lower toxicity effects than T-2, and strains were able to degrade T-2 mycotoxin). Bacterial and degradation metabolites of the NI1 strain caused slight lethal and sublethal effects on zebrafish embryos at 72- and 120-h postinjection. Results demonstrated that the three-step zebrafish microinjection method is well-suited to the determination and classification of different bacterial strains by their mycotoxin degradation and detoxification efficiency.

Synthesis and Electrochemical Properties of NiFe-Se/rGO Nanocomposites

2020 ◽  
Vol 13 ◽  
Author(s):  
Rouwei Yan ◽  
Biao Xu ◽  
K. P. Annamalai ◽  
Tianlu Chen ◽  
Zhiming Nie ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Graphene Oxide ◽  
Energy Storage ◽  
Synergistic Effects ◽  
Nitrogen Adsorption ◽  
Storage Device ◽  
Energy Storage And Conversion ◽  
Step Method ◽  
Practical Applications ◽  
Device Applications

Background : Renewable energies are in great demand because of the shortage of traditional fossil energy and the associated environmental problems. Ni and Se-based materials are recently studied for energy storage and conversion owing to their reasonable conductivities and enriched redox activities as well as abundance. However, their electrochemical performance is still unsatisfactory for practical applications. Objective: To enhance the capacitance storage of Ni-Se materials via modification of their physiochemical properties with Fe. Methods: A two-step method was carried out to prepare FeNi-Se loaded reduced graphene oxide (FeNi-Se/rGO). In the first step, metal salts and graphene oxide (GO) were mixed under basic condition and autoclaved to obtain hydroxide intermediates. As a second step, selenization process was carried out to acquire FeNi-Se/rGO composites. Results: X-ray diffraction measurements (XRD), nitrogen adsorption at 77K, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were carried out to study the structures, porosities and the morphologies of the composites. Electrochemical measurements revealed that FeNi-Se/rGO notably enhanced capacitance than the NiSe/G composite. This enhanced performance was mainly attributed to the positive synergistic effects of Fe and Ni in the composites, which not only had influence on the conductivity of the composite but also enhanced redox reactions at different current densities. Conclusion: NiFe-Se/rGO nanocomposites were synthesized in a facile way. The samples were characterized physicochemically and electrochemically. NiFeSe/rGO giving much higher capacitance storage than the NiSe/rGO explained that the nanocomposites could be an electrode material for energy storage device applications.

Emerging contaminant occurrence and toxic effects on zebrafish embryos to assess the adverse effects caused by mixtures of substances in the environment

2021 ◽  
Author(s):  
Gisela de Assis Martini ◽  
Cassiana Carolina Montagner ◽  
William Viveiros ◽  
Gilson Alves Quinaglia ◽  
Daniela Dayrell França ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Toxic Effects ◽  
Zebrafish Embryos ◽  
Emerging Contaminant

Interactions of Caffeine and Restraint Stress During Pregnancy in Mice

2002 ◽  
Vol 227 (9) ◽  
pp. 779-785 ◽  
Author(s):  
M. Luisa Albina ◽  
M. Teresa Colomina ◽  
Domenec J. Sanchez ◽  
Margarita Torrente ◽  
Jose L. Domingo
Keyword(s):  
Restraint Stress ◽  
Developmental Toxicity ◽  
Experimental Period ◽  
Toxic Effects ◽  
Cesarean Sections ◽  
Combined Exposure ◽  
Control Groups ◽  
Fetal Toxicity ◽  
Skeletal Malformations ◽  
Developmental Effects

The maternal and developmental toxicity of combined exposure to restraint stress and caffeine was assessed in mice. On gestational Days 0–18, three groups of plug-positive females (n = 13–15) were given by gavage caffeine at 30, 60, and 120 mg/kg/day. Three additional groups received the same caffeine doses and were restrained for 2 hr/day. Control groups included restrained and unrestrained plug-positive mice not exposed to caffeine. All animals in the group concurrently exposed to 120 mg/kg/day of caffeine and restraint died during the experimental period. In the remaining groups, cesarean sections were performed on Day 18 of gestation, and the fetuses were weighed and examined for external, internal, and skeletal malformations and variations. Although maternal and embryo/fetal toxicity were observed at all caffeine doses, the adverse maternal and developmental effects were significantly enhanced in the groups concurrently exposed to caffeine and restraint. It was especially remarkable at 60 and 120 mg/kg/day. The results of this study suggest that maternal and developmental toxic effects might occur if high amounts of caffeine were consumed by women under a notable stress during pregnancy.

Particle-specific toxic effects of differently shaped zinc oxide nanoparticles to zebrafish embryos (Danio rerio )

2014 ◽  
Vol 33 (12) ◽  
pp. 2859-2868 ◽  
Author(s):  
Jing Hua ◽  
Martina G. Vijver ◽  
Michael K. Richardson ◽  
Farooq Ahmad ◽  
Willie J.G.M. Peijnenburg
Keyword(s):  
Zinc Oxide ◽  
Danio Rerio ◽  
Zinc Oxide Nanoparticles ◽  
Toxic Effects ◽  
Oxide Nanoparticles ◽  
Zebrafish Embryos

Exposure to the azo dye Direct blue 15 produces toxic effects on microalgae, cladocerans, and zebrafish embryos

Ecotoxicology ◽  
2019 ◽  
Vol 28 (8) ◽  
pp. 890-902 ◽  
Author(s):  
Miriam Hernández-Zamora ◽  
Fernando Martínez-Jerónimo
Keyword(s):  
Azo Dye ◽  
Toxic Effects ◽  
Zebrafish Embryos ◽  
Direct Blue ◽  
Direct Blue 15

scholarly journals Comparison of the in vitro and in vivo toxic effects of thymoquinone using oral cancer HSC-3 and HSC-4 cell lines, oral fibroblasts, HACAT cell line, and Zebrafish embryos

2019 ◽  
Vol 16 ◽  
pp. 2108-2114
Author(s):  
Wastuti Hidayati Suriyah ◽  
Abdul Razak Kasmuri ◽  
Fiona How Ni Foong ◽  
Dhona Afriza ◽  
Solachuddin Jauhari Arief Ichwan
Keyword(s):  
Oral Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Hacat Cell ◽  
Toxic Effects ◽  
Zebrafish Embryos ◽  
Hacat Cell Line

Imaging intercellular calcium waves during late epiboly in intact zebrafish embryos

Zygote ◽  
2003 ◽  
Vol 11 (2) ◽  
pp. 175-182 ◽  
Author(s):  
Sarah E. Webb ◽  
Andrew L. Miller
Keyword(s):  
Zebrafish Embryos ◽  
Calcium Waves ◽  
Embryonic Patterning ◽  
Cell Stage ◽  
Appearance Time ◽  
Scanning Confocal Microscopy ◽  
High Resolution Images ◽  
Time Propagation ◽  
Pass Through ◽  
The Waves

Through the injection of f-aequorin and the use of a photon imaging microscope, we have previously reported that a rhythmic series of intercellular Ca2+ waves circumnavigate zebrafish embryos over a 10 h period during gastrulation and axial segmentation. These waves first appear at about 65% epiboly and continue to arise every 5-10 min up to at least the 16-somite stage. In response to our publication, it was suggested that the waves may be an artefact caused by dechorionation of the embryos and would not be observed during the development of intact embryos (i.e. those with chorions). Here we demonstrate (again initially by aequorin imaging) that the rhythmic intercellular Ca2+ waves that traverse the blastoderm margin can also be observed in embryos that have an intact chorion. In addition, the appearance time, propagation pathway, velocity, duration and Ca2+ rise of the waves, as well as the interwave interval and the timing of wave onset, are approximately the same in both dechorionated embryos and those with an intact chorion. Furthermore, by loading intact embryos with Ca2+-green dextran at the single-cell stage and then using scanning confocal microscopy to obtain high-resolution images, we confirm the presence of circumferential Ca2+ waves and show that they pass through a population of deep cells located at the blastoderm margin. The confirmation of these pan-embryonic Ca2+ waves in zebrafish further corroborates our earlier suggestion that such waves might play a fundamental role in normal embryonic patterning during the gastrula period.

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