scholarly journals The Interplay between Fe3O4 Superparamagnetic Nanoparticles, Sodium Butyrate, and Folic Acid for Intracellular Transport

2020 ◽  
Vol 21 (22) ◽  
pp. 8473
Author(s):  
Maria Teresa Cambria ◽  
Giusy Villaggio ◽  
Samuele Laudani ◽  
Luca Pulvirenti ◽  
Concetta Federico ◽  
...  
Keyword(s):  
Folic Acid ◽  
Sodium Butyrate ◽  
Intracellular Transport ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Superparamagnetic Nanoparticles ◽  
High Dose ◽  
Dependent Manner ◽  
The Difference ◽  
Surface Modified

Combined treatments which use nanoparticles and drugs could be a synergistic strategy for the treatment of a variety of cancers to overcome drug resistance, low efficacy, and high-dose-induced systemic toxicity. In this study, the effects on human colon adenocarcinoma cells of surface modified Fe3O4 magnetic nanoparticles (MNPs) in combination with sodium butyrate (NaBu), added as a free formulation, were examined demonstrating that the co-delivery produced a cytotoxic effect on malignant cells. Two different MNP coatings were investigated: a simple polyethylene glycol (PEG) layer and a mixed folic acid (FA) and PEG layer. Our results demonstrated that MNPs with FA (FA-PEG@MNPs) have a better cellular uptake than the ones without FA (PEG@MNPs), probably due to the presence of folate that acts as an activator of folate receptors (FRs) expression. However, in the presence of NaBu, the difference between the two types of MNPs was reduced. These similar behaviors for both MNPs likely occurred because of the differentiation induced by butyrate that increases the uptake of ferromagnetic nanoparticles. Moreover, we observed a strong decrease of cell viability in a NaBu dose-dependent manner. Taking into account these results, the cooperation of multifunctional MNPs with NaBu, taking into consideration the particular cancer-cell properties, can be a valuable tool for future cancer treatment.

Stable differentiation of a human colon adenocarcinoma cell line by sodium butyrate is associated with multidrug resistance

1994 ◽  
Vol 160 (2) ◽  
pp. 213-226 ◽  
Author(s):  
Samuel B. Ho ◽  
Pei-Sha Yan ◽  
Rajvir Dahiya ◽  
Brent A. Neuschwander-Tetri ◽  
Carol Basbaum ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Cell Line ◽  
Sodium Butyrate ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Human Colon Adenocarcinoma

scholarly journals Safed Musli (Chlorophytum borivilianum L.) Callus-Mediated Biosynthesis of Silver Nanoparticles and Evaluation of their Antimicrobial Activity and Cytotoxicity against Human Colon Cancer Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Fengchang Huang ◽  
Yaxin Long ◽  
Qingqing Liang ◽  
Boregowda Purushotham ◽  
Mallappa Kumara Swamy ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Dependent Manner ◽  
Face Centered Cubic ◽  
Human Colon Cancer ◽  
Chlorophytum Borivilianum ◽  
The Face ◽  
Average Size ◽  
Safed Musli

With the advancement of nanobiotechnology, eco-friendly approaches of plant-mediated silver nanomaterial (AgNP) biosynthesis have become more attractive for biomedical applications. The present study is a report of biosynthesizing AgNPs using Chlorophytum borivilianum L. (Safed musli) callus extract as a novel source of reducing agent. AgNO3 solution challenged with the methanolic callus extract displayed a change in color from yellow to brown owing to the bioreduction reaction. Further, AgNPs were characterized by using UV–visible spectrophotometry, X-ray Diffraction (XRD), Atomic Force Microscopy (AFM), and Fourier Transform Infrared Spectroscopy (FTIR). UV–vis spectrum revealed the surface plasmon resonance property of AgNPs at around 450 nm. XRD pattern with typical peaks indicated the face-centered cubic nature of silver. AFM analysis confirmed the existence of spherical-shaped and well-dispersed AgNPs having an average size of 52.0 nm. Further, FTIR analysis confirmed the involvement of different phytoconstituents of the callus extract role in the process of bioreduction to form nanoparticles. The AgNPs were more efficient in inhibiting the tested pathogenic microbes, namely, Pseudomonas aeruginosa, Bacillus subtilis, Methicillin-resistant Escherichia coli, Staphylococcus aureus, and Candida albicans compared to callus extract. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay confirmed the cytotoxic property of AgNPs against human colon adenocarcinoma cell line (HT-29) in a dose-dependent manner. At higher concentrations of 500 μg/mL AgNPs, the cell viability was observed to be only 7% after 24 hours with IC50 value of 254 μg/mL. Therefore, these AgNPs clearly endorse the manifold potential to be used in various biomedical applications in the near future.

scholarly journals Phosphofructokinase 2 and glycolysis in HT29 human colon adenocarcinoma cell line. Regulation by insulin and phorbol esters

1990 ◽  
Vol 268 (2) ◽  
pp. 465-470 ◽  
Author(s):  
C Denis-Pouxviel ◽  
T Gauthier ◽  
D Daviaud ◽  
J C Murat
Keyword(s):  
Phorbol Esters ◽  
Lactate Production ◽  
Colon Adenocarcinoma ◽  
Glucose Consumption ◽  
Human Colon ◽  
Kinetic Properties ◽  
Dependent Manner ◽  
Glycerol Phosphate ◽  
Ht29 Cells ◽  
Long Term Treatment

Kinetic properties of phosphofructokinase 2 (PFK2) and regulation of glycolysis by phorbol 12-myristate 13-acetate (PMA) and insulin were investigated in highly glycolytic HT29 colon cancer cells. PFK2 was found to be inhibited by citrate and, to a lesser extent, by phosphoenolpyruvate and ADP, but to be insensitive to inhibition by sn-glycerol phosphate. From these kinetic data, PFK2 from HT29 cells appears different from the liver form, but resembles somewhat the heart isoenzyme. Fructose 2,6-bisphosphate (Fru-2,6-P2) levels, glucose consumption and lactate production are increased in a dose-dependent manner in HT29 cells treated with PMA or insulin. The increase in Fru-2,6-P2 can be related to an increase in the Vmax. of PFK2, persisting after the enzyme has been precipitated with poly(ethylene glycol), without change in the Km for fructose 6-phosphate. The most striking effects of PMA and insulin on Fru-2,6-P2 production are observed after long-term treatment (24 h) and are abolished by actinomycin, cycloheximide and puromycin, suggesting that protein synthesis is involved. Furthermore, the effects of insulin and PMA on glucose consumption, lactate production, Fru-2,6-P2 levels and PFK2 activity are additive, and the effect of insulin on Fru-2,6-P2 production is not altered by pre-treatment of the cells with the phorbol ester. This suggests that these effects are exerted by separate mechanisms.

Expression of IGF-II and IGF binding proteins in differentiating human intestinal Caco-2 cells

1995 ◽  
Vol 269 (5) ◽  
pp. E804-E813 ◽  
Author(s):  
Y. Zhang ◽  
D. A. Wick ◽  
B. Seetharam ◽  
N. M. Dahms
Keyword(s):  
Conditioned Medium ◽  
Binding Proteins ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Metabolic Effects ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Proliferating Cells ◽  
Igf Binding Proteins ◽  
Igf Binding

The mitogenic and metabolic effects of insulin-like growth factor-II (IGF-II) can be modulated by six distinct IGF binding proteins (IGFBPs). As a first step toward understanding the role of IGFs and their binding proteins in intestinal epithelial cell differentiation, the expression of IGF-II and IGFBPs was characterized in the human colon adenocarcinoma Caco-2 cell line. Northern blot analysis revealed two IGF-II transcripts of 5.4 and 4.5 kb, and ribonuclease protection assays indicated that IGF-II mRNA levels are regulated during Caco-2 differentiation. A specific radioimmunoassay detected IGF-II in serum-free conditioned medium, the level of which was three- to fivefold higher in proliferating cells than in differentiated cells. Immunoprecipitation and ligand blot analyses of conditioned medium demonstrated that IGFBP-2, IGFBP-3, IGFBP-4, and IGFBP-6 are synthesized by Caco-2 cells, with IGFBP-2 and IGFBP-4 being the major IGFBPs secreted, and that the levels of IGFBP-2 and IGFBP-6 decreased as differentiation proceeded. These results indicate that the expression of IGF-II, IGFBP-2, and IGFBP-6 is regulated in a differentiation-dependent manner in Caco-2 cells.

scholarly journals Glutathione conjugation of chlorambucil: measurement and modulation by plant polyphenols

1997 ◽  
Vol 325 (2) ◽  
pp. 417-422 ◽  
Author(s):  
Kai ZHANG ◽  
Kim Ping WONG
Keyword(s):  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Thymidine Uptake ◽  
Dependent Manner ◽  
Glutathione S Transferase ◽  
Plant Polyphenols ◽  
Simultaneous Exposure ◽  
Adenocarcinoma Cells ◽  
Combined Action ◽  
Dose Dependent

Chlorambucil (CMB), an anticancer drug, was cytotoxic at concentrations of 5–20 μM to human colon adenocarcinoma cells. It inhibited [14C]thymidine uptake in a dose-dependent manner. Both effects were potentiated by simultaneous exposure of the cells to 10 μM plant polyphenols. In an attempt to explain the possible mechanism of action of the polyphenols in relation to these observations, an HPLC-radiometric method was developed to measure the conjugation of CMB with glutathione in these cells and to monitor the export of monochloromonoglutathionyl CMB (MG-CMB), its main glutathione conjugate. At micromolar concentrations, five polyphenols, namely quercetin, butein, tannic acid, 2′-hydroxychalcone and morin, inhibited the efflux of CMB significantly; an inhibition of 40% was observed with 10 μM quercetin. The glutathione S-transferase (GST) activity of the cancer cells, measured with 1-chloro-2,4-dinitrobenzene, was also inhibited by the polyphenols. Their combined action on GST and on the efflux of MG-CMB conjugate could provide an enhanced positive modulation of sensitivity of the tumour cells to CMB.

scholarly journals In vitroandin vivoactivities of a bi-aryl oxazolidinone RBx 11760 against Gram positive bacteria

2016 ◽  
pp. AAC.00453-16 ◽  
Author(s):  
Tarani Kanta Barman ◽  
Manoj Kumar ◽  
Tarun Mathur ◽  
Tridib Chaira ◽  
G. Ramkumar ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
High Dose ◽  
Dependent Manner ◽  
Gram Positive ◽  
Concentration Dependent Manner ◽  
Gram Positive Bacteria ◽  
Initial Control ◽  
The Difference ◽  
Time Kill ◽  
Groin Abscess

RBx 11760, a bi-aryl oxazolidinone was investigated for antibacterial activity against Gram positive bacteria. The MIC90(mg/L) of RBx 11760 and linezolid againstStaphylococcus aureuswere: 2 and 4,Staphylococcus epidermidis: 0.5 and 2,Enterococcus: 1 and 4, respectively. Similarly againstStreptococcus pneumoniaeMIC90was: 0.5 and 2, respectively. In time-kill studies, RBx 11760, tedizolid and linezolid exhibited bacteriostatic effect exceptS. pneumoniae. RBx 11760 showed 2-log10kill at 4 X MIC while tedizolid and linezolid showed 2 log10and 1.4-log10kill at 16 X MIC, respectively against MRSA H-29. AgainstS. pneumoniae5051, RBx 11760 showed bactericidal activity with 4.6 log10kill at 4 X MIC compared to 2.42 log10and 1.95 log10kill of tedizolid and linezolid at 16 X MIC. RBx 11760 showed 3 h post antibiotic effects (PAE) at 4 mg/L against MRSA H-29 and linezolid showed same effect at 16mg/L. RBx 11760 inhibited the biofilm production against MRSE ATCC 35984 in concentration dependent manner. In foreign body model, linezolid and rifampicin resulted in no advantage over stasis, while same dose of RBx 11760 demonstrated a significant killing from initial control againstS. aureus(*p<0.05) and MRSE (**p<0.01). The difference in killing was statistically significant for the lower dose of RBx 11760 (*p<0.05) versus high dose of linezolid (nsp>0.05) in groin abscess model. In neutropenic mouse thigh infection, RBx 11760 showed stasis at 20 mg/kg whereas tedizolid showed same effect at 40 mg/kg. These data support the RBx 11760 as a promising investigational candidate.

scholarly journals Genoprotective Properties and Metabolites of β-Glucan-Rich Edible Mushrooms Following Their In Vitro Fermentation by Human Faecal Microbiota

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3554
Author(s):  
Athina Boulaka ◽  
Paraschos Christodoulou ◽  
Marigoula Vlassopoulou ◽  
Georgios Koutrotsios ◽  
Georgios Bekiaris ◽  
...  
Keyword(s):  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Edible Mushrooms ◽  
Dependent Manner ◽  
Fungal Cell ◽  
Tert Butyl Hydroperoxide ◽  
Adenocarcinoma Cells ◽  
Health Promoting ◽  
Dose Dependent

A variety of bioactive compounds, constituents of edible mushrooms, in particular β-glucans, i.e., a group of β-d-glucose polysaccharides abundant in the fungal cell walls, have been linked to immunomodulating, anticancer and prebiotic activities. The aim of the study was the investigation of the genoprotective effects of edible mushrooms produced by Pleurotus eryngii, Pleurotus ostreatus and Cyclocybe cylindracea (Basidiomycota). Mushrooms from selected strains of the species mentioned above were fermented in vitro using faecal inocula from healthy volunteers. The cytotoxic and anti-genotoxic properties of the fermentation supernatants (FSs) were investigated in Caco-2 human colon adenocarcinoma cells. The FSs were cytotoxic in a dose-dependent manner. Non-cytotoxic concentrations were used for the genotoxicity studies, which revealed that mushrooms’ FSs have the ability to protect Caco-2 cells against tert-butyl hydroperoxide (t-BOOH), a known genotoxic agent. Their global metabolic profiling was assessed by 1H-NMR spectroscopy. A total of 37 metabolites were identified with the use of two-dimensional (2D) homo- and hetero-nuclear NMR experiments. Multivariate data analysis monitored the metabolic variability of gut microbiota and probed to biomarkers potentially associated with the health-promoting effects of edible mushrooms.

scholarly journals Kinetic analysis of butyrate transport in human colon adenocarcinoma cells reveals two different carrier-mediated mechanisms

2007 ◽  
Vol 409 (1) ◽  
pp. 311-320 ◽  
Author(s):  
Emilio Lecona ◽  
Nieves Olmo ◽  
Javier Turnay ◽  
Angélica Santiago-Gómez ◽  
Isabel López de Silanes ◽  
...  
Keyword(s):  
Anion Exchanger ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Dependent Manner ◽  
High Affinity ◽  
Adenocarcinoma Cells ◽  
Butyrate Treatment ◽  
Human Colon Adenocarcinoma ◽  
Colon Adenocarcinoma Cells ◽  
Resistant Cells

Butyrate has antitumorigenic effects on colon cancer cells, inhibits cell growth and promotes differentiation and apoptosis. These effects depend on its intracellular concentration, which is regulated by its transport. We have analysed butyrate uptake kinetics in human colon adenocarcinoma cells sensitive to the apoptotic effects of butyrate (BCS-TC2, Caco-2 and HT-29), in butyrate-resistant cells (BCS-TC2.BR2) and in normal colonic cells (FHC). The properties of transport were analysed with structural analogues, specific inhibitors and different bicarbonate and sodium concentrations. Two carrier-mediated mechanisms were detected: a low-affinity/high-capacity (Km=109±16 mM in BCS-TC2 cells) anion exchanger and a high-affinity/low-capacity (Km=17.9±4.0 μM in BCS-TC2 cells) proton–monocarboxylate co-transporter that was energy-dependent and activated via PKCδ (protein kinase Cδ). All adenocarcinoma cells analysed express MCT (monocarboxylate transporter) 1, MCT4, ancillary protein CD147 and AE2 (anion exchanger 2). Silencing experiments show that MCT1, whose expression increases with butyrate treatment in butyrate-sensitive cells, plays a key role in high-affinity transport. Low-affinity uptake was mediated by a butyrate/bicarbonate antiporter along with a possible contribution of AE2 and MCT4. Butyrate treatment increased uptake in a time- and dose-dependent manner in butyrate-sensitive but not in butyrate-resistant cells. The two butyrate-uptake activities in human colon adenocarcinoma cells enable butyrate transport at different physiological conditions to maintain cell functionality. The high-affinity/low-capacity transport functions under low butyrate concentrations and may be relevant for the survival of carcinoma cells in tumour regions with low glucose and butyrate availability as well as for the normal physiology of colonocytes.

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