scholarly journals Phosphofructokinase 2 and glycolysis in HT29 human colon adenocarcinoma cell line. Regulation by insulin and phorbol esters

1990 ◽  
Vol 268 (2) ◽  
pp. 465-470 ◽  
Author(s):  
C Denis-Pouxviel ◽  
T Gauthier ◽  
D Daviaud ◽  
J C Murat
Keyword(s):  
Phorbol Esters ◽  
Lactate Production ◽  
Colon Adenocarcinoma ◽  
Glucose Consumption ◽  
Human Colon ◽  
Kinetic Properties ◽  
Dependent Manner ◽  
Glycerol Phosphate ◽  
Ht29 Cells ◽  
Long Term Treatment

Kinetic properties of phosphofructokinase 2 (PFK2) and regulation of glycolysis by phorbol 12-myristate 13-acetate (PMA) and insulin were investigated in highly glycolytic HT29 colon cancer cells. PFK2 was found to be inhibited by citrate and, to a lesser extent, by phosphoenolpyruvate and ADP, but to be insensitive to inhibition by sn-glycerol phosphate. From these kinetic data, PFK2 from HT29 cells appears different from the liver form, but resembles somewhat the heart isoenzyme. Fructose 2,6-bisphosphate (Fru-2,6-P2) levels, glucose consumption and lactate production are increased in a dose-dependent manner in HT29 cells treated with PMA or insulin. The increase in Fru-2,6-P2 can be related to an increase in the Vmax. of PFK2, persisting after the enzyme has been precipitated with poly(ethylene glycol), without change in the Km for fructose 6-phosphate. The most striking effects of PMA and insulin on Fru-2,6-P2 production are observed after long-term treatment (24 h) and are abolished by actinomycin, cycloheximide and puromycin, suggesting that protein synthesis is involved. Furthermore, the effects of insulin and PMA on glucose consumption, lactate production, Fru-2,6-P2 levels and PFK2 activity are additive, and the effect of insulin on Fru-2,6-P2 production is not altered by pre-treatment of the cells with the phorbol ester. This suggests that these effects are exerted by separate mechanisms.

scholarly journals Pyruvate Treatment Restores the Effectiveness of Chemotherapeutic Agents in Human Colon Adenocarcinoma and Pleural Mesothelioma Cells

2018 ◽  
Vol 19 (11) ◽  
pp. 3550 ◽  
Author(s):  
Eleonora Mungo ◽  
Loredana Bergandi ◽  
Iris Salaroglio ◽  
Sophie Doublier
Keyword(s):  
Multidrug Resistance ◽  
Cancer Cells ◽  
Colon Adenocarcinoma ◽  
Glucose Consumption ◽  
Mitochondrial Respiratory Chain ◽  
Human Colon ◽  
Chemotherapeutic Agents ◽  
Cross Resistance ◽  
Ht29 Cells ◽  
Human Colon Adenocarcinoma

Emerging evidence supports the idea that a dysfunction in cell metabolism could sustain a resistant phenotype in cancer cells. As the success of chemotherapeutic agents is often questioned by the occurrence of multidrug resistance (MDR), a multiple cross-resistance towards different anti-cancer drugs represent a major obstacle to cancer treatment. The present study has clarified the involvement of the carbon metabolites in a more aggressive tumor colon adenocarcinoma phenotype and in a chemoresistant mesothelioma, and the role of pyruvate treatment in the reversion of the potentially related resistance. For the first time, we have shown that human colon adenocarcinoma cells (HT29) and its chemoresistant counterpart (HT29-dx) displayed different carbon metabolism: HT29-dx cells had a higher glucose consumption compared to HT29 cells, whereas human malignant mesothelioma (HMM) cells showed a lower glucose consumption compared to HT29 cells, accompanied by a lower pyruvate production and, consequently, a higher production of lactate. When treated with pyruvate, both HT29-dx and HMM cells exhibited a re-established accumulation of doxorubicin and a lower survival ability, a decreased activity of multidrug resistance protein 1 (MRP1) and a restored mitochondrial respiratory chain function, improving the effectiveness of the chemotherapeutic agents in these resistant cancer cells.

Induction of G2/M Arrest and Apoptosis by the Methanol Extract of Typha orientalis in Human Colon Adenocarcinoma HT29 Cells

2013 ◽  
Vol 41 (4) ◽  
pp. 425-432 ◽  
Author(s):  
Soojung Jin ◽  
Seung-Geun Yun ◽  
You Na Oh ◽  
Ji-Young Lee ◽  
Hyun-jin Park ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Ht29 Cells ◽  
Typha Orientalis ◽  
Human Colon Adenocarcinoma

scholarly journals Safed Musli (Chlorophytum borivilianum L.) Callus-Mediated Biosynthesis of Silver Nanoparticles and Evaluation of their Antimicrobial Activity and Cytotoxicity against Human Colon Cancer Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Fengchang Huang ◽  
Yaxin Long ◽  
Qingqing Liang ◽  
Boregowda Purushotham ◽  
Mallappa Kumara Swamy ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Dependent Manner ◽  
Face Centered Cubic ◽  
Human Colon Cancer ◽  
Chlorophytum Borivilianum ◽  
The Face ◽  
Average Size ◽  
Safed Musli

With the advancement of nanobiotechnology, eco-friendly approaches of plant-mediated silver nanomaterial (AgNP) biosynthesis have become more attractive for biomedical applications. The present study is a report of biosynthesizing AgNPs using Chlorophytum borivilianum L. (Safed musli) callus extract as a novel source of reducing agent. AgNO3 solution challenged with the methanolic callus extract displayed a change in color from yellow to brown owing to the bioreduction reaction. Further, AgNPs were characterized by using UV–visible spectrophotometry, X-ray Diffraction (XRD), Atomic Force Microscopy (AFM), and Fourier Transform Infrared Spectroscopy (FTIR). UV–vis spectrum revealed the surface plasmon resonance property of AgNPs at around 450 nm. XRD pattern with typical peaks indicated the face-centered cubic nature of silver. AFM analysis confirmed the existence of spherical-shaped and well-dispersed AgNPs having an average size of 52.0 nm. Further, FTIR analysis confirmed the involvement of different phytoconstituents of the callus extract role in the process of bioreduction to form nanoparticles. The AgNPs were more efficient in inhibiting the tested pathogenic microbes, namely, Pseudomonas aeruginosa, Bacillus subtilis, Methicillin-resistant Escherichia coli, Staphylococcus aureus, and Candida albicans compared to callus extract. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay confirmed the cytotoxic property of AgNPs against human colon adenocarcinoma cell line (HT-29) in a dose-dependent manner. At higher concentrations of 500 μg/mL AgNPs, the cell viability was observed to be only 7% after 24 hours with IC50 value of 254 μg/mL. Therefore, these AgNPs clearly endorse the manifold potential to be used in various biomedical applications in the near future.

Synthesis and photobiological properties of novel silicon(IV) phthalocyanines axially modified by paracetamol and 4-hydroxyphenylacetamide

2009 ◽  
Vol 13 (12) ◽  
pp. 1227-1232 ◽  
Author(s):  
Jian-Dong Huang ◽  
Xiong-Jie Jiang ◽  
Xiao-Min Shen ◽  
Qing-Qing Tang
Keyword(s):  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Biological Properties ◽  
Ht29 Cells ◽  
Adenocarcinoma Cells ◽  
Photodynamic Activity ◽  
Lower Singlet ◽  
Very High ◽  
Colon Adenocarcinoma Cells

Two novel axial-disubstituted silicon(IV) phthalocyanines (compounds 1 and 2) have been prepared by introducing paracetamol (a common antipyretic analgesic) or its isomer 4-hydroxyphenylacetamide at the axial positions of silicon(IV) phthalocyanine, respectively. Their photophysical and biological properties have been examined. Both compounds are highly soluble and exhibit very similar absorption spectra in N, N-dimethylformamide, which is typical for non-aggregated phthalocyanines. Both compounds are photocytotoxic against HT29 human colon adenocarcinoma cells. Compound 2 shows a very high in vitro photodynamic activity, with the IC50 value down to 15 nM. In contrast, compound 1 exhibits a much lower in vitro photodynamic activity toward HT29 cells, which can be attributed to its higher aggregating trend in the biological medium and lower singlet oxygen quantum yield.

scholarly journals Lack of integrin α-chain endoproteolytic cleavage in furin-deficient human colon adenocarcinoma cells LoVo

1996 ◽  
Vol 317 (3) ◽  
pp. 803-809 ◽  
Author(s):  
Maxime LEHMANN ◽  
Véronique RIGOT ◽  
Nabil G. SEIDAH ◽  
Jacques MARVALDI ◽  
Jean-Claude LISSITZKY
Keyword(s):  
Present Report ◽  
Colon Adenocarcinoma ◽  
Complete Removal ◽  
Human Colon ◽  
Competent Cell ◽  
Integrin Subunit ◽  
Furin Cleavage ◽  
Ht29 Cells ◽  
Endoproteolytic Cleavage ◽  
Lovo Cells

In the present report the biosynthesis of the integrin α-chains endowed with constitutive endoproteolytic cleavage was evaluated in LoVo cells where furin, a subtilisin-like convertase involved in post-translational endoproteolytic processing, is not functional. It was found that cell-surface α3, α6 and αv subunits were not processed endoproteolytically into heavy and light chains as they were in HT29-D4 cells, a furin-competent cell line. Complete removal of N-linked oligosaccharides and pulse–chase experiments confirmed that the cleavage of the α6 integrin subunit occurring 45 min after translation in HT29 cells did not take place in LoVo cells. Apart from cleavage deficiency, α6 subunit glycosylation, association with β4 subunits and targeting to the plasma membrane seemed comparable in LoVo and HT29 cells. The pro-α6 and the pro-α3 subunits immunopurified from LoVo cells were highly sensitive to endoproteolysis by recombinant furin. Furin cleavage was calcium dependent and resulted in the conversion of the 140 kDa pro-α6 into a 120 kDa heavy chain. These results suggest strongly that furin is involved in the endoproteolytic processing of cleavable integrin α subunits.

Expression of IGF-II and IGF binding proteins in differentiating human intestinal Caco-2 cells

1995 ◽  
Vol 269 (5) ◽  
pp. E804-E813 ◽  
Author(s):  
Y. Zhang ◽  
D. A. Wick ◽  
B. Seetharam ◽  
N. M. Dahms
Keyword(s):  
Conditioned Medium ◽  
Binding Proteins ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Metabolic Effects ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Proliferating Cells ◽  
Igf Binding Proteins ◽  
Igf Binding

The mitogenic and metabolic effects of insulin-like growth factor-II (IGF-II) can be modulated by six distinct IGF binding proteins (IGFBPs). As a first step toward understanding the role of IGFs and their binding proteins in intestinal epithelial cell differentiation, the expression of IGF-II and IGFBPs was characterized in the human colon adenocarcinoma Caco-2 cell line. Northern blot analysis revealed two IGF-II transcripts of 5.4 and 4.5 kb, and ribonuclease protection assays indicated that IGF-II mRNA levels are regulated during Caco-2 differentiation. A specific radioimmunoassay detected IGF-II in serum-free conditioned medium, the level of which was three- to fivefold higher in proliferating cells than in differentiated cells. Immunoprecipitation and ligand blot analyses of conditioned medium demonstrated that IGFBP-2, IGFBP-3, IGFBP-4, and IGFBP-6 are synthesized by Caco-2 cells, with IGFBP-2 and IGFBP-4 being the major IGFBPs secreted, and that the levels of IGFBP-2 and IGFBP-6 decreased as differentiation proceeded. These results indicate that the expression of IGF-II, IGFBP-2, and IGFBP-6 is regulated in a differentiation-dependent manner in Caco-2 cells.

scholarly journals The Interplay between Fe3O4 Superparamagnetic Nanoparticles, Sodium Butyrate, and Folic Acid for Intracellular Transport

2020 ◽  
Vol 21 (22) ◽  
pp. 8473
Author(s):  
Maria Teresa Cambria ◽  
Giusy Villaggio ◽  
Samuele Laudani ◽  
Luca Pulvirenti ◽  
Concetta Federico ◽  
...  
Keyword(s):  
Folic Acid ◽  
Sodium Butyrate ◽  
Intracellular Transport ◽  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Superparamagnetic Nanoparticles ◽  
High Dose ◽  
Dependent Manner ◽  
The Difference ◽  
Surface Modified

Combined treatments which use nanoparticles and drugs could be a synergistic strategy for the treatment of a variety of cancers to overcome drug resistance, low efficacy, and high-dose-induced systemic toxicity. In this study, the effects on human colon adenocarcinoma cells of surface modified Fe3O4 magnetic nanoparticles (MNPs) in combination with sodium butyrate (NaBu), added as a free formulation, were examined demonstrating that the co-delivery produced a cytotoxic effect on malignant cells. Two different MNP coatings were investigated: a simple polyethylene glycol (PEG) layer and a mixed folic acid (FA) and PEG layer. Our results demonstrated that MNPs with FA (FA-PEG@MNPs) have a better cellular uptake than the ones without FA (PEG@MNPs), probably due to the presence of folate that acts as an activator of folate receptors (FRs) expression. However, in the presence of NaBu, the difference between the two types of MNPs was reduced. These similar behaviors for both MNPs likely occurred because of the differentiation induced by butyrate that increases the uptake of ferromagnetic nanoparticles. Moreover, we observed a strong decrease of cell viability in a NaBu dose-dependent manner. Taking into account these results, the cooperation of multifunctional MNPs with NaBu, taking into consideration the particular cancer-cell properties, can be a valuable tool for future cancer treatment.

Regulation of mesangial cell hexokinase activity by PKC and the classic MAPK pathway

1999 ◽  
Vol 277 (5) ◽  
pp. F742-F749 ◽  
Author(s):  
R. Brooks Robey ◽  
Jianfei Ma ◽  
Anna V. P. Santos
Keyword(s):  
Time Course ◽  
Phorbol Esters ◽  
Mesangial Cell ◽  
De Novo ◽  
Mesangial Cells ◽  
Mapk Pathway ◽  
Lactate Production ◽  
Cell Types ◽  
Functional Coupling ◽  
Dependent Manner

Phorbol esters increase glucose (Glc) uptake and utilization in a variety of cell types, and, in some cells, these changes have been attributed to increased Glc phosphorylation and better functional coupling of hexokinases (HKs) to facilitative Glc transporters. Phorbol esters are potent mesangial cell mitogens, but their effects on HK-catalyzed Glc phosphorylation and metabolism are unknown. When examined in murine mesangial cells, active, but not inactive, phorbol esters increased HK activity in a time- and dose-dependent manner. Maximal induction of HK activity at 12–24 h was accompanied by parallel increases in both Glc utilization and lactate production and was blocked by the specific MEK1/2 inhibitor PD-98059 (IC50∼3 μM). This effect involved early activation of protein kinase C (PKC), MEK1/2, and ERK1/2, and the prolonged time course of subsequent HK induction was attributable, in part, to requirements for ongoing gene transcription and de novo protein synthesis. Mesangial cell HK activity thus exhibits novel regulatory behavior involving both PKC and classic MAPK pathway activation, suggesting specific mechanisms whereby PKC activation may influence Glc metabolism.

scholarly journals Glutathione conjugation of chlorambucil: measurement and modulation by plant polyphenols

1997 ◽  
Vol 325 (2) ◽  
pp. 417-422 ◽  
Author(s):  
Kai ZHANG ◽  
Kim Ping WONG
Keyword(s):  
Colon Adenocarcinoma ◽  
Human Colon ◽  
Thymidine Uptake ◽  
Dependent Manner ◽  
Glutathione S Transferase ◽  
Plant Polyphenols ◽  
Simultaneous Exposure ◽  
Adenocarcinoma Cells ◽  
Combined Action ◽  
Dose Dependent

Chlorambucil (CMB), an anticancer drug, was cytotoxic at concentrations of 5–20 μM to human colon adenocarcinoma cells. It inhibited [14C]thymidine uptake in a dose-dependent manner. Both effects were potentiated by simultaneous exposure of the cells to 10 μM plant polyphenols. In an attempt to explain the possible mechanism of action of the polyphenols in relation to these observations, an HPLC-radiometric method was developed to measure the conjugation of CMB with glutathione in these cells and to monitor the export of monochloromonoglutathionyl CMB (MG-CMB), its main glutathione conjugate. At micromolar concentrations, five polyphenols, namely quercetin, butein, tannic acid, 2′-hydroxychalcone and morin, inhibited the efflux of CMB significantly; an inhibition of 40% was observed with 10 μM quercetin. The glutathione S-transferase (GST) activity of the cancer cells, measured with 1-chloro-2,4-dinitrobenzene, was also inhibited by the polyphenols. Their combined action on GST and on the efflux of MG-CMB conjugate could provide an enhanced positive modulation of sensitivity of the tumour cells to CMB.

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