scholarly journals Protective Effects of Colomast®, a New Formulation of Adelmidrol and Sodium Hyaluronate, in a Mouse Model of Acute Restraint Stress

2020 ◽  
Vol 21 (21) ◽  
pp. 8136
Author(s):  
Ramona D’Amico ◽  
Rosalba Siracusa ◽  
Roberta Fusco ◽  
Marika Cordaro ◽  
Tiziana Genovese ◽  
...  
Keyword(s):  
Restraint Stress ◽  
Cell Activation ◽  
Sodium Hyaluronate ◽  
Neutrophil Infiltration ◽  
Mast Cell Activation ◽  
Adhesive Tape ◽  
Protective Effects ◽  
Depression And Anxiety ◽  
Acute Restraint Stress ◽  
Apoptosis Pathways

Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones. Stress is also known to be a risk factor for the development of depression and anxiety. However, until today there are no suitable therapies for treating of stress. The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress. Acute restraint stress (ARS) was induced in mice by fixation for 2 h of the four extremities with an adhesive tape and Colomast® (20 mg/kg) was administered by oral gavage 30 min before the immobilization. Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction. Moreover, Colomast® was able to restore tight junction in both ileum and hippocampus and cortex. Additionally, we demonstrated that Colomast® ameliorated depression and anxiety-related behaviours, and modulate inflammatory and apoptosis pathways also in brain after ARS induction. In conclusion, our results suggest Colomast® to be a potential approach to ARS.

α-(phenylselanyl) acetophenone abolishes acute restraint stress induced-comorbid pain, depression and anxiety-related behaviors in mice

2018 ◽  
Vol 120 ◽  
pp. 112-120 ◽  
Author(s):  
Fernanda Severo Sabedra Sousa ◽  
Paloma Taborda Birmann ◽  
Renata Balaguez ◽  
Diego Alves ◽  
César Augusto Brüning ◽  
...  
Keyword(s):  
Restraint Stress ◽  
Depression And Anxiety ◽  
Acute Restraint Stress

scholarly journals Protein Disulfide Isomerases Regulate IgE-Mediated Mast Cell Responses and Their Inhibition Confers Protective Effects During Food Allergy

2020 ◽  
Vol 11 ◽  
Author(s):  
Dylan Krajewski ◽  
Stephanie H. Polukort ◽  
Justine Gelzinis ◽  
Jeffrey Rovatti ◽  
Edwin Kaczenski ◽  
...  
Keyword(s):  
Mast Cell ◽  
Food Allergy ◽  
Mast Cells ◽  
Cell Activation ◽  
Cell Activity ◽  
Mast Cell Activation ◽  
Protective Effects ◽  
Ige Mediated ◽  
Protein Disulfide

The thiol isomerase, protein disulfide isomerase (PDI), plays important intracellular roles during protein folding, maintaining cellular function and viability. Recent studies suggest novel roles for extracellular cell surface PDI in enhancing cellular activation and promoting their function. Moreover, a number of food-derived substances have been shown to regulate cellular PDI activity and alter disease progression. We hypothesized that PDI may have similar roles during mast cell-mediated allergic responses and examined its effects on IgE-induced mast cell activity during cell culture and food allergy. Mast cells were activated via IgE and antigen and the effects of PDI inhibition on mast cell activation were assessed. The effects of PDI blockade in vivo were examined by treating mice with the irreversible PDI inhibitor, PACMA-31, in an ovalbumin-induced model of food allergy. The role of dietary PDI modulators was investigated using various dietary compounds including curcumin and quercetin-3-rutinoside (rutin). PDI expression was observed on resting mast cell surfaces, intracellularly, and in the intestines of allergic mice. Furthermore, enhanced secretion of extracellular PDI was observed on mast cell membranes during IgE and antigen activation. Insulin turbidimetric assays demonstrated that curcumin is a potent PDI inhibitor and pre-treatment of mast cells with curcumin or established PDI inhibitors such as bacitracin, rutin or PACMA-31, resulted in the suppression of IgE-mediated activation and the secretion of various cytokines. This was accompanied by decreased mast cell proliferation, FcεRI expression, and mast cell degranulation. Similarly, treatment of allergic BALB/c mice with PACMA-31 attenuated the development of food allergy resulting in decreased allergic diarrhea, mast cell activation, and fewer intestinal mast cells. The production of TH2-specific cytokines was also suppressed. Our observations suggest that PDI catalytic activity is essential in the regulation of mast cell activation, and that its blockade may benefit patients with allergic inflammation.

Costunolide ameliorates intestinal dysfunction and depressive behaviour in mice with stress-induced irritable bowel syndrome via colonic mast cell activation and central 5-hydroxytryptamine metabolism

2021 ◽  
Author(s):  
Xi Li ◽  
Qingqing Liu ◽  
Jiaoyan Yu ◽  
Ruitao Zhang ◽  
Ting Sun ◽  
...  
Keyword(s):  
Mast Cell ◽  
Irritable Bowel Syndrome ◽  
High Risk ◽  
Bowel Disease ◽  
Cell Activation ◽  
Mast Cell Activation ◽  
Depression And Anxiety ◽  
Functional Bowel Disease ◽  
Irritable Bowel ◽  
The Brain

Irritable bowel syndrome (IBS) is a common chronic functional bowel disease, associated with a high risk of depression and anxiety. The brain–gut axis plays an important role in the pathophysiological...

Acute restraint stress has a similar effect on visceral sensitivity in different types of maternal deprivation in rats

2001 ◽  
Vol 120 (5) ◽  
pp. A715-A715
Author(s):  
A ROSZTOCZY ◽  
S BRADESI ◽  
C BEAUFRAND ◽  
J FIORAMONTI ◽  
T WITTMANN ◽  
...  
Keyword(s):  
Restraint Stress ◽  
Maternal Deprivation ◽  
Visceral Sensitivity ◽  
Acute Restraint Stress ◽  
Different Types
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