scholarly journals Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering

2020 ◽  
Vol 21 (18) ◽  
pp. 6544 ◽  
Author(s):  
Neha Kaushik ◽  
Linh Nhat Nguyen ◽  
June Hyun Kim ◽  
Eun Ha Choi ◽  
Nagendra Kumar Kaushik
Keyword(s):  
Tissue Engineering ◽  
Biomedical Applications ◽  
Cell Attachment ◽  
Cell Types ◽  
Bioactive Molecules ◽  
Cellular Interaction ◽  
Biomimetic Mineralization ◽  
Adhesive Properties ◽  
Implant Materials ◽  
Defect Repair

In the field of tissue engineering, there are several issues to consider when designing biomaterials for implants, including cellular interaction, good biocompatibility, and biochemical activity. Biomimetic mineralization has gained considerable attention as an emerging approach for the synthesis of biocompatible materials with complex shapes, categorized organization, controlled shape, and size in aqueous environments. Understanding biomineralization strategies could enhance opportunities for novel biomimetic mineralization approaches. In this regard, mussel-inspired biomaterials have recently attracted many researchers due to appealing features, such as strong adhesive properties on moist surfaces, improved cell adhesion, and immobilization of bioactive molecules via catechol chemistry. This molecular designed approach has been a key point in combining new functionalities into accessible biomaterials for biomedical applications. Polydopamine (PDA) has emerged as a promising material for biomaterial functionalization, considering its simple molecular structure, independence of target materials, cell interactions for adhesion, and robust reactivity for resulting functionalization. In this review, we highlight the strategies for using PDA to induce the biomineralization of hydroxyapatite (HA) on the surface of various implant materials with good mechanical strength and corrosion resistance. We also discuss the interactions between the PDA-HA coating, and several cell types that are intricate in many biomedical applications, involving bone defect repair, bone regeneration, cell attachment, and antibacterial activity.

scholarly journals Tissue engineering of urethra: Systematic review of recent literature

2017 ◽  
Vol 242 (18) ◽  
pp. 1772-1785 ◽  
Author(s):  
Stanislav Žiaran ◽  
Martina Galambošová ◽  
L'uboš Danišovič
Keyword(s):  
Systematic Review ◽  
Tissue Engineering ◽  
Clinical Trials ◽  
Animal Studies ◽  
Recent Literature ◽  
Cell Attachment ◽  
Experimental Studies ◽  
Clinical Results ◽  
Bioactive Molecules ◽  
Urethral Reconstruction

The purpose of this article was to perform a systematic review of the recent literature on urethral tissue engineering. A total of 31 articles describing the use of tissue engineering for urethra reconstruction were included. The obtained results were discussed in three groups: cells, scaffolds, and clinical results of urethral reconstructions using these components. Stem cells of different origin were used in many experimental studies, but only autologous urothelial cells, fibroblasts, and keratinocytes were applied in clinical trials. Natural and synthetic scaffolds were studied in the context of urethral tissue engineering. The main advantage of synthetic ones is the fact that they can be obtained in unlimited amount and modified by different techniques, but scaffolds of natural origin normally contain chemical groups and bioactive proteins which increase the cell attachment and may promote the cell proliferation and differentiation. The most promising are smart scaffolds delivering different bioactive molecules or those that can be tubularized. In two clinical trials, only onlay-fashioned transplants were used for urethral reconstruction. However, the very promising results were obtained from animal studies where tubularized scaffolds, both non-seeded and cell-seeded, were applied. Impact statement The main goal of this article was to perform a systematic review of the recent literature on urethral tissue engineering. It summarizes the most recent information about cells, seeded or non-seeded scaffolds and clinical application with respect to regeneration of urethra.

scholarly journals Development of 3D Bioactive Nanocomposite Scaffolds Made from Gelatin and Nano Bioactive Glass for Biomedical Applications

2010 ◽  
Vol 19 (2) ◽  
pp. 096369351001900 ◽  
Author(s):  
M. Mozafari ◽  
F. Moztarzadeh ◽  
M. Rabiee ◽  
M. Azami ◽  
N. Nezafati ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Biomedical Applications ◽  
Cell Attachment ◽  
Tissue Engineering Scaffolds ◽  
Study Results ◽  
Nanocomposite Scaffold ◽  
Nanocomposite Scaffolds ◽  
First Time

In this research, macroporous, mechanically competent and bioactive nanocomposite scaffolds have been fabricated from cross-linked gelatine (Gel) and nano bioactive glass (nBG) through layer solvent casting combined with freeze-drying and lamination techniques. This study has developed a new composition to produce a new bioactive nanocomposite which is porous with interconnected microstructure, pore sizes are 200-500 μm, porosity are 72%-86%. Also, we have reported formation of chemical bonds between nBG and Gel for the first time. Finally, the in vitro cytocompatability of the scaffolds was assessed using MTT assay and cell attachment study. Results indicated no sign of toxicity and cells found to be attached to the pore walls offered by the scaffolds. These results suggested that the developed nanocomposite scaffold possess the prerequisites for bone tissue engineering scaffolds and it can be used for tissue engineering applications.

Choline phosphate functionalized surface: protein-resistant but cell-adhesive zwitterionic surface potential for tissue engineering

2015 ◽  
Vol 51 (3) ◽  
pp. 487-490 ◽  
Author(s):  
Xingyu Chen ◽  
Tianchan Chen ◽  
Zaifu Lin ◽  
Xian'e Li ◽  
Wei Wu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Surface Potential ◽  
Biomedical Applications ◽  
Surface Protein ◽  
Adhesive Properties ◽  
Cell Adhesive ◽  
Choline Phosphate ◽  
Protein Resistant ◽  
Functionalized Surface

A choline phosphate (CP) modified zwitterionic surface is prepared with both protein-resistant and cell-adhesive properties, exhibiting great potential for biomedical applications such as tissue engineering.

scholarly journals Cell attachment to thrombospondin: the role of ARG-GLY-ASP, calcium, and integrin receptors.

1988 ◽  
Vol 107 (6) ◽  
pp. 2351-2361 ◽  
Author(s):  
J Lawler ◽  
R Weinstein ◽  
R O Hynes
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Cell Attachment ◽  
Solid Phase ◽  
Rat Kidney ◽  
Muscle Cells ◽  
Cell Types ◽  
Adhesive Properties ◽  
Adhesive Proteins

Thrombospondin is a 420,000-D glycoprotein that has recently been shown to have several properties in common with the members of a class of adhesive proteins. To characterize further the adhesive properties of thrombospondin, we have studied its ability to support cell attachment. Thrombospondin adsorbed to plastic dishes supports the attachment of human endothelial and smooth muscle cells and the monocyte-like cell line (U937) as well as normal rat kidney cells. The majority of attached cells do not spread on the solid-phase thrombospondin. The attachment of all four cell types to thrombospondin is abolished if the assay is performed in the presence of EGTA, although the cells still attach to fibronectin. If thrombospondin is adsorbed to the dishes in the presence of EGTA and then washed with buffer containing calcium before addition of the cells, attachment is still markedly inhibited, indicating that calcium affects the conformation and function of thrombospondin. Attachment of all four cell types is also markedly inhibited by the synthetic peptides gly-arg-gly-asp-ser-pro (GRG-DSP) and gly-arg-gly-asp-ala-cys (GRGDAC) but not by the control peptide gly-arg-gly-glu-ser-pro (GRG-ESP). Affinity chromatography of n-octylglucoside extracts of surface-labeled endothelial cells or smooth muscle cells on thrombospondin-Sepharose and GRG-DSP-Affigel columns was used to identify an integrin complex related to glycoprotein IIb-IIIa as an RGD-dependent receptor for thrombospondin. In addition, a monoclonal antibody (LM609) that blocks attachment of endothelial cells to vitronectin, fibrinogen, and von Willebrand factor also inhibits attachment of endothelial cells to thrombospondin. These data indicate that the attachment of cells to thrombospondin is mediated by RGD and calcium-dependent mechanisms and is consistent with the hypothesis that the GRGDAC sequence in thrombospondin is a site for interaction with an integrin receptor of the beta 3 subclass.

scholarly journals Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress

2021 ◽  
Vol 7 (2) ◽  
Author(s):  
Sara Cristina Pedroza-González ◽  
Marisela Rodriguez-Salvador ◽  
Baruc Emet Pérez Benítez ◽  
Mario Moisés Alvarez ◽  
Grissel Trujillo-de Santiago
Keyword(s):  
Biomedical Applications ◽  
Cell Attachment ◽  
Cell Types ◽  
Scientometric Analysis ◽  
Binding Motif ◽  
3D Bioprinting ◽  
Cell Binding ◽  
Biological Performance ◽  
The Cost

This scientometric analysis of 393 original papers published from January 2000 to June 2019 describes the development and use of bioinks for 3D bioprinting. The main trends for bioink applications and the primary considerations guiding the selection and design of current bioink components (i.e., cell types, hydrogels, and additives) were reviewed. The cost, availability, practicality, and basic biological considerations (e.g., cytocompatibility and cell attachment) are the most popular parameters guiding bioink use and development. Today, extrusion bioprinting is the most widely used bioprinting technique. The most reported use of bioinks is the generic characterization of bioink formulations or bioprinting technologies (32%), followed by cartilage bioprinting applications (16%). Similarly, the cell-type choice is mostly generic, as cells are typically used as models to assess bioink formulations or new bioprinting methodologies rather than to fabricate specific tissues. The cell-binding motif arginine-glycine-aspartate is the most common bioink additive. Many articles reported the development of advanced functional bioinks for specific biomedical applications; however, most bioinks remain the basic compositions that meet the simple criteria: Manufacturability and essential biological performance. Alginate and gelatin methacryloyl are the most popular hydrogels that meet these criteria. Our analysis suggests that present-day bioinks still represent a stage of emergence of bioprinting technology.

scholarly journals Marine Collagen as A Promising Biomaterial for Biomedical Applications

Marine Drugs ◽  
2019 ◽  
Vol 17 (8) ◽  
pp. 467 ◽  
Author(s):  
Ye-Seon Lim ◽  
Ye-Jin Ok ◽  
Seon-Yeong Hwang ◽  
Jong-Young Kwak ◽  
Sik Yoon
Keyword(s):  
Tissue Engineering ◽  
Biomedical Applications ◽  
Water Solubility ◽  
Cell Attachment ◽  
3D Structure ◽  
Biochemical Properties ◽  
Production Costs ◽  
Great Promise ◽  
Metabolic Disturbances ◽  
Obesity And Diabetes

This review focuses on the expanding role of marine collagen (MC)-based scaffolds for biomedical applications. A scaffold—a three-dimensional (3D) structure fabricated from biomaterials—is a key supporting element for cell attachment, growth, and maintenance in 3D cell culture and tissue engineering. The mechanical and biological properties of the scaffolds influence cell morphology, behavior, and function. MC, collagen derived from marine organisms, offers advantages over mammalian collagen due to its biocompatibility, biodegradability, easy extractability, water solubility, safety, low immunogenicity, and low production costs. In recent years, the use of MC as an increasingly valuable scaffold biomaterial has drawn considerable attention from biomedical researchers. The characteristics, isolation, physical, and biochemical properties of MC are discussed as an understanding of MC in optimizing the subsequent modification and the chemistries behind important tissue engineering applications. The latest technologies behind scaffold processing are assessed and the biomedical applications of MC and MC-based scaffolds, including tissue engineering and regeneration, wound dressing, drug delivery, and therapeutic approach for diseases, especially those associated with metabolic disturbances such as obesity and diabetes, are discussed. Despite all the challenges, MC holds great promise as a biomaterial for developing medical products and therapeutics.

scholarly journals Chitosan Applications Used in Medical Therapy of Tissue Regeneration

2016 ◽  
Vol 2 (2) ◽  
pp. 271
Author(s):  
Alef Mustafa ◽  
Ana Maria Ionescu ◽  
Melat Cherim ◽  
Rodica Sîrbu
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Biomedical Applications ◽  
Vascular Tissue ◽  
Bulk Material ◽  
Cell Types ◽  
Biological Molecules ◽  
Great Promise ◽  
Nucleus Pulposus Cells ◽  
Biological Specificity

Chitosan is an unique natural biopolymer that has great potential in tissue engineering applications and over the past several decades, it has emerged as a promising biomaterial for biomedical applications. Due to its various properties such as controllable biodegradability, biocompatibility, antimicrobial activity and functionalizability, chitosan can be used to form chitosan-based scaffolds and in different scaffold fabrication techniques. Over the years a great number of studies have been performed to evaluate the cytocompatibility of chitosan using a variety off cell types such as osteoblasts, chondrocytes, fibroblasts, nucleus pulposus cells, neutral and endothelial cells. It was shown that chitosan is biocompatible with these cell types and has the potential to be used for bone, cartilage, skin, intervertebral disc, ligament and tendon, and nerve and vascular tissue engineering. The flexibility of the processing conditions of chitosan aids in the fabrication of versatile substrates as scaffolds for tissue regeneration or carriers for biological molecules. It is critical to synthesize medical grade chitosan materials with controllable structure and properties that will allow the development of chitosan-based medical devices and it is beneficial to chemically design chitosan derivatives with molecular and biological specificity through bulk material modification. Despite all the challenges, chitosan holds great promise as a biomaterial for developing medical products and medical therapies.

Cell-derived decellularized extracellular matrix scaffolds for articular cartilage repair

2020 ◽  
pp. 039139882095386
Author(s):  
Wenrun Zhu ◽  
Lu Cao ◽  
Chunfeng Song ◽  
Zhiying Pang ◽  
Haochen Jiang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Articular Cartilage ◽  
Cartilage Repair ◽  
Cell Attachment ◽  
Tissue Growth ◽  
Bioactive Molecules ◽  
Cartilage Tissue ◽  
Articular Cartilage Repair ◽  
Decellularized Extracellular Matrix

Articular cartilage repair remains a great clinical challenge. Tissue engineering approaches based on decellularized extracellular matrix (dECM) scaffolds show promise for facilitating articular cartilage repair. Traditional regenerative approaches currently used in clinical practice, such as microfracture, mosaicplasty, and autologous chondrocyte implantation, can improve cartilage repair and show therapeutic effect to some degree; however, the long-term curative effect is suboptimal. As dECM prepared by proper decellularization procedures is a biodegradable material, which provides space for regeneration tissue growth, possesses low immunogenicity, and retains most of its bioactive molecules that maintain tissue homeostasis and facilitate tissue repair, dECM scaffolds may provide a biomimetic microenvironment promoting cell attachment, proliferation, and chondrogenic differentiation. Currently, cell-derived dECM scaffolds have become a research hotspot in the field of cartilage tissue engineering, as ECM derived from cells cultured in vitro has many advantages compared with native cartilage ECM. This review describes cell types used to secrete ECM, methods of inducing cells to secrete cartilage-like ECM and decellularization methods to prepare cell-derived dECM. The potential mechanism of dECM scaffolds on cartilage repair, methods for improving the mechanical strength of cell-derived dECM scaffolds, and future perspectives on cell-derived dECM scaffolds are also discussed in this review.

scholarly journals Chitosan Applications Used in Medical Therapy of Tissue Regeneration

2016 ◽  
Vol 4 (2) ◽  
pp. 271 ◽  
Author(s):  
Alef Mustafa ◽  
Ana Maria Ionescu ◽  
Melat Cherim ◽  
Rodica Sîrbu
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Biomedical Applications ◽  
Vascular Tissue ◽  
Bulk Material ◽  
Cell Types ◽  
Biological Molecules ◽  
Great Promise ◽  
Nucleus Pulposus Cells ◽  
Biological Specificity

Chitosan is an unique natural biopolymer that has great potential in tissue engineering applications and over the past several decades, it has emerged as a promising biomaterial for biomedical applications. Due to its various properties such as controllable biodegradability, biocompatibility, antimicrobial activity and functionalizability, chitosan can be used to form chitosan-based scaffolds and in different scaffold fabrication techniques. Over the years a great number of studies have been performed to evaluate the cytocompatibility of chitosan using a variety off cell types such as osteoblasts, chondrocytes, fibroblasts, nucleus pulposus cells, neutral and endothelial cells. It was shown that chitosan is biocompatible with these cell types and has the potential to be used for bone, cartilage, skin, intervertebral disc, ligament and tendon, and nerve and vascular tissue engineering. The flexibility of the processing conditions of chitosan aids in the fabrication of versatile substrates as scaffolds for tissue regeneration or carriers for biological molecules. It is critical to synthesize medical grade chitosan materials with controllable structure and properties that will allow the development of chitosan-based medical devices and it is beneficial to chemically design chitosan derivatives with molecular and biological specificity through bulk material modification. Despite all the challenges, chitosan holds great promise as a biomaterial for developing medical products and medical therapies.

Scaffolds for hand tissue engineering: the importance of surface topography

2015 ◽  
Vol 40 (9) ◽  
pp. 973-985 ◽  
Author(s):  
E. Kloczko ◽  
D. Nikkhah ◽  
L. Yildirimer
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Cell Types ◽  
Specific Cell ◽  
Complex Structures ◽  
Research Focus ◽  
Functional Regeneration ◽  
Fully Functioning ◽  
Extracellular Environment

Tissue engineering is believed to have great potential for the reconstruction of the hand after trauma, congenital absence and tumours. Due to the presence of multiple distinct tissue types, which together function in a precisely orchestrated fashion, the hand counts among the most complex structures to regenerate. As yet the achievements have been limited. More recently, the focus has shifted towards scaffolds, which provide a three-dimensional framework to mimic the natural extracellular environment for specific cell types. In particular their surface structures (or topographies) have become a key research focus to enhance tissue-specific cell attachment and growth into fully functioning units. This article reviews the current understanding in hand tissue engineering before focusing on the potential for scaffold topographical features on micro- and nanometre scales to achieve better functional regeneration of individual and composite tissues.

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