scholarly journals Existence of SARS-CoV-2 Entry Molecules in the Oral Cavity

2020 ◽  
Vol 21 (17) ◽  
pp. 6000 ◽  
Author(s):  
Wakako Sakaguchi ◽  
Nobuhisa Kubota ◽  
Tomoko Shimizu ◽  
Juri Saruta ◽  
Shinya Fuchida ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Protease Inhibitors ◽  
Viral Entry ◽  
Squamous Epithelium ◽  
Cultured Cells ◽  
Lower Layer ◽  
Taste Bud ◽  
Tongue Coating ◽  
Dorsal Tongue ◽  
Stratified Squamous Epithelium

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) receptor, angiotensin-converting enzyme 2 (ACE2), transmembrane protease serine 2 (TMPRSS2), and furin, which promote entry of the virus into the host cell, have been identified as determinants of SARS-CoV-2 infection. Dorsal tongue and gingiva, saliva, and tongue coating samples were examined to determine the presence of these molecules in the oral cavity. Immunohistochemical analyses showed that ACE2 was expressed in the stratified squamous epithelium of the dorsal tongue and gingiva. TMPRSS2 was strongly expressed in stratified squamous epithelium in the keratinized surface layer and detected in the saliva and tongue coating samples via Western blot. Furin was localized mainly in the lower layer of stratified squamous epithelium and detected in the saliva but not tongue coating. ACE2, TMPRSS2, and furin mRNA expression was observed in taste bud-derived cultured cells, which was similar to the immunofluorescence observations. These data showed that essential molecules for SARS-CoV-2 infection were abundant in the oral cavity. However, the database analysis showed that saliva also contains many protease inhibitors. Therefore, although the oral cavity may be the entry route for SARS-CoV-2, other factors including protease inhibitors in the saliva that inhibit viral entry should be considered.

scholarly journals Extensive Epidermoid Cyst and Breathing Difficulty

2015 ◽  
Vol 2015 ◽  
pp. 1-4
Author(s):  
Ciro Dantas Soares ◽  
Alberto Costa Gurgel ◽  
Francisco de Assis de Souza Júnior ◽  
Samila Neres de Oliveira ◽  
Maria Goretti Freire de Carvalho ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Oral Cavity ◽  
Epidermoid Cyst ◽  
Surgical Planning ◽  
Squamous Epithelium ◽  
Functional Limitations ◽  
Lingual Thyroid ◽  
Cystic Lesions ◽  
Breathing Difficulty ◽  
Stratified Squamous Epithelium

Epidermoid cysts are common cystic lesions in the skin, ovaries, and testicles, but their occurrence in the oral cavity is uncommon. They consist of cysts delimited by a fibrous capsule without cutaneous annexes and are lined by stratified squamous epithelium. The differential diagnosis includes ranula, dermoid cysts, and lingual thyroid. Despite their benign presentation, these cysts can cause functional limitations, requiring special clinical attention for extensive lesions located in regions that preserve vital structures. This paper aims to report a case of epidermoid cyst in patient with swallowing and breathing difficulty, highlighting the clinical and surgical planning.

scholarly journals Immunolocalization of Surfactant Proteins SP-A, SP-B, SP-C, and SP-D in Infantile Labial Glands and Mucosa

2018 ◽  
Vol 66 (7) ◽  
pp. 531-538
Author(s):  
Mechthild Stoeckelhuber ◽  
Friedrich Feuerhake ◽  
Christoph Schmitz ◽  
Klaus-Dietrich Wolff ◽  
Marco R. Kesting
Keyword(s):  
Oral Cavity ◽  
Squamous Epithelium ◽  
Surfactant Proteins ◽  
Positive Staining ◽  
Oral Environment ◽  
Cell Layers ◽  
Stratified Squamous Epithelium ◽  
Glandular Structures ◽  
First Time ◽  
Labial Glands

Surfactant proteins in different glandular structures of the oral cavity display antimicrobial activity for protection of invading microorganisms. Moreover, they are involved in lowering liquid tension in fluids and facilitate secretion flows. Numerous investigations for studying the occurrence of surfactant proteins in glandular tissues were performed using different methods. In the oral cavity, minor salivary glands secrete saliva continuously for the maintenance of a healthy oral environment. For the first time, we could show that infantile labial glands show expression of the surfactant proteins (SP) SP-A, SP-B, SP-C, and SP-D in acinar cells and the duct system in different intensities. The stratified squamous epithelium of the oral mucosa revealed positive staining for SPs in various cell layers.

scholarly journals Verrucous carcinoma and squamous cell papilloma of the oral cavity: Report of two cases and review of literature

2015 ◽  
Vol 09 (03) ◽  
pp. 453-456 ◽  
Author(s):  
Hilal Alan ◽  
Serkan Agacayak ◽  
Gulten Kavak ◽  
Ayse Ozcan
Keyword(s):  
Oral Cavity ◽  
Squamous Cell ◽  
Squamous Epithelium ◽  
Verrucous Carcinoma ◽  
Review Of Literature ◽  
Clinical Appearance ◽  
Pathological Appearance ◽  
Stratified Squamous Epithelium ◽  
Well Differentiated ◽  
Squamous Cell Papilloma

ABSTRACTVerrucous carcinoma (VC) of oral cavity is a rare variant of well-differentiated squamous cell carcinoma and squamous papilloma is a benign proliferation of the stratified squamous epithelium, which results in a papillary or verrucous exophytic mass. There is a certain clinical similarity between squamous cell papilloma and VC. We presented a report of two cases which are VC and squamous cell papilloma that are showed the same clinical appearance but different pathological appearance, with a review of the literature.

scholarly journals Morphology of the tongue of the emu (Dromaius novaehollandiae). II. Histological features

2009 ◽  
Vol 76 (4) ◽  
Author(s):  
M.R. Crole ◽  
J.T. Soley
Keyword(s):  
Lymphoid Tissue ◽  
Squamous Epithelium ◽  
Taste Buds ◽  
Taste Bud ◽  
Histological Structure ◽  
Histological Features ◽  
Caudal Extremity ◽  
Dromaius Novaehollandiae ◽  
Stratified Squamous Epithelium ◽  
Nodular Tissue

Although a number of brief, fragmented descriptions have been provided on the gross morphology of the ratite tongue, very few studies have documented the histological structure of this organ. This paper presents the first definitive histological description of the emu tongue and reviews, consolidates and compares the scattered information on the histology of the ratite tongue available in the literature. Five tongues were removed from heads obtained from birds at slaughter and fixed in 10 % neutral buffered formalin. Appropriate longitudinal and transverse segments were removed, routinely processed for light microscopy, and sections examined after staining with H & E and PAS. The entire tongue (body and root) is invested by a non-keratinized stratified squamous epithelium. The supporting connective tissue of the tongue dorsum displays only large, simple branched tubular mucussecreting glands, whereas the caudal tongue body ventrum and tongue root, in addition to these glands, also exhibits small, simple tubular mucus-secreting glands. Herbst corpuscles are associated with the large, simple branched glands. Lymphoid tissue is restricted to the tongue ventrum and is particularly obvious at the junction of the ventral tongue body and frenulum where a large aggregation of diffuse lymphoid tissue, with nodular tissue proximally, was consistently observed. A structure resembling a taste bud was located in the epithelium on the caudal extremity of the tongue root of one bird. This is the first reported observation of taste buds in ratites. Forming the core of the tongue body is the cartilaginous paraglossum lying dorsal to the partially ossified rostral projection of the basihyale. The histological features of the emu tongue are generally similar to those described for the greater rhea and ostrich, except that taste buds were not identified in these species. The results would suggest that the emu tongue functions as a sensory organ, both for taste and touch (by virtue of taste receptors and Herbst corpuscles, respectively), as well as fulfilling an immunological function.

Junction of the Epithelium and Lamina Propria of the Bovine Rumen Mucosa

1967 ◽  
Vol 25 ◽  
pp. 68-69
Author(s):  
Al W. Stinson
Keyword(s):  
Osmium Tetroxide ◽  
Squamous Epithelium ◽  
Short Chain Fatty Acids ◽  
Chloride Ions ◽  
Fermentation Products ◽  
Ruminant Species ◽  
Protective Shield ◽  
Stratified Squamous Epithelium ◽  
Lead Hydroxide ◽  
Acetic Acids

The stratified squamous epithelium which lines the ruminal compartment of the bovine stomach performs at least three important functions. (1) The upper keratinized layer forms a protective shield against the rough, fibrous, constantly moving ingesta. (2) It is an organ of absorption since a number of substances are absorbed directly through the epithelium. These include short chain fatty acids, potassium, sodium and chloride ions, water, and many others. (3) The cells of the deeper layers metabolize butyric acid and to a lesser extent propionic and acetic acids which are the fermentation products of rumen digestion. Because of the functional characteristics, this epithelium is important in the digestive process of ruminant species which convert large quantities of rough, fibrous feed into energy.Tissue used in this study was obtained by biopsy through a rumen fistula from clinically healthy, yearling holstein steers. The animals had been fed a typical diet of hay and grain and the ruminal papillae were fully developed. The tissue was immediately immersed in 1% osmium tetroxide buffered to a pH of 7.4 and fixed for 2 hrs. The tissue blocks were embedded in Vestapol-W, sectioned with a Porter-Blum microtome with glass knives and stained with lead hydroxide. The sections were studied with an RCA EMU 3F electron microscope.

scholarly journals Immortalization and Characterization of Rat Lingual Keratinocytes in a High-Calcium and Feeder-Free Culture System Using ROCK Inhibitor Y-27632

2021 ◽  
Vol 22 (13) ◽  
pp. 6782
Author(s):  
Zixing Chen ◽  
Wenmeng He ◽  
Thomas Chun Ning Leung ◽  
Hau Yin Chung
Keyword(s):  
Calcium Imaging ◽  
Cultured Cells ◽  
Rho Kinase ◽  
Culture Method ◽  
Taste Bud ◽  
Label Free ◽  
Sprague Dawley ◽  
Rock Inhibitor ◽  
High Calcium

Cultured keratinocytes are desirable models for biological and medical studies. However, primary keratinocytes are difficult to maintain, and there has been little research on lingual keratinocyte culture. Here, we investigated the effect of Y-27632, a Rho kinase (ROCK) inhibitor, on the immortalization and characterization of cultured rat lingual keratinocyte (RLKs). Three Y-27632–supplemented media were screened for the cultivation of RLKs isolated from Sprague–Dawley rats. Phalloidin staining and TUNEL assay were applied to visualize cytoskeleton dynamics and cell apoptosis following Y-27632 removal. Label-free proteomics, RT-PCR, calcium imaging, and cytogenetic studies were conducted to characterize the cultured cells. Results showed that RLKs could be conditionally immortalized in a high-calcium medium in the absence of feeder cells, although they did not exhibit normal karyotypes. The removal of Y-27632 from the culture medium led to reversible cytoskeletal reorganization and nuclear enlargement without triggering apoptosis, and a total of 239 differentially expressed proteins were identified by proteomic analysis. Notably, RLKs derived from the non-taste epithelium expressed some molecular markers characteristic of taste bud cells, yet calcium imaging revealed that they rarely responded to tastants. Collectively, we established a high-calcium and feeder-free culture method for the long-term maintenance of RLKs. Our results shed some new light on the immortalization and differentiation of lingual keratinocytes.

Note on the Transformation of Ciliated into Stratified Squamous Epithelium as a result of the Application of Friction

1890 ◽  
Vol 16 ◽  
pp. 119-120 ◽  
Author(s):  
John Berry Haycraft ◽  
E. W. Carlier
Keyword(s):  
Mucous Membrane ◽  
Squamous Epithelium ◽  
The Other ◽  
Ciliated Epithelium ◽  
Deep Groove ◽  
Stratified Squamous Epithelium ◽  
Trachealis Muscle

(Abstract.)In man, in the rabbit, and some other animals the trachea is built up of a series of cartilaginous rings incomplete behind; the rings being completed in this position by the trachealis muscle.The mucous membrane forms a smooth cylindrical lining for the whole tube, and is covered by a ciliated epithelium.In the cat and dog the cartilage rings completely encircle the trachea, and overlap posteriorly, and the trachealis muscle, which is well developed, is placed outside the cartilage, and has a powerful action in varying the diameter of the tube.When this muscle contracts the overlapping ends of the cartilage ride one upon another, and the projecting ends form a vertical ridge down the inside of the trachea, which can be readily seen on slitting the organ open. This ridge is separated by a deep groove from the other end of the plate.

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