scholarly journals The Arabidopsis RboHB Encoded by At1g09090 Is Important for Resistance against Nematodes

2020 ◽  
Vol 21 (15) ◽  
pp. 5556 ◽  
Author(s):  
Abdalmenem I. M. Hawamda ◽  
Adil Zahoor ◽  
Amjad Abbas ◽  
Muhammad Amjad Ali ◽  
Holger Bohlmann
Keyword(s):  
Reactive Oxygen Species ◽  
Respiratory Burst ◽  
Reactive Oxygen ◽  
Heterodera Schachtii ◽  
Oxygen Species ◽  
Cyst Nematodes ◽  
Feeding Site ◽  
Plant Life ◽  
Respiratory Burst Oxidase ◽  
Respiratory Burst Oxidase Homologue

Reactive oxygen species are a byproduct of aerobic metabolic processes but are also produced by plants in defense against pathogens. In addition, they can function as signaling molecules that control various aspects of plant life, ranging from developmental processes to responses to abiotic and biotic stimuli. In plants, reactive oxygen species can be produced by respiratory burst oxidase homologues. Arabidopsis contains 10 genes for respiratory burst oxidase homologues that are involved in different aspects of plant life. Plant pathogenic cyst nematodes such as Heterodera schachtii induce a syncytium in the roots of host plants that becomes a feeding site which supplies nutrients throughout the life of the nematode. In line with this function, the transcriptome of the syncytium shows drastic changes. One of the genes that is most strongly downregulated in syncytia codes for respiratory burst oxidase homologue B. This gene is root-specific and we confirm here the downregulation in nematode feeding sites with a promoter::GUS (β-glucuronidase) line. Overexpression of this gene resulted in enhanced resistance against nematodes but also against leaf-infecting pathogens. Thus, respiratory burst oxidase homologue B has a role in resistance. The function of this gene is in contrast to respiratory burst oxidase homologues D and F, which have been found to be needed for full susceptibility of Arabidopsis to H. schachtii. However, our bioinformatic analysis did not find differences between these proteins that could account for the opposed function in the interaction with nematodes.

Arabidopsis thaliana constitutively active ROP11 interacts with the NADPH oxidase respiratory burst oxidase homologue F to regulate reactive oxygen species production in root hairs

2016 ◽  
Vol 43 (3) ◽  
pp. 221 ◽  
Author(s):  
Min Yan ◽  
Wen Jing ◽  
Ni Xu ◽  
Like Shen ◽  
Qun Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Arabidopsis Thaliana ◽  
Respiratory Burst ◽  
Root Hairs ◽  
Reactive Oxygen ◽  
Ros Production ◽  
Oxygen Species ◽  
Wild Type ◽  
Respiratory Burst Oxidase ◽  
Constitutively Active

Reactive oxygen species (ROS) play a key signalling role in cells. Plant NADPH oxidases, also known as respiratory burst oxidase homologues (Rbohs), are well characterised ROS-generating systems. In this study, we found that the constitutively active small guanosine triphosphatase (GTPase) ROP11 (CA-ROP11) interacted with RbohF by using a yeast two-hybrid analysis, a pull-down assay and an in vivo bimolecular fluorescence complementation assay. The mutation of amino acid L336 or L337 in RbohF abolished its interaction with CA-ROP11. Coexpression of CA-ROP11 and wild-type RbohF in Nicotiana benthamiana Domin enhanced ROS production compared with coexpression of CA-ROP11 and mutant RbohF or of dominant negative ROP11 and wild-type RbohF. Moreover, CA-ROP11 overexpression resulted in ROS accumulation and a swollen root hair phenotype in Arabidopsis thaliana (L.) Heynh. The deletion of RbohF partially reduced the increase in ROS in Arabidopsis plants overexpressing CA-ROP11. These results suggest that Arabidopsis ROP11 modulates ROS production by interacting with RbohF in root hairs.

Effects of raspberry fruit extracts and ellagic acid on respiratory burst in murine macrophages

2014 ◽  
Vol 5 (6) ◽  
pp. 1167-1174 ◽  
Author(s):  
Lina Raudone ◽  
Ramune Bobinaite ◽  
Valdimaras Janulis ◽  
Pranas Viskelis ◽  
Sonata Trumbeckaite
Keyword(s):  
Reactive Oxygen Species ◽  
Respiratory Burst ◽  
Ellagic Acid ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Murine Macrophages ◽  
Fruit Extracts ◽  
Species Production

The main finding of our study is that raspberry extracts and ellagic acid inhibit reactive oxygen species production in PMA stimulated macrophages.

scholarly journals Alteration of Neutrophil Reactive Oxygen Species Production by Extracts of Devil’s Claw (Harpagophytum)

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Mbaki Muzila ◽  
Kimmo Rumpunen ◽  
Helen Wright ◽  
Helen Roberts ◽  
Melissa Grant ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Respiratory Burst ◽  
Dna Analysis ◽  
Reactive Oxygen ◽  
Human Neutrophils ◽  
Putative Hybrid ◽  
Oxygen Species ◽  
New Variety ◽  
Devil’S Claw ◽  
Anti Inflammatory

Harpagophytum, Devil’s Claw, is a genus of tuberiferous xerophytic plants native to southern Africa. Some of the taxa are appreciated for their medicinal effects and have been traditionally used to relieve symptoms of inflammation. The objectives of this pilot study were to investigate the antioxidant capacity and the content of total phenols, verbascoside, isoverbascoside, and selected iridoids, as well as to investigate the capacity of variousHarpagophytumtaxa in suppressing respiratory burst in terms of reactive oxygen species produced by human neutrophils challenged with phorbol myristate acetate (PMA), opsonisedStaphylococcus aureus,andFusobacterium nucleatum.Harpagophytumplants were classified into different taxa according to morphology, and DNA analysis was used to confirm the classification. A putative new variety ofH. procumbensshowed the highest degree of antioxidative capacity. Using PMA, threeHarpagophytumtaxa showed anti-inflammatory effects with regard to the PBS control. A putative hybrid betweenH. procumbensandH. zeyheriin contrast showed proinflammatory effect on the response of neutrophils toF. nucleatumin comparison with treatment with vehicle control.Harpagophytumtaxa were biochemically very variable and the response in suppressing respiratory burst differed. Further studies with larger number of subjects are needed to corroborate anti-inflammatory effects of different taxa ofHarpagophytum.

Surfactant protein A modulates release of reactive oxygen species from alveolar macrophages

1994 ◽  
Vol 267 (6) ◽  
pp. L660-L666 ◽  
Author(s):  
S. Weissbach ◽  
A. Neuendank ◽  
M. Pettersson ◽  
T. Schaberg ◽  
U. Pison
Keyword(s):  
Reactive Oxygen Species ◽  
Respiratory Burst ◽  
Protein A ◽  
Reactive Oxygen ◽  
Surfactant Protein ◽  
Oxygen Radical ◽  
Surfactant Protein A ◽  
Oxygen Species ◽  
Coated Surfaces

The production and release of reactive oxygen species (the respiratory burst) is a common metabolic pathway linked to several macrophage-related reactions. The most abundant surfactant protein A (SP-A) binds to alveolar macrophages (AM) through a specific surface receptor with high affinity. Because such binding might initiate or modulate the respiratory burst, we wanted to know whether and how SP-A affects the oxygen radical release from AM. To answer these questions, we measured the release of reactive oxygen species from rat AM under various in vitro conditions using enhanced chemiluminescence systems. We prepared SP-A from pulmonary surfactant isolated either from silica-treated rats or adult dogs. Resident AM were harvested from pathogen-free Wistar rats by lung lavage. Adhered and nonadhered AM were assessed on protein-free or protein-coated surfaces of 96-well microtiter plates. On protein-free surfaces, the sole addition of SP-A failed to induce measurable oxygen radical release from 2 x 10(5) adhered or nonadhered AM, while zymosan opsonized with SP-A induced a marked increase over control. On protein-coated surfaces, AM respond differently depending on the coated protein: on SP-A-coated surfaces, a dose-dependent enhancement of oxygen radical release with a mean effective concentration of approximately 1.15 micrograms/ml was found. No such enhancement was seen on plates coated with similar amounts of either human fibronectin or collagen, and the enhancement with serum albumin was not dose related. Our data demonstrate that SP-A only enhances oxygen radical release from AM if SP-A is fixed to zymosan or the surface of the reaction vial in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Plasmodesmata-localized proteins and reactive oxygen species orchestrate light-induced rapid systemic signaling in Arabidopsis

2020 ◽  
Author(s):  
Yosef Fichman ◽  
Ronald J. Myers ◽  
DeAna G. Grant ◽  
Ron Mittler
Keyword(s):  
Reactive Oxygen Species ◽  
Light Stress ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Cell Transport ◽  
Systemic Signaling ◽  
Entire Plant ◽  
Systemic Signal ◽  
Respiratory Burst Oxidase ◽  
Systemic Signals

AbstractSystemic signaling and systemic acquired acclimation (SAA) are key to the survival of plants during episodes of abiotic stress. These processes depend on a continuous chain of cell-to-cell signaling events that extends from the initial tissue that senses the stress (local tissue) to the entire plant (systemic tissues). Among the different systemic signaling molecules and processes thought to be involved in this cell-to-cell signaling mechanism are reactive oxygen species (ROS), calcium, electric and hydraulic signals. How these different signals and processes are interlinked, and how they transmit the systemic signal all the way from the local tissue to the entire plant, remain however largely unknown. Here, studying the systemic response of Arabidopsis thaliana to a local treatment of excess light stress, we report that respiratory burst oxidase homolog D (RBOHD)-generated ROS enhance cell-to-cell transport and plasmodesmata (PD) pore size in a process that depends on the function of PD-localized proteins (PDLPs) 1 and 5, promoting the cell-to-cell transport of systemic signals during responses to light stress. We further identify aquaporins, and several different calcium-permeable channels, belonging to the glutamate receptor-like, mechanosensitive small conductance-like, and cyclic nucleotide-gated families, as involved in this process, but determine that their function is primarily required for the maintenance of the signal in each cell along the path of the systemic signal, as well as for the establishment of acclimation at the local and systemic tissues. PD and RBOHD-generated ROS orchestrate therefore light stress-induced rapid cell-to-cell spread of systemic signals in Arabidopsis.One-sentence summaryRespiratory burst oxidase homolog D-generated reactive oxygen species enhance cell-to-cell transport and plasmodesmata (PD) pore size in a process that depends on the function of the PD-localized proteins (PDLPs) 1 and 5, promoting the cell-to-cell transport of rapid systemic signals during the response of Arabidopsis to excess light stress.

scholarly journals Production of reactive oxygen species by PuRBOHF is critical for stone cell development in pear fruit

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaoqian Wang ◽  
Siqi Liu ◽  
Huili Sun ◽  
Chunyan Liu ◽  
Xinyue Li ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Formation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Pear Fruit ◽  
Specific Expression ◽  
Stone Cell ◽  
Transient Overexpression ◽  
Microscopy Data ◽  
Respiratory Burst Oxidase

AbstractThe production of reactive oxygen species (ROS) by NADPH oxidase, which is also referred to as respiratory burst oxidase homolog (RBOH), affects several processes in plants. However, the role of RBOHs in cell wall lignification is not well understood. In this study, we show that PuRBOHF, an RBOH isoform, plays an important role in secondary wall formation in pear stone cells. ROS were closely associated with lignin deposition and stone cell formation according to microscopy data. In addition, according to the results of an in situ hybridization analysis, the stage-specific expression of PuRBOHF was higher in stone cells than in cells of other flesh tissues. Inhibitors of RBOH activity suppressed ROS accumulation and stone cell lignification in pear fruit. Moreover, transient overexpression of PuRBOHF caused significant changes in the amount of ROS and lignin that accumulated in pear fruit and flesh calli. We further showed that PuMYB169 regulates PuRBOHF expression, while PuRBOHF-derived ROS induces the transcription of PuPOD2 and PuLAC2. The findings of this study indicate that PuRBOHF-mediated ROS production, which is regulated by a lignin-related transcriptional network, is essential for monolignol polymerization and stone cell formation in pear fruit.

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