scholarly journals Environmental DNA and RNA as Records of Human Exposome, Including Biotic/Abiotic Exposures and Its Implications in the Assessment of the Role of Environment in Chronic Diseases

2020 ◽  
Vol 21 (14) ◽  
pp. 4879 ◽  
Author(s):  
Indu Shekhar Thakur ◽  
Deodutta Roy
Keyword(s):  
Chronic Diseases ◽  
Gene Networks ◽  
Environmental Dna ◽  
Environmental Stressors ◽  
Great Promise ◽  
Multiple Exposures ◽  
Dna And Rna ◽  
Dynamic Stochastic ◽  
Environmental Causes ◽  
Development And Validation

Most of environment-related diseases often result from multiple exposures of abiotic and/or biotic stressors across various life stages. The application of environmental DNA/RNA (eDNA/eRNA) to advance ecological understanding has been very successfully used. However, the eminent extension of eDNA/eRNA-based approaches to estimate human exposure to biotic and/or abiotic environmental stressors to understand the environmental causes of chronic diseases has yet to start. Here, we introduce the potential of eDNA/eRNA for bio-monitoring of human exposome and health effects in the real environmental or occupational settings. This review is the first of its kind to discuss how eDNA/eRNA-based approaches can be applied for assessing the human exposome. eDNA-based exposome assessment is expected to rely on our ability to capture the genome- and epigenome-wide signatures left behind by individuals in the indoor and outdoor physical spaces through shedding, excreting, etc. Records of eDNA/eRNA exposome may reflect the early appearance, persistence, and presence of biotic and/or abiotic-exposure-mediated modifications in these nucleic acid molecules. Functional genome- and epigenome-wide mapping of eDNA offer great promise to help elucidate the human exposome. Assessment of longitudinal exposure to physical, biological, and chemical agents present in the environment through eDNA/eRNA may enable the building of an integrative causal dynamic stochastic model to estimate environmental causes of human health deficits. This model is expected to incorporate key biological pathways and gene networks linking individuals, their geographic locations, and random multi-hits of environmental factors. Development and validation of monitoring of eDNA/eRNA exposome should seriously be considered to introduce into safety and risk assessment and as surrogates of chronic exposure to environmental stressors. Here we highlight that eDNA/eRNA reflecting longitudinal exposure of both biotic and abiotic environmental stressors may serve as records of human exposome and discuss its application as molecular tools for understanding the toxicogenomics basis of environment-related health deficits.

scholarly journals Development and validation of an environmental DNA protocol to detect an invasive Caribbean freshwater fish, the guppy ( Poecilia reticulata )

2021 ◽  
Author(s):  
Sierra N. Smith ◽  
Ingo Schlupp ◽  
Edward D. Higgins ◽  
Jessa L. Watters ◽  
Kerri‐Ann Bennett ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
Poecilia Reticulata ◽  
Environmental Dna ◽  
Development And Validation

scholarly journals Linking Environmental DNA and RNA for Improved Detection of the Marine Invasive Fanworm Sabella spallanzanii

2019 ◽  
Vol 6 ◽  
Author(s):  
Ulla von Ammon ◽  
Susanna A. Wood ◽  
Olivier Laroche ◽  
Anastasija Zaiko ◽  
Shane D. Lavery ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Dna And Rna

scholarly journals Development and validation of an environmental DNA test for the endangered Gouldian finch

2019 ◽  
Vol 40 ◽  
pp. 171-182 ◽  
Author(s):  
K Day ◽  
H Campbell ◽  
A Fisher ◽  
K Gibb ◽  
B Hill ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Dna Test ◽  
Gouldian Finch ◽  
Development And Validation

Clearer than mud? Using environmental DNA to track historical shifts in lake communities.

2021 ◽  
Author(s):  
John Pearman ◽  
Laura Biessy ◽  
Georgia Thomson-Laing ◽  
Lizette Reyes ◽  
Claire Shepherd ◽  
...  
Keyword(s):  
New Zealand ◽  
Microbial Communities ◽  
Environmental History ◽  
Environmental Changes ◽  
Environmental Dna ◽  
Taxonomic Composition ◽  
Molecular Methods ◽  
Rrna Gene ◽  
Metabolic Potential ◽  
Dna And Rna

<p>A continuous record of environmental history is stored in lake sediments providing an avenue to explore current and historical lake communities. Traditionally paleolimnological methods have focussed on macroscopic indicators (e.g. pollen, chronomids, diatoms) to investigate environmental changes but the application of environmental DNA techniques has enabled the investigation of microbial communities and other soft bodied organisms through time. The ‘Our lakes’ health; past, present, future (Lakes380)’ project aims to combined traditional and molecular methods to explore shifts in biological communities over the last 1,000 years (pre-human arrival in New Zealand). Sediments cores have been collected from a wide diversity of lakes across New Zealand and 16S rRNA gene metabarcoding approaches of both DNA and RNA applied to reveal how microbial community changes across time and especially in response to the arrival of humans and associated changes to the landscape and lake environments. We further investigate the changes in inferred metabolic potential of the microbial communities as the taxonomic composition of the lake differs over time. Finally, we combine these novel molecular methods with hyperspectral scanning and pollen data to increase the knowledge of changes in lake communities and identifying the timing of changes in lake health. The combination of methodologies provides a greater understanding of the environmental history of lake systems and will help to inform management decisions relating to the restoration and protection of lake health.</p>

Sign in / Sign up

Export Citation Format

Share Document