scholarly journals Development and validation of an environmental DNA protocol to detect an invasive Caribbean freshwater fish, the guppy ( Poecilia reticulata )

2021 ◽  
Author(s):  
Sierra N. Smith ◽  
Ingo Schlupp ◽  
Edward D. Higgins ◽  
Jessa L. Watters ◽  
Kerri‐Ann Bennett ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
Poecilia Reticulata ◽  
Environmental Dna ◽  
Development And Validation

scholarly journals The alien freshwater fish of Mount Galunggung, West Java, Indonesia

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Haryono Haryono ◽  
Gema Wahyudewantoro
Keyword(s):  
Freshwater Fish ◽  
Poecilia Reticulata ◽  
Active Volcano ◽  
Ornamental Fish ◽  
Survey Method ◽  
Fish Diversity ◽  
Local Distribution ◽  
Introduced Fish ◽  
West Java ◽  
Alien Fish

Abstract. Haryono, Wahyudewantoro G. 2020. The alien freshwater fish of Mount Galunggung, West Java, Indonesia. Biodiversitas 21: 1401-1408. Mt. Galunggung, located in West Java, Indonesia, is an active volcano renowned for its deadly eruption in 1982. This area has many waters bodies, but the fish communities have not been recorded, especially the alien or introduced fish species. Hence, the objective of the present study was to determine the alien fish diversity, abundance, local distribution and utilization in Galunggung vicinity. The study was done in March 2013 using survey method covering 10 stations. Fish sampling was based on catch per unit of effort (CPUE) using mainly electrofishing, gillnet and cast net. A total of 24 species were recorded during the survey, in which 13 were listed as introduced species. Poecilia reticulata was the dominant species with 7.4 ind./St and followed by Xiphophorus helleri with 5.3 ind./St. Both species were also widely distributed on the sites with 70% local distribution. The alien species in this area are mostly used for ornamental fish.

Monitoring freshwater fish communities in large rivers using environmental DNA metabarcoding and a long‐term electrofishing survey

2020 ◽  
Vol 97 (2) ◽  
pp. 444-452 ◽  
Author(s):  
Aurélie Goutte ◽  
Noëlie Molbert ◽  
Sabrina Guérin ◽  
Robin Richoux ◽  
Vincent Rocher
Keyword(s):  
Freshwater Fish ◽  
Fish Communities ◽  
Environmental Dna ◽  
Large Rivers ◽  
Dna Metabarcoding

scholarly journals Development and validation of an environmental DNA test for the endangered Gouldian finch

2019 ◽  
Vol 40 ◽  
pp. 171-182 ◽  
Author(s):  
K Day ◽  
H Campbell ◽  
A Fisher ◽  
K Gibb ◽  
B Hill ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Dna Test ◽  
Gouldian Finch ◽  
Development And Validation

scholarly journals Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0130965 ◽  
Author(s):  
Christina M. Davy ◽  
Anne G. Kidd ◽  
Chris C. Wilson
Keyword(s):  
Environmental Dna ◽  
Freshwater Turtles ◽  
Development And Validation

scholarly journals Alpine freshwater fish biodiversity assessment: an intercalibration test for metabarcoding method set up

2021 ◽  
Vol 4 ◽  
Author(s):  
Giulia Riccioni ◽  
Isabelle Domaizon ◽  
Andrea Gandolfi ◽  
Massimo Pindo ◽  
Marine Vautier ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
Population Decline ◽  
Environmental Dna ◽  
Well Being ◽  
Taxonomic Resolution ◽  
Anthropogenic Pressure ◽  
Biodiversity Assessment ◽  
Good Efficiency ◽  
Essential Information ◽  
Fish Biodiversity

Environmental DNA (eDNA) based methods (Fig. 1) are proving to be a promising tool for freshwater fish biodiversity assessment in Europe within the Water Framework Directive (WFD, 2000/60/EC) especially for large rivers and lakes where current fish monitoring techniques have known shortcomings. Freshwater fish are actively involved in aquatic ecosystems functioning and diversity, contributing to the health, well-being and economy in every geographic realm. Unfortunately, many freshwater fish are experiencing critical population decline with risk of local or global extinction because of intense anthropogenic pressure. Within the EU project Eco-AlpsWater, advanced high throughput sequencing (HTS) techniques are used to improve the traditional WFD monitoring approaches by using environmental DNA (eDNA) collected in Alpine waterbodies. To evaluate the performance of the metabarcoding approach specifically designed to measure freshwater fish biodiversity in Alpine lakes and rivers, an intercalibration test was performed. This exercise forecasted the use of mock samples containing either tissue-extracted DNA of different target species or water collected from aquaculture tanks to mimic real environmental water sampling and processing. Moreover, three water samples collected in Lake Bourget (France) were used to compare the efficiency of taxonomic assignments in natural and mock community samples. Our results highlighted a good efficiency of the molecular laboratory protocols for HTS and a good amplification success of the selected primers, providing essential information concerning the taxonomic resolution of the 12S mitochondrial marker. As further confirmation, different concentration of species DNA in the mock samples were well represented by the relative read abundance. This preliminary test confirmed the applicability of eDNA metabarcoding analyses for the biomonitoring of freshwater fish inhabiting Alpine and perialpine lakes and rivers.

scholarly journals Evaluating Importation of Aquatic Ornamental Species for Biosecurity Purposes

2022 ◽  
Vol 9 ◽  
Author(s):  
Javier Atalah ◽  
Ian C. Davidson ◽  
Maike Thoene ◽  
Eugene Georgiades ◽  
Kate S. Hutson
Keyword(s):  
New Zealand ◽  
Freshwater Fish ◽  
Poecilia Reticulata ◽  
Future Climate ◽  
Indigenous Species ◽  
Climate Scenarios ◽  
Industry Growth ◽  
Climate Conditions ◽  
Regulatory Decision ◽  
Ornamental Species

The aquatic ornamental species (AOS) trade is a significant pathway for the introduction and establishment of non-indigenous species into aquatic environments. The likelihood of such occurrences is expected to increase worldwide as industry growth continues and warmer conditions emerge under future climate scenarios. This study used recent (2015 – 2019) New Zealand importation data to determine the composition, diversity, abundance, and arrival frequency of AOS. Our analysis revealed that ca. 300,000 aquatic ornamental individuals are imported annually to New Zealand, with freshwater fish comprising 98% of import quantities. Despite the relatively small market size, the estimated AOS diversity of 865 taxa (89 and 9.5% identified to species and genus level, respectively) is comparable to larger markets with ∼60% of taxa being of marine origin. Species (n = 20) for further investigation were prioritized based on quantity and frequency of import. These prioritized AOS were exclusively tropical and subtropical freshwater fish and align with the most frequently imported AOS globally, including the top three: neon tetra (Paracheirodon innesi), guppy (Poecilia reticulata), and tiger barb (Puntigrus tetrazona). Species distribution modeling of the 20 prioritized AOS predicted that 13 species are suitable for New Zealand’s current climate conditions, most notably sucker-belly loach (Pseudogastromyzon myersi), white cloud mountain minnow (Tanichthys albonubes), and golden otocinclus (Macrotocinclus affinis). Potential changes in habitat suitability were predicted under future climate scenarios, with largest increases (29%) for Po. reticulata. The described approach provides an adaptable framework to assess establishment likelihood of imported AOS to inform regulatory decision making.

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