scholarly journals Ectopic Expression of OsPYL/RCAR7, an ABA Receptor Having Low Signaling Activity, Improves Drought Tolerance without Growth Defects in Rice

2020 ◽  
Vol 21 (11) ◽  
pp. 4163
Author(s):  
Nikita Bhatnagar ◽  
Rigyeong Kim ◽  
Seungsu Han ◽  
Jaeeun Song ◽  
Gang Seob Lee ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Stress Tolerance ◽  
Transgenic Rice ◽  
Expression System ◽  
Total Yield ◽  
Ectopic Expression ◽  
Aba Signaling ◽  
Aba Receptor ◽  
Signaling Activity ◽  
Aba Receptors

Overexpression of abscisic acid (ABA) receptors has been reported to enhance drought tolerance, but also to cause stunted growth and decreased crop yield. Here, we constructed transgenic rice for all monomeric ABA receptors and observed that only transgenic rice over-expressing OsPYL/RCAR7 showed similar phenotype with wild type, without total yield loss when grown under normal growth condition in a paddy field. Even though transgenic rice over-expressing OsPYL/RCAR7 showed neither an ABA-sensitivity nor an osmotic stress tolerance in plate assay, it showed drought tolerance. We investigated the ABA-dependent interaction with OsPP2CAs and ABA signaling induction by OsPYL/RCAR7. In yeast two hybrid assay, OsPYL/RCAR7 required critically higher ABA concentrations to interact with OsPP2CAs than other ABA receptors, and co-immunoprecipitation assay showed strong interaction under ABA treatment. When ABA-responsive signaling activity was monitored using a transient expression system in rice protoplasts, OsPYL/RCAR7 had the lowest ABA-responsive signaling activity as compared with other ABA receptors. OsPYL/RCAR7 also showed weak suppression of phosphatase activity as compared with other ABA receptors in vitro. Transcriptome analysis of transgenic rice over-expressing OsPYL/RCAR7 suggested that only a few genes were induced similar to control under without exogenous ABA, but a large number of genes was induced under ABA treatment compared with control. We conclude that OsPYL/RCAR7 is a novel functional ABA receptor that has low ABA signaling activity and exhibits high ABA dependence. These results lay the foundation for a new strategy to improve drought stress tolerance without compromising crop growth.

scholarly journals Jacalin domain-containing protein OsSalT interacts with OsDREB2A and OsNAC1 to impart drought stress tolerance in planta

2020 ◽  
Author(s):  
Salman Sahid ◽  
Chandan Roy ◽  
Dibyendu Shee ◽  
Riddhi Datta ◽  
Soumitra Paul
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Stress Tolerance ◽  
Ectopic Expression ◽  
Climatic Conditions ◽  
Stress Factors ◽  
Bimolecular Fluorescence Complementation ◽  
In Planta ◽  
Drought Stress Tolerance ◽  
Transgenic Lines

AbstractWith the changing climatic conditions, drought has become one of the most threatening abiotic stress factors that adversely affect rice cultivation and productivity. Although the involvement of the jacalin domain-containing protein, OsSalT, has been reported in drought and salinity tolerance, its functional mechanism still remains largely unexplored. In this study, expression of the OsSalT gene was found to be positively correlated with the drought tolerance potential with its higher transcript abundance in the tolerant indica rice cultivar, Vandana and lower abundance in the susceptible cultivar, MTU1010. Moreover, the ectopic expression of OsSalT in tobacco imparted drought stress tolerance in the transgenic lines. The transgenic lines exhibited significantly improved growth and higher osmolyte accumulation over the wild-type (WT) plants together with the induction in the OsSalT expression under drought stress. Fascinatingly, the yeast two-hybrid and bimolecular fluorescence complementation (BiFC) analyses confirmed the interaction of OsSalT protein with two interesting transcription factors (TFs), OsNAC1 and OsDREB2A. In silico analysis further revealed that the OsSalT protein interacted with the regulatory domain of OsDREB2A and the C-terminal domain of OsNAC1 leading to their activation and induction of their downstream drought-responsive genes. Together, this study unravels a novel model for OsSalT-mediated regulation of drought tolerance in plants.

scholarly journals Systematic analyses of the roles of Solanum Lycopersicum ABA receptors in environmental stress and development

2016 ◽  
Author(s):  
Assaf Mosquna ◽  
Sean Cutler
Keyword(s):  
Water Balance ◽  
Stress Tolerance ◽  
Abiotic Stresses ◽  
Large Scale ◽  
Abiotic Stress Tolerance ◽  
Physiological Role ◽  
Initial Study ◽  
Aba Signaling ◽  
Negative Effects ◽  
Aba Receptors

Drought and other abiotic stresses have major negative effects on agricultural productivity. The plant hormone abscisic acid (ABA) regulates many responses to environmental stresses and can be used to improve crop performance under stress. ABA levels rise in response to diverse abiotic stresses to coordinate physiological and metabolic responses that help plants survive stressful environments. In all land plants, ABA receptors are responsible for initiating a signaling cascade that leads to stomata closure, growth arrest and large-scale changes in transcript levels required for stress tolerance. We wanted to test the meaning of root derived ABA signaling in drying soil on water balance. To this end we generated transgenic tomato lines in which ABA signaling is initiated by a synthetic agonist- mandipropamid. Initial study using a Series of grafting experiments indicate that that root ABA signaling has no effect on the immediate regulation of stomata aperture. Once concluded, these experiments will enable us to systematically dissect the physiological role of root-shoot interaction in maintaining the water balance in plants and provide new tools for targeted improvement of abiotic stress tolerance in crop plants. 

scholarly journals Abscisic Acid Receptors Modulate Metabolite Levels and Phenotype in Arabidopsis Under Normal Growing Conditions

Metabolites ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 249 ◽  
Author(s):  
Xiaoyi Li ◽  
Lintao Wu ◽  
Yao Qiu ◽  
Tao Wang ◽  
Qin Zhou ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Plant Growth ◽  
Aba Signaling ◽  
Primary Metabolite ◽  
Abnormal Growth ◽  
Phenotype Analysis ◽  
Positive Regulators ◽  
Aba Receptor ◽  
Growing Conditions ◽  
Aba Receptors

Abscisic acid (ABA) is a vital phytohormone that accumulates in response to various biotic and abiotic stresses, as well as plant growth. In Arabidopsis thaliana, there are 14 members of the ABA receptor family, which are key positive regulators involved in ABA signaling. Besides reduced drought stress tolerance, the quadruple and sextuple mutants (pyr1pyl1pyl2pyl4 (1124) and pyr1pyl1pyl2pyl4pyl5pyl8 (112458) show abnormal growth phenotypes, such as decreases in yield and height, under non-stress conditions. However, it remains unknown whether ABA receptors mediate ABA signaling to regulate plant growth and development. Here, we showed the primary metabolite profiles of 1124, 112458 and wild-type (WT) plants grown under normal conditions. The metabolic changes were significantly different between ABA receptor mutants and WT. Guanosine, for the biosynthesis of cyclic guanosine 3′,5′-monophosphate (cGMP), is an important second messenger that acts to regulate the level of ABA. In addition, other amino acids were increased in the 112458 mutant, including proline. These results, together with phenotype analysis, indicated that ABA receptors are involved in ABA signaling to modulate metabolism and plant growth under normal conditions.

scholarly journals Development and drought tolerance assay of marker-free transgenic rice with OsAPX2 using biolistic particle-mediated co-transformation

2017 ◽  
Vol 5 (4) ◽  
pp. 271-281 ◽  
Author(s):  
Dan Feng ◽  
Yanwei Wang ◽  
Jinxia Wu ◽  
Tiegang Lu ◽  
Zhiguo Zhang
Keyword(s):  
Drought Tolerance ◽  
Transgenic Rice ◽  
Marker Free

scholarly journals Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane H+-ATPase 2

2021 ◽  
Vol 7 (12) ◽  
pp. eabd4113
Author(s):  
Rui Miao ◽  
Wei Yuan ◽  
Yue Wang ◽  
Irene Garcia-Maquilon ◽  
Xiaolin Dang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Root Growth ◽  
Experimental System ◽  
Aba Signaling ◽  
Wild Type ◽  
Normal Medium ◽  
C Terminus ◽  
Quadruple Mutant ◽  
Aba Insensitive ◽  
Aba Receptors

The hab1-1abi1-2abi2-2pp2ca-1 quadruple mutant (Qabi2-2) seedlings lacking key negative regulators of ABA signaling, namely, clade A protein phosphatases type 2C (PP2Cs), show more apoplastic H+ efflux in roots and display an enhanced root growth under normal medium or water stress medium compared to the wild type. The presence of low ABA concentration (0.1 micromolar), inhibiting PP2C activity via monomeric ABA receptors, enhances root apoplastic H+ efflux and growth of the wild type, resembling the Qabi2-2 phenotype in normal medium. Qabi2-2 seedlings also demonstrate increased hydrotropism compared to the wild type in obliquely-oriented hydrotropic experimental system, and asymmetric H+ efflux in root elongation zone is crucial for root hydrotropism. Moreover, we reveal that Arabidopsis ABA-insensitive 1, a key PP2C in ABA signaling, interacts directly with the C terminus of Arabidopsis plasma membrane H+-dependent adenosine triphosphatase 2 (AHA2) and dephosphorylates its penultimate threonine residue (Thr947), whose dephosphorylation negatively regulates AHA2.

Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter

2008 ◽  
Vol 28 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Xiaolan Wu ◽  
Yoko Shiroto ◽  
Sachie Kishitani ◽  
Yukihiro Ito ◽  
Kinya Toriyama
Keyword(s):  
Drought Tolerance ◽  
Transgenic Rice ◽  
Rice Seedlings
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