scholarly journals Development and Characterization of an Allergic Asthma Rat Model for Interventional Studies

2020 ◽  
Vol 21 (11) ◽  
pp. 3841 ◽  
Author(s):  
Marta Périz ◽  
Francisco J. Pérez-Cano ◽  
Maria J. Rodríguez-Lagunas ◽  
Trinitat Cambras ◽  
Santiago Pastor-Soplin ◽  
...  
Keyword(s):  
Body Temperature ◽  
Allergic Asthma ◽  
Rat Model ◽  
Lavage Fluid ◽  
Brown Norway ◽  
Bordetella Pertussis Toxin ◽  
Objective Model ◽  
Norway Rats ◽  
Anaphylactic Response

Allergic asthma is one of the most common chronic diseases of the airways, however it still remains underdiagnosed and hence undertreated. Therefore, an allergic asthma rat model would be useful to be applied in future therapeutic strategy studies. The aim of the present study was to develop an objective model of allergic asthma in atopic rats that allows the induction and quantification of anaphylactic shock with quantitative variables. Female Brown Norway rats were intraperitoneally sensitized with ovalbumin (OVA), alum and Bordetella pertussis toxin and boosted a week later with OVA in alum. At day 28, all rats received an intranasal challenge with OVA. Anaphylactic response was accurately assessed by changes in motor activity and body temperature. Leukotriene concentration was determined in the bronchoalveolar lavage fluid (BALF), and total and IgE anti-OVA antibodies were quantified in blood and BALF samples. The asthmatic animals’ motility and body temperature were reduced after the shock for at least 20 h. The asthmatic animals developed anti-OVA IgE antibodies both in BALF and in serum. These results show an effective and relatively rapid model of allergic asthma in female Brown Norway rats that allows the quantification of the anaphylactic response.

scholarly journals Attenuating Effect of Peruvian Cocoa Populations on the Acute Asthmatic Response in Brown Norway Rats

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2301
Author(s):  
Marta Périz ◽  
Francisco J. Pérez-Cano ◽  
Trinitat Cambras ◽  
Àngels Franch ◽  
Ivan Best ◽  
...  
Keyword(s):  
Mast Cell ◽  
Allergic Asthma ◽  
Immunoglobulin E ◽  
In Vivo Study ◽  
Brown Norway ◽  
Mast Cell Protease ◽  
Norway Rats ◽  
Anaphylactic Response

Cocoa contains bioactive components, which vary according to genetic and environmental factors. The present study aimed to ascertain the anti-allergic properties of native Peruvian cocoa populations (“Blanco de Piura” or BPC, “Amazonas Peru” or APC, “Criollo de Montaña” or CMC, “Chuncho” or CCC, and an ordinary cocoa or OC). To do so, after an initial in vitro approach, an in vivo study focused on the induction of an anaphylactic response associated with allergic asthma in Brown Norway rats was carried out. Based on their polyphenol content, antioxidant activity and in vitro effects, the APC and CMC were selected to be included in the in vivo study. Cocoa diets were tested in a model of allergic asthma in which anaphylactic response was assessed by changes in body temperature, motor activity and body weight. The concentration of specific immunoglobulin E (IgE), mast cell protease and leukotrienes was also quantified in serum and/or bronchoalveolar lavage fluid. CMC and OC populations exhibited a protective effect on the allergic asthma rat model as evidenced by means of a partial protection against anaphylactic response and, above all, in the synthesis of IgE and the release of mast cell protease.

scholarly journals Motor activity as an unbiased variable to assess anaphylaxis in allergic rats

2015 ◽  
Vol 240 (10) ◽  
pp. 1373-1377 ◽  
Author(s):  
Mar Abril-Gil ◽  
Alba Garcia-Just ◽  
Trinitat Cambras ◽  
Francisco J Pérez-Cano ◽  
Cristina Castellote ◽  
...  
Keyword(s):  
Mast Cell ◽  
Body Temperature ◽  
Motor Activity ◽  
Anaphylactic Shock ◽  
Brown Norway ◽  
Mast Cell Protease ◽  
Specific Immunoglobulin ◽  
Norway Rats ◽  
Anaphylactic Response ◽  
Physiological Systems

The release of mediators by mast cells triggers allergic symptoms involving various physiological systems and, in the most severe cases, the development of anaphylactic shock compromising mainly the nervous and cardiovascular systems. We aimed to establish variables to objectively study the anaphylactic response (AR) after an oral challenge in an allergy model. Brown Norway rats were immunized by intraperitoneal injection of ovalbumin with alum and toxin from Bordetella pertussis. Specific immunoglobulin (Ig) E antibodies were developed in immunized animals. Forty days after immunization, the rats were orally challenged with the allergen, and motor activity, body temperature and serum mast cell protease concentration were determined. The anaphylaxis induced a reduction in body temperature and a decrease in the number of animal movements, which was inversely correlated with serum mast cell protease release. In summary, motor activity is a reliable tool for assessing AR and also an unbiased method for screening new anti-allergic drugs.

Reduction of allergic airway eosinophilia by dietary raffinose in Brown Norway rats

2004 ◽  
Vol 92 (2) ◽  
pp. 247-255 ◽  
Author(s):  
Hiroshi Watanabe ◽  
Kei Sonoyama ◽  
Jun Watanabe ◽  
Natsu Yamaguchi ◽  
Hiroto Kikuchi ◽  
...  
Keyword(s):  
Human Subjects ◽  
Control Diet ◽  
Disease Model ◽  
Intraperitoneal Administration ◽  
Lavage Fluid ◽  
Brown Norway ◽  
Intragastric Administration ◽  
Mrna Levels ◽  
Airway Eosinophilia ◽  
Norway Rats

Oral administration of raffinose, a naturally occurring indigestible oligosaccharide, has reportedly ameliorated atopic dermatitis in human subjects although the mechanism is unknown. The present study investigated the effect of dietary raffinose on allergen-induced airway eosinophilia in ovalbumin-sensitised Brown Norway rats as an atopic disease model. Brown Norway rats were immunised by subcutaneous injection with ovalbumin on day 0 and fed either a control diet or the diet supplemented with raffinose (50 g/kg diet). The rats were exposed to aerosolised ovalbumin on day 20, and broncho-alveolar lavage fluid was obtained on the next day. The number of eosinophils in the fluid was significantly lower in the rats fed the raffinose diet than in those fed the control diet. Dietary raffinose significantly reduced IL-4 and IL-5 mRNA levels in lung tissue and tended to lower ovalbumin-specific Ig E levels. Suppression of eosinophilia by dietary raffinose was still observed in caecectomised and neomycin-administered rats, suggesting little contribution by the colonic bacteria to the effect of raffinose. Intraperitoneal administration of raffinose also suppressed eosinophilia. Significant concentrations of raffinose were detected in portal venous and abdominal arterial plasma after the intragastric administration of raffinose. Overall, the findings suggest that dietary raffinose ameliorates allergic airway eosinophilia at least partly via post-absorptive mechanisms in Brown Norway rats.

Comparison of Arginine Vasopressin, Terlipressin, or Epinephrine to Correct Hypotension in a Model of Anaphylactic Shock in Anesthetized Brown Norway Rats

2006 ◽  
Vol 104 (4) ◽  
pp. 734-741 ◽  
Author(s):  
Pascale Dewachter ◽  
Valérie Jouan-Hureaux ◽  
Isabelle Lartaud ◽  
Gaëlle Bello ◽  
Nicole de Talancé ◽  
...  
Keyword(s):  
Heart Rate ◽  
Mean Arterial Pressure ◽  
Confidence Interval ◽  
Arterial Pressure ◽  
Rat Model ◽  
Arginine Vasopressin ◽  
Anaphylactic Shock ◽  
Brown Norway ◽  
Induced Hypotension ◽  
Norway Rats

Background Arginine vasopressin (AVP) and terlipressin were proposed as alternatives to catecholamines in shock states characterized by decreased plasma AVP concentrations. The endogenous plasma AVP profile in anaphylactic shock is unknown. In an ovalbumin-sensitized anesthetized anaphylactic shock rat model, the authors investigated (1) plasma AVP concentrations and (2) the dose versus mean arterial pressure response for exogenous AVP and terlipressin and compared them with those of epinephrine. Methods In a first series of rats (n = 12), endogenous plasma AVP concentrations were compared with a model of pharmacologically induced hypotension (nicardipine, n = 12). A second series was randomly assigned to three groups (AVP, n = 7; terlipressin, n = 7; epinephrine, n = 7) and dose (AVP: 8 doses, 0.03-100 U/kg; terlipressin: 7 doses, 0.03-30 microg/kg; epinephrine: 7 doses, 0.3-300 microg/kg)-response mean arterial pressure curves were plotted. Data are expressed as mean +/- SD. Results Endogenous plasma AVP concentrations were significantly lower in anaphylactic shock (57 +/- 26 pg/ml) than in the nicardipine group (91 +/- 43 pg/ml; P < 0.05). The ED50 was 10.6 microg/kg (95% confidence interval, 7.1-15.9) for epinephrine and 4.1 U/kg (95% confidence interval, 3.0-5.6) for AVP. Terlipressin did not change mean arterial pressure, regardless of the dose used. Conclusions In a rat model, anaphylactic shock is associated with inadequately low plasma AVP concentrations. For clinically relevant doses, AVP and epinephrine had comparable effects on mean arterial pressure and heart rate values, whereas, unexpectedly, terlipressin was ineffective. These results are consistent with reports in humans experiencing anaphylaxis where AVP injection restored arterial pressure.

scholarly journals Molecular Characterization of Trimellitic Anhydride–induced Respiratory Allergy in Brown Norway Rats

2008 ◽  
Vol 36 (7) ◽  
pp. 985-998 ◽  
Author(s):  
C. Frieke kuper ◽  
Wilbert H. M. Heijne ◽  
Marinus Dansen ◽  
Kitty C. M. Verhoeckx ◽  
Andre Boorsma ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Respiratory Allergy ◽  
Brown Norway ◽  
Trimellitic Anhydride ◽  
Norway Rats
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