scholarly journals Heterogenic Origin of Micro RNAs in Atlantic Salmon (Salmo salar) Seminal Plasma

2020 ◽  
Vol 21 (8) ◽  
pp. 2723
Author(s):  
Teshome Tilahun Bizuayehu ◽  
Igor Babiak
Keyword(s):  
Atlantic Salmon ◽  
Blood Plasma ◽  
Salmo Salar ◽  
Seminal Plasma ◽  
High Throughput Sequencing ◽  
Somatic Cells ◽  
Supporting Cells ◽  
Atlantic Salmon Salmo Salar ◽  
Micro Rnas ◽  
Qpcr Experiment

The origin and contribution of seminal plasma RNAs into the whole semen RNA repertoire are poorly known, frequently being overlooked or neglected. In this study, we used high-throughput sequencing and RT-qPCR to profile microRNA (miRNA) constituents in the whole semen, as well as in fractionated spermatozoa and seminal plasma of Atlantic salmon (Salmo salar). We found 85 differentially accumulated miRNAs between spermatozoa and the seminal plasma. We identified a number of seminal plasma-enriched and spermatozoa-enriched miRNAs. We localized the expression of some miRNAs in juvenile and mature testes. Two abundant miRNAs, miR-92a-3p and miR-202-5p, localized to both spermatogonia and somatic supporting cells in immature testis, and they were also highly abundant in somatic cells in mature testis. miR-15c-5p, miR-30d-5p, miR-93a-5p, and miR-730-5p were detected only in mature testis. miRs 92a-3p, 202-5p, 15c-5p, and 30d-5p were also detected in a juvenile ovary. The RT-qPCR experiment demonstrated lack of correlation in miRNA transcript levels in seminal plasma versus blood plasma. Our results indicate that salmon semen is rich in miRNAs, which are present in both spermatozoa and seminal plasma. Testicular-supporting somatic cells are likely the source of seminal plasma enrichment, whereas blood plasma is unlikely to contribute to the seminal plasma miRNA repertoire.

scholarly journals Heterogenic origin of micro RNAs in Atlantic salmon (Salmo salar) seminal plasma

2019 ◽  
Author(s):  
Teshome Tilahun Bizuayehu ◽  
Igor Babiak
Keyword(s):  
Atlantic Salmon ◽  
Blood Plasma ◽  
Salmo Salar ◽  
Seminal Plasma ◽  
Somatic Cells ◽  
Gonadal Development ◽  
P Value ◽  
Atlantic Salmon Salmo Salar ◽  
Micro Rnas ◽  
Regulatory Functions

Abstract Background The origin and contribution of seminal plasma RNAs into the whole semen RNA repertoire are poorly known, frequently being overlooked or neglected. Virtually nothing is known about seminal plasma RNAs in fish, including small RNAs, which have regulatory functions in gonadal development. Results In this study, we profiled microRNA (miRNA) constituents in the whole semen, as well as in fractionated spermatozoa and seminal plasma of Atlantic salmon (Salmo salar). Among 306 conserved miRNAs, 85 were differentially accumulated (>2 log-fold change and p-value < 0.01) between spermatozoa and the seminal plasma. We identified a number of seminal plasma-enriched and spermatozoa-enriched miRNAs. We localized the expression of some miRNAs in juvenile and mature testes. Two abundant miRNAs, miR-92a-3p and miR-202-5p, localized to both spermatogonia and somatic supporting cells in immature testis, and they were also highly abundant in somatic cells in mature testis. miR-15c-5p, miR-30d-5p, miR-93a-5p, and miR-730-5p were detected only in mature testis. miRs 92a-3p, 202-5p, 15c-5p, and 30d-5p were detected also in a juvenile ovary in locations corresponding to these from the testis. Additional RT-qPCR experiment demonstrated lack of correlation in miRNA transcript levels in seminal plasma versus blood plasma. Conclusions Our results indicate that salmon semen is rich in miRNAs, which are present in both spermatozoa and seminal plasma. The latter ones have partially different profile indicating their heterogenic origin. Testicular supporting somatic cells are likely the source of seminal plasma enrichment, whereas blood plasma unlikely contributes to the seminal plasma miRNA repertoire.

Changes in muscle and blood plasma proteomes of Atlantic salmon (Salmo salar) induced by crowding

Aquaculture ◽  
2010 ◽  
Vol 309 (1-4) ◽  
pp. 272-279 ◽  
Author(s):  
Eva Veiseth-Kent ◽  
Harald Grove ◽  
Ellen M. Færgestad ◽  
Svein O. Fjæra
Keyword(s):  
Atlantic Salmon ◽  
Blood Plasma ◽  
Salmo Salar ◽  
Atlantic Salmon Salmo Salar

Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification

2015 ◽  
Vol 83 (2) ◽  
pp. 238-245.e2 ◽  
Author(s):  
E. Figueroa ◽  
O. Merino ◽  
J. Risopatrón ◽  
V. Isachenko ◽  
R. Sánchez ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Seminal Plasma ◽  
Atlantic Salmon Salmo Salar

Renin, Atrial Natriuretic Peptide and Blood Plasma Ions in Parr and Smolts of Atlantic Salmon Salmo Salar L. and Rainbow Trout Oncorhynchus Mykiss (Walbaum) in Fresh Water and After Short-Term Exposure to Sea Water

1991 ◽  
Vol 157 (1) ◽  
pp. 63-74
Author(s):  
N. F. SMITH ◽  
F. B. EDDY ◽  
A. D. STRUTHERS ◽  
C. TALBOT
Keyword(s):  
Rainbow Trout ◽  
Atlantic Salmon ◽  
Atrial Natriuretic Peptide ◽  
Blood Plasma ◽  
Natriuretic Peptide ◽  
Salmo Salar ◽  
Sea Water ◽  
Atlantic Salmon Salmo Salar ◽  
Short Term Exposure ◽  
Atrial Natriuretic

To whom correspondence and reprint requests should be addressed. Freshwater Atlantic salmon (Salmo salar L.) smolts were abruptly transferred to sea water in May and over 3 days blood plasma ion concentrations were determined together with atrial natriuretic peptide (ANP) and plasma renin activity (PRA) using antibodies raised against human ANP and angiotensin I. Blood plasma Na+ and Cl− levels in smolts increased and, between 24 and 72 h, PRA increased significantly to 0.9 ng ml−1 h−1, while there was a gradual rise in ANP levels to 10 pmol l−1 at 72 h. Similar measurements were made on parr transferred to sea water in September; in these fish Na+ and Cl− levels increased as in smolts, PRA remained unchanged at about 0.6 ng ml−1 h−1 and ANP levels increased significantly to about 20pmoll−1 at 24 and 72 h. After 2h in sea water parr showed wide variability in ANP levels, in keeping with circulatory stress, hypoxia and increased atrial stretching. Parr transferred to sea water in December showed low drinking rates of 1.95 ml kg−1 h−1, even after 20 days, compared to a high drinking rate of about 7 ml kg−1 h−1 reported for smolts transferred in May. Rainbow trout acclimated to sea water for 3 weeks showed elevated levels of both ANP and PRA, similar to values reported for marine species. Freshwater rainbow trout fed a high-salt diet (12% NaCl) showed significantly elevated levels of ANP compared to fish fed a normal diet (1.5 % NaCl), while PRA levels were depressed, though not significantly.

scholarly journals Effect of Dietary Carbohydrate-to-Protein Ratio on Gut Microbiota in Atlantic Salmon (Salmo salar)

Animals ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 89 ◽  
Author(s):  
Alejandro Villasante ◽  
Carolina Ramírez ◽  
Natalia Catalán ◽  
Rafael Opazo ◽  
Patricio Dantagnan ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Gut Microbiota ◽  
Salmo Salar ◽  
High Throughput Sequencing ◽  
Wheat Starch ◽  
Rrna Gene ◽  
Protein Ratio ◽  
Distal Intestine ◽  
High Carbohydrate ◽  
Atlantic Salmon Salmo Salar

Atlantic salmon (Salmo salar) is a carnivorous fish species whose productive performance tends to be suboptimal when fed low-cost carbohydrate rich meals. It is of interest to study the dynamics of gut microbiota communities in salmonids fed high carbohydrate diets since gut microbes are referred to as key players that influence the metabolism and physiology of the host. A study was conducted to determine the effect of feeding a high carbohydrate diet to Atlantic salmon in gut microbiota communities. A medium carbohydrate (15% wheat starch)/medium protein (MC/MP) diet or a high carbohydrate (30% wheat starch)/low protein (HC/LP) diet was fed to triplicate tanks (28 fish each) during four weeks. We conducted an in-depth characterization of the distal intestine digesta microbiota using high-throughput sequencing of the V4 region of the 16S rRNA gene. Firmicutes, Actinobacteria and Proteobacteria were the major phyla determined in either experimental group. Phylum Planctomycetes, class Planctomycetia, order Planctomycetales and genus Lactococcus were significantly more abundant in fish fed the HC/LP diet compared with fish fed the MC/MP diet. Our study suggests feeding a carbohydrate rich meal to salmon exerts a low impact on the structure of gut microbial communities, affecting mostly low-abundance bacteria capable of metabolizing anaerobically carbohydrates as a major energy-yielding substrate.

scholarly journals The genome of a landlocked Atlantic salmon Salmo salar characterized through high-throughput sequencing

2016 ◽  
Vol 8 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Hauge H. ◽  
K. Dahle M. ◽  
B. Kristoffersen A. ◽  
Grove S. ◽  
R. Wiik-Nielsen C. ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Atlantic Salmon Salmo Salar

Microstructure and texture of fresh and smoked Atlantic salmon, Salmo salar L., fillets from fish reared and slaughtered under different conditions

2001 ◽  
Vol 32 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Sjofn Sigurgisladottir ◽  
Margret S. Sigurdardottir ◽  
Helga Ingvarsdottir ◽  
Ole J. Torrissen ◽  
Hannes Hafsteinsson
Keyword(s):  
Atlantic Salmon ◽  
Salmo Salar ◽  
Atlantic Salmon Salmo Salar
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