scholarly journals Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici

2020 ◽  
Vol 21 (7) ◽  
pp. 2640 ◽  
Author(s):  
Pengfei Zhi ◽  
Lingyao Kong ◽  
Jiao Liu ◽  
Xiaona Zhang ◽  
Xiaoyu Wang ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Histone Deacetylase ◽  
Powdery Mildew Resistance ◽  
Defense Responses ◽  
Blumeria Graminis ◽  
Negative Regulator ◽  
Economic Losses ◽  
Mildew Resistance ◽  
Histone Deacetylase 2 ◽  
Wheat Powdery Mildew

Powdery mildew disease caused by Blumeria graminis f.sp. tritici (Bgt) leads to severe economic losses in bread wheat (Triticum aestivum L.). To date, only a few epigenetic modulators have been revealed to regulate wheat powdery mildew resistance. In this study, the histone deacetylase 2 (HD2) type histone deacetylase TaHDT701 was identified as a negative regulator of wheat defense responses to Bgt. Using multiple approaches, we demonstrated that TaHDT701 associates with the RPD3 type histone deacetylase TaHDA6 and the WD40-repeat protein TaHOS15 to constitute a histone deacetylase complex, in which TaHDT701 could stabilize the TaHDA6-TaHOS15 association. Furthermore, knockdown of TaHDT701, TaHDA6, and TaHOS15 resulted in enhanced wheat powdery mildew resistance, suggesting that the TaHDT701-TaHDA6-TaHOS15 histone deacetylase complex negatively regulates wheat defense responses to Bgt. Moreover, chromatin immunoprecipitation assays revealed that TaHDT701 could function in concert with TaHOS15 to recruit TaHDA6 to the promoters of defense-related genes such as TaPR1, TaPR2, TaPR5, and TaWRKY45. In addition, silencing of TaHDT701, TaHDA6, and TaHOS15 resulted in the up-regulation of TaPR1, TaPR2, TaPR5, and TaWRKY45 accompanied with increased histone acetylation and methylation, as well as reduced nucleosome occupancy, at their promoters, suggesting that the TaHDT701-TaHDA6-TaHOS15 histone deacetylase complex suppresses wheat powdery mildew resistance by modulating chromatin state at defense-related genes.

Assessment of Lipid Transfer Protein (LTP1) Gene in Wheat Powdery Mildew Resistance

2006 ◽  
Vol 5 (4) ◽  
pp. 241-249 ◽  
Author(s):  
Ai-li LI ◽  
Cheng-sheng MENG ◽  
Rong-hua ZHOU ◽  
Zhi-ying MA ◽  
Ji-zeng JIA
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Mildew Resistance ◽  
Transfer Protein ◽  
Wheat Powdery Mildew

scholarly journals Selections from barley landrace collected in Libya as new sources of effective resistance to powdery mildew (Blumeria graminis f.sp. hordei)

2011 ◽  
Vol 48 (No. 5) ◽  
pp. 217-223 ◽  
Author(s):  
J.H. Czembor ◽  
H.J. Czembor
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Agricultural Research ◽  
Genetic Resistance ◽  
Recessive Gene ◽  
Blumeria Graminis ◽  
Hordeum Vulgare L ◽  
Mildew Resistance ◽  
Barley Landrace ◽  
Barley Powdery Mildew

Powdery mildew on barley (Hordeum vulgare L.) caused by the pathogen Blumeria graminis f.sp. hordei occurs worldwide and can result in severe yield loss. Because agronomical methods to control the disease are not completely effective, cultivars with genetic resistance are needed. Therefore, there is a need to describe new sources of genes that confer resistance to barley powdery mildew. This study was conducted to determine the genetic basis of resistance to powdery mildew in three selections 995-1-1, 995-1-2, 995-1-3 from barley landrace 995 (ICB 112840) collected in Al Aziziyah district, Tripolitania, Libya. Landrace originated from InternationalCenter for Agricultural Research in the Dry Areas – ICARDA, Aleppo, Syria. To determine the number of genes, the types of genes action and the gene loci in tested lines two types of crosses were made: (1) the lines were crossed to the susceptible cultivar Pallas, (2) the lines were crossed with Pallas isoline P22 carrying gene mlo5. The parents and progeny F2 were evaluated with isolate R303.1 for the powdery mildew resistance. Based on segregation ratios we found that resistance in these three selections was determined by a single recessive gene allelic to the Mlo locus occurring in Pallas isoline P22. In addition tested lines showed resistance reaction type 0(4) characteristic only for genes mlo. The value of new identified sources of highly effective powdery mildew resistance to breeding programs and barley production is discussed.

scholarly journals Collinearity Analysis and High-Density Genetic Mapping of the Wheat Powdery Mildew Resistance Gene Pm40 in PI 672538

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0164815 ◽  
Author(s):  
Shengfu Zhong ◽  
Lixia Ma ◽  
Syeda Akash Fatima ◽  
Jiezhi Yang ◽  
Wanquan Chen ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Genetic Mapping ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
High Density ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Wheat Powdery Mildew

scholarly journals HvGCN2 silencing in barley displays enhanced Blumeria graminis f. sp. hordei susceptibility

2018 ◽  
Vol 70 (3) ◽  
pp. 513-520
Author(s):  
Ibrahim Ozturk ◽  
Figen Ersoy ◽  
Mahinur Akkaya
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Blumeria Graminis ◽  
Virus Induced Gene Silencing ◽  
Incompatible Interaction ◽  
Resistance Response ◽  
Mildew Resistance ◽  
Barley Powdery Mildew ◽  
Powdery Mildew Disease ◽  
Crop Disease

Powdery mildew disease, caused by Blumeria graminis f. sp. hordei (Bgh), which belongs to the order Erysiphales, is a major crop disease. The general control nondepressible-2 (GCN2) gene of barley was previously found to be overexpressed during the powdery mildew resistance response. Recently, Arabidopsis thaliana GCN2 (AtGCN2) was shown to be involved in disease resistance against biotrophic and necrotrophic pathogens. In order to understand the function of Hordeum vulgare GCN2 (HvGCN2) in the barley powdery mildew resistance response, this gene was silenced by barley stripe mosaic virus (BSMV), mediated by virus-induced gene silencing (VIGS). This is the first study showing the potential importance of HvGCN2 in powdery mildew disease of barley. Based on our observations, when HvGCN2 was silenced on average by 53.5%, Bgh development was increased by 18.7 to 32.1%, which was determined by primary, secondary and longest hyphae measurements. The number of germinated spores also increased 2.8-fold in HvGCN2 silenced plants compared to control plants (BSMV:00). On the other hand, under the resistant condition, no difference was observed in HvGCN2- silenced plants compared to non-silenced lines although the gene was found to be overexpressed in incompatible interaction.

scholarly journals Fine Mapping of Two Wheat Powdery Mildew Resistance Genes Located at the Pm1 Cluster

2016 ◽  
Vol 9 (2) ◽  
Author(s):  
Junchao Liang ◽  
Bisheng Fu ◽  
Wenbin Tang ◽  
Nasr U. Khan ◽  
Na Li ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Genes ◽  
Fine Mapping ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Wheat Powdery Mildew
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