Molecular Tagging Wheat Powdery Mildew Resistance Gene Pm21 by EST-SSR and STS Markers

2010 ◽  
Author(s):  
Xiaolei Qi ◽  
Fa Cui ◽  
Yu Li ◽  
Anming Ding ◽  
Jun Li ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Molecular Tagging ◽  
Wheat Powdery Mildew ◽  
Sts Markers

scholarly journals Collinearity Analysis and High-Density Genetic Mapping of the Wheat Powdery Mildew Resistance Gene Pm40 in PI 672538

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0164815 ◽  
Author(s):  
Shengfu Zhong ◽  
Lixia Ma ◽  
Syeda Akash Fatima ◽  
Jiezhi Yang ◽  
Wanquan Chen ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Genetic Mapping ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
High Density ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Wheat Powdery Mildew

scholarly journals NLR1-V, a CC-NBS-LRR encoding gene, is a potential candidate gene of the wheat powdery mildew resistance gene Pm21

2017 ◽  
Author(s):  
Liping Xing ◽  
Ping Hu ◽  
Jiaqian Liu ◽  
Chaofan Cui ◽  
Hui Wang ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Candidate Gene ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Potential Candidate ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Wheat Powdery Mildew ◽  
Potential Candidate Gene ◽  
Encoding Gene

AbstractWheat powdery mildew caused by Blumeria graminisb f. sp. tritici is one of the most destructive diseases all over the world. Pm21, transferred from the wild Haynaldia villosa to wheat, confers broad spectrum resistance throughout the whole stage, and this gene has been widely used in wheat production for more than 20 years. Cloning the candidate gene of Pm21 is the prerequisite for elucidating the resistance mechanism, and is a valuable attempt to clone the target genes from the evolutionarily distant wild species. In this study, an innovative approach, which combined cytogenetic stocks development, mutagenesis, RenSeq and PacBio, was tried successfully to clone an NBS-LRR type gene NLR1-V from the Pm21 locus. Firstly, a powdery mildew resistant cryptic alien introgression line HP33 involved very small 6VS segment was developed, and 6 independent susceptible mutants of T6VS · 6AL was identified. Then, the transcriptome of H. villosa was obtained by NGS and the full-length NBS-LRR gene database was constructed by RenSeq-PacBio. In the following study, two expressed NLR genes were located to the Pm21 locus using the HP33 as the mapping material, and only NLR1-V showed polymorphism between the wild T6VS · 6AL and its six mutants. The functional analysis indicated that silencing of NLR1-V could compromise the resistance of T6VS · 6AL completely, and could also decrease the resistance of T6VS · 6DL dramatically. Moreover, NLR1-V could recover the resistance of the susceptible mutant and increase the resistance in the susceptible wheat. The study implied that NLR1-V, a CC-NBS-LRR encoding gene, is a potential candidate gene of the powdery mildew resistance gene Pm21.

STS markers for powdery mildew resistance gene Pm6 in wheat

Euphytica ◽  
2007 ◽  
Vol 163 (2) ◽  
pp. 159-165 ◽  
Author(s):  
Jianhui Ji ◽  
Bi Qin ◽  
Haiyan Wang ◽  
Aizhong Cao ◽  
Suling Wang ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Sts Markers
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