scholarly journals Fibroblast Interaction with Different Abutment Surfaces: In Vitro Study

2020 ◽  
Vol 21 (6) ◽  
pp. 1919 ◽  
Author(s):  
Luigi Canullo ◽  
Tullio Genova ◽  
Esperanza Gross Trujillo ◽  
Guillermo Pradies ◽  
Sara Petrillo ◽  
...  
Keyword(s):  
Ultraviolet Light ◽  
Early Stage ◽  
Argon Plasma ◽  
In Vitro Study ◽  
Side Surface ◽  
Cellular Adhesion ◽  
Grade 5 ◽  
Tissue Healing ◽  
Titanium Surfaces

Background: Attaining an effective mucosal attachment to the transmucosal part of the implant could protect the peri-implant bone. Aim: To evaluate if chair side surface treatments (plasma of Argon and ultraviolet light) may affect fibroblast adhesion on different titanium surfaces designed for soft tissue healing. Methods: Grade 5 titanium discs with four different surface topographies were subdivided into 3 groups: argon-plasma; ultraviolet light, and no treatment. Cell morphology and adhesion tests were performed at 20 min, 24 h, and 72 h. Results: Qualitative observation of the surfaces performed at the SEM was in accordance with the anticipated features. Roughness values ranged from smooth (MAC Sa = 0.2) to very rough (XA Sa = 21). At 20 min, all the untreated surfaces presented hemispherical cells with reduced filopodia, while the cells on treated samples were more spread with broad lamellipodia. However, these differences in spreading behavior disappeared at 24 h and 72 h. Argon-plasma, but not UV, significantly increased the number of fibroblasts independently of the surface type but only at 20 min. Statistically, there was no surface in combination with a treatment that favored a greater cellular adhesion. Conclusions: Data showed potential biological benefits of treating implant abutment surfaces with the plasma of argon in relation to early-stage cell adhesion.

scholarly journals Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 654
Author(s):  
Roberta Di Carlo ◽  
Antonello Di Crescenzo ◽  
Serena Pilato ◽  
Alessia Ventrella ◽  
Adriano Piattelli ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Osteogenic Differentiation ◽  
Early Stage ◽  
In Vitro Study ◽  
Titanium Implant ◽  
Osteoblastic Differentiation ◽  
Host Tissue ◽  
Cytotoxic Response ◽  
Titanium Surfaces

Background: Titanium implant surfaces are continuously modified to improve biocompatibility and to promote osteointegration. Graphene oxide (GO) has been successfully used to ameliorate biomaterial performances, in terms of implant integration with host tissue. The aim of this study is to evaluate the Dental Pulp Stem Cells (DPSCs) viability, cytotoxic response, and osteogenic differentiation capability in the presence of GO-coated titanium surfaces. Methods: Two titanium discs types, machined (control, Crtl) and sandblasted and acid-etched (test, Test) discs, were covalently functionalized with GO. The ability of the GO-functionalized substrates to allow the proliferation and differentiation of DPSCs, as well as their cytotoxic potential, were assessed. Results: The functionalization procedures provide a homogeneous coating with GO of the titanium surface in both control and test substrates, with unchanged surface roughness with respect to the untreated surfaces. All samples show the deposition of extracellular matrix, more pronounced in the test and GO-functionalized test discs. GO-functionalized test samples evidenced a significant viability, with no cytotoxic response and a remarkable early stage proliferation of DPSCs cells, followed by their successful differentiation into osteoblasts. Conclusions: The described protocol of GO-functionalization provides a novel not cytotoxic biomaterial that is able to stimulate cell viability and that better and more quickly induces osteogenic differentiation with respect to simple titanium discs. Our findings pave the way to exploit this GO-functionalization protocol for the production of novel dental implant materials that display improved integration with the host tissue.

Spreading of Epithelial Cells on Machined and Sandblasted Titanium Surfaces: An In Vitro Study

2003 ◽  
Vol 74 (3) ◽  
pp. 289-295 ◽  
Author(s):  
Maristella Di Carmine ◽  
Paola Toto ◽  
Claudio Feliciani ◽  
Antonio Scarano ◽  
Antonello Tulli ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Titanium Surfaces

scholarly journals Enzyme-treated Asparagus Extract (ETAS) Enhances Memory in Normal Rats and Induces Neurite-outgrowth in PC12 Cells

2017 ◽  
Vol 12 (10) ◽  
pp. 1934578X1701201
Author(s):  
Tomoko Koda ◽  
Jun Takanari ◽  
Kentaro Kitadate ◽  
Hideki Imai
Keyword(s):  
Cognitive Impairment ◽  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Early Stage ◽  
Memory Function ◽  
In Vitro Study ◽  
Accelerated Learning ◽  
Samp8 Mice

Enzyme-treated asparagus extract (ETAS) contains various phytochemicals. It has been reported that ETAS protected early cognitive impairment of SAMP8 mice and Aβ-induced reduction of PC12 cell viability. In addition, ETAS had several functions on healthy people. The purpose of the present study was to investigate the effects of ETAS on normal memory function of rats and neurite-outgrowth of PC12 cells. ETAS significantly accelerated learning acquisition in in vivo and neurite-outgrowth in in vitro study. In conclusion, ETAS affected on memory function in early stage in normal rats. The basis of this effect may induce neurite-outgrowth in neurons.

Osteoblast response to puerarin-loaded porous titanium surfaces: An in vitro study

2012 ◽  
Vol 100A (6) ◽  
pp. 1419-1426 ◽  
Author(s):  
Fan Yang ◽  
Rong Zhang ◽  
Fuming He ◽  
Xiao-Xiang Wang ◽  
Shifang Zhao ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Porous Titanium ◽  
Vitro Study ◽  
Titanium Surfaces

scholarly journals Microbiological and SEM-EDS Evaluation of Titanium Surfaces Exposed to Periodontal Gel: In Vitro Study

Materials ◽  
2019 ◽  
Vol 12 (9) ◽  
pp. 1448 ◽  
Author(s):  
Sara Bernardi ◽  
Serena Bianchi ◽  
Anna Rita Tomei ◽  
Maria Adelaide Continenza ◽  
Guido Macchiarelli
Keyword(s):  
Inflammatory Diseases ◽  
Test Group ◽  
In Vitro Study ◽  
Positive Control ◽  
Control Group ◽  
Anaerobic Conditions ◽  
Titanium Surfaces ◽  
Implant Dentistry

Inflammatory diseases affecting the soft and hard tissues surrounding an implant represent a new challenge in contemporary implant dentistry. Among several methods proposed for the decontamination of titanium surfaces, the administration of topical 14% doxycycline gel seems to be a reliable option. In the present study, we evaluated the microbial effect of 14% doxycycline gel applied on titanium surfaces and exposed to human salivary microbes in anaerobic conditions. We also examined the composition of the exposed surfaces to assess the safe use of periodontal gel on titanium surfaces. Six anatase and six type 5 alloy titanium surfaces were used and divided into two groups: The test group and the positive control group. Both were cultured with human salivary samples in anaerobic conditions. On the test groups, 240 mg of periodontal gel was applied. The microbial assessment was performed with a colony-forming unit (CFU) count and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) to identify the species. The surface integrity was assessed using scanning electron microscopy-energy dispersive X-ray spectrometry (SEM-EDS). The results demonstrated the microbial efficacy of the 14% doxycycline periodontal gel and its safe use on titanium surfaces. However, the SEM observations revealed the permanence of the gel on the titanium surfaces due to the physical composition of the gel. This permanence needs to be further investigated in vivo and a final polishing protocol on the titanium surface is recommended.

Comparison of the spine kinematics by defining lumbar as single and multi-segmental in completing critical daily task

2020 ◽  
Vol 14 (4) ◽  
pp. 7600-7608
Author(s):  
W. A. A Saad ◽  
Mohd Azuwan Mat Dzahir ◽  
Yamamoto Shinichirou ◽  
Mohamed Hussein ◽  
Maziah Mohamad ◽  
...  
Keyword(s):  
Independent Living ◽  
Early Stage ◽  
In Vitro Study ◽  
Spinal Curvature ◽  
Invasive Approach ◽  
Motion Patterns ◽  
Non Invasive ◽  
Spine Kinematics ◽  
Significant Difference

The change of the spinal curvature in completing a variety of daily tasks is essential to independent living. There is still a lack of studies highlighting the lumbar segmental contribution during sit-to-stand (STS) and stand-to-flexion (STF) using non-invasive study. The purpose of this study is to compare the spine kinematics by defining lumbar as a single and multi-segmental during continuous daily motion in healthy Asian adults using a non-invasive approach. During STS, most subjects implemented kyphotic lumbar curve during the early stage of motion which revealed poor posture implementation and significant differences in the lumbar kinematics which were only noticeable at specific phases between both approaches. A significant difference in multi-segmental behaviour was observed only at the end of the motion. All three segments displayed different time responses during the transition from kyphotic to lordotic curve. Passive/delayed behavior within the lower lumbar segment was observed between 0-50% of motion completion. During STF, statistically significant differences were found between assuming lumbar as a single and multi-segment in all phases. This in vitro study identified characteristic motion patterns in the lumbar spine during daily motions. The results provided a clear description of the healthy spinal condition of adults and may serve to identify specific multi-segmental contribution.

scholarly journals Assessing adhesive remnant removal and enamel damage with ultraviolet light: An in-vitro study

2017 ◽  
Vol 151 (2) ◽  
pp. 292-296 ◽  
Author(s):  
Alexandre Antonio Ribeiro ◽  
Layene Figueiredo Almeida ◽  
Lidia Parsekian Martins ◽  
Renato Parsekian Martins
Keyword(s):  
Ultraviolet Light ◽  
In Vitro Study ◽  
Vitro Study
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