scholarly journals Distinct Metabolome Changes during Seed Germination of Lettuce (Lactuca sativa L.) in Response to Thermal Stress as Revealed by Untargeted Metabolomics Analysis

2020 ◽  
Vol 21 (4) ◽  
pp. 1481 ◽  
Author(s):  
Shiwei Wei ◽  
Xiao Yang ◽  
Guotao Huo ◽  
Guojun Ge ◽  
Hongyan Liu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Thermal Stress ◽  
Seed Germination ◽  
Organic Acids ◽  
Lactuca Sativa ◽  
Sesquiterpene Lactones ◽  
Germination Percentage ◽  
Untargeted Metabolomics ◽  
Lettuce Seed ◽  
Lactuca Sativa L

Temperature strongly influences lettuce (Lactuca sativa L.) seed germination. Different lettuce genotypes respond differently to higher temperatures or thermal stress. In this study, we evaluated the germination performance of 304 lettuce accessions incubated at three temperature settings, 21 °C, 28 °C and 35 °C, respectively, for 40 h. At 21 °C, seeds of all 304 accessions germinated with very well an average germination percentage of 87.72%; at 28 °C, the average germination percentage dropped to 42.84% and at 35 °C, the germination decreased to 1.01%. Then, we investigated changes in metabolic profiles of lettuce seed response to thermal stress using an untargeted metabolomics approach. Results suggested that seeds of thermal-sensitive and thermal-tolerant cultivars employed different metabolic strategies in response to thermal stress during germination. Thermal-sensitive buds accumulated more significant amounts of organic acids, amino acids, sugars, sterols, phenolic compounds and terpenoids compared to thermal-tolerant buds at 21 °C. Thermal-tolerant lettuce cultivar accumulated higher concentrations of amino acids, organic acids, sugars, sesquiterpene lactones, sterols, and fatty acids derivatives during the germination at 35 °C compared to germinated at 21 °C. This investigation paves the way to link the metabolomics to other external and internal factors affecting lettuce seed germination under thermal stress.

scholarly journals Preventing thermoinhibition in a thermosensitive lettuce genotype by seed imbibition at low temperature

2003 ◽  
Vol 60 (3) ◽  
pp. 477-480 ◽  
Author(s):  
Warley Marcos Nascimento
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
High Temperatures ◽  
Lactuca Sativa ◽  
Lettuce Seed ◽  
Temperature Dependent ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Lettuce Seed Germination ◽  
Dark Green

Lettuce (Lactuca sativa L.) seed germination is strongly temperature dependent and under high temperatures, germination of most of genotypes can be erratic or completely inhibited. Lettuce seeds of 'Dark Green Boston' (DGB) were incubated at temperatures ranging from 15° to 35°C at light and dark conditions. Other seeds were imbibed in dark at 20°; 25°; 30°; and 35°C for 8 and 16 hours and then transferred to 20 or 35°C, in dark. Seeds were also incubated at constant temperature of 20° and 35 °C, in the dark, as control. In another treatment, seeds were primed for 3 days at 15°C with constant light. DGB lettuce seeds required light to germinate adequately at temperatures above 25°C. Seeds incubated at 20°C had 97% germination, whereas seeds incubated at 35°C did not germinate. Seeds imbibed at 20°C for 8 and 16 hours had germination. At 35°C, seeds imbibed initially at 20°C for 8 and 16 hours, had 89 and 97% germination, respectively. Seeds imbibed at 25°C for 16 hours, germinated satisfactory at 35°C. High temperatures of imbibition led to no germination. Primed and non-primed seeds had 100% germination at 20°C. Primed seeds had 100% germination at 35°C, whereas non-primed seeds germinate only 4%. The first hours of imbibition are very critical for lettuce seed germination at high temperatures.

Isolation and characterisation of the sesquiterpene lactones from Lactuca sativa L var. anagustata

2010 ◽  
Vol 120 (4) ◽  
pp. 1083-1088 ◽  
Author(s):  
Yi-Feng Han ◽  
Gui-Xiu Cao ◽  
Xiao-Jing Gao ◽  
Min Xia
Keyword(s):  
Lactuca Sativa ◽  
Sesquiterpene Lactones ◽  
Lactuca Sativa L

Variation of Sesquiterpene Lactones Content in Lettuce (Lactuca sativa L.) Germplasm

2016 ◽  
Vol 28 (2) ◽  
pp. 220-226 ◽  
Author(s):  
Gyu-Taek Cho ◽  
◽  
Young-Yi Lee ◽  
Susanna Choi ◽  
Jung-Yoon Yi ◽  
...  
Keyword(s):  
Lactuca Sativa ◽  
Sesquiterpene Lactones ◽  
Lactuca Sativa L

scholarly journals GERMINATION AND DEVELOPMENT OF AMERICAN LETTUCE SEEDLINGS (Lactuca sativa L.) IRRIGATED WITH HOME AND INDUSTRIAL EFFLUENT-RECEIVING WATER

Irriga ◽  
2004 ◽  
Vol 9 (3) ◽  
pp. 207-216
Author(s):  
Guilherme Augusto Biscaro ◽  
Rodrigo De Menezes Trigueiro ◽  
Raimundo Leite Cruz ◽  
Marcelo Domingos Chamma Lopes
Keyword(s):  
Lactuca Sativa ◽  
Industrial Effluent ◽  
Germination Percentage ◽  
Final Size ◽  
Mato Grosso ◽  
Lactuca Sativa L ◽  
Creek Water ◽  
Engineering Department ◽  
Number Of Leaves ◽  
Receiving Water

GERMINATION AND DEVELOPMENT OF AMERICAN LETTUCE SEEDLINGS (Lactuca sativa L.) IRRIGATED  WITH HOME AND INDUSTRIAL EFFLUENT-RECEIVING WATER   Guilherme Augusto Biscaro1; Rodrigo de Menezes Trigueiro2; Raimundo Leite Cruz2; Marcelo Domingos Chamma Lopes2State University of Mato Grosso do Sul, Cassilândia, MS, (Brazil), [email protected] Engineering Department, Agronomic Science College,Paulista State University ,  Botucatu-SP (Brazil).   1 ABSTRACT  This work has evaluated germination and formation of American lettuce seedlings (Lactuca sativa L.) “Tainá” variety, irrigated with urban effluent-receiving water from Lavapés Creek,  a water body which receives all home and industrial sewage of the city of  Botucatu, SP (Brazil). The sowing was carried out in 12 plastic foam trays with 32 germination cells each. At 41 days after planting (41-DAP), the seed germination percentage, seedling final size, final number of leaves, the mass of shoot and root dry matter were evaluated. Also, two sorts of substrates were investigated: Multiplantâ and Plug Mixâ. No complementary fertilization was done, so the only available nutrients were the ones  originally present in the substrates and in the creek water. For compararison an evaluation was performed using a source of treated water instead of the creek water. Results pointed out that all sown seeds presented 100% of germination. Treatments with  Multiplantâ presented the greatest values for plant height. Treatments irrigated with Lavapés  Creek water presented a greater number of leaves at 41-DAP.  KEYWORDS: Seedling germination, Lactuca sativa L., effluent receiving water.   BISCARO, G. A.; TRIGUEIRO, R. M.; CRUZ, R. L.; LOPES, M. D. C. GERMINAÇÃO E DESENVOLVIMENTO DE MUDAS DE ALFACE AMERICANA (Lactuca sativa L.) IRRIGADOS COM ÁGUAS RECEPTORAS DE EFLUENTES DOMÉSTICOS E INDUSTRIAIS   2 RESUMO  O trabalho avaliou a germinação e a formação de mudas de alface americana (Lactuca sativa L.) variedade “Tainá”, irrigada com águas receptoras de efluentes urbanos do Ribeirão Lavapés, curso d’água que recebe todo o esgoto doméstico e industrial da cidade de Botucatu – SP (Brasil). A semeadura foi realizada em 12 bandejas de isopor com 32 células de germinação cada. Foram analisados com 41 dias após o plantio (41-DAP), a porcentagem de germinação das sementes, o tamanho final das mudas, o número final de folhas, a massa de matéria seca da parte aérea e de raízes. Também foram estudados dois tipos de substrato: Multiplantâ e Plug Mixâ. Não foi utilizada nenhuma adubação complementar, estando disponível apenas os nutrientes presentes originalmente nos substratos e na água do ribeirão. Para efeito comparativo, realizou-se a mesma avaliação utilizando uma fonte de água tratada. Os resultados indicaram que todas as células semeadas apresentaram 100% de germinação. Os tratamentos que utilizaram o Multiplantâ apresentaram maiores valores de altura de plantas e os irrigados com a água do Ribeirão Lavapés, apresentaram maior número de folhas com 41-DAP.  UNITERMOS: Germinação de mudas, Lactuca sativa L., águas receptoras de efluentes.

The effects of ultra-high diluted gibberellic acid on in vitro lettuce seed germination.

2021 ◽  
Vol 15 (4) ◽  
pp. 50-54
Author(s):  
Carolina Santos Barreto ◽  
Fortune Homsani ◽  
Nina C Barboza Da Silva ◽  
Carla Holandino
Keyword(s):  
Gibberellic Acid ◽  
Lactuca Sativa ◽  
Germination Rate ◽  
Petri Dish ◽  
New Drugs ◽  
Lettuce Seed ◽  
Lettuce Seeds ◽  
Lactuca Sativa L ◽  
Petri Dishes

Lettuce seeds bioassays have been used in many different tests such as: alellopathyc models; developing of new drugs; ecotoxicity tests. In most cases, lettuce (Lactuca sativa L., Asteraceae) has been used because of its sensitivity, simultaneous and rapid germination, reliability of germination percentage and homogeneity of seeds. The main goal was to evaluate the effects of ultra-high diluted gibberellic acid (GA3) on lettuce seeds germination and seedling growth. Experiment was performed using Petri dishes containing one disk of Whatman nº01 paper watered with 1ml of water. In each Petri dish 10 lettuce seeds(Lactuca sativa L.) cv Regina 500 were placed and 2ml of the different treatment solutions were add: GA33µmol, GA3 3CH (10-6), GA3 12CH (10-24), water 12CH and water (no dilution and succussion). One milliliter solutions were added every 2 days of experiment. The experiment was repeated twice and each one consisted in 5 Petri dishes per treatment (n=100). All seeds were maintained in germination incubator under controlled temperature (25°C) and photoperiod (16L/8D). The tested substances were prepared according to Brazilian Homeopathic Pharmacopoeia (Brazil, 2011). The experiment was blinded all the time. All seeds germinated at same time (2 days) and after 7 days the germination rate was the same in all treatments. Root was affected just by Water 12 CH, in which shown the longest length (4.59 cm) when compared with others treatments. Shoot length was higher where gibberellin was added in concentration upper then Avogrado’s number.

Sign in / Sign up

Export Citation Format

Share Document