scholarly journals Identification and Molecular Characterization of a Pellino Protein in Kuruma Prawn (Marsupenaeus Japonicus) in Response to White Spot Syndrome Virus and Vibrio Parahaemolyticus Infection

2020 ◽  
Vol 21 (4) ◽  
pp. 1243
Author(s):  
Heqian Zhang ◽  
Wenzhi Cheng ◽  
Jinbin Zheng ◽  
Panpan Wang ◽  
Qinghui Liu ◽  
...  
Keyword(s):  
Immune Response ◽  
Vibrio Parahaemolyticus ◽  
White Spot Syndrome Virus ◽  
Transcript Level ◽  
Protein Docking ◽  
Shrimp Farming ◽  
White Spot ◽  
Marsupenaeus Japonicus ◽  
White Spot Syndrome

Kuruma prawn, Marsupenaeus japonicus, has the third largest annual yield among shrimp species with vital economic significance in China. White spot syndrome virus (WSSV) is a great threat to the global shrimp farming industry and results in high mortality. Pellino, a highly conserved E3 ubiquitin ligase, has been found to be an important modulator of the Toll-like receptor (TLR) signaling pathways that participate in the innate immune response and ubiquitination. In the present study, the Pellino gene from Marsupenaeus japonicus was identified. A qRT-PCR assay showed the presence of MjPellino in all the tested tissues and revealed that the transcript level of this gene was significantly upregulated in both the gills and hemocytes after challenge with WSSV and Vibrio parahaemolyticus. The function of MjPellino was further verified at the protein level. The results of the three-dimensional modeling and protein–protein docking analyses and a GST pull-down assay revealed that the MjPellino protein was able to bind to the WSSV envelope protein VP26. In addition, the knockdown of MjPellino in vivo significantly decreased the expression of MjAMPs. These results suggest that MjPellino might play an important role in the immune response of kuruma prawn.

scholarly journals Identification of antigenic domains and peptides from VP15 of white spot syndrome virus and their antiviral effects in Marsupenaeus japonicus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jirayu Boonyakida ◽  
Jian Xu ◽  
Jun Satoh ◽  
Takafumi Nakanishi ◽  
Tohru Mekata ◽  
...  
Keyword(s):  
Survival Rate ◽  
White Spot Syndrome Virus ◽  
Expression System ◽  
Penaeid Shrimp ◽  
White Spot ◽  
Marsupenaeus Japonicus ◽  
Structural Protein ◽  
E Coli ◽  
White Spot Syndrome

AbstractWhite spot syndrome virus (WSSV) is one of the most devastating pathogens in penaeid shrimp and can cause massive damage in shrimp aquaculture industries. Previously, the WSSV structural protein VP15 was identified as an antigenic reagent against WSSV infections. In this study, we truncated this protein into VP15(1–25), VP15(26–57), VP15(58–80), and VP15(1–25,58–80). The purified proteins from the E. coli expression system were assayed as potential protective agents in Kuruma shrimp (Marsupenaeus japonicus) using the prime-and-boost strategy. Among the four truncated constructs, VP15(26–57) provided a significant improvement in the shrimp survival rate after 20 days of viral infection. Subsequently, four peptides (KR11, SR11, SK10, and KK13) from VP15(26–57) were synthesized and applied in an in vivo assay. Our results showed that SR11 could significantly enhance the shrimp survival rate, as determined from the accumulated survival rate. Moreover, a multiligand binding protein with a role in the host immune response and a possible VP15-binding partner, MjgC1qR, from the host M. japonicus were employed to test its binding with the VP15 protein. GST pull-down assays revealed that MjgC1qR binds with VP15, VP15(26–57), and SR11. Taken together, we conclude that SR11 is a determinant antigenic peptide of VP15 conferring antiviral activity against WSSV.

scholarly journals Identification of Antigenic Domains and Peptides from VP15 of White Spot Syndrome Virus and their Antiviral Effects in Marsupenaeus Japonicus

2021 ◽  
Author(s):  
Jirayu Boonyakida ◽  
Jian Xu ◽  
Jun Satoh ◽  
Takafumi Nakanishi ◽  
Toru Mekata ◽  
...  
Keyword(s):  
Survival Rate ◽  
White Spot Syndrome Virus ◽  
Expression System ◽  
Penaeid Shrimp ◽  
White Spot ◽  
Marsupenaeus Japonicus ◽  
Structural Protein ◽  
E Coli ◽  
White Spot Syndrome

Abstract White spot syndrome virus (WSSV) is one of the most devastating pathogens in penaeid shrimp and can cause massive damage in shrimp aquaculture industries. Previously, the WSSV structural protein VP15 was identified as an antigenic reagent against WSSV infections. In this study, we truncated this protein into VP15(1−25), VP15(26−57), VP15(58−80), and VP15(1−25,58−80). The purified proteins from the E. coli expression system were assayed as potential protective agents in Kuruma shrimp (Marsupenaeus japonicus) using the prime-and-boost strategy. Among the four truncated constructs, VP15(26−57) provided a significant improvement in the shrimp survival rate after 20 days of viral infection. Subsequently, four peptides (KR11, SR11, SK10, and KK13) from VP15(26−57) were synthesized and applied in an in vivo assay. Our results showed that SR11 could significantly enhance the shrimp survival rate, as determined from the accumulated survival rate. Moreover, a multiligand binding protein with a role in the host immune response and a possible VP15-binding partner, MjgC1qR, from the host M. japonicus were employed to test its binding with the VP15 protein. GST pull-down assays revealed that MjgC1qR binds with VP15, VP15(26−57), and SR11. Taken together, we conclude that SR11 is a determinant antigenic peptide of VP15 conferring antiviral activity against WSSV.

scholarly journals Phycoerythrin from Colaconema sp. Has Immunostimulatory Effects on the Whiteleg Shrimp Litopenaeus vannamei and Increases Resistance to Vibrio parahaemolyticus and White Spot Syndrome Virus

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2371
Author(s):  
Po-Tsang Lee ◽  
Jing Huang ◽  
Chin-Yi Huang ◽  
Zi-Xuan Liu ◽  
Han-Yang Yeh ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
White Spot Syndrome Virus ◽  
Expression Profiles ◽  
White Spot ◽  
Shrimp Culture ◽  
White Spot Syndrome ◽  
Whiteleg Shrimp

We investigated whether phycoerythrin (PE), a pigment sourced from marine algae, could act as an immunomodulatory agent in whiteleg shrimp (Litopenaeus vannamei). To this end, PE was extracted and purified from a PE-rich macroalgae, Colaconema sp. Our in vitro analysis demonstrated that PE enhanced prophenoloxidase and phagocytosis activity but inhibited the production of reactive oxygen species in hemocytes. Additionally, the PE signal could be detected using an in vivo imaging system after its injection into the ventral sinus of the cephalothorax of whiteleg shrimp. The expression profiles of fourteen immune-related genes were monitored in hemocytes from whiteleg shrimp injected with 0.30 μg of PE per gram of body weight, and crustin, lysozyme, penaiedin 4, and anti-lipopolysaccharide factor showed up-regulated post-stimulation. The induction of immune genes and enhancement of innate immune parameters by PE may explain the higher survival rates for shrimp that received different doses of PE prior to being challenged with Vibrio parahaemolyticus or white spot syndrome virus compared to controls. Combined, these results show that PE from Colaconema sp. can differentially stimulate the immune response of whiteleg shrimp in vitro and in vivo and could potentially be used as an immunomodulator in shrimp culture.

scholarly journals Draft Genome Sequence of White Spot Syndrome Virus Isolated from Cultured Litopenaeus vannamei in Mexico

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Libia Zulema Rodriguez-Anaya ◽  
Jose Reyes Gonzalez-Galaviz ◽  
Ramón Casillas-Hernandez ◽  
Fernando Lares-Villa ◽  
Karel Estrada ◽  
...  
Keyword(s):  
Disease Prevention ◽  
Genome Sequence ◽  
Litopenaeus Vannamei ◽  
White Spot Syndrome Virus ◽  
Draft Genome ◽  
Epidemiological Studies ◽  
Shrimp Farming ◽  
White Spot ◽  
Molecular Diagnostic ◽  
White Spot Syndrome

The first genome sequence of a Mexican white spot syndrome virus is presented here. White spot syndrome is a shrimp pandemic virus that has devastated production in Mexico for more than 10 years. The availability of this genome will greatly aid epidemiological studies worldwide, contributing to the molecular diagnostic and disease prevention in shrimp farming.

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