scholarly journals Studies on nitric oxide synthase activity in haemocytes of shrimps Fenneropenaeus chinensis and Marsupenaeus japonicus after white spot syndrome virus infection

Nitric Oxide ◽  
2006 ◽  
Vol 14 (3) ◽  
pp. 219-227 ◽  
Author(s):  
Guojian Jiang ◽  
Rencheng Yu ◽  
Mingjiang Zhou
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Virus Infection ◽  
White Spot Syndrome Virus ◽  
Fenneropenaeus Chinensis ◽  
White Spot ◽  
Marsupenaeus Japonicus ◽  
White Spot Syndrome ◽  
Oxide Synthase ◽  
Nitric Oxide Synthase Activity

scholarly journals WSV056 Inhibits Shrimp Nitric Oxide Synthase Activity by Downregulating Litopenaeus vannamei Sepiapterin Reductase to Promote White Spot Syndrome Virus Replication

2021 ◽  
Vol 12 ◽  
Author(s):  
Wei Wang ◽  
Qin Zheng ◽  
Chen Yu ◽  
Changkun Pan ◽  
Peng Luo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Litopenaeus Vannamei ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
No Production ◽  
Sepiapterin Reductase ◽  
Copy Numbers ◽  
White Spot Syndrome ◽  
Oxide Synthase

Sepiapterin reductase (Spr) plays an essential role in the biosynthesis of tetrahydrobiopterin (BH4), a key cofactor of multiple enzymes involved in various physiological and immune processes. Suppression of Spr could result in BH4 deficiency-caused diseases in human and murine models. However, information on the biological function of Spr in invertebrates is limited. In this study, two Sprs (CG12116 and Sptr) from Drosophila melanogaster were found to be downregulated in transgenic flies overexpressing white spot syndrome virus (WSSV) immediate-early protein WSV056. CG12116 and Sptr exerted an inhibitory effect on the replication of the Drosophila C virus. A Litopenaeus vannamei Spr (LvSpr) exhibiting similarity of 64.1–67.5% and 57.3–62.2% to that of invertebrate and vertebrate Sprs, respectively, were cloned. L. vannamei challenged with WSSV revealed a significant decrease in LvSpr transcription and Spr activity in hemocytes. In addition, the BH4 co-factored nitric oxide synthase (Nos) activity in shrimp hemocytes was reduced in WSSV-infected and LvSpr knockdown shrimp, suggesting WSSV probably inhibits the LvNos activity through LvSpr downregulation to limit the production of nitric oxide (NO). Knockdown of LvSpr and LvNos caused the reduction in NO level in hemocytes and the increase of viral copy numbers in WSSV-infected shrimp. Supplementation of NO donor DETA/NO or double gene knockdown of WSV056 + LvSpr and WSV056 + LvNos recovered the NO production, whereas the WSSV copy numbers were decreased. Altogether, the findings demonstrated that LvSpr and LvNos could potentially inhibit WSSV. In turn, the virus has evolved to attenuate NO production via LvSpr suppression by WSV056, allowing evasion of host antiviral response to ensure efficient replication.

A novel viral responsive protein (MjVRP) from Marsupenaeus japonicus haemocytes is involved in white spot syndrome virus infection

2017 ◽  
Vol 70 ◽  
pp. 638-647 ◽  
Author(s):  
Samia Elbahnaswy ◽  
Keiichiro Koiwai ◽  
Viola H. Zaki ◽  
Adel A. Shaheen ◽  
Hidehiro Kondo ◽  
...  
Keyword(s):  
Virus Infection ◽  
White Spot Syndrome Virus ◽  
White Spot ◽  
Marsupenaeus Japonicus ◽  
White Spot Syndrome

Effect of white spot syndrome virus infection on a Ras gene in the Chinese shrimp Fenneropenaeus chinensis

Aquaculture ◽  
2020 ◽  
Vol 516 ◽  
pp. 734604
Author(s):  
Xupeng Li ◽  
Xianhong Meng ◽  
Sheng Luan ◽  
Kun Luo ◽  
Baoxiang Cao ◽  
...  
Keyword(s):  
Virus Infection ◽  
White Spot Syndrome Virus ◽  
Fenneropenaeus Chinensis ◽  
White Spot ◽  
Ras Gene ◽  
Chinese Shrimp ◽  
White Spot Syndrome
Sign in / Sign up

Export Citation Format

Share Document