scholarly journals Overexpression of a Malus baccata NAC Transcription Factor Gene MbNAC25 Increases Cold and Salinity Tolerance in Arabidopsis

2020 ◽  
Vol 21 (4) ◽  
pp. 1198 ◽  
Author(s):  
Deguo Han ◽  
Man Du ◽  
Zhengyi Zhou ◽  
Shuang Wang ◽  
Tiemei Li ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Stress Responses ◽  
Apical Meristem ◽  
High Salinity ◽  
Transgenic Arabidopsis ◽  
Wild Type ◽  
Mda Content ◽  
High Salt Stress ◽  
Malus Baccata ◽  
Nac Family

NAC (no apical meristem (NAM), Arabidopsis thaliana transcription activation factor (ATAF1/2) and cup shaped cotyledon (CUC2)) transcription factors play crucial roles in plant development and stress responses. Nevertheless, to date, only a few reports regarding stress-related NAC genes are available in Malus baccata (L.) Borkh. In this study, the transcription factor MbNAC25 in M. baccata was isolated as a member of the plant-specific NAC family that regulates stress responses. Expression of MbNAC25 was induced by abiotic stresses such as drought, cold, high salinity and heat. The ORF of MbNAC25 is 1122 bp, encodes 373 amino acids and subcellular localization showed that MbNAC25 protein was localized in the nucleus. In addition, MbNAC25 was highly expressed in new leaves and stems using real-time PCR. To analyze the function of MbNAC25 in plants, we generated transgenic Arabidopsis plants that overexpressed MbNAC25. Under low-temperature stress (4 °C) and high-salt stress (200 mM NaCl), plants overexpressing MbNAC25 enhanced tolerance against cold and drought salinity conferring a higher survival rate than that of wild-type (WT). Correspondingly, the chlorophyll content, proline content, the activities of antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were significantly increased, while malondialdehyde (MDA) content was lower. These results indicated that the overexpression of MbNAC25 in Arabidopsis plants improved the tolerance to cold and salinity stress via enhanced scavenging capability of reactive oxygen species (ROS).

scholarly journals Potato NAC Transcription Factor StNAC053 Enhances Salt and Drought Tolerance in Transgenic Arabidopsis

2021 ◽  
Vol 22 (5) ◽  
pp. 2568
Author(s):  
Qi Wang ◽  
Cun Guo ◽  
Zhiyuan Li ◽  
Jinhao Sun ◽  
Zhichao Deng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Stress Responses ◽  
Nac Transcription Factor ◽  
Transactivation Domain ◽  
Wild Type ◽  
Gfp Fusion Protein ◽  
Salt And Drought Stress ◽  
Stress Related Genes ◽  
Nac Family

The NAC (NAM, ATAF1/2, and CUC2) transcription factors comprise one of the largest transcription factor families in plants and play important roles in stress responses. However, little is known about the functions of potato NAC family members. Here we report the cloning of a potato NAC transcription factor gene StNAC053, which was significantly upregulated after salt, drought, and abscisic acid treatments. Furthermore, the StNAC053-GFP fusion protein was found to be located in the nucleus and had a C-terminal transactivation domain, implying that StNAC053 may function as a transcriptional activator in potato. Notably, Arabidopsis plants overexpressing StNAC053 displayed lower seed germination rates compared to wild-type under exogenous ABA treatment. In addition, the StNAC053 overexpression Arabidopsis lines displayed significantly increased tolerance to salt and drought stress treatments. Moreover, the StNAC053-OE lines were found to have higher activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) under multiple stress treatments. Interestingly, the expression levels of several stress-related genes including COR15A,DREB1A, ERD11, RAB18, ERF5, and KAT2, were significantly upregulated in these StNAC053-overexpressing lines. Taken together, overexpression of the stress-inducible StNAC053 gene could enhance the tolerances to both salt and drought stress treatments in Arabidopsis, likely by upregulating stress-related genes.

scholarly journals The Maize Clade A PP2C Phosphatases Play Critical Roles in Multiple Abiotic Stress Responses

2019 ◽  
Vol 20 (14) ◽  
pp. 3573 ◽  
Author(s):  
Zhenghua He ◽  
Jinfeng Wu ◽  
Xiaopeng Sun ◽  
Mingqiu Dai
Keyword(s):  
Drought Tolerance ◽  
Stress Responses ◽  
Transgenic Arabidopsis ◽  
Expression Patterns ◽  
Survival Rates ◽  
Wild Type ◽  
Multiple Stresses ◽  
Core Components ◽  
Leaf Water Loss ◽  
Natural Variations

As the core components of abscisic acid (ABA) signal pathway, Clade A PP2C (PP2C-A) phosphatases in ABA-dependent stress responses have been well studied in Arabidopsis. However, the roles and natural variations of maize PP2C-A in stress responses remain largely unknown. In this study, we investigated the expression patterns of ZmPP2C-As treated with multiple stresses and generated transgenic Arabidopsis plants overexpressing most of the ZmPP2C-A genes. The results showed that the expression of most ZmPP2C-As were dramatically induced by multiple stresses (drought, salt, and ABA), indicating that these genes may have important roles in response to these stresses. Compared with wild-type plants, ZmPP2C-A1, ZmPP2C-A2, and ZmPP2C-A6 overexpression plants had higher germination rates after ABA and NaCl treatments. ZmPP2C-A2 and ZmPP2C-A6 negatively regulated drought responses as the plants overexpressing these genes had lower survival rates, higher leaf water loss rates, and lower proline accumulation compared to wild type plants. The natural variations of ZmPP2C-As associated with drought tolerance were also analyzed and favorable alleles were detected. We widely studied the roles of ZmPP2C-A genes in stress responses and the natural variations detected in these genes have the potential to be used as molecular markers in genetic improvement of maize drought tolerance.

scholarly journals Overexpression of TaCOMT Improves Melatonin Production and Enhances Drought Tolerance in Transgenic Arabidopsis

2019 ◽  
Vol 20 (3) ◽  
pp. 652 ◽  
Author(s):  
Wen-Jing Yang ◽  
Yong-Tao Du ◽  
Yong-Bin Zhou ◽  
Jun Chen ◽  
Zhao-Shi Xu ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Triticum Aestivum L ◽  
Regulation Mechanism ◽  
Wild Type ◽  
Drought Treatment ◽  
Agricultural Applications ◽  
Transgenic Lines ◽  
Mda Content ◽  
Melatonin Production

Melatonin (N-acetyl-5-methoxytryptamine) is involved in many developmental processes and responses to various abiotic stresses in plants. Most of the studies on melatonin focus on its functions and physiological responses in plants, while its regulation mechanism remains unknown. Caffeic acid 3-O-methyltransferase (COMT) functions at a key step of the biosynthesis process of melatonin. In this study, a COMT-like gene, TaCOMT (Traes_1AL_D9035D5E0.1) was identified in common wheat (Triticum aestivum L.). Transient transformation in wheat protoplasts determined that TaCOMT is localized in cytoplasm. TaCOMT in wheat was induced by drought stress, gibberellin (GA)3 and 3-Indoleacetic acid (IAA), but not by ABA. In TaCOMT transgenic Arabidopsis, melatonin contents were higher than that in wild type (WT) plants. Under D-Mannitol treatment, the fresh weight of the transgenic Arabidopsis was significantly higher than WT, and transgenic lines had a stronger root system compared to WT. Drought tolerance assays in pots showed that the survival rate of TaCOMT-overexpression lines was significantly higher than that of WT lines. this phenotype was similar to that the WT lines treated with melatonin under drought condition. In addition, the TaCOMT transgenic lines had higher proline content and lower malondialdehyde (MDA) content compared to WT lines after drought treatment. These results indicated that overexpression of the wheat TaCOMT gene enhances drought tolerance and increases the content of melatonin in transgenic Arabidopsis. It could be one of the potential genes for agricultural applications.

scholarly journals Transcription Factor AtMYB44 Regulates Induced Expression of the ETHYLENE INSENSITIVE2 Gene in Arabidopsis Responding to a Harpin Protein

2011 ◽  
Vol 24 (3) ◽  
pp. 377-389 ◽  
Author(s):  
Ruoxue Liu ◽  
Lei Chen ◽  
Zhenhua Jia ◽  
Beibei Lü ◽  
Haojie Shi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transgenic Arabidopsis ◽  
Defense Responses ◽  
Binding Activity ◽  
Ethylene Signaling ◽  
Wild Type ◽  
Induced Expression ◽  
Harpin Protein ◽  
Ethylene Signaling Pathway ◽  
Bacterial Plant Pathogen

AtMYB44 is a transcription factor that functions in association with the ethylene-signaling pathway in Arabidopsis thaliana. The pathway depends on ETHYLENE INSENSITIVE2 (EIN2), an essential component of ethylene signaling, to regulate defense responses in the plant following treatment with HrpNEa, a harpin protein from a bacterial plant pathogen. Here, we show that AtMYB44 regulates induced expression of the EIN2 gene in HrpNEa-treated Arabidopsis plants. A HrpNEa and ethylene–responsive fragment of the AtMYB44 promoter is sufficient to support coordinate expression of AtMYB44 and EIN2 in specific transgenic Arabidopsis. In the plant, the AtMYB44 protein localizes to nuclei and binds the EIN2 promoter; the HrpNEa treatment promotes AtMYB44 production, binding activity, and transcription of AtMYB44 and EIN2. AtMYB44 overexpression results in increased production of the AtMYB44 protein and the occurrence of AtMYB44-EIN2 interaction under all genetic backgrounds of wild-type Arabidopsis and the etr1-1, ein2-1, ein3-1, and ein5-1 mutants, which have defects in the ethylene receptor ETR1 and the signal regulators EIN2, EIN3, and EIN5. However, AtMYB44 overexpression leads to enhanced EIN2 expression only under backgrounds of wild type, ein3-1, and ein5-1 but not etr1-1 and ein2-1, suggesting that ethylene perception is necessary to the regulation of EIN2 transcription by AtMYB44.

scholarly journals GmNAC5, a NAC Transcription Factor, Is a Transient Response Regulator Induced by Abiotic Stress in Soybean

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Hangxia Jin ◽  
Guangli Xu ◽  
Qingchang Meng ◽  
Fang Huang ◽  
Deyue Yu
Keyword(s):  
Transcription Factors ◽  
Abiotic Stress ◽  
Stress Responses ◽  
Apical Meristem ◽  
High Salinity ◽  
Response Regulator ◽  
Mechanical Wounding ◽  
Nac Transcription Factors ◽  
Immature Seeds ◽  
Hormone Signalling

GmNAC5 is a member of NAM subfamily belonging to NAC transcription factors in soybean (Glycine max(L.) Merr.). Studies on NAC transcription factors have shown that this family functioned in the regulation of shoot apical meristem (SAM), hormone signalling, and stress responses. In this study, we examined the expression levels ofGmNAC5.GmNAC5was highly expressed in the roots and immature seeds, especially strongly in immature seeds of 40 days after flowering. In addition, we found thatGmNAC5was induced by mechanical wounding, high salinity, and cold treatments but was not induced by abscisic acid (ABA). The subcellular localization assay suggested that GmNAC5 was targeted at nucleus. Together, it was suggested that GmNAC5 might be involved in seed development and abiotic stress responses in soybean.

scholarly journals Overexpression of Cassava MeAnn2 Enhances the Salt and IAA Tolerance of Transgenic Arabidopsis

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 941
Author(s):  
Xuejun Lin ◽  
Ruimei Li ◽  
Yangjiao Zhou ◽  
Fenlian Tang ◽  
Yajie Wang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Stress Responses ◽  
Transgenic Arabidopsis ◽  
Salt Bridge ◽  
Auxin Signaling ◽  
Storage Roots ◽  
Calcium Dependent ◽  
Auxin Signaling Pathway ◽  
Mda Content ◽  
Regulatory Effects

Annexins are a superfamily of soluble calcium-dependent phospholipid-binding proteins that have considerable regulatory effects in plants, especially in response to adversity and stress. The Arabidopsis thaliana AtAnn1 gene has been reported to play a significant role in various abiotic stress responses. In our study, the cDNA of an annexin gene highly similar to AtAnn1 was isolated from the cassava genome and named MeAnn2. It contains domains specific to annexins, including four annexin repeat sequences (I–IV), a Ca2+-binding sequence, Ca2+-independent membrane-binding-related tryptophan residues, and a salt bridge-related domain. MeAnn2 is localized in the cell membrane and cytoplasm, and it was found to be preferentially expressed in the storage roots of cassava. The overexpression of MeAnn2 reduced the sensitivity of transgenic Arabidopsis to various Ca2+, NaCl, and indole-3-acetic acid (IAA) concentrations. The expression of the stress resistance-related gene AtRD29B and auxin signaling pathway-related genes AtIAA4 and AtLBD18 in transgenic Arabidopsis was significantly increased under salt stress, while the Malondialdehyde (MDA) content was significantly lower than that of the control. These results indicate that the MeAnn2 gene may increase the salt tolerance of transgenic Arabidopsis via the IAA signaling pathway.

scholarly journals The Basic Leucine Zipper Transcription Factor Moatf1 Mediates Oxidative Stress Responses and Is Necessary for Full Virulence of the Rice Blast Fungus Magnaporthe oryzae

2010 ◽  
Vol 23 (8) ◽  
pp. 1053-1068 ◽  
Author(s):  
Min Guo ◽  
Wang Guo ◽  
Yue Chen ◽  
Suomeng Dong ◽  
Xing Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Magnaporthe Oryzae ◽  
Stress Responses ◽  
Rice Blast ◽  
Leucine Zipper ◽  
Blast Disease ◽  
Bzip Transcription Factor ◽  
Wild Type ◽  
Basic Leucine Zipper

Magnaporthe oryzae is the causal agent of rice blast disease, leading to enormous losses of rice production. Here, we characterized a basic leucine zipper (bZIP) transcription factor, Moatf1, in M. oryzae, a homolog of Schizosaccharomyces pombe ATF/CREB that regulates the oxidative stress response. Moatf1 deletion caused retarded vegetative growth of mycelia, and the Moatf1 mutant exhibited higher sensitivity to hydrogen peroxide (H2O2) than did the wild-type strain. The mutant showed severely reduced activity of extracellular enzymes and transcription level of laccases and peroxidases and exhibited significantly reduced virulence on rice cultivar CO-39. On rice leaf sheath, most of the infectious hyphae of the mutant became swollen and displayed restricted growth in primary infected cells. Defense response was strongly activated in plants infected by the mutant. Diamino benzidine staining revealed an accumulation of H2O2 around Moatf1 mutant appressoria and rice cells with Moatf1 hyphae that was absent in the wild type. Inhibition of the plant NADPH oxidase by diphenyleneiodonium prevented host-derived H2O2 accumulation and restored infectious hyphal growth of the mutant in rice cells. Thus, we conclude that Moatf1 is necessary for full virulence of M. oryzae by regulating the transcription of laccases and peroxidases to impair reactive oxygen species–mediated plant defense.

scholarly journals GmNAC06, a NAC domain transcription factor enhances salt stress tolerance in soybean

2020 ◽  
Author(s):  
Ming Li ◽  
Rui Chen ◽  
Qiyan Jiang ◽  
Xianjun Sun ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Hairy Roots ◽  
Stress Responses ◽  
High Salinity ◽  
Negative Effects ◽  
Ionic Homeostasis ◽  
Transgenic Hairy Roots ◽  
Localization Analysis ◽  
Soybean Plants

Key message We found GmNAC06 plays an important role in salt stress responses through the phenotypic, physiological and molecular analyses of OE, VC, and Mutant composite soybean. Abstract Salinization affects 20% of all cultivated land worldwide because of the high salinity of irrigation water and the excessive use of water, and this amount is increasing daily. NAC (NAM, ATAF, and CUC) have been found to be involved in salt stress. In this study, a soybean NAC gene, GmNAC06 (Glyma06g21020.1), was cloned and functionally characterized. The results of expression analysis suggested that salt stress could influence the expression level of GmNAC06. The subcellular localization analysis results suggested that GmNAC06 may function as a transcription factor. Under salt stress, the overexpression technology combined with CRISPR-Cas9 system found that GmNAC06 could cause the accumulation of proline and glycine betaine to alleviate or avoid the negative effects of ROS; similarly, it could control the Na+/K+ ratios in hairy roots to maintain ionic homeostasis. The fresh weight of the transgenic hairy roots and the histochemical ROS staining of wild leaves suggested that transgenic hairy roots influence the function of wild leaves under salt stress conditions. Moreover, the expression levels of GmUBC2 and GmHKT1 were higher in the GmNAC06 hairy roots than in the control. Thus, the overexpression of GmNAC06 in hairy roots notably causes an entire composite plant to exhibit salt tolerance. The phenotype of composite soybean plants and transgenic Arabidopsis plants suggest that GmNAC06 plays a role in response to salt stress and could be useful in generating salt tolerant transgenic crops.

scholarly journals Ectopic Expression of Gs5PTase8, a Soybean Inositol Polyphosphate 5-Phosphatase, Enhances Salt Tolerance in Plants

2020 ◽  
Vol 21 (3) ◽  
pp. 1023 ◽  
Author(s):  
Qi Jia ◽  
Song Sun ◽  
Defeng Kong ◽  
Junliang Song ◽  
Lumei Wu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Transgenic Arabidopsis ◽  
Glycine Soja ◽  
Critical Role ◽  
Ectopic Expression ◽  
Wild Soybean ◽  
Inositol Polyphosphate ◽  
Wild Type

Inositol polyphosphate 5-phosphatases (5PTases) function in inositol signaling by regulating the catabolism of phosphoinositol derivatives. Previous reports showed that 5PTases play a critical role in plant development and stress responses. In this study, we identified a novel 5PTase gene, Gs5PTase8, from the salt-tolerance locus of chromosome 3 in wild soybean (Glycine soja). Gs5PTase8 is highly up-regulated under salt treatment. It is localized in the nucleus and plasma membrane with a strong signal in the apoplast. Ectopic expression of Gs5PTase8 significantly increased salt tolerance in transgenic BY-2 cells, soybean hairy roots and Arabidopsis, suggesting Gs5PTase8 could increase salt tolerance in plants. The overexpression of Gs5PTase8 significantly enhanced the activities of catalase and ascorbate peroxidase under salt stress. The seeds of Gs5PTase8-transgenic Arabidopsis germinated earlier than the wild type under abscisic acid treatment, indicating Gs5PTase8 would alter ABA sensitivity. Besides, transcriptional analyses showed that the stress-responsive genes, AtRD22, AtRD29A and AtRD29B, were induced with a higher level in the Gs5PTase8-transgenic Arabidopsis plants than in the wild type under salt stress. These results reveal that Gs5PTase8 play a positive role in salt tolerance and might be a candidate gene for improving soybean adaptation to salt stress.

scholarly journals The Arabidopsis transcription factor ABIG1 relays ABA signaled growth inhibition and drought induced senescence

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Tie Liu ◽  
Adam D Longhurst ◽  
Franklin Talavera-Rauh ◽  
Samuel A Hokin ◽  
M Kathryn Barton
Keyword(s):  
Transcription Factor ◽  
Growth Inhibition ◽  
Stress Responses ◽  
Stomatal Closure ◽  
Mrna Levels ◽  
Wild Type ◽  
Drought Resistant ◽  
Aba Insensitive ◽  
Senesced Leaves ◽  
New Strategies

Drought inhibits plant growth and can also induce premature senescence. Here we identify a transcription factor, ABA INSENSITIVE GROWTH 1 (ABIG1) required for abscisic acid (ABA) mediated growth inhibition, but not for stomatal closure. ABIG1 mRNA levels are increased both in response to drought and in response to ABA treatment. When treated with ABA, abig1 mutants remain greener and produce more leaves than comparable wild-type plants. When challenged with drought, abig1 mutants have fewer yellow, senesced leaves than wild-type. Induction of ABIG1 transcription mimics ABA treatment and regulates a set of genes implicated in stress responses. We propose a model in which drought acts through ABA to increase ABIG1 transcription which in turn restricts new shoot growth and promotes leaf senescence. The results have implications for plant breeding: the existence of a mutant that is both ABA resistant and drought resistant points to new strategies for isolating drought resistant genetic varieties.

Sign in / Sign up

Export Citation Format

Share Document