scholarly journals The Roles of Auxin Biosynthesis YUCCA Gene Family in Plants

2019 ◽  
Vol 20 (24) ◽  
pp. 6343 ◽  
Author(s):  
Xu Cao ◽  
Honglei Yang ◽  
Chunqiong Shang ◽  
Sang Ma ◽  
Li Liu ◽  
...  
Keyword(s):  
Gene Family ◽  
Plant Development ◽  
Normal Growth ◽  
Molecular Structures ◽  
Auxin Biosynthesis ◽  
Auxin Signaling ◽  
Oxidative Decarboxylation ◽  
Auxin Signaling Pathway ◽  
Dynamic Expression ◽  
Environmental Responses

Auxin plays essential roles in plant normal growth and development. The auxin signaling pathway relies on the auxin gradient within tissues and cells, which is facilitated by both local auxin biosynthesis and polar auxin transport (PAT). The TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA)/YUCCA (YUC) pathway is the most important and well-characterized pathway that plants deploy to produce auxin. YUCs function as flavin-containing monooxygenases (FMO) catalyzing the rate-limiting irreversible oxidative decarboxylation of indole-3-pyruvate acid (IPyA) to form indole-3-acetic acid (IAA). The spatiotemporal dynamic expression of different YUC gene members finely tunes the local auxin biosynthesis in plants, which contributes to plant development as well as environmental responses. In this review, the recent advances in the identification, evolution, molecular structures, and functions in plant development and stress response regarding the YUC gene family are addressed.

scholarly journals Transcriptional activation of auxin biosynthesis drives developmental reprogramming of differentiated cells

2021 ◽  
Author(s):  
Yuki Sakamoto ◽  
Ayako Kawamura ◽  
Takamasa Suzuki ◽  
Shoji Segami ◽  
Masayoshi Maeshima ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Fate ◽  
Transcriptional Activation ◽  
Callus Formation ◽  
Mechanistic Model ◽  
Plant Cells ◽  
Auxin Biosynthesis ◽  
Auxin Signaling ◽  
Differentiated Cells ◽  
Auxin Signaling Pathway

Plant cells exhibit remarkable plasticity of their differentiation states, enabling regeneration of whole plants from differentiated somatic cells. How they revert cell fate and express pluripotency, however, remains unclear. Here we show that transcriptional activation of auxin biosynthesis is crucial for reprogramming differentiated Arabidopsis leaf cells. We demonstrate that interfering with the activity of histone acetyltransferases dramatically reduces callus formation from leaf mesophyll protoplasts. Impaired histone acetylation predominantly affects transcription of auxin biosynthesis genes. Auxin biosynthesis is in turn required to accomplish initial cell division through the activation of G2/M phase genes mediated by MYB DOMAIN PROTEIN 3-RELATED (MYB3Rs). We further show that the AUXIN RESPONSE FACTOR 7 (ARF7)/ARF19 and INDOLE-3-ACETIC ACID INDUCIBLE 3 (IAA3)/IAA18-mediated auxin signaling pathway is responsible for cell cycle reactivation in protoplasts. These findings provide novel mechanistic model of how differentiated plant cells can revert their fate and reinitiate the cell cycle to become pluripotent.

scholarly journals Membrane Sterol Composition in Arabidopsis thaliana Affects Root Elongation via Auxin Biosynthesis

2021 ◽  
Vol 22 (1) ◽  
pp. 437
Author(s):  
Meng Wang ◽  
Panpan Li ◽  
Yao Ma ◽  
Xiang Nie ◽  
Markus Grebe ◽  
...  
Keyword(s):  
Root Elongation ◽  
Auxin Transport ◽  
Sterol Composition ◽  
Polar Auxin Transport ◽  
Sterol Biosynthesis ◽  
Auxin Biosynthesis ◽  
Root Tip ◽  
Auxin Signaling ◽  
Auxin Response ◽  
Short Root

Plant membrane sterol composition has been reported to affect growth and gravitropism via polar auxin transport and auxin signaling. However, as to whether sterols influence auxin biosynthesis has received little attention. Here, by using the sterol biosynthesis mutant cyclopropylsterol isomerase1-1 (cpi1-1) and sterol application, we reveal that cycloeucalenol, a CPI1 substrate, and sitosterol, an end-product of sterol biosynthesis, antagonistically affect auxin biosynthesis. The short root phenotype of cpi1-1 was associated with a markedly enhanced auxin response in the root tip. Both were neither suppressed by mutations in polar auxin transport (PAT) proteins nor by treatment with a PAT inhibitor and responded to an auxin signaling inhibitor. However, expression of several auxin biosynthesis genes TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS1 (TAA1) was upregulated in cpi1-1. Functionally, TAA1 mutation reduced the auxin response in cpi1-1 and partially rescued its short root phenotype. In support of this genetic evidence, application of cycloeucalenol upregulated expression of the auxin responsive reporter DR5:GUS (β-glucuronidase) and of several auxin biosynthesis genes, while sitosterol repressed their expression. Hence, our combined genetic, pharmacological, and sterol application studies reveal a hitherto unexplored sterol-dependent modulation of auxin biosynthesis during Arabidopsis root elongation.

scholarly journals TAA1-Mediated Auxin Biosynthesis Is Essential for Hormone Crosstalk and Plant Development

Cell ◽  
2008 ◽  
Vol 133 (1) ◽  
pp. 177-191 ◽  
Author(s):  
Anna N. Stepanova ◽  
Joyce Robertson-Hoyt ◽  
Jeonga Yun ◽  
Larissa M. Benavente ◽  
De-Yu Xie ◽  
...  
Keyword(s):  
Plant Development ◽  
Auxin Biosynthesis ◽  
Hormone Crosstalk

scholarly journals Auxin requirements for a meristematic state in roots depend on a dual brassinosteroid function

2020 ◽  
Author(s):  
M. Ackerman-Lavert ◽  
Y. Fridman ◽  
R Matosevich ◽  
H Khandal ◽  
L. Friedlander ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Auxin Biosynthesis ◽  
Auxin Signaling ◽  
Critical Ratio ◽  
Dual Effect ◽  
Normal Morphology ◽  
Arabidopsis Root ◽  
Auxin Synthesis ◽  
Meristem Maintenance ◽  
Transcriptional Output

SummaryThe organization of the root meristem is maintained by a complex interplay between plant hormones signaling pathways that both interpret and determine their accumulation and distribution. Brassinosteroids (BR) and auxin signaling pathways control the number of meristematic cells in the Arabidopsis root, via an interaction that appears to involve contradicting molecular outcomes, with BR promoting auxin signaling input but also repressing its output. However, whether this seemingly incoherent effect is significant for meristem function is unclear. Here, we established that a dual effect of BR on auxin, with BR simultaneously promoting auxin biosynthesis and repressing auxin transcriptional output, is essential for meristem maintenance. Blocking BR-induced auxin synthesis resulted in rapid BR-mediated meristem loss. Conversely, plants with reduced BR levels were resistant to loss of auxin biosynthesis and these meristems maintained their normal morphology despite a 10-fold decrease in auxin levels. In agreement, injured root meristems which rely solely on local auxin synthesis, regenerated when both auxin and BR synthesis were inhibited. Use of BIN2 as a tool to selectively inhibit BR signaling, revealed meristems with distinct phenotypes depending on the perturbed tissue; meristem reminiscent of BR-deficient mutants or of high BR exposure. This enabled mapping BR-auxin interactions to the outer epidermis and lateral root cap tissues, and demonstrated the essentiality of BR signaling in these tissues for meristem maintenance. BR activity in internal tissues however, proved necessary to control BR homeostasis. Together, we demonstrate a basis for inter-tissue coordination and how a critical ratio between these hormones determines the meristematic state.

scholarly journals A chemically induced proximity system engineered from the plant auxin signaling pathway

2018 ◽  
Vol 9 (26) ◽  
pp. 5822-5827 ◽  
Author(s):  
Weiye Zhao ◽  
Huong Nguyen ◽  
Guihua Zeng ◽  
Dan Gao ◽  
Hao Yan ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Auxin Signaling ◽  
Auxin Signaling Pathway ◽  
Chemically Induced

A new chemically induced proximity system is developed by engineering the plant auxin signaling pathway.

Identification of Auxin Activity Like 1, a chemical with weak functions in auxin signaling pathway

2018 ◽  
Vol 98 (3) ◽  
pp. 275-287
Author(s):  
Wenbo Li ◽  
Haimin Li ◽  
Peng Xu ◽  
Zhi Xie ◽  
Yajin Ye ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Auxin Signaling ◽  
Auxin Activity ◽  
Auxin Signaling Pathway

scholarly journals Molecular Rewiring of the Jasmonate Signaling Pathway to Control Auxin-Responsive Gene Expression

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 641
Author(s):  
Ning Li ◽  
Linggai Cao ◽  
Wenzhuo Miu ◽  
Ruibin Cao ◽  
Mingbo Peng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signaling Pathway ◽  
Developmental Process ◽  
Expression System ◽  
Transient Gene Expression ◽  
Transcriptional Repressor ◽  
Auxin Signaling ◽  
General Strategy ◽  
Auxin Signaling Pathway ◽  
Responsive Gene

The plant hormone jasmonic acid (JA) has an important role in many aspects of plant defense response and developmental process. JA triggers interaction between the F-box protein COI1 and the transcriptional repressors of the JAZ family that leads the later to proteasomal degradation. The Jas-motif of JAZs is critical for mediating the COI1 and JAZs interaction in the presence of JA. Here, by using the protoplast transient gene expression system we reported that the Jas-motif of JAZ1 was necessary and sufficient to target a foreign reporter protein for COI1-facilitated degradation. We fused the Jas-motif to the SHY2 transcriptional repressor of auxin signaling pathway to create a chimeric protein JaSHY. Interestingly, JaSHY retained the transcriptional repressor function while become degradable by the JA coreceptor COI1 in a JA-dependent fashion. Moreover, the JA-induced and COI1-facilitated degradation of JaSHY led to activation of a synthetic auxin-responsive promoter activity. These results showed that the modular components of JA signal transduction pathway can be artificially redirected to regulate auxin signaling pathway and control auxin-responsive gene expression. Our work provides a general strategy for using synthetic biology approaches to explore and design cell signaling networks to generate new cellular functions in plant systems.

Environmental responses of the FT/TFL1 gene family and their involvement in flower induction in Fragaria × ananassa

2015 ◽  
Vol 177 ◽  
pp. 60-66 ◽  
Author(s):  
Yoshihiro Nakano ◽  
Yohei Higuchi ◽  
Yuichi Yoshida ◽  
Tamotsu Hisamatsu
Keyword(s):  
Gene Family ◽  
Flower Induction ◽  
Fragaria Ananassa ◽  
Environmental Responses
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