scholarly journals Laser-Driven Ultrashort Pulsed Electron Beam Radiation at Doses of 0.5 and 1.0 Gy Induces Apoptosis in Human Fibroblasts

2019 ◽  
Vol 20 (20) ◽  
pp. 5140 ◽  
Author(s):  
Nelly Babayan ◽  
Bagrat Grigoryan ◽  
Lusine Khondkaryan ◽  
Gohar Tadevosyan ◽  
Natalya Sarkisyan ◽  
...  
Keyword(s):  
Cell Death ◽  
Electron Beam ◽  
Human Fibroblasts ◽  
Lung Fibroblasts ◽  
Particle Accelerators ◽  
X Rays ◽  
Beam Radiation ◽  
X Ray ◽  
Pulsed Electron Beam ◽  
Γh2ax Foci

Rapidly evolving laser technologies have led to the development of laser-generated particle accelerators as an alternative to conventional facilities. However, the radiobiological characteristics need to be determined to enhance their applications in biology and medicine. In this study, the radiobiological effects of ultrashort pulsed electron beam (UPEB) and X-ray radiation in human lung fibroblasts (MRC-5 cell line) exposed to doses of 0.1, 0.5, and 1 Gy are compared. The changes of γH2AX foci number as a marker of DNA double-strand breaks (DSBs) were analyzed. In addition, the micronuclei induction and cell death via apoptosis were studied. We found that the biological action of UPEB-radiation compared to X-rays was characterized by significantly slower γH2AX foci elimination (with a dose of 1 Gy) and strong apoptosis induction (with doses of 0.5 and 1.0 Gy), accompanied by a slight increase in micronuclei formation (dose of 1 Gy). Our data suggest that UPEB radiation produces more complex DNA damage than X-ray radiation, leading to cell death rather than cytogenetic disturbance.

X-ray micro tomography in the scanning electron microscope

1978 ◽  
Vol 36 (1) ◽  
pp. 106-107 ◽  
Author(s):  
W. Brünger
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Target Surface ◽  
The Body ◽  
X Rays ◽  
Cross Sectional ◽  
X Ray ◽  
Micro Tomography ◽  
Scanning Electron

Reconstructive tomography is a new technique in diagnostic radiology for imaging cross-sectional planes of the human body /1/. A collimated beam of X-rays is scanned through a thin slice of the body and the transmitted intensity is recorded by a detector giving a linear shadow graph or projection (see fig. 1). Many of these projections at different angles are used to reconstruct the body-layer, usually with the aid of a computer. The picture element size of present tomographic scanners is approximately 1.1 mm2.Micro tomography can be realized using the very fine X-ray source generated by the focused electron beam of a scanning electron microscope (see fig. 2). The translation of the X-ray source is done by a line scan of the electron beam on a polished target surface /2/. Projections at different angles are produced by rotating the object.During the registration of a single scan the electron beam is deflected in one direction only, while both deflections are operating in the display tube.

scholarly journals Exposure of the cytoplasm to low-dose X-rays modifies ataxia telangiectasia mutated-mediated DNA damage responses

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Munetoshi Maeda ◽  
Masanori Tomita ◽  
Mika Maeda ◽  
Hideki Matsumoto ◽  
Noriko Usami ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Death ◽  
Ataxia Telangiectasia ◽  
Kinase Inhibitor ◽  
Surrogate Marker ◽  
X Rays ◽  
Ataxia Telangiectasia Mutated ◽  
Whole Cell ◽  
X Ray ◽  
Dna Damage Responses

AbstractWe recently showed that when a low X-ray dose is used, cell death is enhanced in nucleus-irradiated compared with whole-cell-irradiated cells; however, the role of the cytoplasm remains unclear. Here, we show changes in the DNA damage responses with or without X-ray microbeam irradiation of the cytoplasm. Phosphorylated histone H2AX foci, a surrogate marker for DNA double-strand breaks, in V79 and WI-38 cells are not observed in nucleus irradiations at ≤ 2 Gy, whereas they are observed in whole-cell irradiations. Addition of an ataxia telangiectasia mutated (ATM) kinase inhibitor to whole-cell irradiations suppresses foci formation at ≤ 2 Gy. ABL1 and p73 expression is upregulated following nucleus irradiation, suggesting the induction of p73-dependent cell death. Furthermore, CDKN1A (p21) is upregulated following whole-cell irradiation, indicating the induction of cell cycle arrest. These data reveal that cytoplasmic radioresponses modify ATM-mediated DNA damage responses and determine the fate of cells irradiated at low doses.

Deposition of Cobalt Doped Zinc Oxide Thin Film Nano-Composites Via Pulsed Electron Beam Ablation

MRS Advances ◽  
2016 ◽  
Vol 1 (6) ◽  
pp. 433-439 ◽  
Author(s):  
Asghar Ali ◽  
Patrick Morrow ◽  
Redhouane Henda ◽  
Ragnar Fagerberg
Keyword(s):  
Zinc Oxide ◽  
Electron Beam ◽  
Binding Energy ◽  
Photoelectron Spectroscopy ◽  
Xrd Analysis ◽  
Sem Analysis ◽  
Oxide Thin Film ◽  
X Ray ◽  
Pulsed Electron Beam ◽  
Deposited Films

AbstractThis study reports on the preparation of cobalt doped zinc oxide (Co:ZnO) films via pulsed electron beam ablation (PEBA) from a single target containing 20 w% Co on sapphire (0001) and silicon (100) substrates. The films have been deposited at various temperatures (350оC, 400оC, 450оC) and pulse frequencies (2 Hz, 4 Hz), under a background argon (Ar) pressure of about 3 mtorr, and an accelerating voltage of 14 kV. The surface morphology has been examined by atomic force microscopy (AFM) and scanning electron microscopy (SEM). According to SEM analysis, the films consist of nano-globules whose size is in the range of 80-178 nm. Energy dispersive x-ray spectroscopy (EDX) reveals that deposition is congruent and the prepared films contain ∼20±5 w% cobalt. It has been found that the nano-globules in the deposited films are cobalt-rich zones containing ∼70 w% Co. From x-ray photoelectron spectroscopy (XPS) analysis, Co 2p3/2 peaks indicate that the deposited films contain CoO (binding energy = 780.5 eV) as well as metallic Co (binding energy = 778.1-778.5 eV). X-ray diffraction (XRD) analysis supports the presence of metallic Co hcp phase (2ϴ = 44.47° and 47.43°) in the films.

Influence of Electron Beam Pulsed Times on the Properties of 40Cr

2010 ◽  
Vol 154-155 ◽  
pp. 1170-1177
Author(s):  
Yuan Fang Chen ◽  
Xiao Dong Peng ◽  
Jian Jun Hu ◽  
Hong Bin Xu ◽  
Chan Hao
Keyword(s):  
Surface Roughness ◽  
Wear Resistance ◽  
Corrosion Resistance ◽  
Electron Beam ◽  
High Current ◽  
Wear Properties ◽  
X Ray ◽  
Pulsed Electron Beam ◽  
Surface Microstructures ◽  
40Cr Steel

Surface modification of 40Cr steel by high current pulsed electron beam has been investigated . The pulsed times of HCPEB was changed from 1 to 25 to prepare different specimens. Surface microstructures and section microstructures after HCPEB irradiation were detected by using metallurgical microscope, SEM and X-ray diffractometer. It is shown that crater defects were found on the surface after the irradiation of HCPEB and the density of craters will decrease with increasing pulses times. When treated by 27Kev accelerating voltage, with increasing pulse times, the particles located in surface layer were obviously refined .The surface roughness, hardness, wear properties and corrosion resistance were analyzed after irradiation of HCPEB. The wear resistance and corrosion resistance were obviously enhanced after 10 pulses treatment.

Some of these could also be operated in the energy range above lOMeV for experiments designed to determine at which energy level radioactivity can be induced in the irradiated medium. A linac with a maximum energy of 25 MeV was commissioned for the U.S. Army Natick Research and Development Labora­ tories in 1963. Its beam power was 6.5 kW at an electron energy of 10 MeV, 18 kW at 24 MeV. Assuming 100% efficiency, a 1-kW beam can irradiate 360 kg of product with a dose of 10 kGy/h. The efficiency of electron accelerators is higher than that of gamma sources because the electron beam can be directed at the product, whereas the gamma sources emit radiation in all directions. An efficiency of 50% is a realistic assumption for accelerator facilities. With that and 6.5 kW beam power an accelerator of the type built for the Natick laboratories can process about 1.2t/h at 10 kGy. In Odessa in the former Soviet Union, now in the Ukraine, two 20-kW accelerators with an energy of 1.4 MeV installed next to a grain elevator went into operation in 1983. Each accelerator has the capacity to irradiate 200 t of wheat per hour with a dose of 200 Gy for insect disinfestation. This corresponds to a beam utilization of 56% (9). In France, a facility for electron irradiation of frozen deboned chicken meat commenced operation at Berric near Vannes (Brittany) in late 1986. The purpose of irradiation is to improve the hygienic quality of the meat by destroying salmonella and other disease-causing (pathogenic) microorganisms. The electron beam accelerator is a 7 MeV/10 kW Cassitron built by CGR-MeV (10). An irradiation facility of this type is shown in Figure . Because of their relatively low depth of penetration electron beams cannot be used for the irradiation of animal carcasses, large packages, or other thick materials. However, this difficulty can be overcome by converting the electrons to x-rays. As indicated in Figure 9, this can be done by fitting a water-cooled metal plate to the scanner. Whereas in conventional x-ray tubes the conversion of electron energy to x-ray energy occurs only with an efficiency of about %, much higher efficiencies can be achieved in electron accelerators. The conversion efficiency depends on the material of the converter plate (target) and on the electron energy. Copper converts 5-MeV electrons with about 7% efficiency, 10-MeV electrons with 12% efficiency. A tungsten target can convert 5-MeV electrons with about 20%, 10-MeV electrons with 30% efficiency. (Exact values depend on target thickness.) In contrast to the distinct gamma radiation energy emitted from radionuclides and to the monoenergetic electrons produced by accelerators, the energy spectrum of x-rays is continuous from the value equivalent to the energy of the bombarding electrons to zero. The intensity of this spectrum peaks at about one-tenth of the maximum energy value. The exact location of the intensity peak depends on the thickness of the converter plate and on some other factors. As indicated in Figure

1995 ◽  
pp. 40-40
Keyword(s):  
Electron Beam ◽  
Electron Energy ◽  
Maximum Energy ◽  
Metal Plate ◽  
Beam Power ◽  
X Rays ◽  
X Ray ◽  
Emit Radiation ◽  
Electron Accelerators ◽  
Gamma Sources

PRODUCTION OF FEMTOSECOND PULSES AND MICRON BEAM SPOTS FOR HIGH BRIGHTNESS ELECTRON BEAM APPLICATIONS

2007 ◽  
Vol 22 (22) ◽  
pp. 3726-3735
Author(s):  
S. G. ANDERSON ◽  
D. J. GIBSON ◽  
F. V. HARTEMANN ◽  
J. S. JACOB ◽  
A. M. TREMAINE ◽  
...  
Keyword(s):  
Electron Beam ◽  
Electron Beams ◽  
Beam Radiation ◽  
High Brightness ◽  
X Ray ◽  
Inverse Compton Scattering ◽  
Inverse Compton ◽  
Strong Focusing ◽  
Moderate Energy ◽  
Low Emittance

Current and future applications of high brightness electron beams, which include advanced accelerators and beam-radiation interactions require both transverse and longitudinal beam sizes on the order of tens of microns. Ultra-high density beams may be produced at moderate energy (50 MeV) by compression and subsequent strong focusing of low emittance, photoinjector sources. We describe the implementation of this method used at the PLEIADES inverse-Compton scattering (ICS) x-ray source at LLNL in which the photoinjector-generated beam has been compressed to 300 fsec rms duration using the velocity bunching technique and focused to 20 μm rms size using an extremely high gradient, permanent magnet quadrupole focusing system.

STUDY OF TRANSVERSE EFFECTS IN THE PRODUCTION OF X-RAYS WITH A FREE-ELECTRON LASER BASED ON AN OPTICAL UNDULATOR

2007 ◽  
Vol 22 (23) ◽  
pp. 4270-4279
Author(s):  
A. BACCI ◽  
C. MAROLI ◽  
V. PETRILLO ◽  
L. SERAFNI ◽  
M. FERRARIO
Keyword(s):  
Electron Beam ◽  
Free Electron ◽  
Free Electron Laser ◽  
Laser Energy ◽  
Laser Pulses ◽  
Collective Effects ◽  
Laser Source ◽  
X Rays ◽  
X Ray ◽  
Back Scattering

The interaction between high-brilliance electron beams and counter-propagating laser pulses produces X rays via Thomson back-scattering. If the laser source is long and intense enough, the electrons of the beam can bunch and a regime of collective effects can establish. In this case of dominating collective effects, the FEL instability can develop and the system behaves like a free-electron laser based on an optical undulator. Coherent X-rays can be irradiated, with a bandwidth very much thinner than that of the corresponding incoherent emission. The emittance of the electron beam and the distribution of the laser energy are the principal quantities that limit the growth of the X-ray signal. In this work we analyse with a 3-D code the transverse effects in the emission produced by a relativistic electron beam when it is under the action of an optical laser pulse and the X-ray spectra obtained. The scalings typical of the optical wiggler, characterized by very short gain lengths and overall time durations of the process make possible considerable emission also with emittance of the order of 1mm mrad.

scholarly journals X-ray Analysis in the Low Vacuum SEM (Part 3 of 3)

1998 ◽  
Vol 6 (10) ◽  
pp. 10-13
Author(s):  
Don Chernoff
Keyword(s):  
Electron Beam ◽  
Path Length ◽  
Experimental Methods ◽  
Gas Pressure ◽  
Operating Conditions ◽  
X Rays ◽  
X Ray ◽  
Software Models ◽  
Working Distance ◽  
Low Vacuum Sem

In this third and final installment on x-ray analysis in the environmental and low vacuum SEM, I will present experimental methods for measuring beam scatter. In my previous two articles I discussed how operating conditions detemine beam scatter. It was shown that the type of gas used, the gas pressure in the chamber, the working distance or beam gas path length, and the accelerating voltage all have an effect on how much the electron beam scatters. I also discussed how the beam scatter influences x-ray results by producing x-rays beyond the area of the primary beam. Furthermore, I showed how software models could be used to determine the amount of beam scatter based on different combinations of the four variables (pressure, gas, working distance, and kV).

A Universal Equation for the Emission Range of X Rays from Bulk Specimens

2007 ◽  
Vol 13 (5) ◽  
pp. 354-357 ◽  
Author(s):  
Raynald Gauvin
Keyword(s):  
Electron Beam ◽  
Free Path ◽  
Beam Energy ◽  
Mean Free Path ◽  
Electron Beam Energy ◽  
X Rays ◽  
Bulk Materials ◽  
Universal Equation ◽  
Electron Trajectories ◽  
X Ray

The derivation of a universal equation to compute the range of emitted X rays is presented for homogeneous bulk materials. This equation is based on two fundamental assumptions: the φ(ρz) curve of X-ray generation is constant and the ratio of the emitted to the generated X-ray range is equal to the ratio of the emitted to the generated X-ray intensity. An excellent agreement is observed with data obtained from Monte Carlo simulations of 200,000 electron trajectories in C, Al, Cu, Ag, Au, and an Fe–B alloy with boron weight fractions equal to 0.01, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, and 0.99, performed with the electron beam energy varied from 1 to 30 keV in 1-keV steps. When the ratio of the generated X-ray range to the photon mean free path is much smaller than one, the emission X-ray range is equal to the generated X-ray range, but when this ratio is much greater than one, the emission X-ray range is constant and is given by the product of the effective photon mean free path multiplied by the sine of the take-off angle.

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