scholarly journals Zymolytic Grain Extract (ZGE) Significantly Extends the Lifespan and Enhances the Environmental Stress Resistance of Caenorhabditis elegans

2019 ◽  
Vol 20 (14) ◽  
pp. 3489
Author(s):  
Lu Hou ◽  
Mengying Jiang ◽  
Qiong Guo ◽  
Wei Shi

Many reports have shown that grains play an important role in our daily lives and can provide energy and nutrients to protect us from various diseases, and they are considered to be indispensable parts of our lives. It has been reported that some constituents in grains could exert functional effects against HIV infections and multiple cancers. Zymolytic grain can produce some new useful molecules and thus support the cell nutrients in the human body. In this study, the effects of zymolytic grain extract (ZGE) supernatants on the changes of nematode indicators were investigated, including lifespan, self-brood size, and body length in environmental conditions (temperature, ultraviolet radiation or 5-fluoro-2′-deoxyuridine (FUDR) stimuli). We found that, compared to the control group, the ZGE supernatant-feeding group could prolong the lifespan of nematodes under normal conditions. More importantly, ZGE supernatants could improve the ability of nematodes to resist stress. When the concentration of FUDR was 400 or 50 μM, the ZGE supernatant-feeding group could prolong lifespan by an average of 38.4% compared to the control group, and the eggs of the ZGE supernatant-feeding group could hatch and develop into adults. These results indicated that ZGE could protect C. elegans from external stress and thus prolong their lifespan and improve the physiological state of nematodes. Therefore, ZGE supernatant has potential to be used as a nutritional product in antioxidant and anti-aging research.

Author(s):  
G. Uskov ◽  
A. Tsopanova ◽  
T. Perezhogina

Complete feeding of ponies is provided on the basis of data on their nutritional needs depending on age, sex, physiological state and level of productivity (the amount of milk produced and the intensity of growth of young animals). Ponies are sensitive to a lack of vitamins and mineral elements in the feed. When there is a sufficient amount of organic and mineral substances, but a lack or absence of vitamins, horses and ponies have impaired metabolism. The purpose of this work is to study the effectiveness of the use of vitamin and mineral additive MEGA-VIT in the rations of pregnant and lactating mares of Shetland pony breed. It has been found during of the researches that the vitamin and mineral additive MEGA-VIT had a positive influence on the productive and physiological indicators of animals. The cost of spent feed for the entire period of experiment in the control group was 50,6 thousand rubles, and in the experimental group it was 11,8 thousand rubles more or 23,5 %. Revenue from the sale of young horses of the control group amounted to 400 thousand rubles, and experimental group – 440 thousand rubles, this is by 40 thousand rubles more than in control group. This led to the increase in profit in the experimental group of mares by 28,1 thousand rubles and accordingly the level of profitability by 3,2 %. It has been recommended on the results have been obtained on the base of researches to include 30 g/head/day in the rations of mares of Shetland pony breed during pregnancy, and 50 g/head/day during lactation.


The article is devoted to the solution of an urgent problem- influence of different lighting modes on the dairy productivity of cows. 2 groups of cows with 20 heads each were formed. In control group, light in the cowshed was 50-75 Lux for a light period of 7.5 h in January to 16.5 h in June, and in experimental group - 150-200 Lux and 16 h, respectively. It was found that the intensity and duration of illumination affects physiological state, reproductive ability and milk productivity of cows. In the experimental group of cows, compared with the control group, hemoglobin content in blood increased by 4.6% (P < 0.01), red blood cells - by 20.6% (P < 0.05), total protein - by 11.2% (P < 0.001), glucose - by 39.1% (P < 0.05). There was a tendency to increase the total calcium and inorganic phosphorus in blood serum of cows of the experimental group. The level of alkaline phosphatase in blood serum of cows in the control group was 71.5% (P < 0.01) higher than that of cows in the experimental group. Milk yield per 1 cow in the experimental cowshed was 433 kg more than in the control. The cost of 1 kg of milk in the experimental group was 0.94 rubles lower, and the profitability of milk production and sales is 9.42% higher than in the control group. To increase the milk productivity of cows, it is recommended to increase light level in barns for tethered keeping to 150-200 Lux, with the duration of lighting in the winter and transition periods of year up to 16 hours per day.


2020 ◽  
Vol 18 (3) ◽  
pp. 194-200
Author(s):  
Maryam Moradi ◽  
Alireza Tabibzadeh ◽  
Davod Javanmard ◽  
Saied Ghorbani ◽  
Farah Bokharaei-Salim ◽  
...  

Background: Coinfection of Hepatitis C virus (HCV) with human immunodeficiency virus (HIV) has a higher risk of mortality than HCV or HIV monoinfection. HCV and HIV infections are specified by systemic inflammation, but the inflammation process in HCV/HIV coinfection is much complicated and is not well characterized. Objective: The aim of this study was to analyze the expression of TLR-3, TLR-7, IL-10, IFN-1 (IFN-α, IFN-β), and TNF-α in HIV, HCV and HIV/HCV co-infected patients. Methods: Forty-five patients including HIV group (n=15), HCV group (n=15), HIV/HCV coinfection group (n=15) and healthy control group (n=15) participated. Peripheral blood mononuclear cells (PBMCs) were obtained. PBMC-RNA, HCV and HIV RNA were extracted from all subjects and cDNA was synthesized. The viral load analyzed by reverse transcription-quantitative PCR (RT-qPCR), and the expression levels of IFN-α, IFN-β, TLR-3, TLR-7, TNF, and IL-10 mRNA were quantified in PBMCs. Results: The levels of IFN-I, IL-10, and TNF-α were overexpressed in all patients’ groups (P<0.05), TLR-7 was upregulated in all groups, but this upregulation was not statistically significant (p>0.05). TLR-3 showed a decrease in all patient groups (P<0.05). The statistical analysis demonstrated that TLR-3 has a negative correlation with HIV load, whereas other genes positively correlated with HIV load. In addition, TLR-3, TNF-α, and IFN-I were negatively correlated with HCV load, whereas TLR-7 and IL-10 s were positively correlated with HCV load. Conclusion: Our results showed a significant relationship between the expression level of innate immunity genes and inflammation in HCV, HIV, and HIV/HCV coinfected patients.


2021 ◽  
Vol 22 (2) ◽  
pp. 215-236
Author(s):  
Nadine Saul ◽  
Steffen Möller ◽  
Francesca Cirulli ◽  
Alessandra Berry ◽  
Walter Luyten ◽  
...  

AbstractSeveral biogerontology databases exist that focus on genetic or gene expression data linked to health as well as survival, subsequent to compound treatments or genetic manipulations in animal models. However, none of these has yet collected experimental results of compound-related health changes. Since quality of life is often regarded as more valuable than length of life, we aim to fill this gap with the “Healthy Worm Database” (http://healthy-worm-database.eu). Literature describing health-related compound studies in the aging model Caenorhabditis elegans was screened, and data for 440 compounds collected. The database considers 189 publications describing 89 different phenotypes measured in 2995 different conditions. Besides enabling a targeted search for promising compounds for further investigations, this database also offers insights into the research field of studies on healthy aging based on a frequently used model organism. Some weaknesses of C. elegans-based aging studies, like underrepresented phenotypes, especially concerning cognitive functions, as well as the convenience-based use of young worms as the starting point for compound treatment or phenotype measurement are discussed. In conclusion, the database provides an anchor for the search for compounds affecting health, with a link to public databases, and it further highlights some potential shortcomings in current aging research.


2021 ◽  
pp. 1-17
Author(s):  
Mani Iyer Prasanth ◽  
James Michael Brimson ◽  
Dicson Sheeja Malar ◽  
Anchalee Prasansuklab ◽  
Tewin Tencomnao

BACKGROUND: Streblus asper Lour., has been reported to have anti-aging and neuroprotective efficacies in vitro. OBJECTIVE: To analyze the anti-aging, anti-photoaging and neuroprotective efficacies of S. asper in Caenorhabditis elegans. METHODS: C. elegans (wild type and gene specific mutants) were treated with S. asper extract and analyzed for lifespan and other health benefits through physiological assays, fluorescence microscopy, qPCR and Western blot. RESULTS: The plant extract was found to increase the lifespan, reduce the accumulation of lipofuscin and modulate the expression of candidate genes. It could extend the lifespan of both daf-16 and daf-2 mutants whereas the pmk-1 mutant showed no effect. The activation of skn-1 was observed in skn-1::GFP transgenic strain and in qPCR expression. Further, the extract can extend the lifespan of UV-A exposed nematodes along with reducing ROS levels. Additionally, the extract also extends lifespan and reduces paralysis in Aβ transgenic strain, apart from reducing Aβ expression. CONCLUSIONS: S. asper was able to extend the lifespan and healthspan of C. elegans which was independent of DAF-16 pathway but dependent on SKN-1 and MAPK which could play a vital role in eliciting the anti-aging, anti-photoaging and neuroprotective effects, as the extract could impart oxidative stress resistance and neuroprotection.


2005 ◽  
Vol 288 (2) ◽  
pp. C467-C474 ◽  
Author(s):  
S. Todd Lamitina ◽  
Kevin Strange

All cells adapt to hypertonic stress by regulating their volume after shrinkage, by accumulating organic osmolytes, and by activating mechanisms that protect against and repair hypertonicity-induced damage. In mammals and nematodes, inhibition of signaling from the DAF-2/IGF-1 insulin receptor activates the DAF-16/FOXO transcription factor, resulting in increased life span and resistance to some types of stress. We tested the hypothesis that inhibition of insulin signaling in Caenorhabditis elegans also increases hypertonic stress resistance. Genetic inhibition of DAF-2 or its downstream target, the AGE-1 phosphatidylinositol 3-kinase, confers striking resistance to a normally lethal hypertonic shock in a DAF-16-dependent manner. However, insulin signaling is not inhibited by or required for adaptation to hypertonic conditions. Microarray studies have identified 263 genes that are transcriptionally upregulated by DAF-16 activation. We identified 14 DAF-16-upregulated genes by RNA interference screening that are required for age- 1 hypertonic stress resistance. These genes encode heat shock proteins, proteins of unknown function, and trehalose synthesis enzymes. Trehalose levels were elevated approximately twofold in age- 1 mutants, but this increase was insufficient to prevent rapid hypertonic shrinkage. However, age- 1 animals unable to synthesize trehalose survive poorly under hypertonic conditions. We conclude that increased expression of proteins that protect eukaryotic cells against environmental stress and/or repair stress-induced molecular damage confers hypertonic stress resistance in C. elegans daf- 2/ age- 1 mutants. Elevated levels of solutes such as trehalose may also function in a cytoprotective manner. Our studies provide novel insights into stress resistance in animal cells and a foundation for new studies aimed at defining molecular mechanisms underlying these essential processes.


Author(s):  
L. A. Nikanova ◽  

The influence of feed additive "Ormik" consisting of organic acids (formic acid, lactic acid, orthophosphoric acid, acetic acid), as a filler of diatomite crumb, introduced into concentrated fodders, on the biochemical parameters of blood serum of pigs and on productivity is considered in the article. The studied feed additive was added to the feed daily in the form of loose powder, mixing thoroughly. The initial dosage of the feed additive in the 1st week was 1 g/kg of feed, 2nd week and the following – 3 g/kg of feed. The average daily gain in the live weight of pigs in the experimental group was 11,0% higher than the control group. The animals of the experimental group were less sick and the safety of this group was 100%, in the control group it was 90%.


2018 ◽  
Vol 56 ◽  
pp. 136-140
Author(s):  
G. S. Sharapa ◽  
O. V. Boyko

Scientific and production experiments were carried out on cows of Ukrainian Black-and-White diary breed (UBAWD) of SE "Seagull" (branch "Lisne") during 2016–2017. The anatomical and physiological state of genital organs of cows was determined by the method of clinical and gynecological research of animals before conducting experiments and during them every 40–50 days. In total 486 cows were kept under control, which were kept in typical rooms with running platforms, but the active motivation of animals was clearly insufficient. Feeding cows according to the recommended standards. The milk yield from the cow in 2017 was 7813 kg. The experiments studied the effect of feeding cows during the dry period, the conditions of retention and some other paratypic factors on the course of the genera and the postnatal period, the duration of the service period and the fertility of cows. In analyzing the research results, personal records and veterinary documentation were used. In conducting experiments, it was found that normal calving was in 89% of cows, and severe and pathological – in 11% of cases. The main diseases in the afterlife period were: detention of the litter – 9.5%, postpartum endometritis – 21.5%, hypofunction of the ovaries – 23.0%, persistent yellow ovarian bodies – 25.6% and others. Normal intervals between stages of excitation of the sexual cycle (18–22 days) were in 63% of cows, and violations of the rhythm of sexual cycle, associated with dysfunction of the ovaries and missed the hunt – 30% of animals. Subunvulation of the genital organs of the cows contributed to the diseases of the limbs, udder and digestive disorders. During 2016–2017, the effect of feeding cows (173 g.) in the dry period on the course of calving, post-autumn period and their fertility was studied. Under the same conditions, keeping cows in the maternity ward and feeding rough and succulent feeds concentrated feeds fed cows of the control group (98 heads) to 1–2 kg daily during the dry period, and experimental (75 heads) – for 18–20 days to calving 2–3 kg. A positive difference was found in favor of cows of the experimental group: there was a more active stage of expulsion of the fetus, less delay of the placenta (by 7%) and endometritis (by 12%), the duration of the postoperative regenerative period decreased by 8.6 days; service period – 19.0 days; fertility from two inseminans was higher by 5.7%. Study of the reproductive capacity of cows at the hotel in different seasons showed that the recovery period was shorter by 11 days in the presence of cows in 3–4 quarters, and the service period – by 23.2 days at hotel in I and IV sq. The average duration of the recovery period was 79.5 days, while the service period was 123.4 days. The longest duration of the joint ventricle was in cows at hotel during the hot summer days of the year. The average fertilization rate from the first insemination of 339 cows was 44.9% with fluctuations in individual groups of milkers from 26.8% to 55.0%. The following indicators were in the number 1 and number 3 (respectively 26.8% and 31.4%). The best fertility rates were in groups № 4, 6 and 7, where there were more cows of the second and third lactation, and the responsible attitude of the milkers to work in organizing cows walks and sexually explicit hunting. When comparing the assessment of the duration of the IP and SP in full-age cows and cows, the first-born, taking into account 225 goals. It was found that these indicators were longer in primitive cows – 20.7 days respectively and 21.9 days in relation to full-age cows. The obtained results are completely consistent with the results obtained by a number of both domestic and foreign researchers. More attention is needed to grow heifers and to prepare newborns for delivery. In 15–17% of cows at and after the hotel, gynecological diseases that occurred in the presence of gastrointestinal diseases, lameness, mastitis and others were diagnosed. This has negatively affected the overall performance of the herd. In studying the duration of HP and SP in healthy and sick cows after calving, it was found that if the SP in healthy cows lasts 73.4 days, then in patients – 116.3 days (the difference was 42.9 days), and the difference in the duration of the joint venture reached 98.7 days. On the basis of the results of the research, it can be concluded that the reproductive function of the cows is influenced by such paratypic factors as feeding animals in the dry period, the temperature factor (time of year), the human factor (the effect of milking on a group of cows), age and physiological state of cows in the hotel and in the afterlife period. When feeding 2–3 kg of bovine feed for cows, together with rough and juicy fodder for 18–20 days prior to calving, the recovery period decreased by 8.6 days and service period – by 19 days, and fertility from two inseminals increased by 5.7%, reaching 85.3%. The smallest duration of the service-period was in the presence of cows in the I and IV quarters year (107.7–117.1 days). In primary cows, the recovery period was longer than that of full-age cows by 20.7 days, and the service period was 21.9 days. In the physiologically healthy cows after calving, the recovery period lasted an average of 73.4 days and service period – 98.7 days, and in patients, after the prescribed treatment – respectively 116.3 days and 197.4 days.


Author(s):  
Maryam Mousavi ◽  
Farshad Ghazalian

Introduction: Improving balance in the daily lives of the elderly plays an important role, especially in reducing their risk of falling. Therefore, the aim of the present study was to investigate the effect of eight weeks water resistance training with dark chocolate supplementation on the balance of the elderly.Methods: In this study, 38 elderly people with an age range of 73-60 years were randomly divided into four groups. Participants in groups, included water resistance training and water resistance training + dark chocolate, performed water resistance training for eight weeks. In this period, groups of dark chocolate and water resistance training + dark chocolate, consumed 6 pieces of dark chocolate 83%, 5 gr per day, and the control group did not have any training or supplementation. The stork test (flamingo) was used to assess balance. The results of covariance analysis (ANOVA) showed that there was a statistically significant difference between the four groups. Data were evaluated using Excel and SPSS-25 (p≤ 0.05). Results: The adjusted means after eliminating the effect of pre-test scores showed that the water resistance-training group had a higher mean than the other three groups and the weakest scores belonged to the control group. The results of other groups were as followed: experimental group 18.77, chocolate group 16.88, combined group (water resistance training + dark chocolate) 17.24 and control group 9.77. The results of Benferoni test showed that there was a significant difference between the water resistance training group and the control group (p = 0.006). Conclusion: According to the results of this study, it seems that eight weeks of water resistance training and dark chocolate supplementation can improve the balance and quality of life of the elderly.


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