scholarly journals Identification of GA-Binding Protein Transcription Factor Alpha Subunit (GABPA) as a Novel Bookmarking Factor

2019 ◽  
Vol 20 (5) ◽  
pp. 1093 ◽  
Author(s):  
Shunya Goto ◽  
Masashi Takahashi ◽  
Narumi Yasutsune ◽  
Sumiki Inayama ◽  
Dai Kato ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Histone Acetylation ◽  
Binding Protein ◽  
Alpha Subunit ◽  
Upstream Region ◽  
G1 Phase ◽  
Sp1 Transcription Factor ◽  
Factor Alpha ◽  
Ga Binding Protein ◽  
Protein Transcription

Mitotic bookmarking constitutes a mechanism for transmitting transcriptional patterns through cell division. Bookmarking factors, comprising a subset of transcription factors (TFs), and multiple histone modifications retained in mitotic chromatin facilitate reactivation of transcription in the early G1 phase. However, the specific TFs that act as bookmarking factors remain largely unknown. Previously, we identified the “early G1 genes” and screened TFs that were predicted to bind to the upstream region of these genes, then identified GA-binding protein transcription factor alpha subunit (GABPA) and Sp1 transcription factor (SP1) as candidate bookmarking factors. Here we show that GABPA and multiple histone acetylation marks such as H3K9/14AC, H3K27AC, and H4K5AC are maintained at specific genomic sites in mitosis. During the M/G1 transition, the levels of these histone acetylations at the upstream regions of genes bound by GABPA in mitosis are decreased. Upon depletion of GABPA, levels of histone acetylation, especially H4K5AC, at several gene regions are increased, along with transcriptional induction at 1 h after release. Therefore, we proposed that GABPA cooperates with the states of histone acetylation to act as a novel bookmarking factor which, may negatively regulate transcription during the early G1 phase.

scholarly journals Human Lineage-Specific Transcriptional Regulation through GA-Binding Protein Transcription Factor Alpha (GABPa)

2016 ◽  
Vol 33 (5) ◽  
pp. 1231-1244 ◽  
Author(s):  
Alvaro Perdomo-Sabogal ◽  
Katja Nowick ◽  
Ilaria Piccini ◽  
Ralf Sudbrak ◽  
Hans Lehrach ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcriptional Regulation ◽  
Binding Protein ◽  
Human Lineage ◽  
Factor Alpha ◽  
Ga Binding Protein ◽  
Protein Transcription

scholarly journals Function of a Transcription Factor CDX2 Beyond Its Trophectoderm Lineage Specification

Endocrinology ◽  
2010 ◽  
Vol 151 (12) ◽  
pp. 5873-5881 ◽  
Author(s):  
Toshihiro Sakurai ◽  
Hanako Bai ◽  
Toshihiro Konno ◽  
Atsushi Ideta ◽  
Yoshito Aoyagi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Protein Binding ◽  
Histone Acetylation ◽  
Chromatin Immunoprecipitation ◽  
Binding Protein ◽  
Camp Response Element Binding ◽  
Upstream Region ◽  
Camp Response Element ◽  
Response Element ◽  
Element Binding Protein

The transcription factor caudal-related homeobox 2 (CDX2) regulates trophectoderm differentiation, but its function beyond trophectoderm differentiation is not well characterized. CDX2 was shown to regulate a trophoblast-specific gene, interferon τ (IFNT), in the ruminants. However, its regulatory mechanism has not been determined. Here, we report a new role of CDX2 in histone modifications of the IFNT gene. Chromatin immunoprecipitation assays using ovine conceptuses obtained from d 14, 16, 16.5, or 20 of pregnancy (d 0, day of mating) revealed that H3K18 acetylation was highly detectable at the upstream and open reading frame regions of the IFNT gene on d 14 and 16, when CDX2 reached its peak expression. From d 16.5, when the conceptus initiates attachment to uterine epithelial cells, histone acetylation along with CDX2 expression declines. Two candidate CDX2 binding sites (−300 to −294 bp and −293 to −287 bp) of the bovine IFNT gene promoter region were detected from chromatin immunoprecipitation and luciferase assay. When Cdx2 constructs were transfected into bovine ear-derived fibroblast cells, histone acetylation was increased, concurrent with the recruitment of cAMP response element binding protein-binding protein, which has histone acetyltransferase activity. H3K18 acetylation was seen in the proximity of the CDX2 binding region located at the IFNT gene’s upstream region in CT-1 cells, but when these cells were treated with specific CDX2 small interfering RNA, H3K18 acetylation was decreased. These findings suggest that CDX2 regulates its targeted gene through cAMP response element binding protein-binding protein recruitment, which correlates with greater histone acetylation.

scholarly journals Functional Interactions of Transcription Factor Human GA-binding Protein Subunits

1998 ◽  
Vol 273 (45) ◽  
pp. 29302-29308 ◽  
Author(s):  
Fumihiko Suzuki ◽  
Masahide Goto ◽  
Chika Sawa ◽  
Seiichiro Ito ◽  
Hajime Watanabe ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Binding Protein ◽  
Protein Subunits ◽  
Functional Interactions ◽  
Ga Binding Protein
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