scholarly journals Mechanisms of Toxicity of Industrially Relevant Silicomanganese Dust on Human 1321N1 Astrocytoma Cells: An In Vitro Study

2019 ◽  
Vol 20 (3) ◽  
pp. 740
Author(s):  
Yke Arnoldussen ◽  
Torunn Kringlen Ervik ◽  
Johanna Samulin Erdem ◽  
Ida Kero ◽  
Mina Baarnes Eriksen ◽  
...  
Keyword(s):  
Intercellular Communication ◽  
Biological Effects ◽  
In Vitro Study ◽  
Dust Particles ◽  
Gap Junctional Intercellular Communication ◽  
Cellular Mechanisms ◽  
Mechanisms Of Toxicity ◽  
Time Dependent Effect ◽  
Gap Junctional

Tremendous efforts are applied in the ferroalloy industry to control and reduce exposure to dust generated during the production process, as inhalable Mn-containing particulate matter has been linked to neurodegenerative diseases. This study aimed to investigate the toxicity and biological effects of dust particles from laboratory-scale processes where molten silicomanganese (SiMn) was exposed to air, using a human astrocytoma cell line, 1321N1, as model system. Characterization of the dust indicated presence of both nano-sized and larger particles averaging between 100 and 300 nm. The dust consisted mainly of Si, Mn and O. Investigation of cellular mechanisms showed a dose- and time-dependent effect on cell viability, with only minor changes in the expression of proteins involved in apoptosis. Moreover, gene expression of the neurotoxic biomarker amyloid precursor protein (APP) increased, whereas APP protein expression decreased. Finally, induction of gap junctional intercellular communication (GJIC) increased with higher doses and correlated with the other endpoints. Thus, the effects of SiMn dust on 1321N1 cells are highly dependent on the dose of exposure and involves changes in APP, apoptosis-related proteins and intercellular communication.

Mutagenic and epigenetic mechanisms of zearalenone: prevention by Vitamin E

2008 ◽  
Vol 1 (3) ◽  
pp. 369-374 ◽  
Author(s):  
Z. Ouanes-Ben Othmen ◽  
S. Essefi ◽  
H. Bacha
Keyword(s):  
Vitamin E ◽  
Intercellular Communication ◽  
Chromosome Aberrations ◽  
Vero Cells ◽  
Antioxidant Vitamin ◽  
Epigenetic Mechanisms ◽  
Gap Junctional Intercellular Communication ◽  
Epigenetic Effects ◽  
Gap Junctional

It has been suggested that zearalenone, a non-steroidal estrogenic mycotoxin produced by Fusarium graminearium, causes DNA damage. However, the mutagenic properties of this toxin are controversial. The purpose of this study was to investigate both genotoxic and epigenetic effects of zearalenone in vitro. The effects of zearalenone on unscheduled DNA synthesis (UDS), induction of chromosome aberrations and inhibition of gap junctional intercellular communication were determined using Vero cells. The results show that in Vero cells, zearalenone treatment caused a concentration-dependent increase in UDS, induced chromosome aberrations and inhibited gap junctional intercellular communication. All of these effects were either prevented or reduced by co-treatment with the antioxidant vitamin E. The results support the hypothesis that in Vero cells zearalenone-induced oxidative stress is involved in and precedes all of the studied effects.

scholarly journals Controlling cell–cell interactions using surface acoustic waves

2014 ◽  
Vol 112 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Feng Guo ◽  
Peng Li ◽  
Jarrod B. French ◽  
Zhangming Mao ◽  
Hong Zhao ◽  
...  
Keyword(s):  
Intercellular Communication ◽  
Acoustic Waves ◽  
Cancer Metastasis ◽  
Surface Acoustic Waves ◽  
Spatial Arrangement ◽  
Gap Junctional Intercellular Communication ◽  
Wave Technique ◽  
Immunological Reactions ◽  
Gap Junctional

The interactions between pairs of cells and within multicellular assemblies are critical to many biological processes such as intercellular communication, tissue and organ formation, immunological reactions, and cancer metastasis. The ability to precisely control the position of cells relative to one another and within larger cellular assemblies will enable the investigation and characterization of phenomena not currently accessible by conventional in vitro methods. We present a versatile surface acoustic wave technique that is capable of controlling the intercellular distance and spatial arrangement of cells with micrometer level resolution. This technique is, to our knowledge, among the first of its kind to marry high precision and high throughput into a single extremely versatile and wholly biocompatible technology. We demonstrated the capabilities of the system to precisely control intercellular distance, assemble cells with defined geometries, maintain cellular assemblies in suspension, and translate these suspended assemblies to adherent states, all in a contactless, biocompatible manner. As an example of the power of this system, this technology was used to quantitatively investigate the gap junctional intercellular communication in several homotypic and heterotypic populations by visualizing the transfer of fluorescent dye between cells.

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