Lactic acid inhibition of gap junctional intercellular communication in in vitro astrocytes as measured by fluorescence recovery after laser photobleaching

Glia ◽  
1988 ◽  
Vol 1 (6) ◽  
pp. 371-379 ◽  
Author(s):  
Juanita J. Anders
Keyword(s):  
Lactic Acid ◽  
Intercellular Communication ◽  
Acid Inhibition ◽  
Fluorescence Recovery ◽  
Gap Junctional Intercellular Communication ◽  
Gap Junctional

Mutagenic and epigenetic mechanisms of zearalenone: prevention by Vitamin E

2008 ◽  
Vol 1 (3) ◽  
pp. 369-374 ◽  
Author(s):  
Z. Ouanes-Ben Othmen ◽  
S. Essefi ◽  
H. Bacha
Keyword(s):  
Vitamin E ◽  
Intercellular Communication ◽  
Chromosome Aberrations ◽  
Vero Cells ◽  
Antioxidant Vitamin ◽  
Epigenetic Mechanisms ◽  
Gap Junctional Intercellular Communication ◽  
Epigenetic Effects ◽  
Gap Junctional

It has been suggested that zearalenone, a non-steroidal estrogenic mycotoxin produced by Fusarium graminearium, causes DNA damage. However, the mutagenic properties of this toxin are controversial. The purpose of this study was to investigate both genotoxic and epigenetic effects of zearalenone in vitro. The effects of zearalenone on unscheduled DNA synthesis (UDS), induction of chromosome aberrations and inhibition of gap junctional intercellular communication were determined using Vero cells. The results show that in Vero cells, zearalenone treatment caused a concentration-dependent increase in UDS, induced chromosome aberrations and inhibited gap junctional intercellular communication. All of these effects were either prevented or reduced by co-treatment with the antioxidant vitamin E. The results support the hypothesis that in Vero cells zearalenone-induced oxidative stress is involved in and precedes all of the studied effects.

scholarly journals Controlling cell–cell interactions using surface acoustic waves

2014 ◽  
Vol 112 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Feng Guo ◽  
Peng Li ◽  
Jarrod B. French ◽  
Zhangming Mao ◽  
Hong Zhao ◽  
...  
Keyword(s):  
Intercellular Communication ◽  
Acoustic Waves ◽  
Cancer Metastasis ◽  
Surface Acoustic Waves ◽  
Spatial Arrangement ◽  
Gap Junctional Intercellular Communication ◽  
Wave Technique ◽  
Immunological Reactions ◽  
Gap Junctional

The interactions between pairs of cells and within multicellular assemblies are critical to many biological processes such as intercellular communication, tissue and organ formation, immunological reactions, and cancer metastasis. The ability to precisely control the position of cells relative to one another and within larger cellular assemblies will enable the investigation and characterization of phenomena not currently accessible by conventional in vitro methods. We present a versatile surface acoustic wave technique that is capable of controlling the intercellular distance and spatial arrangement of cells with micrometer level resolution. This technique is, to our knowledge, among the first of its kind to marry high precision and high throughput into a single extremely versatile and wholly biocompatible technology. We demonstrated the capabilities of the system to precisely control intercellular distance, assemble cells with defined geometries, maintain cellular assemblies in suspension, and translate these suspended assemblies to adherent states, all in a contactless, biocompatible manner. As an example of the power of this system, this technology was used to quantitatively investigate the gap junctional intercellular communication in several homotypic and heterotypic populations by visualizing the transfer of fluorescent dye between cells.

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