scholarly journals WRKYs, the Jack-of-various-Trades, Modulate Dehydration Stress in Populus davidiana—A Transcriptomic Approach

2019 ◽  
Vol 20 (2) ◽  
pp. 414 ◽  
Author(s):  
Qari Imran ◽  
Sang-Uk Lee ◽  
Bong-Gyu Mun ◽  
Adil Hussain ◽  
Sajjad Asaf ◽  
...  
Keyword(s):  
Forest Cover ◽  
Dehydration Stress ◽  
Rna Seq ◽  
Populus Davidiana ◽  
Central Asian ◽  
Knockout Mutants ◽  
In Vivo Analysis ◽  
Central Asian Countries

Populus davidiana, native to Korea and central Asian countries, is a major contributor to the Korean forest cover. In the current study, using high-throughput RNA-seq mediated transcriptome analysis, we identified about 87 P. davidiana WRKY transcription factors (PopdaWRKY TFs) that showed differential expression to dehydration stress in both sensitive and tolerant cultivars. Our results suggested that, on average, most of the WRKY genes were upregulated in tolerant cultivars but downregulated in sensitive cultivars. Based on protein sequence alignment, P. davidiana WRKYs were classified into three major groups, I, II, III, and further subgroups. Phylogenetic analysis showed that WRKY TFs and their orthologs in Arabidopsis and rice were clustered together in the same subgroups, suggesting similar functions across species. Significant correlation was found among qRT-PCR and RNA-seq analysis. In vivo analysis using model plant Arabidopsis showed that atwrky62 (orthologous to Potri.016G137900) knockout mutants were significantly sensitive to dehydration possibly due to an inability to close their stomata under dehydration conditions. In addition, a concomitant decrease in expression of ABA biosynthetic genes was observed. The AtHK1 that regulates stomatal movement was also downregulated in atwrky62 compared to the wild type. Taken together, our findings suggest a regulatory role of PopdaWRKYs under dehydration stress.

Meiotic CENP-C is a shepherd: bridging the space between the centromere and the kinetochore in time and space

2020 ◽  
Vol 64 (2) ◽  
pp. 251-261
Author(s):  
Jessica E. Fellmeth ◽  
Kim S. McKim
Keyword(s):  
Chromosome Segregation ◽  
New Technologies ◽  
Early Prophase ◽  
Unique Role ◽  
Kinetochore Assembly ◽  
M Phase ◽  
In Vivo Analysis ◽  
Meiosis I

Abstract While many of the proteins involved in the mitotic centromere and kinetochore are conserved in meiosis, they often gain a novel function due to the unique needs of homolog segregation during meiosis I (MI). CENP-C is a critical component of the centromere for kinetochore assembly in mitosis. Recent work, however, has highlighted the unique features of meiotic CENP-C. Centromere establishment and stability require CENP-C loading at the centromere for CENP-A function. Pre-meiotic loading of proteins necessary for homolog recombination as well as cohesion also rely on CENP-C, as do the main scaffolding components of the kinetochore. Much of this work relies on new technologies that enable in vivo analysis of meiosis like never before. Here, we strive to highlight the unique role of this highly conserved centromere protein that loads on to centromeres prior to M-phase onset, but continues to perform critical functions through chromosome segregation. CENP-C is not merely a structural link between the centromere and the kinetochore, but also a functional one joining the processes of early prophase homolog synapsis to late metaphase kinetochore assembly and signaling.

RUSSIA AND CENTRAL ASIAN COUNTRIES IN YOUTH EXCHANGES WITHIN THE SCO

2021 ◽  
Vol 66 (1) ◽  
pp. 115-121
Author(s):  
KSENIA G. MURATSHINA ◽  
◽  
EVGENY L. BAKHTIN ◽  
Keyword(s):  
International Relations ◽  
Online News ◽  
Asian Countries ◽  
Multilateral Cooperation ◽  
Central Asian ◽  
Cooperation Mechanism ◽  
Potential Benefits ◽  
Central Asian Countries ◽  
Shanghai Cooperation Organisation

Youth exchanges have become an essential component of people-to-people exchanges in international relations, both in bilateral and multilateral formats. This paper analyses the participation of Russia and its Central Asian partners (Kazakhstan, Kyrgyzstan, Tajikistan and Uzbekistan) in youth exchanges in the Shanghai Cooperation Organisation. The authors consequently discuss the institutional structure and formats of the multilateral cooperation, its development within the last decade, and the representation and role of the member states. The research is based on the study of the SCO multilateral documents, SCO institutions’ documentation, official addresses and interviews of the SCO politicians and NGO leaders, online news archives of NGOs and media. The authors conclude that the SCO has formed a multilateral cooperation mechanism for youth exchanges, in which Russia and Central Asia are fully represented, with minor exclusions. Meanwhile, the cooperation demonstrates the evident rivalry between Russia and China in this cooperation. The Central Asian countries have become subject to this rivalry, however, at the same time they have already started to put forward their own initiatives, too, which can be significant for the development of multilateral dialogue. Finally, the paper discusses the potential benefits of cooperation for its participants.

scholarly journals Requirement for PKC Epsilon in Kras-Driven Lung Tumorigenesis

2020 ◽  
Author(s):  
Rachana Garg ◽  
Mariana Cooke ◽  
Shaofei Wang ◽  
Fernando Benavides ◽  
Martin C. Abba ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lung Adenocarcinoma ◽  
Cancer Therapeutics ◽  
Rna Seq ◽  
Lung Tumorigenesis ◽  
Mechanistic Analysis ◽  
Oncogenic Driver ◽  
Histological Form

ABSTRACTNon-small cell lung cancer (NSCLC), the most frequent subtype of lung cancer, remains a highly lethal malignancy and one of the leading causes of cancer deaths worldwide. Mutant KRAS is the prevailing oncogenic driver of lung adenocarcinoma, the most common histological form of NSCLC. In this study, we examined the role of PKCε, an oncogenic kinase highly expressed in NSCLC and other cancers, in KRAS-driven tumorigenesis. Notably, database analysis revealed an association between PKCε expression and poor outcome in lung adenocarcinoma patients specifically having KRAS mutation. By generating a PKCε-deficient, conditionally activatable allele of oncogenic Kras (LSL-KrasG12D;PKCε−/− mice) we were able to demonstrate the requirement of PKCε for Kras-driven lung tumorigenesis in vivo, which is consistent with the impaired transformed growth observed in PKCε-deficient KRAS-dependent NSCLC cells. Moreover, PKCε-knockout mice were found to be less susceptible to lung tumorigenesis induced by benzo[a]pyrene, a carcinogen that induces mutations in Kras. Mechanistic analysis using RNA-Seq revealed little overlapping for PKCε and KRAS in the control of genes/biological pathways relevant in NSCLC, suggesting that a permissive role of PKCε in KRAS-driven lung tumorigenesis may involve non-redundant mechanisms. Our results thus highlight the relevance and potential of targeting PKCε for lung cancer therapeutics.

scholarly journals The role of the NMD factor UPF3B in olfactory sensory neurons

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Kun Tan ◽  
Samantha H Jones ◽  
Blue B Lake ◽  
Jennifer N Dumdie ◽  
Eleen Y Shum ◽  
...  
Keyword(s):  
Human Cognition ◽  
Cell Populations ◽  
Rna Seq ◽  
Wild Type ◽  
Single Cell Rna Sequencing ◽  
Nonsense Mediated Rna Decay ◽  
Different Levels ◽  
Selection Of

The UPF3B-dependent branch of the nonsense-mediated RNA decay (NMD) pathway is critical for human cognition. Here, we examined the role of UPF3B in the olfactory system. Single-cell RNA-sequencing (scRNA-seq) analysis demonstrated considerable heterogeneity of olfactory sensory neuron (OSN) cell populations in wild-type (WT) mice, and revealed that UPF3B loss influences specific subsets of these cell populations. UPF3B also regulates the expression of a large cadre of antimicrobial genes in OSNs, and promotes the selection of specific olfactory receptor (Olfr) genes for expression in mature OSNs (mOSNs). RNA-seq and Ribotag analyses identified classes of mRNAs expressed and translated at different levels in WT and Upf3b-null mOSNs. Integrating multiple computational approaches, UPF3B-dependent NMD target transcripts that are candidates to mediate the functions of NMD in mOSNs were identified in vivo. Together, our data provides a valuable resource for the olfactory field and insights into the roles of NMD in vivo.

Central Asia After Three Decades of Independence

2021 ◽  
Keyword(s):  
Social Capital ◽  
Civil Society ◽  
Central Asia ◽  
Gender Equality ◽  
International Security ◽  
People With Disabilities ◽  
Asian Countries ◽  
Central Asian ◽  
Central Asian Countries

On the 30th anniversary of the dissolution of the USSR, this book collects selected contributions which analyse patterns of stability and transformation that characterise the politics and societies of three Central Asian countries—Kazakhstan, Kyrgyzstan and Uzbekistan—along with those of Central Asia as a region. In particular, this edited volume investigates gender equality discourses in Uzbekistan, the electoral rights of people with disabilities in Kyrgyzstan, neo-realism in the regional context of Central Asia, the role of Islam in Kyrgyzstan and Uzbekistan, Kazakhstan as a provider of international security as well as the EU’s support of civil society and social capital in Kazakhstan. With contributions by Nora Becker, Dr. Shalva Dzebisashvili, Aziz Elmuradov, Prof. Dr. Matthias Kortmann, Dr. Aliia Maralbaeva, Laura Karoline Nette, Dr. Chiara Pierobon and Dr. Steve Schlegel.

scholarly journals Tajikistan’s Perspective on Eurasia

2019 ◽  
Vol 75 (1) ◽  
pp. 100-107
Author(s):  
Jovid Ikromov
Keyword(s):  
South Asia ◽  
Central Asia ◽  
World Economy ◽  
Asian Countries ◽  
The West ◽  
Eurasian Continent ◽  
Central Asian ◽  
The World ◽  
Central Asian Countries

In this article, the place of Central Asia, particularly of Tajikistan, in the Eurasian continent has been examined. The slow and confident transfer of engine of the world economy from the West to the East and South increasing the role of the countries located between them. Located between Europe, Russia and South Asia, five Central Asian countries are interested in the development and participation in broader transcontinental trade and transit corridors connecting in all directions. Tajikistan has a unique opportunity to become a hub of trade and transit as it is located at the crossroads of growing ties between South and Central Asia.

scholarly journals MIR2111-5 locus and shoot-accumulated mature miR2111 systemically enhance nodulation depending on HAR1 in Lotus japonicus

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Nao Okuma ◽  
Takashi Soyano ◽  
Takuya Suzaki ◽  
Masayoshi Kawaguchi
Keyword(s):  
Root Nodules ◽  
Lotus Japonicus ◽  
Dependent Manner ◽  
Rna Seq ◽  
Signaling System ◽  
Nodule Number ◽  
Knockout Mutants ◽  
Mutualistic Relationship ◽  
Dose Dependent

Abstract Legumes utilize a shoot-mediated signaling system to maintain a mutualistic relationship with nitrogen-fixing bacteria in root nodules. In Lotus japonicus, shoot-to-root transfer of microRNA miR2111 that targets TOO MUCH LOVE, a nodulation suppressor in roots, has been proposed to explain the mechanism underlying nodulation control from shoots. However, the role of shoot-accumulating miR2111s for the systemic regulation of nodulation was not clearly shown. Here, we find L. japonicus has seven miR2111 loci, including those mapped through RNA-seq. MIR2111-5 expression in leaves is the highest among miR2111 loci and repressed after rhizobial infection depending on a shoot-acting HYPERNODULATION ABERRANT ROOT FORMATION1 (HAR1) receptor. MIR2111-5 knockout mutants show significantly decreased nodule numbers and miR2111 levels. Furthermore, grafting experiments using transformants demonstrate scions with altered miR2111 levels influence nodule numbers in rootstocks in a dose-dependent manner. Therefore, miR2111 accumulation in leaves through MIR2111-5 expression is required for HAR1-dependent systemic optimization of nodule number.

scholarly journals RIVET—A Tool for In Vivo Analysis of Symbiotically Relevant Gene Expression in Sinorhizobium meliloti

2008 ◽  
Vol 21 (2) ◽  
pp. 162-170 ◽  
Author(s):  
Mengsheng Gao ◽  
Max Teplitski
Keyword(s):  
Gene Regulation ◽  
Sinorhizobium Meliloti ◽  
Homoserine Lactone ◽  
Symbiotic Gene ◽  
Gus Reporter ◽  
In Vivo Analysis ◽  
Plant Signals ◽  
Relevant Gene

Despite significant advances in the development of sensitive tools for studying genetics and signal exchange in legume–rhizobium symbioses, many uncertainties remain about the in vivo role of bacterial and plant signals in symbiotic gene regulation. In this study, we adapted TnpR recombinase-based in vivo expression technology (RIVET) to document gene regulation in Sinorhizobium meliloti. The substrate for TnpR, the res1-tet-res1 cassette, is stably inherited when cloned into a neutral site of the S. meliloti genome. Bicistronic promoterless tnpR-β-glucuronidase (GUS) reporters were constructed to track expression (“resolution”) of symbiotically relevant S. meliloti genes during different stages of the interaction. In proof of principle experiments, the resolution of the nodC::tnpR reporter was detected within 4 h of exposure to micromolar levels of the nod operon inducer luteolin and after overnight incubation in the rhizosphere. RIVET demonstrated that cell division gene ftsZ2 was not strongly expressed in the rhizosphere but was activated inside the nodules and on agar surfaces. Rhizosphere expression of the N-acyl homoserine lactone (AHL) synthase sinI::tnpR-GUS reporter was modest in prequorate microcolonies, and then increased with time. AHL synthase sinI and an AHL-regulated gene, expG, were activated inside the nodules.

Apoptotic Role of Fas/Fas Ligand System in the Regulation of Erythropoiesis

Blood ◽  
1999 ◽  
Vol 93 (3) ◽  
pp. 796-803 ◽  
Author(s):  
R. De Maria ◽  
U. Testa ◽  
L. Luchetti ◽  
A. Zeuner ◽  
G. Stassi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Fas Ligand ◽  
Control Mechanism ◽  
Erythroid Differentiation ◽  
Dependent Manner ◽  
Normal Bone ◽  
In Vivo Analysis ◽  
Induced Apoptosis

The possible involvement of Fas and Fas ligand (FasL) in the regulation of erythropoiesis was evaluated. Immunohistochemistry of normal bone marrow specimens revealed that several immature erythroblasts undergo apoptosis in vivo. Analysis of bone marrow erythroblasts and purified progenitors undergoing unilineage erythroid differentiation showed that Fas is rapidly upregulated in early erythroblasts and expressed at high levels through terminal maturation. However, Fas crosslinking was effective only in less mature erythroblasts, particularly at basophilic level, where it induced apoptosis antagonized by high levels of erythropoietin (Epo). In contrast, FasL was selectively induced in late differentiating Fas-insensitive erythroblasts, mostly at the orthochromatic stage. FasL is functional in mature erythroblasts, as it was able to kill Fas-sensitive lymphoblast targets in a Fas-dependent manner. Importantly, FasL-bearing mature erythroblasts displayed a Fas-based cytotoxicity against immature erythroblasts, which was abrogated by high levels of Epo. These findings suggest the existence of a negative regulatory feedback between mature and immature erythroid cells, whereby the former cell population might exert a cytotoxic effect on the latter one in the erythroblastic island. Hypothetically, this negative feedback operates at low Epo levels to moderate the erythropoietic rate; however, it is gradually inhibited at increasing Epo concentrations coupled with enhanced erythrocyte production. Thus, the interaction of Fas and FasL may represent an apoptotic control mechanism for erythropoiesis, contributing to the regulation of red blood cell homeostasis.

Sign in / Sign up

Export Citation Format

Share Document