scholarly journals OsMGT1 Confers Resistance to Magnesium Deficiency By Enhancing the Import of Mg in Rice

2019 ◽  
Vol 20 (1) ◽  
pp. 207 ◽  
Author(s):  
Ludan Zhang ◽  
Yuyang Peng ◽  
Jian Li ◽  
Xinyue Tian ◽  
Zhichang Chen
Keyword(s):  
Plant Growth ◽  
Molecular Mechanisms ◽  
Magnesium Deficiency ◽  
Expression Patterns ◽  
Tissue Expression ◽  
Mesophyll Cells ◽  
Mg Deficiency ◽  
Biochemical Processes ◽  
Essential Nutrient ◽  
Mg Content

Magnesium (Mg) is an essential nutrient element for plant growth and plays an important role in numerous physiological and biochemical processes. Mg deficiency inhibits plant growth and has become a growing problem for crop productions in agriculture. However, the molecular mechanisms for the resistance to Mg deficiency in plants were not well understood. In this study, we identified a Mg transporter gene OsMGT1 that confers resistance to Mg deficiency in rice (Oryza sativa). The expression of OsMGT1 was highly induced by Mg deficiency in shoots. Investigation of tissue expression patterns revealed that OsMGT1 was mainly expressed in the phloem region; however, Mg deficiency remarkably enhanced its expression in xylem parenchyma and mesophyll cells in shoots. Knockout of OsMGT1 resulted in a significant reduction in Mg content and biomass when grown at Mg-limited conditions. Furthermore, the sensitivity to low-Mg in mutants was intensified by excessive calcium supply. In addition, overexpression of OsMGT1 increased Mg content and biomass under low-Mg supply. In conclusion, our results indicate that OsMGT1 plays an important role in rice Mg import and is required for the resistance to Mg deficiency, which can be utilized for molecular breeding of low-Mg tolerant plants.

Effects of magnesium deficiency on needle ultrastructure and growth of Scots pine seedlings

1995 ◽  
Vol 25 (11) ◽  
pp. 1806-1814 ◽  
Author(s):  
Virpi Palomäki
Keyword(s):  
Scots Pine ◽  
Magnesium Deficiency ◽  
Control Level ◽  
Growth Period ◽  
Mesophyll Cells ◽  
Mg Deficiency ◽  
Growing Period ◽  
Pine Seedlings ◽  
Second Growth ◽  
Mg Content

Effects of magnesium (Mg) deficiency on 3-year-old Scots pine (Pinussylvestris L.) seedlings were studied during a field experiment extending over two growth periods. Seedlings were grown in quartz sand in 7.5-L pots watered with nutrient solutions in which the Mg content was reduced to 30 and 0% of the control level (15 mg/L in the first and 3 mg/L in the second growth period). During the first growing period the Mg content of needles at the 0% Mg level was significantly lower than at the 30% level, and in the second growth period a decline was clear at both deficiency levels. Swelling of phloem cells was observed in samples taken after 4 weeks' exposure, and the frequency of swollen phloem cells increased towards autumn and through the second growth period. At the end of the first growing period a decrease in the number of thylakoids per granum and an increase in the number of plastoglobuli in chloroplasts of mesophyll cells were detected at both deficiency levels. During the second growing period the tips of needles from the previous year in the 0% Mg level group became brown, and these needles were shed in autumn from some of the seedlings. The chloroplasts in these needles were rounded and the thylakoids were abnormally organized. Structural symptoms caused by Mg deficiency were observable before visual changes and before a clear decrease in Mg content had occurred, thus showing the value of structural observations in early diagnosis of this stress.

Molecular and physiological mechanisms underlying magnesium-deficiency-induced enlargement, cracking and lignification of Citrus sinensis leaf veins

2020 ◽  
Vol 40 (9) ◽  
pp. 1277-1291 ◽  
Author(s):  
Xin Ye ◽  
Xu-Feng Chen ◽  
Li-Ya Cai ◽  
Ning-Wei Lai ◽  
Chong-Ling Deng ◽  
...  
Keyword(s):  
Cell Wall ◽  
Citrus Sinensis ◽  
Molecular Mechanisms ◽  
Rho Gtpase ◽  
Magnesium Deficiency ◽  
Lignin Biosynthesis ◽  
Starch Accumulation ◽  
Ascorbate Oxidase ◽  
Mg Deficiency ◽  
Leaf Veins

Abstract Little is known about the physiological and molecular mechanisms underlying magnesium (Mg)-deficiency-induced enlargement, cracking and lignification of midribs and main lateral veins of Citrus leaves. Citrus sinensis (L.) Osbeck seedlings were irrigated with nutrient solution at a concentration of 0 (Mg-deficiency) or 2 (Mg-sufficiency) mM Mg(NO3)2 for 16 weeks. Enlargement, cracking and lignification of veins occurred only in lower leaves, but not in upper leaves. Total soluble sugars (glucose + fructose + sucrose), starch and cellulose concentrations were less in Mg-deficiency veins of lower leaves (MDVLL) than those in Mg-sufficiency veins of lower leaves (MSVLL), but lignin concentration was higher in MDVLL than that in MSVLL. However, all four parameters were similar between Mg-deficiency veins of upper leaves (MDVUL) and Mg-sufficiency veins of upper leaves (MSVUL). Using label-free, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, we identified 1229 and 492 differentially abundant proteins (DAPs) in MDVLL vs MSVLL and MDVUL vs MSVUL, respectively. Magnesium-deficiency-induced alterations of Mg, nonstructural carbohydrates, cell wall components, and protein profiles were greater in veins of lower leaves than those in veins of upper leaves. The increased concentration of lignin in MDVLL vs MSVLL might be caused by the following factors: (i) repression of cellulose and starch accumulation promoted lignin biosynthesis; (ii) abundances of proteins involved in phenylpropanoid biosynthesis pathway, hormone biosynthesis and glutathione metabolism were increased; and (iii) the abundances of the other DAPs [viz., copper/zinc-superoxide dismutase, ascorbate oxidase (AO) and ABC transporters] involved in lignin biosynthesis were elevated. Also, the abundances of several proteins involved in cell wall metabolism (viz., expansins, Rho GTPase-activating protein gacA, AO, monocopper oxidase-like protein and xyloglucan endotransglucosylase/hydrolase) were increased in MDVLL vs MSVLL, which might be responsible for the enlargement and cracking of leaf veins.

Molecular mechanisms for magnesium-deficiency-induced leaf vein lignification, enlargement and cracking in Citrus sinensis revealed by RNA-Seq

2020 ◽  
Author(s):  
Xin Ye ◽  
Hui-Yu Huang ◽  
Feng-Lin Wu ◽  
Li-Ya Cai ◽  
Ning-Wei Lai ◽  
...  
Keyword(s):  
Cell Wall ◽  
Citrus Sinensis ◽  
Molecular Mechanisms ◽  
Magnesium Deficiency ◽  
Lignin Biosynthesis ◽  
Common Elements ◽  
Rna Seq ◽  
Transcript Levels ◽  
Mg Deficiency ◽  
Leaf Vein

Abstract Citrus sinensis (L.) Osbeck seedlings were fertigated with nutrient solution containing 2 [magnesium (Mg)-sufficiency] or 0 mM (Mg-deficiency) Mg(NO3)2 for 16 weeks. Thereafter, RNA-Seq was used to investigate Mg-deficiency-responsive genes in the veins of upper and lower leaves in order to understand the molecular mechanisms for Mg-deficiency-induced vein lignification, enlargement and cracking, which appeared only in the lower leaves. In this study, 3065 upregulated and 1220 downregulated, and 1390 upregulated and 375 downregulated genes were identified in Mg-deficiency veins of lower leaves (MDVLL) vs Mg-sufficiency veins of lower leaves (MSVLL) and Mg-deficiency veins of upper leaves (MDVUL) vs Mg-sufficiency veins of upper leaves (MSVUL), respectively. There were 1473 common differentially expressed genes (DEGs) between MDVLL vs MSVLL and MDVUL vs MSVUL, 1463 of which displayed the same expression trend. Magnesium-deficiency-induced lignification, enlargement and cracking in veins of lower leaves might be related to the following factors: (i) numerous transciption factors and genes involved in lignin biosynthesis pathways, regulation of cell cycle and cell wall metabolism were upregulated; and (ii) reactive oxygen species, phytohormone and cell wall integrity signalings were activated. Conjoint analysis of proteome and transcriptome indicated that there were 287 and 56 common elements between DEGs and differentially abundant proteins (DAPs) identified in MDVLL vs MSVLL and MDVUL vs MSVUL, respectively, and that among these common elements, the abundances of 198 and 55 DAPs matched well with the transcript levels of the corresponding DEGs in MDVLL vs MSVLL and MDVUL vs MSVUL, respectively, indicating the existence of concordances between protein and transcript levels.

Distribution of Exchangeable Ca and Mg in the Soil Environment of Yunnan Plateau

2013 ◽  
Vol 726-731 ◽  
pp. 85-89
Author(s):  
Ai Guo Wang ◽  
Wen Wang ◽  
Chen Cheng Hu ◽  
Qin Li ◽  
Zhong Jun He
Keyword(s):  
Chinese Herbs ◽  
Nutrient Elements ◽  
Soil Environment ◽  
Mg Deficiency ◽  
Yunnan Plateau ◽  
Exchangeable Ca ◽  
Essential Nutrient ◽  
Yunnan Baiyao ◽  
Mg Content ◽  
Base Soil

Ca and Mg in soil are essential nutrient elements for plants, and their exchangeable state is mainly effective. This paper studied the characteristic of statistics, distribution and exchangeable Ca/Mg for Chinese herbs plantation base soil of Yunnan Baiyao Group Co.LTD. The results of 214 soil samplings data show that 93.55% soil samplings of exchangeable Ca content were above 800 mg/kg, so the Baiyao base soil was rich in Ca element. 79.44% of the samples were seriously potential Mg deficiency. The moderate exchangeable Ca/Mg (between 5 and 10) accounts for 41.1%. Baiyao base soil with higher exchangeable Ca and Mg mainly distributed in southwest and north parts, and exchangeable Mg content in the southeast corner was also higher.

scholarly journals Magnesium Deficiency Induced Global Transcriptome Change in Citrus sinensis Leaves Revealed by RNA-Seq

2019 ◽  
Vol 20 (13) ◽  
pp. 3129 ◽  
Author(s):  
Yang ◽  
Zhou ◽  
Wang ◽  
Wu ◽  
Ye ◽  
...  
Keyword(s):  
Citrus Sinensis ◽  
Molecular Mechanisms ◽  
Magnesium Deficiency ◽  
Enrichment Analysis ◽  
Co2 Assimilation ◽  
Soluble Carbohydrates ◽  
H2o2 Production ◽  
Rna Seq ◽  
Mg Deficiency ◽  
Real Time Quantitative Pcr

Magnesium (Mg) deficiency is one of the major constraining factors that limit the yield and quality of agricultural products. Uniform seedlings of the Citrus sinensis were irrigated with Mg deficient (0 mM MgSO4) and Mg sufficient (1 mM MgSO4) nutrient solutions for 16 weeks. CO2 assimilation, starch, soluble carbohydrates, TBARS content and H2O2 production were measured. Transcriptomic analysis of C. sinensis leaves was performed by Illumina sequencing. Our results showed that Mg deficiency decreased CO2 assimilation, but increased starch, sucrose, TBARS content and H2O2 production in C. sinensis leaves. A total of 4864 genes showed differential expression in response to Mg deficiency revealed by RNA-Seq and the transcriptomic data were further validated by real-time quantitative PCR (RT-qPCR). Gene ontology (GO) enrichment analysis indicated that the mechanisms underlying Mg deficiency tolerance in C. sinensis may be attributed to the following aspects: a) enhanced microtubule-based movement and cell cycle regulation; b) elevated signal transduction in response to biotic and abiotic stimuli; c) alteration of biological processes by tightly controlling phosphorylation especially protein phosphorylation; d) down-regulation of light harvesting and photosynthesis due to the accumulation of carbohydrates; e) up-regulation of cell wall remodeling and antioxidant system. Our results provide a comprehensive insight into the transcriptomic profile of key components involved in the Mg deficiency tolerance in C. sinensis and enrich our understanding of the molecular mechanisms by which plants adapted to a Mg deficient condition.

Magnesium in tropical and subtropical soils from north-eastern Australia. I. Magnesium fractions and interrelationships with soil properties

Soil Research ◽  
1997 ◽  
Vol 35 (3) ◽  
pp. 615 ◽  
Author(s):  
K. J. Hailes ◽  
R. L. Aitken ◽  
N. W. Menzies
Keyword(s):  
Organic Matter ◽  
Organic Carbon ◽  
Plant Growth ◽  
Clay Content ◽  
Soil Types ◽  
Mg Deficiency ◽  
Eastern Australia ◽  
North Eastern ◽  
Subtropical Soils ◽  
Mg Content

The magnesium (Mg) status of 52 highly weathered, predominantly acidic, surface soils from tropical and subtropical north-eastern Australia was evaluated in a laboratory study. Soils were selected to represent a range of soil types and management histories. Exchangeable Mg concentrations were generally low (median value 0·37 cmol(+)/kg), with deficient levels (<0·3 cmol(+)/kg) being measured in 22 of the soils, highlighting the potential for Mg deficiency as a limitation to plant growth in the region. Furthermore, acid-extractable Mg concentrations, considered a reserve of potentially available Mg, were generally modest (mean and median values, 1·6 and 0·40 cmol(+)/kg, respectively). The total Mg content of the soils studied ranged from 123 to 7894 mg/kg, the majority present in the mineral pool (mean 71%), with smaller proportions in the acid-soluble (mean 11%) and exchangeable (mean 17%) pools, and a negligible amount associated with organic matter (mean 1%). A range of extractant solutions used to displace exchangeable Mg was compared, and found to yield similar results on soils with exchangeable Mg <4 cmol(+)/kg. However, at higher exchangeable Mg concentrations, dilute extractants (0·01 M CaCl2, 0·0125 BaCl2) displaced less Mg than concentrated extractants (1 M NH4Cl, 1 M NH4OAc, 1 M KCl). The concentrated extractants displaced similar amounts of Mg, thus the choice of extractant is not critical, provided the displacing cation is sufficiently concentrated. Exchangeable Mg was not significantly correlated to organic carbon (P > 0·05), and only 45% of the variation in exchangeable Mg could be explained by a combination of pHw and clay content.

Network Analysis Reveals Dysregulated Functional Patterns in Type II Diabetic Skin

2021 ◽  
Author(s):  
Chunan Liu ◽  
Sudha Ram ◽  
Bonnie L. Hurwitz
Keyword(s):  
Network Analysis ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Tissue Expression ◽  
Type Ii ◽  
Skin Disorders ◽  
Long Term Effects ◽  
High Blood Glucose ◽  
Keratinocyte Proliferation ◽  
And Migration

Abstract Skin disorders are one of the most common complications of type II diabetes (T2DM). Long-term effects of high blood glucose leave individuals with T2DM more susceptible to cutaneous diseases, but its underlying molecular mechanisms are unclear. Network-based methods consider the complex interactions between genes which can complement the analysis of single genes in previous research. Here, we use network analysis and topological properties to systematically investigate dysregulated gene co-expression patterns in type II diabetic skin with skin samples from the Genotype-Tissue Expression database. Our final network consisted of 8,812 genes from 73 subjects with T2DM and 147 non-T2DM subjects matched for age, sex, and race. Two gene modules significantly related to T2DM were functionally enriched in the pathway lipid metabolism, activated by PPARA and SREBF (SREBP). Transcription factors KLF10, KLF4, SP1, and microRNA-21 were predicted to be important regulators of gene expression in these modules. Intramodular analysis and betweenness centrality identified NCOA6 as the hub gene while KHSRP and SIN3B are key coordinators that influence molecular activities differently between T2DM and non-T2DM populations. We built a TF-miRNA-mRNA regulatory network to reveal the novel mechanism (miR-21-PPARA-NCOA6) of dysregulated keratinocyte proliferation, differentiation, and migration in diabetic skin, which may provide new insights into the susceptibility of skin disorders in T2DM patients. Hub genes and key coordinators may serve as therapeutic targets to improve diabetic skincare.

scholarly journals Circular RNA AFF4 modulates osteogenic differentiation in BM-MSCs by activating SMAD1/5 pathway through miR-135a-5p/FNDC5/Irisin axis

2021 ◽  
Vol 12 (7) ◽  
Author(s):  
Chao Liu ◽  
An-Song Liu ◽  
Da Zhong ◽  
Cheng-Gong Wang ◽  
Mi Yu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Regulatory System ◽  
Circular Rna ◽  
Luciferase Reporter ◽  
Integrin Αv

AbstractBone marrow-derived mesenchymal stem cells (BM-MSCs), the common progenitor cells of adipocytes and osteoblasts, have been recognized as the key mediator during bone formation. Herein, our study aim to investigate molecular mechanisms underlying circular RNA (circRNA) AFF4 (circ_AFF4)-regulated BM-MSCs osteogenesis. BM-MSCs were characterized by FACS, ARS, and ALP staining. Expression patterns of circ_AFF4, miR-135a-5p, FNDC5/Irisin, SMAD1/5, and osteogenesis markers, including ALP, BMP4, RUNX2, Spp1, and Colla1 were detected by qRT-PCR, western blot, or immunofluorescence staining, respectively. Interactions between circ_AFF4 and miR-135a-5p, FNDC5, and miR-135a-5p were analyzed using web tools including TargetScan, miRanda, and miRDB, and further confirmed by luciferase reporter assay and RNA pull-down. Complex formation between Irisin and Integrin αV was verified by Co-immunoprecipitation. To further verify the functional role of circ_AFF4 in vivo during bone formation, we conducted animal experiments harboring circ_AFF4 knockdown, and born samples were evaluated by immunohistochemistry, hematoxylin and eosin, and Masson staining. Circ_AFF4 was upregulated upon osteogenic differentiation induction in BM-MSCs, and miR-135a-5p expression declined as differentiation proceeds. Circ_AFF4 knockdown significantly inhibited osteogenesis potential in BM-MSCs. Circ_AFF4 stimulated FNDC5/Irisin expression through complementary binding to its downstream target molecule miR-135a-5p. Irisin formed an intermolecular complex with Integrin αV and activated the SMAD1/5 pathway during osteogenic differentiation. Our work revealed that circ_AFF4, acting as a sponge of miR-135a-5p, triggers the promotion of FNDC5/Irisin via activating the SMAD1/5 pathway to induce osteogenic differentiation in BM-MSCs. These findings gained a deeper insight into the circRNA-miRNA regulatory system in the bone marrow microenvironment and may improve our understanding of bone formation-related diseases at physiological and pathological levels.

scholarly journals Interrogating Plant-Microbe Interactions with Chemical Tools: Click Chemistry Reagents for Metabolic Labeling and Activity-Based Probes

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 243
Author(s):  
Vivian S. Lin
Keyword(s):  
Plant Growth ◽  
Immune Responses ◽  
Click Chemistry ◽  
Chemical Biology ◽  
Molecular Mechanisms ◽  
Metabolic Labeling ◽  
Beneficial Microbes ◽  
Plant Hosts ◽  
Microbe Interactions ◽  
Growth Metabolism

Continued expansion of the chemical biology toolbox presents many new and diverse opportunities to interrogate the fundamental molecular mechanisms driving complex plant–microbe interactions. This review will examine metabolic labeling with click chemistry reagents and activity-based probes for investigating the impacts of plant-associated microbes on plant growth, metabolism, and immune responses. While the majority of the studies reviewed here used chemical biology approaches to examine the effects of pathogens on plants, chemical biology will also be invaluable in future efforts to investigate mutualistic associations between beneficial microbes and their plant hosts.

scholarly journals Poplar protease inhibitor expression differs in an herbivore specific manner

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Franziska Eberl ◽  
Thomas Fabisch ◽  
Katrin Luck ◽  
Tobias G. Köllner ◽  
Heiko Vogel ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Transcriptional Level ◽  
Species Identity ◽  
Defense Proteins ◽  
Woody Perennials ◽  
Cdna Sequences ◽  
Black Poplar ◽  
Leaf Area Loss

Abstract Background Protease inhibitors are defense proteins widely distributed in the plant kingdom. By reducing the activity of digestive enzymes in insect guts, they reduce the availability of nutrients and thus impair the growth and development of the attacking herbivore. One well-characterized class of protease inhibitors are Kunitz-type trypsin inhibitors (KTIs), which have been described in various plant species, including Populus spp. Long-lived woody perennials like poplar trees encounter a huge diversity of herbivores, but the specificity of tree defenses towards different herbivore species is hardly studied. We therefore aimed to investigate the induction of KTIs in black poplar (P. nigra) leaves upon herbivory by three different chewing herbivores, Lymantria dispar and Amata mogadorensis caterpillars, and Phratora vulgatissima beetles. Results We identified and generated full-length cDNA sequences of 17 KTIs that are upregulated upon herbivory in black poplar leaves, and analyzed the expression patterns of the eight most up-regulated KTIs via qRT-PCR. We found that beetles elicited higher transcriptional induction of KTIs than caterpillars, and that both caterpillar species induced similar KTI expression levels. Furthermore, KTI expression strongly correlated with the trypsin-inhibiting activity in the herbivore-damaged leaves, but was not dependent on damage severity, i.e. leaf area loss, for most of the genes. Conclusions We conclude that the induction of KTIs in black poplar is controlled at the transcriptional level in a threshold-based manner and is strongly influenced by the species identity of the herbivore. However, the underlying molecular mechanisms and ecological consequences of these patterns remain to be investigated.

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