scholarly journals Olive Biophenols Reduces Alzheimer’s Pathology in SH-SY5Y Cells and APPswe Mice

2018 ◽  
Vol 20 (1) ◽  
pp. 125 ◽  
Author(s):  
Syed Omar ◽  
Christopher Scott ◽  
Adam Hamlin ◽  
Hassan Obied
Keyword(s):  
Morphological Changes ◽  
Low Cost ◽  
Senile Plaques ◽  
Control Diet ◽  
Amyloid Plaque ◽  
Plaque Burden ◽  
Leaf Extracts ◽  
Plaque Deposition ◽  
Candidate Drug ◽  
Copper Zinc

Alzheimer’s disease (AD) is a major neurodegenerative disease, associated with the hallmark proteinacious constituent called amyloid beta (Aβ) of senile plaques. Moreover, it is already established that metals (particularly copper, zinc and iron) have a key role in the pathogenesis of AD. In order to reduce the Aβ plaque burden and overcome the side effects from the synthetic inhibitors, the current study was designed to focus on direct inhibition of with or without metal-induced Aβ fibril formation and aggregation by using olive biophenols. Exposure of neuroblastoma (SH-SY5Y) cells with Aβ42 resulted in decrease of cell viability and morphological changes might be due to severe increase in the reactive oxygen species (ROS). The pre-treated SH-SY5Y cells with olive biophenols were able to attenuate cell death caused by Aβ42, copper- Aβ42, and [laevodihydroxyphenylalanine (l-DOPA)] l-DOPA-Aβ42-induced toxicity after 24 h of treatment. Oleuropein, verbascoside and rutin were the major anti-amyloidogenic compounds. Transgenic mice (APPswe/PS1dE9) received 50 mg/kg of oleuropein containing olive leaf extracts (OLE) or control diet from 7 to 23 weeks of age. Treatment mice (OLE) were showed significantly reduced amyloid plaque deposition (p < 0.001) in cortex and hippocampus as compared to control mice. Our findings provide a basis for considering natural and low cost biophenols from olive as a promising candidate drug against AD. Further studies warrant to validate and determine the anti-amyloid mechanism, bioavailability as well as permeability of olive biophenols against blood brain barrier in AD.

Traumatic Injury Reduces Amyloid Plaque Burden in the Transgenic 5xFAD Alzheimer’s Mouse Spinal Cord

2020 ◽  
Vol 77 (3) ◽  
pp. 1315-1330
Author(s):  
Tak-Ho Chu ◽  
Karen Cummins ◽  
Peter K. Stys
Keyword(s):  
Spinal Cord ◽  
Axonal Injury ◽  
Amyloid Β ◽  
Amyloid Plaque ◽  
Amyloid Deposition ◽  
Plaque Burden ◽  
Wild Type ◽  
Plaque Deposition ◽  
5Xfad Mice ◽  
The Impact

Background: Axonal injury has been implicated in the development of amyloid-β in experimental brain injuries and clinical cases. The anatomy of the spinal cord provides a tractable model for examining the effects of trauma on amyloid deposition. Objective: Our goal was to examine the effects of axonal injury on plaque formation and clearance using wild type and 5xFAD transgenic Alzheimer’s disease mice. Methods: We contused the spinal cord at the T12 spinal level at 10 weeks, an age at which no amyloid plaques spontaneously accumulate in 5xFAD mice. We then explored plaque clearance by impacting spinal cords in 27-week-old 5xFAD mice where amyloid deposition is already well established. We also examined the cellular expression of one of the most prominent amyloid-β degradation enzymes, neprilysin, at the lesion site. Results: No plaques were found in wild type animals at any time points examined. Injury in 5xFAD prevented plaque deposition rostral and caudal to the lesion when the cords were examined at 2 and 4 months after the impact, whereas age-matched naïve 5xFAD mice showed extensive amyloid plaque deposition. A massive reduction in the number of plaques around the lesion was found as early as 7 days after the impact, preceded by neprilysin upregulation in astrocytes at 3 days after injury. At 7 days after injury, the majority of amyloid was found inside microglia/macrophages. Conclusion: These observations suggest that the efficient amyloid clearance after injury in the cord may be driven by the orchestrated efforts of astroglial and immune cells.

scholarly journals Using seed respiration as a tool for calculating optimal soaking times for ‘on-farm’ seed priming of barley (Hordeum vulgare)

2021 ◽  
pp. 1-9
Author(s):  
Javier Carrillo-Reche ◽  
Adrian C. Newton ◽  
Richard S. Quilliam
Keyword(s):  
Morphological Changes ◽  
Low Cost ◽  
Germination Rate ◽  
Seed Priming ◽  
Embryonic Axes ◽  
Seedling Vigour ◽  
Soaking Time ◽  
On Farm ◽  
Seed Respiration

Abstract A low-cost technique named ‘on-farm’ seed priming is increasingly being recognized as an effective approach to maximize crop establishment. It consists of anaerobically soaking seeds in water before sowing resulting in rapid and uniform germination, and enhanced seedling vigour. The extent of these benefits depends on the soaking time. The current determination of optimal soaking time by germination assays and mini-plot trials is resource-intensive, as it is species/genotype-specific. This study aimed to determine the potential of the seed respiration rate (an indicator of metabolic activity) and seed morphological changes during barley priming as predictors of the priming benefits and, thus, facilitate the determination of optimal soaking times. A series of germination tests revealed that the germination rate is mostly attributable to the rapid hydration of embryo tissues, as the highest gains in the germination rate occurred before the resumption of respiration. Germination uniformity, however, was not significantly improved until seeds were primed for at least 8 h, that is, after a first respiration burst was initiated. The maximum seedling vigour was attained when the priming was stopped just before the beginning of the differentiation of embryonic axes (20 h) after which vigour began to decrease (‘over-priming’). The onset of embryonic axis elongation was preceded by a second respiration burst, which can be used as a marker for priming optimization. Thus, monitoring of seed respiration provides a rapid and inexpensive alternative to the current practice. The method could be carried out by agricultural institutions to provide recommended optimal soaking times for the common barley varieties within a specific region.

scholarly journals Preparation of Porous Hydroxyapatite Using Cetyl Trimethyl Ammonium Bromide as Surfactant for the Removal of Lead Ions from Aquatic Solutions

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1617
Author(s):  
Silviu-Adrian Predoi ◽  
Carmen Steluta Ciobanu ◽  
Mikael Motelica-Heino ◽  
Mariana Carmen Chifiriuc ◽  
Monica Luminita Badea ◽  
...  
Keyword(s):  
Cetyl Trimethyl Ammonium Bromide ◽  
Morphological Changes ◽  
Low Cost ◽  
Lead Ions ◽  
Ammonium Bromide ◽  
Porous Hydroxyapatite ◽  
X Ray ◽  
Composition And Structure ◽  
Cetyl Trimethyl Ammonium ◽  
Co Precipitation

In the present study, a new low-cost bioceramic nanocomposite based on porous hydroxyapatite (HAp) and cetyl trimethyl ammonium bromide (CTAB) as surfactant was successfully obtained by a simple chemical co-precipitation. The composition and structure of the HAp-CTAB were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), scanning electron microscope (SEM) equipped with an energy dispersive X-ray (EDX) spectrometer, and N2 adsorption/desorption analysis. The capacity of HAp-CTAB nanocomposites to remove the lead ions from aqueous solutions was studied by adsorption batch experiments and proved by Langmuir and Freundlich models. The Pb2+ removal efficiency of HAp-CTAB biocomposite was also confirmed by non-destructive ultrasound studies. The cytotoxicity assays showed that the HAp-CTAB nanocomposites did not induce any significant morphological changes of HeLa cells after 24 h of incubation or other toxic effects. Taken together, our results suggests that the obtained porous HAp-CTAB powder could be used for the decontamination of water polluted with heavy metals, such as Pb2+.

Laser microdissection reveals regional and cellular differences in GFAP mRNA upregulation following brain injury, axonal denervation, and amyloid plaque deposition

Glia ◽  
2004 ◽  
Vol 48 (1) ◽  
pp. 76-84 ◽  
Author(s):  
Guido J. Burbach ◽  
Doris Dehn ◽  
Domenico Del Turco ◽  
Matthias Staufenbiel ◽  
Thomas Deller
Keyword(s):  
Brain Injury ◽  
Laser Microdissection ◽  
Amyloid Plaque ◽  
Plaque Deposition

scholarly journals Testing an “IoT” Tide Gauge Network for Coastal Monitoring

IoT ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 17-32
Author(s):  
Philip Knight ◽  
Cai Bird ◽  
Alex Sinclair ◽  
Jonathan Higham ◽  
Andy Plater
Keyword(s):  
Sea Level ◽  
Morphological Changes ◽  
Tide Gauge ◽  
Low Cost ◽  
Pressure Sensors ◽  
Wave Activity ◽  
Tide Gauges ◽  
Coastal Monitoring ◽  
Level Data ◽  
Operational Constraints

A low-cost “Internet of Things” (IoT) tide gauge network was developed to provide real-time and “delayed mode” sea-level data to support monitoring of spatial and temporal coastal morphological changes. It is based on the Arduino Sigfox MKR 1200 micro-controller platform with a Measurement Specialties pressure sensor (MS5837). Experiments at two sites colocated with established tide gauges show that these inexpensive pressure sensors can make accurate sea-level measurements. While these pressure sensors are capable of ~1 cm accuracy, as with other comparable gauges, the effect of significant wave activity can distort the overall sea-level measurements. Various off-the-shelf hardware and software configurations were tested to provide complementary data as part of a localized network and to overcome operational constraints, such as lack of suitable infrastructure for mounting the tide gauges and for exposed beach locations.

scholarly journals α-1-Antichymotrypsin Promotes β-Sheet Amyloid Plaque Deposition in a Transgenic Mouse Model of Alzheimer's Disease

2001 ◽  
Vol 21 (5) ◽  
pp. 1444-1451 ◽  
Author(s):  
Lars N. G. Nilsson ◽  
Kelly R. Bales ◽  
Giovanni DiCarlo ◽  
Marcia N. Gordon ◽  
Dave Morgan ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Amyloid Plaque ◽  
Transgenic Mouse Model ◽  
Plaque Deposition ◽  
Model Of Alzheimer’S Disease ◽  
Β Sheet

Targeted Intraparenchymal Delivery of Human NGF by Gene Transfer to the Primate Basal Forebrain for 3 Months Does Not Accelerate β-Amyloid Plaque Deposition

1998 ◽  
Vol 154 (2) ◽  
pp. 573-582 ◽  
Author(s):  
Mark H. Tuszynski ◽  
David E. Smith ◽  
Jeffrey Roberts ◽  
Heather McKay ◽  
Elliott Mufson
Keyword(s):  
Gene Transfer ◽  
Basal Forebrain ◽  
Amyloid Plaque ◽  
Plaque Deposition ◽  
Β Amyloid
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