scholarly journals Royal Jelly Abrogates Cadmium-Induced Oxidative Challenge in Mouse Testes: Involvement of the Nrf2 Pathway

2018 ◽  
Vol 19 (12) ◽  
pp. 3979 ◽  
Author(s):  
Rafa Almeer ◽  
Doaa Soliman ◽  
Rami Kassab ◽  
Gadah AlBasher ◽  
Saud Alarifi ◽  
...  
Keyword(s):  
Royal Jelly ◽  
Histopathological Examination ◽  
Physiological Saline ◽  
Serum Testosterone ◽  
Testicular Tissue ◽  
Redox Status ◽  
Nuclear Antigen ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Oxidative Challenge

The current study examined the efficacy of royal jelly (RJ) against cadmium chloride (CdCl2)-induced testicular dysfunction. A total of 28 Swiss male mice were allocated into four groups (n = 7), and are listed as follows: (1) the control group, who was intraperitoneally injected with physiological saline (0.9% NaCl) for 7 days; (2) the RJ group, who was orally supplemented with RJ (85 mg/kg daily equivalent to 250 mg crude RJ) for 7 days; (3) the CdCl2 group, who was intraperitoneally injected with 6.5 mg/kg for 7 days; and (4) the fourth group, who was supplemented with RJ 1 h before CdCl2 injection for 7 days. Cd-intoxicated mice exhibited a decrease in serum testosterone, luteinizing hormone (LH), and follicle stimulating hormone (FSH) levels. A disturbance in the redox status in the testicular tissue was recorded, as presented by the increase in lipid peroxidation and nitrate/nitrite levels and glutathione (GSH) depletion. Moreover, the activities of glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), and nuclear factor (erythroid-derived 2)-like-2 factor (Nrf2) and their gene expression were inhibited. In addition, interleukin-1ß (IL-1β) and tumor necrosis factor-α (TNF-α) levels were elevated. Furthermore, Cd triggered an apoptotic cascade via upregulation of caspase-3 and Bax and downregulation of Bcl-2. Histopathological examination showed degenerative changes in spermatogenic cells, detachment of the spermatogenic epithelium from the basement membrane, and vacuolated seminiferous tubules. Decreased cell proliferation was reflected by a decrease in proliferating cell nuclear antigen (PCNA) expression. Interestingly, RJ supplementation markedly minimized the biochemical and molecular histopathological changes in testes tissue in response to Cd exposure. The beneficial effects of RJ could be attributed to its antioxidative properties.

Effect of formaldehyde inhalation on Hsp70 in seminiferous tubules of rat testes: an immunohistochemical study

2005 ◽  
Vol 21 (9) ◽  
pp. 249-254 ◽  
Author(s):  
Oğuz Aslan Özen ◽  
Nusret Akpolat ◽  
Ahmet Songur ◽  
İlter Kuş ◽  
İsmail Zararsiz ◽  
...  
Keyword(s):  
Serum Testosterone ◽  
Underlying Mechanism ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Chemical Toxicity ◽  
Testosterone Levels ◽  
Hsp70 Synthesis ◽  
Insight Into ◽  
Formaldehyde Inhalation

One parameter which might provide an insight into the underlying mechanism of the effect of formaldehyde (FA) inhalation on testicular tissue, is the assessment of heat shock protein 70 (Hsp70), which increases promptly in cells exposed to stress caused by chemical toxicity. Thus, following subchronic exposure at cytotoxic concentrations, we studied the immunohistochemical effect of FA inhalation on changes in Hsp70 content in testicular tissue. We used 18 albino Wistar rats divided into three groups, exposed to 0 (control), 5 and 10 ppm FA gas for a total of 91 days, 8 h/day, five days a week. Serum testosterone levels were determined using a chemiluminescent enzyme immunoassay. Testicular tissues were stained with Hematoxylin-Eosine and Hsp70 immunohistochemically performed. Diameters of seminiferous tubules and serum testosterone levels in animals inhaling FA were significantly decreased. In seminiferous epithelium stained for Hsp70, compared to those in the control group, the spermatogenetic cells in the experimental groups demonstrated an obvious increase in immunoreaction spermatides in the adluminal region and especially in the cytoplasm of spermatocytes. Immunoreaction of Hsp70 was detected in the spermatogonias of animals exposed to FA inhalation as opposed to those of the control group. Compared to the control, there was a significant increase in the immunoreactions observed not only in the cytoplasm of primary spermatocytes, but also spermatides in the adluminal region of the seminiferous tubules. In conclusion, FA gas may damage spermatogenetic cells and increase Hsp70 synthesis.

AMELIORATIVE EFFECTS OF MGS04 ON DEXAMETHASONE- INDUCED HISTO-CHEMICAL ALTERATIONS IN THE TESTES OF ALBINO RATS

1969 ◽  
Vol 6 (1) ◽  
pp. 753-757
Author(s):  
INAYATULLAH ◽  
MOHAMMAD KHAN ◽  
MARIYA HIDAYAT ◽  
LUTFUR-RAHMAN ◽  
ASMA SIDDIQUI ◽  
...  
Keyword(s):  
Testosterone Level ◽  
Serum Testosterone ◽  
Concomitant Administration ◽  
Testicular Tissue ◽  
Serum Testosterone Level ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats ◽  
Male Adult ◽  
Group B

BACKGROUND: In experimental animal, dexamethasone-induced impaired spermatogenesis causesdisruption of the normal architecture of seminiferous tubules and alteration in male sexual hormonetestosterone. Concomitant administration of MgS04 preserved the cytoarchitecture of testes as well ashormonal regulation in albino rats.OBJECTIVE: This study was designed to observe the ameliorative effects of MgS04 on the histologyof testes and there correlation with serum testosterone level during dexamethasone administration inalbino rats.METHODOLOGY: This experimental study was conducted in the department of Anatomy, BasicA LMedical Sciences Institute (BMSI), Jinnah Postgraduate Medical Centre (JPMC), Karachi from 5 Aprilto 25th April 2012. Thirty healthy male adult albino rats were included in the study and divided equallyinto 3 groups. Each group comprising 10 animals. Group-A served as Control. Group-B receivedDexamethasone (intraperitonealy) at the dose of 4mg/kg body weight/24 hours. Group-C receivedDexamethasone at the same dose as in group-B and additionally given MgS04 (intramuscularly) at thedose of 20mg/kg/24hours.RESULTS: MgS04 significantly preserved the cytoarchitecture of testes, as well as minimizedalteration in serum testosterone level in group-C animals.CONCLUSION: MgSO4 has restored both the histological and biochemical damaging effects inducedby dexamethasone in Rats testes.KEYWORDS: Dexamethasone, MgS04, Testicular tissue, Testosterone.

Consequences of Microwave oven Exposed Diet on The Basal Lamina of Seminiferous Tubules of Mice

2021 ◽  
Vol 15 (8) ◽  
pp. 2141-2144
Author(s):  
Kishwar Naheed ◽  
Muhammad Saad Abdullah ◽  
Maria Yousaf ◽  
Humaira Ali ◽  
Fareeha Mushtaq ◽  
...  
Keyword(s):  
Basal Lamina ◽  
Treated Group ◽  
Testicular Tissue ◽  
Microwave Oven ◽  
Mentha Piperita ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Protective Effects ◽  
Trial Methodology ◽  
Experimental Group

Usage of electronic gadgets like microwave oven is increasing day by day that heats the food by exposing it to electromagnetic radiations which has many hazardous effects on human health including fertility. Aim: To find the effects of microwave oven exposed diet on basal lamina of seminiferous tubules of mice alongwith protective effects of Mentha piperita and melatonin on the same tissue. Study Design: Randomized control trial. Methodology: Adult male mice (n=32) were divided into four groups. Control group (G1) received standard pellets prepared for mice. Second group (G2) was given mice pellets exposed to microwave oven. Third group (G3) received Mentha Piperita leaf extract along with mice pellets exposed to microwave oven and the fourth group (G4) received oral melatonin along with pellets exposed to microwave oven. Later their testicular tissue was removed for histological examination while basal lamina disruption was assessed by scoring. Data analyzed by SPSS 22.0v. Results: In group G2, there was slight disruption in the basal lamina in 75% of the cases while in experimental group G3, there was slight disruption of basal lamina only in 12.5% of the cases. However, in group G4, only 25% specimen had slight disruption of basal lamina Conclusion: It was concluded that microwave oven exposed diet produced severe disruption of basal lamina in group G2 that decreased in Mentha piperita and melatonin treated groups. However, Mentha piperita treated group produced better results than melatonin treated group. Keywords: Mice, Testis, Basal Lamina, Mentha piperita and Melatonin

INVESTIGATION OF THE EFFECT OF BLACK SEED OIL ON DIABETES-INDUCED - TESTICULAR DAMAGE: A HISTOPATHOLOGICAL STUDY

2021 ◽  
pp. 29-31
Author(s):  
Seval Kaya ◽  
Yusuf Nergiz ◽  
Firat Asir
Keyword(s):  
Tissue Damage ◽  
Seed Oil ◽  
Histopathological Examination ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Histopathological Study ◽  
Diabetes Group ◽  
Black Seed ◽  
Black Seed Oil

In this study, it was aimed to investigate the protective effect of black seed oil against testicular tissue damage in diabetic rats. A total of 18 male rats were divided into 3 groups, including 6 rats in each group.Groups; control (n=6), diabetes (n=6), diabetes + black seed oil (n=6). A single dose of 45 mg / kg streptozocine (STZ) was injected intraperitoneally to induce diabetes. Diabetes + Black seed oil group: For 56 days, 2.5 ml / kg of black seed oil was administered orally to rats.The rats were sacriced at the end of 56 days. Testicular tissues were taken for routine parafn tissue processing for histopathological examination. Parafn sections were stained with Hematoxylin-Eosin and PAS and examined under a light microscope. Atrophy and degeneration were observed in the seminiferous tubules of diabetic group. Histology of black seed oil group sections were similar to that of control group. A signicant difference was found between the black seed oil group and the diabetes group in terms of blood glucose values. As a result, we think that Black Seed Oil ameliorates to the tissue damage caused by diabetes and the decrease in blood sugar value.

scholarly journals Azathioprine and Methotrexate impaired the morphology and functions of the testes in adult wistar rats

2014 ◽  
Vol 31 (02) ◽  
pp. 075-081
Author(s):  
A. Akinlolu ◽  
O. Akinola ◽  
P. Khobe ◽  
K. Obasi ◽  
O. Dada
Keyword(s):  
Adult Male ◽  
Wistar Rats ◽  
Follicle Stimulating Hormone ◽  
Physiological Saline ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Connective Tissues ◽  
Group I ◽  
Male Wistar Rats ◽  
Dose Dependent

Abstract Introduction: AAzathioprine and Methotrexate are both used in the treatment of cancer; and are classified as cytotoxic drugs with reported adverse effects such as oxidative damage to the DNA/RNA, the testes and sperm cells. This study, therefore, tested the hypothesis that AAzathioprine and Methotrexate administrations impair the morphology and functions of the testes in adult male wistar rats. Methods: AAzathioprine (50-150mg per day) and Methotrexate (2.5mg per week) are used in the treatment of cancer in adult Man. We tested the hypothesis that AAzathioprine and Methotrexate impair the morphology and functions of testes in rats. Forty adult male wistar rats (150-230g) were employed in the study: Control Group I received physiological saline while Experimental Groups II - V received oral administrations of 5mg/kg/bodyweight of AAzathioprine per day, 15mg/kg/bodyweight of AAzathioprine per day, 8mg/kg/bodyweight of Methotrexate per week and 20mg/kg/bodyweight of Methotrexate per week respectively for 35 days. Results: Histological examinations of the testes of rats of Groups II - V showed dose-dependent morphological anomalies such as fewer collagen ibers of connective tissues, disrupted seminiferous tubules and scanty spermatozoa when compared to rats of Group I. Statistical analyses showed dose-dependent elevated levels (P≤0.05) of superoxide dismutase and malondialdehyde in testes homogenates of rats of Groups II - V when compared to rats of Group I. This implied increased oxidative stress in rats of Groups II - V. Evaluations of Follicle Stimulating Hormone and Testosterone showed dose-dependent significantly elevated levels (P≤0.05) in rats of Groups II - V when compared to rats of Group I. Conclusions: Our findings are consistent with the stated hypothesis.

scholarly journals The Effects of Vasectomy on Testicular Tissue of Mice: Histological Changes and DNA Fragmentation Study

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Salman MO ◽  
Al-Wasiti EA ◽  
Thamir KA ◽  
Al-Ani IM ◽  
Al-Salihi AR
Keyword(s):  
Single Cell ◽  
Dna Fragmentation ◽  
Gel Electrophoresis ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Sham Operation ◽  
Male Mice ◽  
Histological Changes ◽  
Single Cell Gel Electrophoresis

Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the testes were evaluated for histological changes and DNA fragmentation by single cell gel electrophoresis (comet assay). Results: Marked alterations were observed in the testes of vasectomized mice, including degeneration of spermatids, thickened basement membrane, dilatation of the seminiferous tubules, exfoliation of germ cells, reduction in the seminiferous cell population, vacuolated appearance of the epithelium in the tubules and marked interstitial fibrosis. Single cell gel electrophoresis showed a highly significant (P<0.0001) increase in DNA damage among vasectomized mice (46.02%) compared with control group (%27.17) after six weeks of operation. Conclusion: Vasectomy induced deterioration in the seminiferous tubules associated with increased testicular cell’s DNA fragmentation.

scholarly journals Testicular Morphological Changes Produced by Fluoroquinolones in Adult Male Albino Rats

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
Rubina Iqbal ◽  
Saud Iqbal ◽  
Iram Atta
Keyword(s):  
Adult Male ◽  
Leydig Cells ◽  
Morphological Changes ◽  
Sperm Count ◽  
Research Work ◽  
Testicular Tissue ◽  
Reproductive Organs ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats

AbstractObjectives:  The objective of this research work was to observe the testicular morphological changes produced by fluoroquinolones in the reproductive organs of adult male albino rats, and to see whether these changes are reversible after discontinuation of the drugs.Materials and Method:  Eighty adult male albino rats weighing 200 – 300 gms were randomly selected and divided into four groups i.e. A, B, C & D, having 20 animals in each group. A, B & C, were the experimental groups & D served as control group. All the groups were further divided into sub groups 1 & 2. Three fluoroquinolones i.e. Ciprofloxacin (135 mg / kg / day), Ofloxacin (75 mg / kg / day) & Enoxacin (12.5 mg / kg/ day) were given to the groups A, B & C respectively for 42 days. Animals of group D received dis-tilled water only. Animals of sub groups A1, B1, C1 &D1 were sacrificed on 42nd day and testicular tissue was obtained for morphological study. Animals of subgroups A2, B2, C2 & D2 were sacrificed on 84th day and testicular tissue for morphological changes was taken. No of leydig cells, height of epithelium and diameter of seminiferous tubules were taken as experimental parameters for morphological changes.Results:  The study indicated statistically significant (P < 0.05) decrease in height of epithelium, diameter of seminiferous tubules and no. of leydig cells in experimental groups as compared to the control groups.Conclusion:  The changes observed in morphology could lead to decrease in sperm count and testosterone levels. This study suggests gonadotoxic potentials of fluoroquinolones and adds concern to the indiscriminate and widespread use of fluoroquinolones and recommends more rational use of these drugs.

scholarly journals Allopurinol and Febuxostat Equally Attenuate Methotrexate-Induced Testicular Injury In Rats Via Activation Of EGF/ ERK1/2/HO‐1 Signaling Pathway.

2021 ◽  
Author(s):  
Remon R Rofaeil ◽  
Mohamed A. Ibrahim ◽  
Reham H. Mohyeldin ◽  
Walaa Y. Abdelzaher
Keyword(s):  
Protective Effect ◽  
Histopathological Examination ◽  
Serum Testosterone ◽  
Free Testosterone ◽  
Testicular Tissue ◽  
Testicular Damage ◽  
Purine Analogue ◽  
Tnf Α ◽  
Significant Difference ◽  
Testicular Injury

Abstract Methotrexate (MTX) is commonly used in the management of several malignancies and autoimmune disorders; however, testicular damage is one of the most detrimental effects of MTX administration. In the current study, we evaluated the possible protective effect of xanthine oxidase inhibitors either purine analogue; allopurinol (ALL) or non-purine analogue; febuxostat (FEB) in testicular injury induced by MTX in rats. Gonadotoxicity was induced by a single dose of MTX (20 mg/kg, i.p.). ALL and FEB were administered orally in the following daily doses (100, 10 mg/kg, respectively) for 15 days. Total and free testosterone were measured in serum. In addition, total antioxidant capacity (TAC), epidermal growth factor (EGF), malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), extracellular signal-regulating kinase1/2 (ERK1/2), and nitric oxide (NO) end products were measured in testicular tissues. At the same time, immunoexpression of HO-1in testicular tissue was measured. Histopathological examination was done. ALL and FEB increased total and free serum testosterone. Both drugs showed a significant reduction in testicular MDA, NO, TNF-α levels with an increase in TAC, EGF, and ERK1/2 levels in testicular tissue. Furthermore, both drugs enhanced HO-1 immunoexpression in testicular tissue. All these findings were parallel to the preservation of normal testicular architecture in rats treated with ALL and FEB. In conclusion, All and FEB were protective against testicular damage induced by MTX through anti-inflammatory, anti-apoptotic, and antioxidant actions which might be through activation of the EGF/ERK1/2/HO-1 pathway. At the same time, no significant difference between ALL and FEB was noticed in this protective effect.

scholarly journals EFFECT OF LYCOPENE IN AMELIORATION OF TESTICULAR AND RENAL TOXICITY INDUCED BY BOLDENONE UNDECYLENATE IN MALE ALBINO RATS

2019 ◽  
Vol 3 (3) ◽  
Author(s):  
Samar S Ibrahim ◽  
Alshaimaa M Said
Keyword(s):  
Dna Fragmentation ◽  
Renal Toxicity ◽  
Histopathological Examination ◽  
Kidney Tissue ◽  
Serum Testosterone ◽  
Control Group ◽  
Albino Rats ◽  
Total Antioxidant ◽  
Factor Α ◽  
Necrosis Factor

Background: The present study was designed to evaluate the relative ameliorating efficacy of lycopene against the deleterious effects of boldenone, an androgenic steroid, on the rat testis and kidney.  Materials and Methods: 40 male albino rats were divided into four groups; control group received intramuscular (i.m) injection of olive oil once a week; lycopene (Lc) group received lycopene (10 mg/kg b.w p.o daily); boldenone (Bol) group received (5 mg/kg b.w i.m once a week); Bol + Lc group received boldenone (5 mg/kg b.w i.m once a week) and lycopene (10 mg/kg b.w p.o daily) all for four weeks. Results: intramuscular injection of boldenone significantly induced lipid peroxidation and DNA fragmentation as well as inhibited total antioxidant capacity (TAC) and catalase (CAT) activity in testis and kidney tissue. Additionally, up-regulation of Bax and down-regulation of Bcl-2 gene expression after Bol injection along with marked increase in serum inflammatory cytokines and decrease in serum testosterone. These alterations were confirmed by the histopathological examination of testis and kidney. On the other hand, lycopene oral administration attenuated the testicular and renal injuries induced by boldenone injection. Conclusion: administration of antioxidants as lycopene effectively ameliorated the adverse effects of boldenone on testis and kidney tissues. Key words: Boldenone undecylenate, lycopene, DNA fragmentation, interleukin-1β, tumor necrosis factor-α, apoptosis.

scholarly journals Histological Effects of Titanium Dioxide Nanoparticles Size 10 Nm in Mice Testes

2017 ◽  
Vol 5 (2) ◽  
pp. 158 ◽  
Author(s):  
Imad Taher Abdulla
Keyword(s):  
Titanium Dioxide ◽  
Titanium Dioxide Nanoparticles ◽  
Testicular Tissue ◽  
Germinal Epithelium ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Histological Changes ◽  
Low Concentrations ◽  
High Concentrations ◽  
Histological Effect

In the present study, the histological effect of titanium dioxide nanoparticles (TDN) on testicular tissue of mature Mus musculus mice was investigated. The animals were divided into six groups, control group treated with TDN free saline and five groups treated with TDN as follow 5, 10, 50, 100 and 150 mg/kg B.W. The results showed that TDN has histological effects on testicular tissue like sever congestion, mild edema between seminiferous tubules (STs) and decrease the thickness of germinal epithelium at low concentrations, While, the histological changes at high concentrations involved disturbance in STs diameters, sever edema between STs, sever vaculation in the germinal epithelium and necrosis in spermatogonia, germinal epithelium and Sertoli cells.

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