scholarly journals Nitric Oxide Enhancing Resistance to PEG-Induced Water Deficiency is Associated with the Primary Photosynthesis Reaction in Triticum aestivum L.

2018 ◽  
Vol 19 (9) ◽  
pp. 2819 ◽  
Author(s):  
Ruixin Shao ◽  
Huifang Zheng ◽  
Shuangjie Jia ◽  
Yanping Jiang ◽  
Qinghua Yang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Triticum Aestivum L ◽  
Light Energy ◽  
Phosphorylation Sites ◽  
Water Deficiency ◽  
Primary Reaction ◽  
Wheat Seedlings ◽  
Chl A ◽  
Significant Difference ◽  
Phosphorylated Peptides

Photosynthesis is affected by water-deficiency (WD) stress, and nitric oxide (NO) is a free radical that participates in the photosynthesis process. Previous studies have suggested that NO regulates excitation-energy distribution of photosynthesis under WD stress. Here, quantitative phosphoproteomic profiling was conducted using iTRAQ. Differentially phosphorylated protein species (DEPs) were identified in leaves of NO- or polyethylene glycol (PEG)-treated wheat seedlings (D), and in control seedlings. From 1396 unique phosphoproteins, 2257 unique phosphorylated peptides and 2416 phosphorylation sites were identified. Of these, 96 DEPs displayed significant changes (≥1.50-fold, p < 0.01). These DEPs are involved in photosynthesis, signal transduction, etc. Furthermore, phosphorylation of several DEPs was upregulated by both D and NO treatments, but downregulated only in NO treatment. These differences affected the chlorophyll A–B binding protein, chloroplast post-illumination chlorophyll-fluorescence-increase protein, and SNT7, implying that NO indirectly regulated the absorption and transport of light energy in photosynthesis in response to WD stress. The significant difference of chlorophyll (Chl) content, Chl a fluorescence-transient, photosynthesis index, and trapping and transport of light energy further indicated that exogenous NO under D stress enhanced the primary photosynthesis reaction compared to D treatment. A putative pathway is proposed to elucidate NO regulation of the primary reaction of photosynthesis under WD.

Diluted versus potentitized probes of silver nitrate (10e-2 to 10e-10) and wheat germination

2021 ◽  
Vol 15 (4) ◽  
pp. 22-23
Author(s):  
Corinne Kraus
Keyword(s):  
Silver Nitrate ◽  
Triticum Aestivum L ◽  
Distilled Water ◽  
Special Issue ◽  
High Dilution ◽  
Wheat Seedlings ◽  
Significant Difference ◽  
High Dilutions ◽  
Group A ◽  
Wheat Germination

Corinne Kraus, Ute Knobloch, Scherer Waltraud, Peter Christian Endler Interuniversity College for Health and Development Graz / Castle of Seggau, Austria Background In 1926, an influence of a homeopathically prepared high dilution of silver nitrate on the growth of coleoptiles of wheat seedlings was described (Kolisko 1926). Later, in an extensive series of experiments, wheat was observed under the influence of extremely diluted agitated silver nitrate (10e-23, “24x”). Stalk lengths clearly indicate that development is enhanced by the probe silver nitrate 24x as compared to control (Scherer et al. 2015). A preliminary experiment was performed in early autumn 2015 on stalk growth of wheat seedlings treated with (not potentized) dilutions of silver nitrate 10e-6 to 10e-10 (“6e to 10e”), compared to potentized silver nitrate 6x to 10x (N = 100 per group). A clear, albeit not statistically significant trend was observed of 6x-stalks being longer (23.4 + 16.2 mm) than 6e-stalks (13.0 + 10.9 mm). Objective The aim of this study was to investigate the influence of diluted versus potentized low dilutions of silver nitrate (10e-2 to 10e-10) on wheat germination. Method The experiments were performed in late autumn 2015 on wheat grain (Triticum aestivum L., Capo variety). The grains were observed under the influence of aqueous solutions 10-2 to 10-10 part per weight of silver nitrate, either diluted in steps of 1 : 10 in distilled water by mere pipetting (probes “2e – 10e”), or diluted and agitated in steps of 1:10 (to create potentized probes “2x – 10x”). Untreated distilled water (“w”) served as an additional control. All probes were applied blindly. 100 grains were observed per treatment group in each of the groups resulting a total of 2,000 grains. Grains were placed in glass dishes, probes were added and dishes were covered with lids and placed in drawers . The following endpoint criteria were defined: K1 = visible emergence of sprout material, K2 = lifting of the operculum and emergence of the sprout and W1 = development of three roots.   Result Germination rates K1 of seedlings treated with “w”-probes (blue), with “e”-probes ranging from 2e to 10e (black) and with “x”-probes ranging from 2x to 10x (red) at the measuring points 20h, 24h and 28h (from left to right for each of the probes). In K1, K2 and W, there is an obvious increase of germination rates from the high to the lower concentrations of silver nitrate, both in the “e” and in the “x”-groups and observable at 20h, 24h and 28h (p < 0.01). In contrast, germination rates of the two „w“-probes are practically alike (p > 0,05) When “e” and “x”-data are compared, germination rates are higher under the influence of “x” than under the influence of “e” (p < 0.01 for the pooled “x”-values compared to the pooled “e”-values with regard to K1 as well as K2 as well as W). Conclusion: A significant difference was found between wheat grains treated with mere dilutions compared to grains treated with potentised dilutions. References 1. Endler PC, Belavite P, Bonamin L,Jäger T, Mazon S. Replication of fundamental research models in ultra high dilutions 1994 and 2015 – update on a bibliometric study. Special issue Homeopathy London. 2015 a Oktober;104(4):234-45. 2. Endler PC, Schulte J, Stock-Schroeer B, Stephen S. Ultra high Dilution 1994 revisited 2015 – the state of follow-up research. Special issue Homeopathy London. 2015 b Oktober;104(4):223-6. 3. Kolisko L. Physiologischer Nachweis der Wirksamkeit kleinster Entitäten bei 7 Metallen – Wirkung von Licht und Pflanzen auf das Pflanzenwachstum. Dornach Schweiz: Philosophisch-Anthroposophischer Verlag am Goetheanum; 1926. 4. Kraus C, Knobloch U. Diluted versus diluted and agitated probes of silver nitrate (10-2 to 10-10) and wheat germination, Thesis (MSc); branch campus UCN at Interuniversity College Graz / Schloss Seggau; 2016. 5. Scherer-Pongratz W., Endler P.C., Lothaller H., Stephen S. Wheat and ultra high diluted silver nitrate – further experiments and re-analysis of data. Special issue Homeopathy London. 2015;104(4):246-9.

Water Relations in Young Growing Wheat Leaves after Application of (2-Chloroethyl)trimethyl- ammoniumchloride (CCC) to the Roots of Wheat Seedlings

1985 ◽  
Vol 40 (9-10) ◽  
pp. 745-747
Author(s):  
Jutta Bode ◽  
Aloysius Wild
Keyword(s):  
Water Relations ◽  
Reducing Sugars ◽  
Triticum Aestivum L ◽  
Water Deficiency ◽  
Pressure Potential ◽  
Tissue Water ◽  
Wheat Seedlings ◽  
Diffusive Resistance ◽  
Wheat Leaves ◽  
Synthetic Growth

Abstract The influence on the water relations of the third developing leaf of (2-chloroethyl)trimethylammoniumchloride, a synthetic growth regulator, applied to the roots of young wheat plants (Triticum aestivum L.) has been investigated. The tissue water potential and the pressure potential were found to be reduced by several bars in comparison to the untreated controls, whereas the osmotic potential remained unchanged. The content of soluble reducing sugars was considerably increased in the cell sap of CCC-treated leaves. With this accumulation, however, the turgor was not maintained. Additionally, CCC-treated leaves showed considerably lower transpiration rates and higher diffusive resistance than the controls. Thus, the application of CCC to the roots causes alterations in the water relations of developing wheat leaves, which resemble those induced by water deficiency.

Nitric Oxide Alleviates Iron Toxicity by Reducing Oxidative Damage and Growth Inhibition in Wheat (Triticum aestivum L.) Seedlings

2019 ◽  
Vol 5 (01) ◽  
pp. 16-22
Author(s):  
Nalini Pandey ◽  
Laxmi Verma
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Oxidative Damage ◽  
Sodium Nitroprusside ◽  
Dry Weight ◽  
Triticum Aestivum L ◽  
Tolerance Index ◽  
Promoting Effect ◽  
Wheat Seedlings ◽  
Protein Thiols

Nitric oxide (NO) is an important bioactive signaling molecule in plants which modulates a variety of physiological processes and responses to abiotic and biotic stresses. In this study, the effects of exogenous NO supplied as sodium nitroprusside (SNP) in wheat seedlings under ironinduced oxidative damage was investigated. An appropriate concentration of NO was determined by conducting a preliminary experiment. In solution culture, wheat seeds were grown in the control (100 μM Fe), and toxic Fe (400 μM Fe) levels and the toxic Fe supply was treated with various levels of (50, 100, 200 and 500 μM) sodium nitroprusside (SNP). The results indicated that 400 μM Fe significantly decreased percentage germination, tolerance index, root lengths as well as fresh and dry weight compared to control. Exogenous SNP attenuated the inhibition of wheat seed germination. The promoting effect was most pronounced at 100 μM SNP. The accumulated concentration of iron and active Fe was significantly decreased by SNP treated Fe toxic seedlings. Toxicity of Fe caused oxidative stress by elevating hydrogen peroxide (H2O2), malondialdehyde (MDA) and proline contents in roots of wheat seedlings. One hundred μM SNP counteracted Fe toxicity by reducing the H2O2, MDA and proline contents of toxic Fe exposed seedlings. Meanwhile, application of SNP markedly reduced the activities of superoxide dismutases (SOD), catalases (CAT), peroxidase (POD), ascorbate peroxidases (APX), non protein thiols (NPT) and of glutathione reductase (GR) and increased ascorbate (ASc) compared with Fe toxic treatment alone, thereby indicating the modulation of the antioxidative capacity in the root under Fe stress by NO. The results indicated that the exogenous application of SNP, improved the antioxidant enzymes activity of wheat seedlings against Fe induced oxidative stress.

scholarly journals First Report of Seedling Blight Caused by Sclerotium rolfsii on Wheat in Oklahoma

Plant Disease ◽  
2006 ◽  
Vol 90 (5) ◽  
pp. 686-686 ◽  
Author(s):  
V. Choppakatla ◽  
R. M. Hunger ◽  
H. A. Melouk
Keyword(s):  
Filter Paper ◽  
Sclerotium Rolfsii ◽  
Significant Risk ◽  
Triticum Aestivum L ◽  
The United States ◽  
Damping Off ◽  
Wheat Seedlings ◽  
First Report ◽  
Significant Difference ◽  
Pure Cultures

Wheat (Triticum aestivum L.) is an important crop in Oklahoma and throughout the Central Plains of the United States. The soilborne fungus, Sclerotium rolfsii, is a major pathogen on peanut (Arachis hypogaea L.) but is not known to cause major damage on wheat. During September of 1998, damping-off and rotting of young wheat seedlings were observed in breeder plots in Payne County, OK. The occurrence of symptoms was sporadic with an estimated stand reduction of 10 to 15%. Symptomatic plants were collected from the field and brought to the laboratory. Sclerotia-like bodies from the symptomatic plants were surface disinfested in aqueous 1% NaOCl for 2 min and allowed to germinate at 25 ± 2°C on sterile filter paper moistened with a 1% aqueous solution of methanol. Aerial mycelia from germinating sclerotia were transferred to potato dextrose agar amended with 100 ppm of streptomycin (SPDA) to obtain pure cultures. Pure cultures had coarse, white mycelium distinctive of S. rolfsii and produced very small (0.05 to 0.1 mm), abundant, round, brown sclerotia on the surface of the medium after 15 days of incubation. Pathogenicity was tested on three hard red winter wheat cultivars commonly grown in Oklahoma (Jagger, 2137, and 2174). Four plants of each cultivar were inoculated at the two-leaf stage (Feekes' scale stage 1) by placing a 0.5-cm agar disk removed from a 3-day-old culture onto a 1-cm diameter filter paper that was then pressed to the base of the shoot. Noninoculated plants were used as a control. After inoculation, pots were covered with polyethylene sheets to maintain 95 to 100% relative humidity and incubated at 25 ± 2°C in the greenhouse. Lesions were initially superficial, yellowish, and water soaked. Lesions expanded and resulted in damping-off of seedlings. Noninoculated plants were free of disease and remained healthy. No significant difference (P ≤ 0.05) in disease severity was observed among the cultivars. To fulfill Koch's postulates, the fungus was reisolated onto SPDA where it had the same characteristics as the initial culture. To our knowledge, this is the first report of S. rolfsii on wheat in Oklahoma. Even though S. rolfsii is not expected to pose a significant risk to wheat production, infection of wheat may enhance survival of S. rolfsii and facilitate infection and losses in a following peanut crop. This is especially important in certain areas of Oklahoma where a wheat-peanut rotation is occasionally practiced.

scholarly journals Seed Priming with Endophytic Bacillus subtilis Modulates Physiological Responses of Two Different Triticum aestivum L. Cultivars under Drought Stress

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1810
Author(s):  
Oksana Lastochkina ◽  
Darya Garshina ◽  
Sergey Ivanov ◽  
Ruslan Yuldashev ◽  
Regina Khafizova ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Bacillus Subtilis ◽  
Drought Stress ◽  
Negative Impact ◽  
Growth Parameters ◽  
Triticum Aestivum L ◽  
Wheat Seedlings ◽  
Chl A ◽  
Drought Conditions ◽  
Germination And Growth

The protective effects against drought stress of the endophytic bacterium Bacillus subtilis 10-4 were measured by studying the priming response in two wheat (Triticum aestivum L.)—Ekada70 (E70) and Salavat Yulaev (SY)—lines, tolerant and susceptible to drought, respectively. B. subtilis 10-4 improved germination and growth parameters under normal conditions in both cultivars with the most pronounced effect observed in cv. E70. Under drought conditions, B. subtilis 10-4 significantly ameliorated the negative impact of stress on germination and growth of cv. E70, but had no protective effect on cv. SY. B. subtilis 10-4 induced an increase in the levels of photosynthetic chlorophyll (Chl) a, Chl b, and carotenoids (Car) in the leaves of cv. E70, both under normal and drought conditions. In cv. SY plants, bacterial inoculation decreased the contents of Chl a, Chl b, and Car under normal conditions, but pigment content were almost recovered under drought stress. B. subtilis 10-4 increased water holding capacity (WHC) of cv. E70 (but did not affect this parameter in cv. SY) and prevented the stress-induced decline in WHC in both cultivars. Notably, B. subtilis 10-4 increased endogenous salicylic acid (SA) concentration in both cultivars, especially in cv. E70. Moreover, B. subtilis 10-4 reduced drought-induced endogenous SA accumulation, which was correlated with the influence of endophyte on growth, indicating a possible involvement of endogenous SA in the implementation of B. subtilis-mediated effects in both cultivars. Overall, B. subtilis 10-4 inoculation was found to increase drought tolerance in seedlings of both cultivars, as evidenced by decreased lipid peroxidation, proline content, and electrolyte leakage from tissues of wheat seedlings primed with B. subtilis 10-4 under drought conditions.

scholarly journals Genotype difference in the physiological characteristics of phosphorus acquisition by wheat seedlings in alkaline soils

2020 ◽  
Vol 66 (No. 10) ◽  
pp. 506-512
Author(s):  
Lianhe Zhang ◽  
Feiyan Yu ◽  
Wei Li ◽  
Xiaokai Gao ◽  
Peng Li ◽  
...  
Keyword(s):  
Triticum Aestivum L ◽  
Physiological Characteristics ◽  
Alkaline Soils ◽  
Wheat Seedlings ◽  
Proton Secretion ◽  
P Acquisition ◽  
P Concentration ◽  
Significant Difference ◽  
Genotype Difference ◽  
The Difference

Phosphorus (P) in soils occurs predominately as insoluble inorganic P and organic P. However, key factors controlling P acquisition by wheat (Triticum aestivum L.) seedlings are unclear. In this study, the difference in the physiological characteristics of P acquisition in alkaline soils was investigated in wheat seedlings of two cultivars Aikang 58 and Zhoumai 22. The results indicated that the shoot P concentration of Aikang 58 was significantly higher than that of Zhoumai 22 when supplied with 0 and 70 kg/ha of pure P under field conditions. When cultured in sterile nutrition solutions with equimolar amounts of P corresponding to KH<sub>2</sub>PO<sub>4</sub>, Ca<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>, and Ca(H<sub>2</sub>PO<sub>4</sub>)<sub>2</sub> for 6 days, the P concentration in the shoots and roots of the seedlings of Aikang 58 was significantly higher than that of Zhoumai 22. However, the P concentration of seedlings of Aikang 58 did not exhibit a significant difference than that of Zhoumai 22 when cultured in phytic acid solution. Further studies suggested that the proton secretion rate was higher, and the root phosphatase activity was significantly lower in Aikang 58 compared with those in Zhoumai 22. After 48 h of successive P starvation, the inorganic phosphate (P<sub>i</sub>) uptake rate of Aikang 58 was significantly higher compared with that of Zhoumai 22. However, no significant differences existed in the root morphology between the two cultivars. Hence, the higher P acquisition in the wheat seedlings of Aikang 58 was attributed to a higher rate of proton secretion and a stronger capacity for P<sub>i</sub> uptake.  

scholarly journals PROTECTION WITH A BIOACTIVE COMPOSITION BASED ON PLUMBAGIN (CERATOSTIGMA PLUMBAGINOIDES BUNGE) AND NA-ASCORBATE PIGMENTS AND PSII IN OXIDATIVE STRESS

2021 ◽  
pp. 8-13
Author(s):  
R. Ganiyeva ◽  
S. Dadashova ◽  
J. Jafarova ◽  
R. Gasanov
Keyword(s):  
Triticum Aestivum L ◽  
Delayed Fluorescence ◽  
Simultaneous Action ◽  
Donor Side ◽  
Wheat Seedlings ◽  
Ps Ii ◽  
Sequential Action ◽  
Chl A ◽  
Acceptor Side ◽  
Corrective Effect

The protective properties of Na-ascorbate and a bioactive composition (BAC) obtained on the basis of plumbagin from roots (Ceratostigma plumbaginoides Bunge) under the toxic effect of Zn2+ and Ni2+ on wheat seedlings (Triticum aestivum L.) were studied. The change in the characteristics of millisecond delayed fluorescence (msec DF Chl a) reflecting the state of PS II shows a decrease in the blocking of the activity of the electron transport chain (ETC) on the acceptor side under the action of Zn2+ and Ni2+ and on the donor side under the action of Ni2+. The resistance of Chl b 650 decreases and the resistance of carotenoids to this stress increases. The action of Na-asс and BAC changes the ratio of pigments. Na-asc restores the activity of the donor side and increases the work of the acceptor side of the ETC upon sequential action with Zn2+. The activity of the acceptor side of the ETC is restored with the simultaneous action of Na-asc with Ni2+. The effect of the BAC is manifested in the restoration of the activity of the donor side of the ETC PS II with sequential action with metals. The corrective effect of Na-asc and LHC is determined by their ability to neutralize reactive oxygen species formed under stress and to support the redox reactions of photosystems.

Effect of 24-epibrassinolide on localization of ABA, AZP, and dehydrins in wheat plant roots during dehydration

Biomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 329-336
Author(s):  
A.R. Lubyanova ◽  
F.M. Shakirova ◽  
M.V. Bezrukova
Keyword(s):  
Triticum Aestivum ◽  
Wheat Germ ◽  
Wheat Plant ◽  
Triticum Aestivum L ◽  
Immunohistochemical Localization ◽  
Plant Roots ◽  
Wheat Roots ◽  
Wheat Seedlings ◽  
Apoplastic Barrier ◽  
Protective Proteins

We studied the immunohistochemical localization of abscisic acid (ABA), wheat germ agglutinin (WGA) and dehydrins in the roots of wheat seedlings (Triticum aestivum L.) during 24-epibrassinolide-pretreatment (EB-pretreatment) and PEG-induced dehydration. It was found coimmunolocalization of ABA, WGA and dehydrins in the cells of central cylinder of basal part untreated and EB-pretreated roots of wheat seedlings under normal conditions and under osmotic stress. Such mutual localization ABA and protective proteins, WGA and dehydrins, indicates the possible effect of their distribution in the tissues of EB-pretreated wheat roots during dehydration on the apoplastic barrier functioning, which apparently contributes to decrease the water loss under dehydration. Perhaps, the significant localization of ABA and wheat lectin in the metaxylem region enhances EB-induced transport of ABA and WGA from roots to shoots under stress. It can be assumed that brassinosteroids can serve as intermediates in the realization of the protective effect of WGA and wheat dehydrins during water deficit.

scholarly journals Remodeling of root morphology by CuO and ZnO nanoparticles: effects on drought tolerance for plants colonized by a beneficial pseudomonad

Botany ◽  
2018 ◽  
Vol 96 (3) ◽  
pp. 175-186 ◽  
Author(s):  
Kwang-Yeol Yang ◽  
Stephanie Doxey ◽  
Joan E. McLean ◽  
David Britt ◽  
Andre Watson ◽  
...  
Keyword(s):  
Calcareous Soils ◽  
Root Hairs ◽  
Triticum Aestivum L ◽  
Metal Stress ◽  
Root Tip ◽  
Zno Nps ◽  
Wheat Seedlings ◽  
Cuo Nps ◽  
Expression Of Genes ◽  
Induced Tolerance

Formulations that include nanoparticles of CuO and ZnO are being considered for agricultural applications as fertilizers because they act as sources of Cu or Zn. Currently, few studies of the effects of these nanoparticles (NPs) consider the three-way interactions of NPs with the plant plus its microbiome. At doses that produced root shortening by both nanoparticles (NPs), CuO NPs induced the proliferation of elongated root hairs close to the root tip, and ZnO NPs increased lateral root formation in wheat seedlings (Triticum aestivum L.). These responses occurred with roots colonized by a beneficial bacterium, Pseudomonas chlororaphis O6 (PcO6), originally isolated from roots of wheat grown under dryland farming in calcareous soils. The PcO6-induced tolerance to drought stress in wheat seedlings was not impaired by the NPs. Rather, growth of the PcO6-colonized plants with NPs resulted in systemic increases in the expression of genes associated with tolerance to water stress. Increased expression in the shoots of other genes related to metal stress was consistent with higher levels of Cu and Zn in PcO6-colonized shoots grown with the NPs. This work demonstrates that plants grown with CuO or ZnO NPs showed cross-protection from different challenges such as metal stress and drought.

Sign in / Sign up

Export Citation Format

Share Document