scholarly journals Developing Hollow-Channel Gold Nanoflowers as Trimodal Intracellular Nanoprobes

2018 ◽  
Vol 19 (8) ◽  
pp. 2327 ◽  
Author(s):  
Sunjie Ye ◽  
May Wheeler ◽  
James McLaughlan ◽  
Abiral Tamang ◽  
Christine Diggle ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Single Cell Analysis ◽  
Surface Enhanced Raman Spectroscopy ◽  
Dark Field ◽  
Microscopy Imaging ◽  
Gold Nanoflowers ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Spherical Gold Nanoparticles ◽  
Low Cytotoxicity

Gold nanoparticles-enabled intracellular surface-enhanced Raman spectroscopy (SERS) provides a sensitive and promising technique for single cell analysis. Compared with spherical gold nanoparticles, gold nanoflowers, i.e., flower-shaped gold nanostructures, can produce a stronger SERS signal. Current exploration of gold nanoflowers for intracellular SERS has been considerably limited by the difficulties in preparation, as well as background signal and cytotoxicity arising from the surfactant capping layer. Recently, we have developed a facile and surfactant-free method for fabricating hollow-channel gold nanoflowers (HAuNFs) with great single-particle SERS activity. In this paper, we investigate the cellular uptake and cytotoxicity of our HAuNFs using a RAW 264.7 macrophage cell line, and have observed effective cellular internalization and low cytotoxicity. We have further engineered our HAuNFs into SERS-active tags, and demonstrated the functionality of the obtained tags as trimodal nanoprobes for dark-field and fluorescence microscopy imaging, together with intracellular SERS.

Gold Nanoflowers and Gold Nanospheres as Labels in Lateral Flow Immunoassay of Procalcitonin

2017 ◽  
Vol 13 ◽  
pp. 47-53 ◽  
Author(s):  
Kseniya V. Serebrennikova ◽  
Jeanne V. Samsonova ◽  
Alexander P. Osipov ◽  
Dulal Senapati ◽  
Denis V. Kuznetsov
Keyword(s):  
Gold Nanoparticles ◽  
Surface Enhanced Raman Spectroscopy ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Assay Sensitivity ◽  
Photometric Detection ◽  
Gold Nanoflowers ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Spherical Gold Nanoparticles

In this study, new types of nanogold labels for lateral flow immunoassay of model antigen procalcitonin based on photometric and surface-enhanced Raman scattering methods of detection were obtained. The linear range of procalcitonin determination applying gold nanoflowers as a label was between 0.5 and 10 ng/mL. The limit of photometric detection was achieved at 0.1 ng/mL that was five times lower than the sensitivity of traditional lateral flow immunoassay with spherical gold nanoparticles as a label. In addition, the conjugate of polyclonal antibodies against procalcitonin with spherical gold nanoparticles labeled with 4-mercaptobenzoic acid as Raman reporter molecule was prepared and used as an immunoprobe in lateral flow immunoassay based on surface-enhanced Raman spectroscopy to improve assay sensitivity.

Mapping gold nanoparticles on and in edible leaves in situ using surface enhanced Raman spectroscopy

RSC Advances ◽  
2016 ◽  
Vol 6 (65) ◽  
pp. 60152-60159 ◽  
Author(s):  
Zhiyun Zhang ◽  
Huiyuan Guo ◽  
Yingqing Deng ◽  
Baoshan Xing ◽  
Lili He
Keyword(s):  
Gold Nanoparticles ◽  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Mapping Technique ◽  
Raman Spectroscopic ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Spinach Leaves

A surface enhanced Raman spectroscopic (SERS) mapping technique was applied to qualitatively detect and characterize gold nanoparticles on and in spinach leaves in situ.

An ultrasensitive “turn-off” SERS sensor for quantitatively detecting heparin based on 4-mercaptobenzoic acid functionalized gold nanoparticles

2017 ◽  
Vol 9 (17) ◽  
pp. 2517-2522 ◽  
Author(s):  
Chenmeng Zhang ◽  
Xiu Liang ◽  
Tingting You ◽  
Nan Yang ◽  
Yukun Gao ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Functionalized Gold Nanoparticles ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

We develop an ultrasensitive “turn off” Surface Enhanced Raman Spectroscopy (SERS) sensor for the detection of heparin based on the anti-aggregation of 4-mercaptobenzoic acid stabilized gold nanoparticles.

Sign in / Sign up

Export Citation Format

Share Document