scholarly journals Tailored Biodegradable and Electroactive Poly(Hydroxybutyrate-Co-Hydroxyvalerate) Based Morphologies for Tissue Engineering Applications

2018 ◽  
Vol 19 (8) ◽  
pp. 2149 ◽  
Author(s):  
Luís Amaro ◽  
Daniela Correia ◽  
Teresa Marques-Almeida ◽  
Pedro Martins ◽  
Leyre Pérez ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cobalt Ferrite ◽  
Degree Of Crystallinity ◽  
Engineering Applications ◽  
Cell Proliferation And Differentiation ◽  
Cobalt Ferrites ◽  
Physico Chemical ◽  
Proliferation And Differentiation ◽  
The Degree Of Crystallinity ◽  
Composite Samples

Polymer-based piezoelectric biomaterials have already proven their relevance for tissue engineering applications. Furthermore, the morphology of the scaffolds plays also an important role in cell proliferation and differentiation. The present work reports on poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV), a biocompatible, biodegradable, and piezoelectric biopolymer that has been processed in different morphologies, including films, fibers, microspheres, and 3D scaffolds. The corresponding magnetically active PHBV-based composites were also produced. The effect of the morphology on physico-chemical, thermal, magnetic, and mechanical properties of pristine and composite samples was evaluated, as well as their cytotoxicity. It was observed that the morphology does not strongly affect the properties of the pristine samples but the introduction of cobalt ferrites induces changes in the degree of crystallinity that could affect the applicability of prepared biomaterials. Young’s modulus is dependent of the morphology and also increases with the addition of cobalt ferrites. Both pristine and PHBV/cobalt ferrite composite samples are not cytotoxic, indicating their suitability for tissue engineering applications.

scholarly journals Tailored Biodegradable and Electroactive Poly(hydroxybutyrate-co-hydroxyvalerate) Based Morphologies for Tissue Engineering Applications

2018 ◽  
Author(s):  
Luís Amaro ◽  
Daniela M. Correia ◽  
Teresa Marques-Almeida ◽  
Pedro M. Martins ◽  
Leyre Pérez ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Young Modulus ◽  
Degree Of Crystallinity ◽  
Engineering Applications ◽  
Cell Proliferation And Differentiation ◽  
Cobalt Ferrites ◽  
Physico Chemical ◽  
Proliferation And Differentiation ◽  
The Degree Of Crystallinity ◽  
Composite Samples

Polymer-based piezoelectric biomaterials have already proven their relevance for tissue engineering applications. Further, the morphology of the scaffolds plays also an important role in cell proliferation and differentiation. The present work reports on poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV), a biocompatible, biodegradable and piezoelectric biopolymer that has been processed in different morphologies, including films, fibres, microspheres and 3D scaffolds. Further, the corresponding magnetically active PHBV-based composites were also produced. The effect of the morphology on physico-chemical, thermal, magnetic and mechanical properties of pristine and composites samples was evaluated, as well as their cytotoxicity. It was observed that the morphology does not strongly affect the properties of the pristine samples but the introduction of cobalt ferrites induces changes in the degree of crystallinity that could affect the applicability of prepared biomaterials. Young modulus is dependent of the morphology and also increases with the addition of cobalt ferrites. Both, pristine and PHBV/cobalt ferrite composite samples are no cytotoxic, indicating their suitability for tissue engineering applications.

Study of structural and morphological changes during acсelerated oxidative degradation of mixtures of polyoxybutyrate with polycaprolactane

2021 ◽  
Vol 5 ◽  
pp. 60-69
Author(s):  
V. N. Vasilets ◽  
◽  
A. P. Pankina ◽  
E. A. Nemets ◽  
V. Yu. Belov ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Oxidative Degradation ◽  
Initial Mixture ◽  
Polymer Composite Material ◽  
Degree Of Crystallinity ◽  
Fenton Reagent ◽  
Dsc Method ◽  
The Degree Of Crystallinity ◽  
Composite Samples ◽  
Scanning Electron

Structural and morphological changes in the films of poly(oxybirutyrate-co-oxyvalerate) (P(OB-OV)) with poly-e-caprolactone (PCL) were studied by IR spectroscopy, DSC, and scanning electron microscopy during accelerated oxidative degradation under tests in Fenton reagent. It is shown by the DSC method that the phase separation of the P(OB-OV) and PCL components is observed in the initial mixture. The melting of various phases of P(OB-OV) is observed at temperatures of 147 °C and 157 °C, and PCL melts at a temperature of 61 °C. The degree of crystallinity of P(OB-OV) and PCL in the mixture is 67 % and 50 %, respectively. It was found that the degradation of poly(oxybirutyrate-co-oxyvalerate) prevails during incubation of composite samples in Fenton solution for 2 to 12 weeks. By changing the ratio of the components, it is possible to significantly change the rate of oxidative degradation, the molecular weight and the degree of crystallinity of the polymer composite material P(OB-OV):PCL.

scholarly journals Biomaterial technology for tissue engineering applications

2009 ◽  
Vol 6 (suppl_3) ◽  
Author(s):  
Yasuhiko Tabata
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Cell Biology ◽  
Basic Research ◽  
Local Environment ◽  
Engineering Applications ◽  
Cell Potential ◽  
Research Fields ◽  
Genetically Engineer ◽  
Proliferation And Differentiation

Tissue engineering is a newly emerging biomedical technology and methodology to assist and accelerate the regeneration and repairing of defective and damaged tissues based on the natural healing potentials of patients themselves. For the new therapeutic strategy, it is indispensable to provide cells with a local environment that enhances and regulates their proliferation and differentiation for cell-based tissue regeneration. Biomaterial technology plays an important role in the creation of this cell environment. For example, the biomaterial scaffolds and the drug delivery system (DDS) of biosignalling molecules have been investigated to enhance the proliferation and differentiation of cell potential for tissue regeneration. In addition, the scaffold and DDS technologies contribute to develop the basic research of stem cell biology and medicine as well as obtain a large number of cells with a high quality for cell transplantation therapy. A technology to genetically engineer cells for their functional manipulation is also useful for cell research and therapy. Several examples of tissue engineering applications with the cell scaffold and DDS of growth factors and genes are introduced to emphasize the significance of biomaterial technology in new therapeutic and research fields.

scholarly journals Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering

2019 ◽  
Vol 23 (1) ◽  
Author(s):  
Cen Chen ◽  
Xue Bai ◽  
Yahui Ding ◽  
In-Seop Lee
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Electrical Stimulation ◽  
Intracellular Signaling ◽  
Cell Alignment ◽  
Mechanism Study ◽  
Regeneration Medicine ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Underlying Mechanisms

AbstractRecently, electrical stimulation as a physical stimulus draws lots of attention. It shows great potential in disease treatment, wound healing, and mechanism study because of significant experimental performance. Electrical stimulation can activate many intracellular signaling pathways, and influence intracellular microenvironment, as a result, affect cell migration, cell proliferation, and cell differentiation. Electrical stimulation is using in tissue engineering as a novel type of tool in regeneration medicine. Besides, with the advantages of biocompatible conductive materials coming into view, the combination of electrical stimulation with suitable tissue engineered scaffolds can well combine the benefits of both and is ideal for the field of regenerative medicine. In this review, we summarize the various materials and latest technologies to deliver electrical stimulation. The influences of electrical stimulation on cell alignment, migration and its underlying mechanisms are discussed. Then the effect of electrical stimulation on cell proliferation and differentiation are also discussed.

A review on 3D printing in tissue engineering applications

2022 ◽  
Vol 0 (0) ◽  
Author(s):  
Mohan Prasath Mani ◽  
Madeeha Sadia ◽  
Saravana Kumar Jaganathan ◽  
Ahmad Zahran Khudzari ◽  
Eko Supriyanto ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Cell Adherence ◽  
Engineering Applications ◽  
Proliferation And Differentiation ◽  
3D Printed ◽  
Printing Techniques

Abstract In tissue engineering, 3D printing is an important tool that uses biocompatible materials, cells, and supporting components to fabricate complex 3D printed constructs. This review focuses on the cytocompatibility characteristics of 3D printed constructs, made from different synthetic and natural materials. From the overview of this article, inkjet and extrusion-based 3D printing are widely used methods for fabricating 3D printed scaffolds for tissue engineering. This review highlights that scaffold prepared by both inkjet and extrusion-based 3D printing techniques showed significant impact on cell adherence, proliferation, and differentiation as evidenced by in vitro and in vivo studies. 3D printed constructs with growth factors (FGF-2, TGF-β1, or FGF-2/TGF-β1) enhance extracellular matrix (ECM), collagen I content, and high glycosaminoglycan (GAG) content for cell growth and bone formation. Similarly, the utilization of 3D printing in other tissue engineering applications cannot be belittled. In conclusion, it would be interesting to combine different 3D printing techniques to fabricate future 3D printed constructs for several tissue engineering applications.

Cellulose-based porous scaffold for bone tissue engineering applications: Assessment of hMSC proliferation and differentiation

2015 ◽  
Vol 104 (3) ◽  
pp. 726-733 ◽  
Author(s):  
Christian Demitri ◽  
Maria Grazia Raucci ◽  
Antonella Giuri ◽  
Vincenzo Maria De Benedictis ◽  
Daniela Giugliano ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
Engineering Applications ◽  
Proliferation And Differentiation

scholarly journals Regulation of Redox Homeostasis by Nonthermal Biocompatible Plasma Discharge in Stem Cell Differentiation

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Ying Li ◽  
Eun Ha Choi ◽  
Ihn Han
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Redox Homeostasis ◽  
Stem Cell Differentiation ◽  
Redox Biology ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Recently, a growing body of evidence has shown the role of reactive species as secondary messengers in cell proliferation and differentiation, as opposed to the harmful metabolism byproducts that they were previously solely recognized as. Thus, the balance of intracellular reduction-oxidation (redox) homeostasis plays a vital role in the regulation of stem cell self-renewal and differentiation. Nonthermal biocompatible plasma (NBP) has emerged as a novel tool in biomedical applications. Recently, NBP has also emerged as a powerful tool in the tissue engineering field for the surface modification of biomaterial and the promotion of stem cell differentiation by the regulation of intracellular redox biology. NBP can generate various kinds of reactive oxygen species (ROS) and reactive nitrogen species (RNS), which may play the role of the second passenger in the cell signaling network and active antioxidant system in cells. Herein, we review the current knowledge on mechanisms by which NBP regulates cell proliferation and differentiation through redox modification. Considering the importance of redox homeostasis in the regulation of stem cell differentiation, understanding the underlying molecular mechanisms involved will provide important new insights into NBP-induced stem cell differentiation for tissue engineering.

Solid Freeform Fabrication of Polycaprolactone∕Hydroxyapatite Tissue Scaffolds

2008 ◽  
Vol 130 (2) ◽  
Author(s):  
L. Shor ◽  
S. Güçeri ◽  
M. Gandhi ◽  
X. Wen ◽  
W. Sun
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Structural Integrity ◽  
Solid Freeform Fabrication ◽  
Porous Scaffolds ◽  
Freeform Fabrication ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Bone tissue engineering is an emerging field providing viable substitutes for bone regeneration. Freeform fabrication provides an effective process tool to manufacture scaffolds with complex shapes and designed properties. We developed a novel precision extruding deposition (PED) technique to fabricate composite polycaprolactone∕hydroxyapatite (PCL∕HA) scaffolds. 25% concentration by weight of HA was used to reinforce 3D scaffolds. Two groups of scaffolds having 60% and 70% porosities and with pore sizes of 450μm and 750μm respectively, were evaluated for their morphology and compressive properties using scanning electron microscopy and the mechanical testing. In vitro cell-scaffold interaction study was carried out using primary fetal bovine osteoblasts. The cell proliferation and differentiation were evaluated by Alamar Blue assay and alkaline phosphatase activity. Our results suggested that compressive modulus of PCL∕HA scaffold was 84MPa for 60% porous scaffolds and was 76MPa for 70% porous scaffolds. The osteoblasts were able to migrate and proliferate for the cultured time over the scaffolds. Our study demonstrated the viability of the PED process to fabricate PCL scaffolds having necessary mechanical property, structural integrity, controlled pore size, and pore interconnectivity desired for bone tissue engineering.

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